NAME: Aiman H.

Asnalul__ _________________ ___ GROUP NUMBER: _________

YEAR AND SECTION: CCR3-B3 _____________________ DATE PERFORMED: 05/09/2022
PROFESSOR: MA’AM DEOGRACIAS, MARY ANN Q. DATE CORRECTED: ________

EXPERIMENT 1
THE COMPOUND MICROSCOPE

I. OBJECTIVE: TO STUDY THE PARTS AND THE CORRECT TECHNIQUE OF USING THE
COMPOUND MICROSCOPE.
II. MATERIALS: compound microscope, glass slides with specimen

III. DISCUSSION: The compound microscope is an example of a light microscope, a type that
makes use of visible light. It consists of two sets of lenses, namely, the primary magnifiers or
the objectives, the lenses of which are immediately next to the specimen being studied; and the
secondary magnifier, that is, the ocular or eyepiece, the lenses of which are next to the eye.

IV. PROCEDURE: Draw and label the parts of the compound microscope.
V. OBSERVATION/CONCLUSION:

1. Give the function(s) of the following:

a. ocular/eyepiece – The eyepieces are the lenses at the top that the viewer looks
through; they are usually 10X or 15X. To get the total magnification level, multiply the
magnification of the objective used (ex: 10X eyepiece * 40X objective = 400X total
magnification).

b. revolving nosepiece – This circular structure is where the different objective lenses
are screwed in. To change the magnification power, simply rotate the turret.

c. coarse adjustment knob – A rapid control which allows for quick focusing by moving
the objective lens or stage up and down. It is used for initial focusing.

d. low power objective –  cover a wide field of view and they are useful for examining large
specimens or surveying many smaller specimens. This objective is useful for aligning the
microscope. The power for the low objective is 10X. Place one of the prepared slides onto the
stage of your microscope.

e. high power objective –  is ideal for observing fine details within a specimen sample. The
total magnification of a high-power objective lens combined with a 10x eyepiece is equal to 400x
magnification, giving you a very detailed picture of the specimen in your slide.

f. stage – The flat platform that supports the slides. Stage clips hold the slides in place.
If your microscope has a mechanical stage, the slide is controlled by turning two knobs
instead of having to move it manually. One knob moves the slide left and right, the other
moves it forward and backward.

g. condenser – it gives you control over how the substage lighting is projected onto the
specimen gives you control over how the substage lighting is projected onto the specimen

h. mirror – it is used to reflect light from an external light source up through the bottom of the
stage.

i. iris diaphragm – iris is located under the stage and is an apparatus that can be
adjusted to vary the intensity, and size, of the cone of light that is projected through the
slide.
 2. Describe briefly and in a systematic order, the manner or method of properly
using the compound microscope.

- The systematic order of properly using a microscope starts with the handling. You
should carry it with both hands with one hand supporting the base. When you put the
microscope down, put your specimen on the slide and fasten it. Look through the eye
piece and adjust the lens as low as possible but without touching the slide

3. Give some precautionary measures in the use of the microscope.

 Do not touch the glass part of the lenses with your fingers. Use only special lens
paper to clean the lenses.
 Always keep your microscope covered when not in use.
 Always carry a microscope with both hands. Grasp the arm with one hand and
place the other hand under the base for support.

4. Name the three general parts of the compound microscope and their sub-parts.

Head: The upper part of the microscope houses the eyepiece and objective lenses.

Base: The bottom of the microscope—what the microscope stands on.

Arm: Structural element that connects the head of the microscope to the base.