QUESTION 1

1. Which of the following would be the positive reaction for the Venereal Disease Research
Laboratory Test
Diffused RBC occupying the entire micro
titer well

Macroscopic appearance of clumps

Macroscopic appearance of black charcoal

Microscopic Appearance of clumps

1 points

QUESTION 2
1. Cholesterol is added to the antigen in flocculation tests for syphilis to:
Destroy tissue impurities present in the alcoholic beef
extract

Sensitize the sheep RBCs

Decrease specificity of the antigen

Increase the sensitivity of the antigen

1 points

QUESTION 3
1. The rapid plasma reagin assay for syphilis does not need to be read microscopically
because the antigen is
Cardiolipin

Complexed with
latex

Complexed with
charcoal
 Inactivated bacterial
cells

1 points

QUESTION 4
1. The Venereal Disease Research Laboratory (VDRL) test for syphilis is classified as a(n)
Agglutination reaction

Flocculation reaction

Hemagglutination
reaction

Precipitation reaction

1 points

QUESTION 5
1. One cause of a false-positive VDRL test is
Brucellosis

Treponema pallidum
infection

Rocky Mountain spotted

fever

Systemic lupus
erythematosus

1 points

QUESTION 6
1. Which of the following is an example of a treponemal antigen test used for the diagnosis
of syphilis?
 CRP

RPR

VDR
L

FTA-
A
B
S

1 points

QUESTION 7
1. Which of the following is an acute phase protein that binds to the membrane of certain
microorganisms and activates the complement system?
C-Reactive
Protein

C3b

Factor I

Platelet
Factors

Calcium

1 points

QUESTION 8
1. Which complement protein is present in the greatest concentration in human serum?
C

C
 C

1 points

QUESTION 9
1. Which of the following assay would be classified as a Functional Assay for Complement
system?
Radial Immunodiffusion for
C3

Hemolytic Assay

Assay for C4 concentration

Assay for Factor B

Concentration

1 points

QUESTION 10
1. Which of the following is the most important in the interpretation of agglutination tests for
diseases like Typhoid Fever, Brucellosis and Tularemia?
History of previous infection

Rise in titer of the patient's serum

Naturally occurring antibodies prevalent where the disease is

endemic

None of the Choices

1 points

QUESTION 11
1. Which of the following is most useful in establishing a diagnosis in the convalescence
phase of viral infection?
Slide culture

Serological
Techniques

Shell Vial

Silver Staining
techniques

1 points

QUESTION 12
1. What is the indicator system used in the complement fixation test?
Sensitized sheep red blood cells

Fluorescent-labeled antihuman
globulin

Enzyme-labeled antihuman
globulin

Guinea pig complement

1 points

QUESTION 13
1. Diagnosis of Streptococcus pyogenes infection is indicated by the presence of
Anti-protein
A

Anti-DNaseB

Anti-beta-toxi
n
 C-reactive
protein

1 points

QUESTION 14
1. The Streptolysin O Control Tube in an ASO hemolytic assay exhibits no cell lysis. What
is the most likely explanation for this?
Incorrect pH of the
buffer

Low ionic strength

buffer

Oxidation of the
enzyme

Failure to dilute the

serum

1 points

QUESTION 15
1. An ASO titer and a Streptozyme test are performed on a patient's serum. The ASO titer
was negative, showing hemolysis in all patient tubes. The Streptozyme test is positive, and both
the positive and negative controls react appropriately. What can you conclude from these test
results?
The patient has a high titer of ASO.

The patient has an antibody to a streptococcal exoenzyme other than

streptolysin O.

The patient has not had a previous streptococcal infection.

The patient has scarlet fever.

1 points
QUESTION 16
1. Which of the following applies to acute rheumatic fever?
Symptoms begin after either a throat or a skin infection.

Antibodies to group A streptococci cross-react with heart

tissue.

Diagnosis is usually made by culture of the organism.

All patients suffer permanent disability.

1 points

QUESTION 17
1. What is the principle used for Widal Test and Weil Felix test?
Coaagglutination

Passive
Agglutination

Direct
Agglutination

Agglutination
Inhibition

1 points

QUESTION 18
1. A positive reaction for ASO Slide Latex Test is:
Diffused RBC occupying the entire micro
titer well

Macroscopic appearance of clumps

Macroscopic appearance of black charcoal

Microscopic Appearance of clumps

 1 points

QUESTION 19
1. In a positive anti-streptolysin "O" enzyme inhibition test, the patient's:
Streptolysin "O" enzyme in the patient serum neutralizes the anti-streptolysin "O" reagent,
resulting in no hemolysis

Red Blood Cells are hemolyzed by the streptolysin "O" enzyme in the reagent

Anti streptolysin "O" neutralizes the streptolysin "O" reagent, resulting in hemolysis

Anti streptolysin "O" inhibits the reagent streptolysin "O"s, resulting in no hemolysis

1 points

QUESTION 20
1. In the direct fluorescent antibody test for primary syphilis, spirochetes are detected by
addition of labeled antibody to?
Treponema pallidum

Cardiolipin

Human immunoglobulin

Non Pathogenic
Treponemes