ACADEMIC PLANNER & UNITIZATION OF SYLLABUS

ACADEMIC YEAR 2021-22

DEPARTMENT: BIOTECHNOLOGY CLASS: I SEM (NEP)
SUBJECT: BIOTECHNOLOGY
PAPER: CELL BIOLOGY & GENETICS (NEP Core)

MONTH/YEAR WEEK CLASS PORTIONS FACULTY

1 Introduction to biotechnology: Definition GK

and objectives.
OCTOBER 2 Bridging the gap: Connecting PUC BMA
4 biology to degree biotechnology.
2021 3 Introduction to biotechnology: GK
Applications & scope.
4 Introduction of the syllabus. BMA

1 Unit 3: Genetics: Introduction, Mendel GK

1 and his works.
2 Unit 1: Cell biology: Introduction. BMA
1 Unit 3: Genetics: Laws of inheritance- GK
Dominance, Law of Segregation.
2 Unit 3: Genetics: Laws of inheritance- GK
Law of Segregation. Incomplete
2 dominance & Codominance.
3 Unit 1: Cell biology: Discovery of cell. BMA

4 Unit 1: Cell biology: Cell theory BMA

NOVEMBER
1 Unit 3: Law of independent assortment. GK
2021
2 Unit 3: Back cross & Test cross with examples. GK
3
3 Unit 1: Cell biology: Ultrastructure of BMA
eukaryotic cell- Animal cell
4 Unit 1: Cell biology: Ultrastructure o f plant BMA
cell.

1 Unit 3: Gene interaction: Supplementary GK

factors - comb pattern in fowls
2 Unit 3: Gene interaction: Complementary GK
4
genes- flower colour in sweet peas.
3 Unit 1: Cell biology: Structure and functions of BMA
cell wall.
4 Unit 1: Cell biology: Structural BMA
organization of plasma membrane.
MONTH/YEAR WEEK CLASS PORTIONS FACULTY

1 Unit 1: Functions of plasma membrane. BMA

2 Unit 2: Cell organelles: Structure and BMA

functions of Mitochondria
1 Unit 3: Gene interaction: Multiple GK
allelism- blood groups in man, multiple
factors-skin colour in man.
2
2 Unit 3: gene interaction: Epistasis- plumage GK
colour in poultry.

3 Unit 1: Structure and functions of BMA

endoplasmic reticulum.

4 Unit 1: Structure & functions of Cytosol, BMA

ribosomes and lysosome.

1 Unit 3: Maternal inheritance: Kappa particle GK

DECEMBER
in Paramoecium
2021 2 Unit 3: Maternal inheritance: Plastid GK
3 inheritance in Mirabilis & Petite characters
in yeast.
3 Unit 1: Structure & functions of Golgi BMA
complex, peroxisomes & vacuole.

4 Unit 1: Structure & functions of Chloroplast. BMA

4
IA TEST

1 Unit 3: Sex-linked inheritance GK

5 2 Unit 3: Chromosomal theory of inheritance GK

3 Unit 1: Structure & functions of Nucleus, BMA

nucleolous and cytoskeletal structures.
1 Unit 4: Linkage: general introduction. GK
Coupling & repulsion hypothesis
2 Unit 4: Linkage in Maize & Drosophila. GK
JANUARY 1
3 Unit 2: Chromosomes: General BMA
2022 introduction, discovery & morphology
4 Unit 2: Structural organization of BMA
metaphase chromosome.
MONTH/YEAR WEEK CLASS PORTIONS FACULTY

1 Unit 4: Mechanism of crossing over & GK

chromosome mapping.

2 Unit 4: Structural and numerical GK

2 chromosomal aberrations.

3 Unit 2: Ultrastructure of chromosome- BMA

hypothesis, folded-fibre & nucleosome
models.

4 Unit 2: Salivary gland chromosome & BMA

Lampbrush chromosomes.
1 Unit4: Sex determination in plants and GK
animals- XX-XY, XX-XO, ZW-ZZ, ZO-ZZ types.
2 Unit4: Mutations: Introduction. Types of GK
3 mutations- spontaneous mutation.
3 Unit2: Cell cycle: phases of cell cycle & BMA
JANUARY checkpoints of cell cycle.
4 Unit 2: Cell cycle- enzymes involved, BMA
2022
achromatic apparatus & significance.
1 Unit 4: Mutations: Induced mutation. GK
Physical & chemical mutagens.
2 Unit4: Mutations in plants, animals & GK
4 microbes and their merits & demerits.
3 Unit2: Senescence & programmed cell death. BMA

4 Unit2: Senescence & programmed cell death. BMA

5 1 Discussion of Question bank GK

 ACADEMIC PLANNER & UNITIZATION OF SYLLABUS
ACADEMIC YEAR 2021-22
DEPARTMENT: BIOTECHNOLOGY CLASS: III SEM
SUBJECT: BIOTECHNOLOGY
PAPER: MOLECULAR BIOLOGY & BIOPHYSICS

MONTH/YEAR WEEK CLASS PORTIONS FACULTY

2 1 GK
Syllabus & reference books discussion.

1 Bridging III sem biochemistry with BMA

Molecular biology by revision.
3
2 GK
Unit 2.1: Transcription: Central Dogma

OCTOBER 1 BMA
Unit 1.1: Characteristics of genetic material
2021 2 GK
Unit 2.1: Genetic code, Codon dictionary.

4 3 Unit 1.1: Experiments to prove of DNA as BMA

genetic material.
4 Unit 1.1: Experiments to prove of RNA as BMA
genetic material.
5 Unit 2.1: Genetic code; properties and GK
Wobble hypothesis
1 Unit 2.1: Transcription in prokaryotes: GK
Introduction & Promoters.
1 Unit 2.1: Transcription in prokaryotes: GK
2 RNA polymerase & direction of
transcription.
1 BMA
Unit 1.1: DNA-structure and chemical nature

2 Unit 2.1: Transcription in prokaryotes: GK

2 Mechanism.
3 Unit 2.1: Transcription in eukaryotes: GK
NOVEMBER
Promoters & polymerases.
2021 4 BMA
Unit 1.1: DNA-different forms and functions.

1 Unit 1.1: RNA-structure and functions. GK

2 Unit 2.1: Transcription in eukaryotes - BMA

3 Mechanism.
3 Unit 2.1: Post transcriptional GK
modifications of eukaryotic mRNA.
4 Unit 1.2: Concept of gene: prokaryotic gene. BMA
MONTH/YEAR WEEK CLASS PORTIONS FACULTY

1 Unit 1.2: Concept of gene: eukaryotic gene, BMA

introns and exons.
2 Unit 2.2: Translation: Introduction, GK
4 Structure of prokaryote and eukaryote
ribosomes.
3 Unit 2.2: Translation: Enzymes and GK
factors involved in translation.
4 Unit 1.3: Replication in prokaryotes & BMA
eukaryotes–introduction. Enzymes
involved in replication.
1 Unit 1.3: Replication: Mechanism of BMA
replication.
2 Unit 2.2: Translation in Prokaryotes – Steps: GK
1 Activation aminoacids and acylation of tRNA.
3 Unit 2.2: Translation in prokaryotes; GK
Initiation of polypeptide chain and chain
elongation.
4 Unit 1.3: Replication: Models of replication- BMA
Theta model & rolling circle model.
1 Unit 1.3: Replication: Models of replication- BMA
semi conservative model.
2 Unit 2.2: Translation in prokaryotes- GK
2 termination of polypeptide chain.
DECEMBER
3 Unit 2.2: Translation in eukaryotes- GK
2021 mechanism and steps.

4 Unit 1.3: Replication: Differences between BMA

prokaryotic and eukaryotic replication.

2
3 IA TEST
3

1 Unit 1.4: DNA repair: Causes of DNA damage. BMA

2 Unit 2.2: Translation: Brief account of GK

4 protein folding and modifications.
3 Unit 3: Regulation of gene expression: GK
Introduction
4 Unit 1.4: DNA repair: Mechanism of repair- BMA
photo reactivation, excision repair.
MONTH/YEAR WEEK CLASS PORTIONS FACULTY

1 Unit 1.4: DNA repair: Mechanism of repair- BMA

mis-match reactivation and SOS repair.
2 Unit 3.1: Gene regulation in prokaryotes: GK
1 Transcriptional control mechanism.
3 Unit 3.1: Gene regulation in prokaryotes: GK
negative control and positive control.
4 Unit 1.5: DNA recombination: BMA
Transformation in prokaryotes.
1 Unit 1.5: DNA recombination: BMA
Transformation in prokaryotes.
2 Unit 3.1: Gene regulation in prokaryotes: GK
2 Operon concept- Lac Operon.
3 Unit 3.1: Gene regulation in prokaryotes: GK
JANUARY
Operon concept- tryptophan Operon.
2022 4 Unit 1.5: Conjugation & Transduction in BMA
prokaryotes.
1 Unit 4.2: Spectroscopy: Beer-Lambert’s Laws BMA
Principles & applications of Colorimetry.

2 Unit 3.2: Gene regulation in prokaryotes: GK

3 Transcriptional activation.
3 Unit 3.2: Gene regulation in prokaryotes: GK
Galactose metabolism in yeast.

4 Unit 4.2: Spectroscopy: UV & Visible BMA

spectrophotometry, Absorption
spectroscopy.
1 Unit 4.2: Spectroscopy: fluorescence BMA
spectroscopy, X-ray crystallography & NMR
imaging.
4 2 Unit 3.3: General account of Insertional GK
elements and transposons. Transposable
elements in Maize & Drosophila.
3 Unit 3.4: Gene organization & expression in GK
mitochondria & chloroplast.
4 Unit 4.3: Principles and applications of BMA
Chromatography-Paper, TLC & adsorption.
1 Unit 4.3: Principles and applications of BMA
chromatography-affinity, Ion exchange &
FEBRUARY HPLC.
2022 1 2 Unit4.1: Basics of biophysics, pH & buffer GK
concepts.
3 Unit 4.2: Chemical bonds stabilizing GK
biomolecules.
4 Unit 4.4: Centrifugation: Basic principles, BMA
types and applications.
 ACADEMIC PLANNER & UNITIZATION OF SYLLABUS

ACADEMIC YEAR 2021-22

DEPARTMENT: BIOTECHNOLOGY CLASS: V SEM
SUBJECT: BIOTECHNOLOGY
PAPER: V- GENETIC ENGINEERING & ENVIRONMENTAL BIOTECHNOLOGY

MONTH/YEAR WEEK CLASS PORTIONS FACULTY

2 1 GK
Syllabus & reference books discussion.

1 Part B: Environmental Biotechnology BMA

Unit 1: Introduction.
2 Part B: Environmental Biotechnology BMA
Unit 1: Renewable sources of energy.
3
3 Part A: Genetic Engineering (GE) GK
Unit 1: Introduction to Genetic engineering.
4 Part B: Environmental Biotechnology BMA
OCTOBER Unit 1: Renewable sources of energy.

2021 1 Part B: Unit 1: Conventional fuels and their BMA

environmental impact.
2 Part B: Unit 1: Conventional fuels and their BMA
environmental impact.
3 Part A: Unit 1: Principles of Genetic GK
4 engineering
4 Part B: Unit1: BMA
Modern fuels and their environmental impact.
5 Part A: Unit 1: Applications of genetic GK
engineering
6 Part A: Unit 1: Tools of genetic engineering- GK
introduction.
1 Part B: Unit2: Bio fertilisers and bio BMA
pesticides – introduction.
Part A: Unit 1: Tools of genetic engineering- GK
2 DNA manipulative enzymes- Restriction
1 enzymes
Part A: Unit 1: Tools of genetic engineering- GK
3 DNA manipulative enzymes- DNA Ligase,
NOVEMBER Alkaline phosphatase, Reverse transcriptase,
Terminal transferase.
2021
1 Part B: Unit2: Bio fertilisers- Role of BMA
symbiotic N2 fixing bacteria.
2 2 Part A: Unit 1: Gene cloning vectors - GK
Plasmids
3 Part A: Unit 1: Gene cloning vectors – pBR GK
322 & pUC 19
MONTH/YEAR WEEK CLASS PORTIONS FACULTY

1 Part B: Unit2: Bio fertilisers- Role of BMA

nonsymbiotic N2 fixing bacteria.
2 Part A: Unit 1: Gene cloning vectors GK
3 Bacteriophage vectors and Cosmids.
3 Part B: Unit2: Bio fertilisers- Algal and BMA
fungal bio fertilisers.
1 Part B: Unit2: Bio leaching- enrichment of BMA
ores by microorganisms.
NOVEMBER
2 Part A: Unit 2: Invitro construction of rDNA GK
2021 4 molecules – Isolation of desired DNA.
3 Part A: Unit 2: Invitro construction of rDNA GK
molecules – preparation of desired DNA.
1 Part B: Unit3: Environmental significance BMA
of GMO’s
1 Part A: Unit 2: Preparation of vector DNA,
2 GK
Restriction digestion and Ligation of desired
DNA with vector DNA.
3 Part A: Unit 2: Construction of Genomic GK
DNA libraries.
1 Part B: Unit3: Bioremediation of soil. BMA

2 2 Part B: Unit3: Bioremediation of water BMA

DECEMBER contaminated with oil spills, heavy metals and
2021 detergents.
3 Part A: Unit 2: Construction of cDNA GK
libraries.
1
3 2 IA TEST

1 Part B: Unit3: Bioremediation of water BMA

contaminated with detergents.
4
2 Part B: Unit3: Biodegradation of lignin BMA
and cellulose.
3 Part A: Unit 3: Transformation of rDNA GK
molecules into target organisms – CaCl2
method & Electroporation.
1 Part B: Unit3: Phytoremediation. BMA

JANUARY 1 2 Part B: Unit3: Degradation of pesticides & BMA

toxic chemicals.
2022 3 Part A: Unit 3: Transformation of rDNA GK
molecules into target organisms –
Microinjection method.
MONTH/YEAR WEEK CLASS PORTIONS FACULTY

1 Part B: Unit3: Degradation of aromatic & BMA

chlorinated hydrocarbons & petroleum
2
products.
2 Part A: Unit 3: Screening of recombinants – GK
colony hybridization method.
3 Part A: Unit 3: Screening of recombinants – GK
immunological method.
JANUARY
1 Part B: Unit3: Treatment of municipal BMA
2022 wastes.
3
2 Part A: Unit 3: Expression of cloned DNA in GK
E.coli.
3 Part A: Unit 4: Molecular biology techniques: GK
Electrophoresis – Nucleic acids.
1 Part B: Unit3: Treatment of industrial BMA
effluents.
4
2 Part A: Unit 4: Molecular biology techniques: GK
Electrophoresis – proteins. PCR.
3 Part A: Unit 4: Molecular biology techniques: GK
Nucleic acid sequencing & Blotting techniques.
1 Part A: Unit 4: Production of recombinant GK
insulin.
FEBRUARY 1 2 Part A: Unit 4: Production of recombinant GK
2022 Human growth hormone & HBV vaccines.
3 Old question paper discussion. GK
 ACADEMIC PLANNER & UNITIZATION OF SYLLABUS

ACADEMIC YEAR 2021-22

DEPARTMENT: BIOTECHNOLOGY CLASS: V SEM
SUBJECT: BIOTECHNOLOGY
PAPER: VI - IMMUNOLOGY & ANIMAL BIOTECHNOLOGY

MONTH/YEAR WEEK CLASS PORTIONS FACULTY

1 BMA
Syllabus & reference books discussion.
1
2 Part A: Unit 1: Immunology: Introduction, GK
history & scope.
1 Part B: Unit1: Animal biotechnology: BMA
Introduction and scope.
2 2 Part A: Unit 1: Immunity – innate and GK
acquired (active & passive) immunity.
3 GK
Part A: Unit 1: Humoral immunity.
NOVEMBER 1 Part B: Unit1: Culture media- Natural BMA
media.
2021
2 Part A: Unit 1: Cell mediated immunity. GK
3 3 Part A: Unit 1: Cells of immune response and GK
their functions.
1 Part B: Unit1: Culture media- Chemically BMA
defined media.
2 Part A: Unit 1: Organs of immune response GK
4 and their functions.
3 Part A: Unit 2: Antigens – features, types, GK
epitopes and haptens.
1 Part B: Unit1: Growth factors. BMA

1 2 Part B: Unit 2: Primary culture: Cell lines and BMA

cloning – Desegregation and isolation of tissue.
3 Part A: Unit 2: Factors influencing GK
antigenicity. Antibodies: Structure.
1 Part B: Unit2: Secondary culture – BMA
DECEMBER transformed animal cells.
2
2 Part A: Unit2: Types, properties & GK
2021
functions of immunoglobulins.
3 Part A: Unit 2: Production of antibodies. GK
1
3 2 IA TEST

3
MONTH/YEAR WEEK CLASS PORTIONS FACULTY

Part B: Unit2: Continuous cell lines. BMA

DECEMBER 4 Part A: Unit2: Blood cell components, GK

2021 ABO grouping & Rh typing.
Part A: Unit2: Antigen-Antibody reaction: GK
Precipitation & Immunoelectrophoresis.
Part B: Unit3: Transfection of animal cell BMA
lines, HAT selection & selectable markers.
1
Part A: Unit2: Antigen-Antibody reaction: GK
Heamagglutination & labelled antibody.
Part A: Unit3: Complement system: GK
introduction & features.
1 Part B: Unit3: Transplantation of cultured BMA
cells.
2
2 Part B: Unit3: Expression of cloned BMA
JANUARY proteins in animal cells.
3 Part A: Unit3: Complement system: GK
2022 Structure, components & functions.
1 Part B: Unit3: Downstream processing of BMA
the expressed proteins.
3
2 Part B: Unit4: Applications of animal cell BMA
culture: Vaccine production.
3 Part A: Unit3: Hypersensitivity & allergic GK
reactions: Type I & Type II.
1 Part B: Unit4: Part B: Unit4: Applications BMA
of animal cell culture: Monoclonal
4
antibody production..
2 Part B: Unit4: Transgenic animals: BMA
techniques.
3 Part A: Unit3: Hypersensitivity & allergic GK
reactions: Type III, IV & V.
1 Part B: Unit4: Transgenic animals: BMA
Applications of transgenic mice & sheep.
FEBRUARY 1
2 Part A: Unit4: Vaccines & immunization- BMA
2022 passive & active immunization.
3 Part A: Unit4: Types of vaccines. BMA