19-11-2017

HYPERSPECTRAL IMAGE ANALYSIS

José Manuel Amigo

University of Copenhagen, Denmark
Federal University of Pernambuco, Brazil
jmar@life.ku.dk
www.hypertools.org / www.models.life.ku.dk

CAT – Chemometric Analytical Technologies

Key Areas
- Metabolomics and bio-spectroscopy
- Molecular functionality and health effects of plant foods
- Spectroscopic Quality Control and Process Analytical Technology

Key Tools
- Chemometrics
- Physical analysis of food (texture, viscosity, etc)
- Chromatography
- Olfactometry
- Optical spectroscopy and nuclear magnetic resonance

FOOD, U. Copenhagen, Denmark. www.models.life.ku.dk www.hypertools.org jmar@life.ku.dk

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CAT – Chemometric Analytical Technologies

Nuclear Magnetic Resonance Spectroscopy
- Bruker 600 MHz spectrometer equipped with cryo-probe and sampleJet
- 2 Bruker 500 MHz spectrometer equipped with a sample exchanger
- Bruker 400 MHz spectrometer equipped with HR-MAS and CP-MAS probes
- Resonance Instruments 23 MHz benchtop relaxometry

Spectroscopy
- InfraRed - (Perkin Elmer System 2000 FT-IR spectrometer; Bomen FT-IR spectrometer with Diamond ATR; FOSS
WineScan interferometer)
- Near InfraRed - (Bomen FT-NIR with vpl fiber probe; FOSS NIRSystems 6500 (FOSS), FOSS NIT FoodScan; portable NIR
with fiber probe)
- Raman - (Perkin Elmer System 2000 FT-Raman spectrometer; Portable Raman with fiber probe; Kaiser laser Raman
microscope)
- Multi-dimensional fluorescence
- DAD-UV-Vis

Chromatography
- 3 GC-MS Spectrometers for aroma analysis
- 1 GC-MS with sampling station for metabolomics
- 2 HPLC

Imaging
- High resolution microscopy
- Vis-NIR imaging
- Raman microscope
- NIR-HSI sysmtem. Line mapping
- In-house digital imaging systems
FOOD, U. Copenhagen, Denmark. www.models.life.ku.dk www.hypertools.org jmar@life.ku.dk

CAT – Chemometric Analytical Technologies

Chemometrics

- It is difficult to say something specific. We do everything.

CHEMOMETRICS FOR SOLVING PROBLEMS!!!

PAT, QbD, MSPC

Understanding LIFE
Multi-way analysis  PARAFAC, PARAFAC2, TUCKER3
Design of Experiments
Developing new methods in Metabolomics and Environmental monitoring
Calibration (development, transfer, etc)
Multivariate Statistics (Metabolomics)
Imaging  Digital, hyperspectral, etc…
Filtering of signals

MODELLING EVERYTHING!!!

FOOD, U. Copenhagen, Denmark. www.models.life.ku.dk www.hypertools.org jmar@life.ku.dk

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CAT – Chemometric Analytical Technologies

Visit our website! www.models.life.ku.dk

And also HYPER-Tools website!!! www.hypertools.org

FOOD, U. Copenhagen, Denmark. www.models.life.ku.dk www.hypertools.org jmar@life.ku.dk

A little about me

Sestao
José Manuel Amigo Rubio
I am from Sestao, Basque country, Spain

Bachelor degree in 2001. Analytical Chemistry. Basque Country

PhD degree in Chemistry in 2007. Barcelona
Post-doctoral student from 2007 to 2010. Copenhagen

FOOD, U. Copenhagen, Denmark. www.models.life.ku.dk www.hypertools.org jmar@life.ku.dk

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A little about me

Sestao
José Manuel Amigo Rubio
I am from Sestao, Basque country, Spain

Associate Professor at CAT-UCPH. Copenhagen. Denmark

Guest Professor at LAC-UFPE. Recife. Brazil

FOOD, U. Copenhagen, Denmark. www.models.life.ku.dk www.hypertools.org jmar@life.ku.dk

A little about me

Multi-way analysis in Chromatography

Hyperspectral and digital Imaging

Environmental monitoring
Education in Chemometrics

FOOD, U. Copenhagen, Denmark. www.models.life.ku.dk www.hypertools.org jmar@life.ku.dk

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A little about me

Hyperspectral open projects

Pharmaceutical production: Blending moitoring, final tablet homogeneity, dry-granulation monitoring
(Takeda, Novo Nordisk, U. Copenhagen, U. Pernambuco)
Food quality control: Cheese manufacturing, fruits damages, bread stalingness, and more... (Arla,
Novozymes, U. Pernambuco, U. Milano, U. Foggia, IVIA, Cafe Molinari, Ferrero, etc.)
Plastic sorting (INNOSORT)
Forensic sciences: Explosives detection in hands, biological samples in tissues, inks in document counterfeits,
MOFs in shot guns (U. Alcala de Henares, U. Pernambuco, Policia Federal Brazil)
New/old development: Filter/LED-based technologies (myself )

Digital imaging open projects

CT imaging: Detection of lung tumors (U. Copenhagen)
RGB: Diseases in skin, fruits ripening, fruits classification (Leo Pharma, U. Milano, U. Pernambuco)
X-Ray: Detection of pelvis and malformities (U. Copenhagen)
CLMS: Structure in yogurt (U.Copenhagen)

FOOD, U. Copenhagen, Denmark. www.models.life.ku.dk www.hypertools.org jmar@life.ku.dk

Target of the course

Main target of this course  YOU

YOU are the most important part of this course
What about...
Spectroscopy? Linear Algebra?
Matlab? Chemometrics?

What would you like to know after these 5 days?

FOOD, U. Copenhagen, Denmark. www.models.life.ku.dk www.hypertools.org jmar@life.ku.dk

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Target of the course

Secondary targets
How to solve problems derived from hyperspectral images.

Main tools: Our minds and logical thinking

FOOD, U. Copenhagen, Denmark. www.models.life.ku.dk www.hypertools.org jmar@life.ku.dk

Target of the course

Secondary targets
How to solve problems derived from hyperspectral images.

Main tools: Our minds and logical thinking

Who is happy? Who is bigger? Who is sad?

Who is green?

How can we tell this to a computer???

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FOOD, U. Copenhagen, Denmark. www.models.life.ku.dk www.hypertools.org jmar@life.ku.dk

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Target of the course

Secondary targets
How to solve problems derived from hyperspectral images.

Main tools: Our minds and logical thinking

FOOD, U. Copenhagen, Denmark. www.models.life.ku.dk www.hypertools.org jmar@life.ku.dk

Target of the course

Secondary targets
How to solve problems derived from hyperspectral images.

Main tools: Our minds and logical thinking

Adequation of the cameras to our problems

FOOD, U. Copenhagen, Denmark. www.models.life.ku.dk www.hypertools.org jmar@life.ku.dk

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Target of the course

Secondary targets
How to solve problems derived from hyperspectral images.

Main tools: Our minds and logical thinking

Adequacy of our knowledge to the problem

FOOD, U. Copenhagen, Denmark. www.models.life.ku.dk www.hypertools.org jmar@life.ku.dk

Timetable

Beer time???

FOOD, U. Copenhagen, Denmark. www.models.life.ku.dk www.hypertools.org jmar@life.ku.dk

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Timetable

Material
Handouts of all the slides

HYPER-Tools working on Matlab (version 2014b and further)

Samples

Many references (manuscripts and book chapters)

Your minds and curiosity

FOOD, U. Copenhagen, Denmark. www.models.life.ku.dk www.hypertools.org jmar@life.ku.dk