Methoden der Mikrobiologie
Parameter
Total aerobic plate count 30°C/ ISO 4833-1:2013; Internal
Enterobacteriaceae/10g
Escherichia coli/10g#
Enterobacter sakazakii/10 g2
Sulphite reducing clostridia/g
Staphylococcus aureus/g
Moulds/g
Yeasts/g
Bacillus cereus/g
Salmonella species/25g
Clostridium perfringens
Enterococci
Listeria monocyogenes
Bifidobacterium animalis
3/25/2020
methode of analysis ISO Norm (Referenz) ISO NORM aktuellste Version Änderungen zur Referenz Version
methode ISO 4833-1:2013 ISO 4833-1:2013 Aktuelle Version PCA
This second edition cancels and replaces the first edition (ISO 21528-2:2004), which
has been technically revised with the following main changes:
— the confirmation step has been changed by replacing glucose agar by glucose OF
medium;
— precision data based on the results of an interlaboratory study using the method
ISO 7402, 1993 Internal methode ISO 21528-2:2004 ISO 21528-2:2017 according to this revised edition has been included in an informative annex. VRBG ISO 8523:1991
ISO 8523 Internal methode ISO 7251:2005 ISO 7251:2005 Aktuelle Version
This first edition cancels and replaces ISO/TS 22964:2006, which has been
technically revised with the following changes:
— the scope has been extended to Cronobacter spp. detection in food products
for humans and feeding animals and environmental samples and the title changed
accordingly;
— the enrichment broth, modified lauryl sulfate tryptose broth (mLST), has been
replaced by Cronobacter selective broth (CSB);
— the isolation agar, Enterobacter sakazakii isolation agar (ESIA) has been
replaced by chromogenic Cronobacter isolation (CCI) agar;
— several confirmation tests have been replaced by other tests according to Table
according GB 4789.40 ISO/TS 22964:2006 ISO 22964:2017 1 of this document.
IJFM 1995; Internal methode ISO 15213:2003 ISO 15213:2003 Aktuelle Version Iron sulphite aIso 15213:2003
Gegenüber DIN EN ISO 6888-3:2003-09 wurden folgende Berichtigung
vorgenommen: a) der Absatz 2 des Unterabschnittes 9.1.1 wurde inhaltlich
DIN EN ISO 6888-3 DIN EN ISO 6888-3:2003-09 DIN EN ISO 6888-3:2005-07 geändert, um Missverständnisse bei Anwendung der Norm zu vermeiden. Gioletti Cantoni - Baird Parker Agar
ISO 7954, 1987 Internal methode ISO 7954:1987 ISO 21527-1:2008 This part of ISO 21527, together with ISO 21527-2, cancel and replace ISO 7698:1990, ISO 7954:1987 and ISO 13681:1995. (keine weiteren Infor according to ISO 6579
ISO 7954, 1987 Internal methode ISO 7954:1987 ISO 21527-1:2008 This part of ISO 21527, together with ISO 21527-2, cancel and replace ISO 7698:1990, ISO 7954:1987 and ISO 13681:1995. (keine weiteren Informationen zu Änderungen)
ISO 7932 Internal methode ISO 7932:2004 ISO 7932:2004 Aktuelle Version MYP
The main changes, compared to ISO 6579:2002, are the following.
— ISO 6785 has been incorporated in this document.
— Samples from the primary production stage have been added to the scope.
— Detection of Salmonella Typhi and Salmonella Paratyphi is described in Annex
D.
— Descriptions of preparations of initial suspensions have been removed and
references made to relevant parts of ISO 6887, whenever possible.
— The temperature range for incubation of non-selective media has been
extended from 37 °C ± 1 °C to 34 °C to 38 °C without further tolerance.
— For selective enrichment, there is a choice between using the broth or the
semi-solid agar of Rappaport Vassiliadis medium (RVS or MSRV) for food, animal
feed samples, and for environmental samples from the food production area.
— The inoculation of the isolation medium has become less prescriptive; the
objective is to obtain well-isolated colonies after incubation.
— For confirmation, it is acceptable to perform the tests on only one suspect
colony (instead of one suspect colony of each medium combination). If this isolate
tests negative for Salmonella, four more suspect isolates from different media
combinations shall be tested.
— It is permitted to perform the biochemical confirmation directly on a suspect,
well-isolated colony from the selective plating medium. The purity check on the
non-selective agar medium can then be performed in parallel.
— Two confirmation tests have become optional (ß-galactosidase test and indole
reaction) and one confirmation test has been deleted (Voges-Proskauer reaction).
— In this document, serological confirmation (to serogroup level) is described. For
guidance on serotyping (to serovar level), reference is made to ISO/TR 6579-3.
— Table 1 has been improved.
— Performance testing for the quality assurance of the culture media has been
added to Annex B.
EN 12824, 1997 Internal methode Barrel Methode - following the ISO 6579:2002 ISO 6579-1:2017 — Performance characteristics of MSRV have been added to Annex C. according to ISO 6579
ISO 7937:2004-08 ISO 7937:2004 ISO 7937:2004 Aktuelle Version RPM & Crossley Milk eventuell TSC
ISO 7899-2:2000 ISO 7899-2:2000 ISO 7899-2:2000 Aktuelle Version KAAB
ISO 11920-1:1996-12 ISO 11920-1:1996-12 ISO 11920-1:1996-12 Aktuelle Version Fraser broth sup- Palcam and Rapid L#mono
Überarbeitet 21.08.2018
ISO 29981|IDF 220:2010 specifies a method for the selective enumeration of
presumptive bifidobacteria in milk products by using a colony count technique at 37
°C under anaerobic conditions.
The method is applicable to milk products such as fermented and non-fermented
milks, milk powders, infant formulae, and starter cultures where these
microorganisms are present and viable, and in combination with other lactic acid
bacteria.
Bifidobacteria used in milk products usually belong to the species: Bifidobacterium
adolescentis; B. animalis subsp. animalis; B. animalis subsp. lactis; B. bifidum; B.
breve; B. infantis; B. longum.
ISO 29981|IDF 220:2010; Internal methode ISO 29981|IDF 220:2010 ISO 29981|IDF 220:2010
Überarbeitet 25.03.2020