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Student: ___________________________________________________________________________

1. The Five I's of studying microorganisms include all of the following except
A. Inoculation
B. Incubation
C. Infection
D. Isolation
E. Identification
2. The term that refers to the purposeful addition of microorganisms into a laboratory nutrient medium
is
A. Isolation
B. Inoculation
C. Immunization
D. Infection
E. Contamination
3. A pure culture contains
A. Only one species of microorganism
B. Only bacteria
C. A variety of microbes from one source
D. A variety of species from the same genus
E. None of the choices are correct
4. The correct microbiological term for the tiny sample of specimen that is put ino a nutrient medium in
order to produce a culture is the
A. Colony
B. Inoculum
C. Streak
D. Loop
E. None of the choices are correct
5. Which of the following is essential for development of discrete, isolated colonies?
A. Broth medium
B. Differential medium
C. Selective medium
D. Solid medium
E. Assay medium
6. Which method often results in colonies developing down throughout the agar along with some colonies
on the surface?
A. Streak plate
B. Spread plate
C. Pour plate
D. All of the choices are correct
E. None of the choices are correct
7. What type of isolation technique is most effective for the majority of applications?
A. Pour plate
B. Streak plate
C. Spread plate
D. Loop dilution
E. Culture plate
8. Which of the following will result when 1% to 5% agar is added to nutrient broth, boiled and cooled?
A. A pure culture
B. A mixed culture
C. A solid medium
D. A liquid medium
E. A contaminated medium
9. Agar is an important component of media because
A. Bacteria require agar to grow
B. Agar inhibits mold growth
C. Agar provides a solid surface for bacterial growth
D. Agar prevents contamination
E. All of the choices are correct
10. The three physical forms of laboratory media are
A. Solid, liquid, gas
B. Solid, semisolid, liquid
C. Streak plate, pour plate, broth
D. Aerobic, anaerobic, micro aerobic
E. None of the choices are correct
11. Which of the following is not an inoculating tool?
A. Petri dish
B. Loop
C. Needle
D. Pipette
E. Swab
12. Agar is a complex polysaccharide that comes from a/an
A. Green plant
B. Fungus
C. Mold
D. Algae
E. Euglena
13. Which of the following is not a benefit of agar as a solid medium?
A. Flexibility
B. Holds moisture
C. Can be inoculated and poured at a temperature that is not harmful
D. Solid at room temperature
E. Is digested by most microbes
14. A nutrient medium that has all of its chemical components identified and their precise concentrations
known and reproducible, would be termed
A. Complex
B. Reducing
C. Enriched
D. Synthetic
E. None of the choices are correct
15. A nutrient medium that contains at least one ingredient that is NOT chemically definable would be
termed
A. Complex
B. Reducing
C. Enriched
D. Synthetic
E. None of the choices are correct
16. All of the following are examples of different types of microbiological media except
A. Broth
B. Enriched
C. Agar
D. Petri dish
E. Gel
17. A microbiologist inoculates Staphylococcus epidermidis and Escherichia coli into a culture medium.
Following incubation, only the E. coli grows in the culture. What is the most likely explanation?
A. The microbiologist used too much inoculum
B. The culture is contaminated
C. The incubation temperature was incorrect
D. The culture medium must be selective
E. The culture medium must be differential
18. A common medium used for growing fastidious bacteria is
A. Blood agar
B. Trypticase soy agar
C. Mannitol salt agar
D. MacConkey medium
E. A reducing medium
19. A reducing medium contains
A. Sugars that can be fermented
B. Extra oxygen
C. Hemoglobin, vitamins or other growth factors
D. Substances that remove oxygen
E. Inhibiting agents
20. Which type of medium is able to distinguish different species or types of microorganisms based on an
observable change in the colonies or in the medium?
A. Differential
B. Selective
C. Enumeration
D. Enriched
E. Reducing
21. A microbiologist decides to use a nutrient medium that contains thioglycollic acid. What type of microbe
is she attempting to culture?
A. Fastidious
B. Gram positive
C. Anaerobic
D. Gram negative
E. Aerobic
22. Differential media results in which of the following growth characteristics?
A. Different color colonies
B. Different media color post incubation
C. Precipitates
D. Gas bubbles
E. All of the choices are correct
23. A reducing media is used to culture
A. Fastidious organisms
B. Aerobic organisms
C. Anaerobic organisms
D. Any pathogenic organisms
E. None of the choices are correct
24. For which bacterial genus is mannitol salt agar selective?
A. Salmonella
B. Streptococcus
C. Neisseria
D. Staphylococcus
E. Escherichia
25. A microbiologist must culture a patient's feces for intestinal pathogens. Which of the following would
likely be present in selective media for analyzing this fecal specimen?
A. NaCl
B. Sheep red blood cells
C. Bile salts
D. Thioglycollic acid
E. Peptone
26. Bacteria that require special growth factors and complex nutrients are termed
A. Aerobic
B. Anaerobic
C. Fastidious
D. Microaerophilic
E. Autotrophic
27. A microbiologist inoculates Staphylococcus aureus into a culture medium. Following incubation, both
Staphylococcus aureus and Staphylococcus epidermidis are determined to be growing in this culture.
What is the most likely explanation?
A. The microbiologist used too much inoculum
B. The culture is contaminated
C. The incubation temperature was incorrect
D. The culture medium must be selective
E. The culture medium must be differential
28. What is the term for a culture made from one isolated colony?
A. Axenic
B. Mixed
C. Pure
D. Both axenic and mixed
E. Both axenic and pure
29. Newly inoculated cultures must be _____ at a specific temperature and time to encourage growth.
A. Streaked
B. Poured
C. Incubated
D. All of the choices are correct
30. The _____ of the microscope holds and allows selection of the objective lenses.
A. Stage
B. Condenser
C. Objective
D. Ocular
E. Nosepiece
31. Which of the following will converge light rays to a single focal point on the specimen?
A. Ocular lens
B. Objective lens
C. Iris diaphragm
D. Condenser
E. Nosepiece
32. Which of the following magnifies the specimen to produce the real image of the specimen?
A. Condenser
B. Objective lens
C. Ocular lens
D. Body
E. Nosepiece
33. Which of the following magnifies the specimen to produce the virtual image of the specimen?
A. Objective lens
B. Ocular lens
C. Condenser
D. Body
E. Iris diaphragm
34. Which of the following controls the amount of light entering the specimen?
A. Objective lens
B. Ocular lens
C. Condenser
D. Body
E. Iris diaphragm
35. If a microbiologist is studying a specimen at a total magnification of 950X, what is the magnifying power
of the objective lens if the ocular lens is 10X?
A. 100X
B. 950X
C. 85X
D. 850X
E. 95X
36. Magnification is achieved in a compound microscope through the initial magnification of the specimen
by the _____ lens. This image is then projected to the _____ lens that will further magnify the specimen
to form a virtual image received by the eye.
A. Ocular, objective
B. Scanning, objective
C. Objective, ocular
D. Ocular, oil
E. None of the choices are correct
37. Which of the following characteristics refers to the microscope's ability to show two separate entities as
separate and distinct?
A. Resolving power
B. Magnification
C. Refraction
D. All of the choices are correct
E. None of the choices are correct
38. All of the following are diameters of cells that would be resolved in a microscope with a limit of
resolution of 0.2µm except
A. 0.2 µm
B. 0.2 mm
C. 0.1 µm
D. 0.3 µm
E. 2.0 µm
39. The wavelength of light used and the numerical aperture governs
A. Illumination
B. Resolution
C. Magnification
D. Size of the field
E. All of the choices are correct
40. _____ has the same optical qualities as glass and thus prevents refractive loss of light as it passes from the
slide to the objective lens.
A. The scanning objective
B. The oil objective
C. The slide
D. Immersion oil
E. The ocular
41. The type of microscope in which you would see brightly illuminated specimens against a black
background is
A. Bright-field
B. Dark-field
C. Phase-contrast
D. Fluorescence
E. Electron
42. Which type of microscope shows cells against a bright background and also shows intracellular structures
of unstained cells based on their varying densities?
A. Bright-field
B. Dark-field
C. Phase-contrast
D. Differential interference
E. Electron
43. Which type of microscope is the most widely used and shows cells against a bright background?
A. Bright-field
B. Dark-field
C. Phase-contrast
D. Fluorescence
E. Electron
44. All of the following pertain to the fluorescence microscope except
A. Uses electrons to produce a specimen image
B. Type of compound microscope
C. Requires the use of dyes like acridine and fluoresce in
D. Commonly used to diagnose certain infections
E. Requires an ultraviolet radiation source
45. A confocal scanning optical microscope
A. Uses ultraviolet light to form a specimen image
B. Shows three-dimensional cell images from the cell surface to the middle of the cell
C. Produces specimen images on electron micrographs
D. Uses dyes that emit visible light when bombarded by ultraviolet rays
E. Requires specimens to be stained
46. A confocal scanning optical microscope
A. Uses a laser beam of light to form a specimen image
B. Shows only the surface of the specimen
C. Produces specimen images on electron micrographs
D. Uses dyes that emit visible light when bombarded by ultraviolet rays
E. Requires specimens to be stained
47. Which type of microscope does not use light in forming the specimen image?
A. Bright-field
B. Dark-field
C. Phase-contrast
D. Fluorescence
E. Electron
48. Which type of microscope achieves the greatest resolution and highest magnification?
A. Bright-field
B. Dark-field
C. Phase-contrast
D. Fluorescence
E. Electron
49. Which type of microscope cannot image live specimens?
A. Bright-field
B. Phase-contrast
C. Dark-field
D. Tunneling electron
E. Differential interference
50. Which type of microscope bombards a whole, metal-coated specimen with electrons moving back and
forth over it?
A. Fluorescence
B. Differential interference contrast
C. Scanning electron
D. Transmission electron
E. Phase-contrast
51. The specimen preparation that is best for viewing cell motility is
A. Hanging drop
B. Fixed stained smear
C. Gram stain
D. Negative stain
E. Flagellar stain
52. The purpose of staining cells on a microscope slide is to
A. Kill them
B. Secure them to the slide
C. Enlarge the cells
D. Add contrast in order to see them better
E. See motility
53. The gram staining procedure is best described as a __staining technique.
A. acid fast or Ziehl-Neelson
B. differential
C. capsule
D. Schaefer-Fulton
54. What do the Gram stain, acid-fast stain and endospore stain have in common?
A. Used on a wet mount of the specimen
B. Use heat to force the dye into cell structures
C. Outcome based on cell wall differences
D. Use a negative stain technique
E. Are differential stains
55. Basic dyes are
A. Attracted to the acidic substances of bacterial cells
B. Anionic
C. Used in negative staining
D. Repelled by cells
E. Dyes such as India ink and nigrosin
56. A microbiologist makes a fixed smear of bacterial cells and stains them with Loeffler's methylene blue.
All the cells appear blue under the oil lens. This is an example of
A. Negative staining
B. Using an acidic dye
C. Simple staining
D. Using the acid-fast stain
E. Capsule staining
57. Matching.
1.) 1 Km A.) 10-2 cm
2.) 1 mm B.) 100 mm
3.) 10 cm C.) 10-6 m
4.) 1 m D.) 1000 m
Ans:1. D 2. A 3. B 4. C
58. Who invented the first crude microscope by grinding glass?
A. Redi
B. Lister
C. Schultz & Schwann
D. Leeuwenhoek
59. Which type of media would be the best choice when shipping a sample of bacteria to a laboratory to be
tested from a satellite office site.
A. Transport
B. EMB
C. Blood
D. thioglycollate
60. The Gram staining procedure is best described as a __staining technique.
A. Acid fast or Ziehl-Neelson
B. Differential
C. Capsule
D. Schaefer-Fulton
61. The procedures for culturing a microorganism require the use of a microscope.
True False
62. A medium that is gel-like has less agar in it compared to a solid medium.
True False
63. Some microbes are not capable of growing on artificial media.
True False
64. A selective medium contains one or more substances that inhibit growth of certain microbes in order to
facilitate the growth of other microbes.
True False
65. One colony typically develops from the growth of several parent bacterial cells.
True False
66. Mixed cultures are also referred to as contaminated cultures.
True False
67. Bacterial cultures are easily identified from their microscopic appearance.
True False
68. Normal incubation temperatures range from 30° to 60° C.
True False
69. The bending of light rays as they pass form one medium to another is called refraction.
True False
70. The real image is the reverse of the actual specimen.
True False
71. A differential interference contrast microscope uses dyes to give colored three-dimensional images.
True False
72. Fixed smears of specimens are required in order to perform the Gram stain and endospore stain on the
specimens.
True False
73. At the end of the Gram stain, gram positive bacteria will be seen as pink cells.
True False
3 Key
1. The Five I's of studying microorganisms include all of the following except
A. Inoculation
B. Incubation
C. Infection
D. Isolation
E. Identification
Cowan - Chapter 03 #1
Learning Objective: 3.01 Explain what the 5 Is mean and what each step entails.
2. The term that refers to the purposeful addition of microorganisms into a laboratory nutrient medium
is
A. Isolation
B. Inoculation
C. Immunization
D. Infection
E. Contamination
Cowan - Chapter 03 #2
Learning Objective: 3.01 Explain what the 5 Is mean and what each step entails.
3. A pure culture contains
A. Only one species of microorganism
B. Only bacteria
C. A variety of microbes from one source
D. A variety of species from the same genus
E. None of the choices are correct
Cowan - Chapter 03 #3
Learning Objective: 3.01 Explain what the 5 Is mean and what each step entails.
4. The correct microbiological term for the tiny sample of specimen that is put ino a nutrient medium in
order to produce a culture is the
A. Colony
B. Inoculum
C. Streak
D. Loop
E. None of the choices are correct
Cowan - Chapter 03 #4
Learning Objective: 3.01 Explain what the 5 Is mean and what each step entails.
5. Which of the following is essential for development of discrete, isolated colonies?
A. Broth medium
B. Differential medium
C. Selective medium
D. Solid medium
E. Assay medium
Cowan - Chapter 03 #5
Learning Objective: 3.02 Name and define the 3 ways to categorize media.
6. Which method often results in colonies developing down throughout the agar along with some
colonies on the surface?
A. Streak plate
B. Spread plate
C. Pour plate
D. All of the choices are correct
E. None of the choices are correct
Cowan - Chapter 03 #6
Learning Objective: 3.03 Provide examples for each of the 3 categories of media.
7. What type of isolation technique is most effective for the majority of applications?
A. Pour plate
B. Streak plate
C. Spread plate
D. Loop dilution
E. Culture plate
Cowan - Chapter 03 #7
Learning Objective: 3.01 Explain what the 5 Is mean and what each step entails.
8. Which of the following will result when 1% to 5% agar is added to nutrient broth, boiled and cooled?

A. A pure culture
B. A mixed culture
C. A solid medium
D. A liquid medium
E. A contaminated medium
Cowan - Chapter 03 #8
Learning Objective: 3.01 Explain what the 5 Is mean and what each step entails.
9. Agar is an important component of media because
A. Bacteria require agar to grow
B. Agar inhibits mold growth
C. Agar provides a solid surface for bacterial growth
D. Agar prevents contamination
E. All of the choices are correct
Cowan - Chapter 03 #9
Learning Objective: 3.01 Explain what the 5 Is mean and what each step entails.
10. The three physical forms of laboratory media are
A. Solid, liquid, gas
B. Solid, semisolid, liquid
C. Streak plate, pour plate, broth
D. Aerobic, anaerobic, micro aerobic
E. None of the choices are correct
Cowan - Chapter 03 #10
Learning Objective: 3.02 Name and define the 3 ways to categorize media.
11. Which of the following is not an inoculating tool?
A. Petri dish
B. Loop
C. Needle
D. Pipette
E. Swab
Cowan - Chapter 03 #11
Learning Objective: 3.01 Explain what the 5 Is mean and what each step entails.
12. Agar is a complex polysaccharide that comes from a/an
A. Green plant
B. Fungus
C. Mold
D. Algae
E. Euglena
Cowan - Chapter 03 #12
Learning Objective: 3.02 Name and define the 3 ways to categorize media.
13. Which of the following is not a benefit of agar as a solid medium?
A. Flexibility
B. Holds moisture
C. Can be inoculated and poured at a temperature that is not harmful
D. Solid at room temperature
E. Is digested by most microbes
Cowan - Chapter 03 #13
Learning Objective: 3.02 Name and define the 3 ways to categorize media.
14. A nutrient medium that has all of its chemical components identified and their precise concentrations
known and reproducible, would be termed
A. Complex
B. Reducing
C. Enriched
D. Synthetic
E. None of the choices are correct
Cowan - Chapter 03 #14
Learning Objective: 3.03 Provide examples for each of the 3 categories of media.
15. A nutrient medium that contains at least one ingredient that is NOT chemically definable would be
termed
A. Complex
B. Reducing
C. Enriched
D. Synthetic
E. None of the choices are correct
Cowan - Chapter 03 #15
Learning Objective: 3.03 Provide examples for each of the 3 categories of media.
16. All of the following are examples of different types of microbiological media except
A. Broth
B. Enriched
C. Agar
D. Petri dish
E. Gel
Cowan - Chapter 03 #16
Learning Objective: 3.03 Provide examples for each of the 3 categories of media.
17. A microbiologist inoculates Staphylococcus epidermidis and Escherichia coli into a culture medium.
Following incubation, only the E. coli grows in the culture. What is the most likely explanation?
A. The microbiologist used too much inoculum
B. The culture is contaminated
C. The incubation temperature was incorrect
D. The culture medium must be selective
E. The culture medium must be differential
Cowan - Chapter 03 #17
Learning Objective: 3.01 Explain what the 5 Is mean and what each step entails.
18. A common medium used for growing fastidious bacteria is
A. Blood agar
B. Trypticase soy agar
C. Mannitol salt agar
D. MacConkey medium
E. A reducing medium
Cowan - Chapter 03 #18
Learning Objective: 3.03 Provide examples for each of the 3 categories of media.
19. A reducing medium contains
A. Sugars that can be fermented
B. Extra oxygen
C. Hemoglobin, vitamins or other growth factors
D. Substances that remove oxygen
E. Inhibiting agents
Cowan - Chapter 03 #19
Learning Objective: 3.03 Provide examples for each of the 3 categories of media.
20. Which type of medium is able to distinguish different species or types of microorganisms based on an
observable change in the colonies or in the medium?
A. Differential
B. Selective
C. Enumeration
D. Enriched
E. Reducing
Cowan - Chapter 03 #20
Learning Objective: 3.03 Provide examples for each of the 3 categories of media.
21. A microbiologist decides to use a nutrient medium that contains thioglycollic acid. What type of
microbe is she attempting to culture?
A. Fastidious
B. Gram positive
C. Anaerobic
D. Gram negative
E. Aerobic
Cowan - Chapter 03 #21
Learning Objective: 3.03 Provide examples for each of the 3 categories of media.
22. Differential media results in which of the following growth characteristics?
A. Different color colonies
B. Different media color post incubation
C. Precipitates
D. Gas bubbles
E. All of the choices are correct
Cowan - Chapter 03 #22
Learning Objective: 3.03 Provide examples for each of the 3 categories of media.
23. A reducing media is used to culture
A. Fastidious organisms
B. Aerobic organisms
C. Anaerobic organisms
D. Any pathogenic organisms
E. None of the choices are correct
Cowan - Chapter 03 #23
Learning Objective: 3.03 Provide examples for each of the 3 categories of media.
24. For which bacterial genus is mannitol salt agar selective?
A. Salmonella
B. Streptococcus
C. Neisseria
D. Staphylococcus
E. Escherichia
Cowan - Chapter 03 #24
Learning Objective: 3.03 Provide examples for each of the 3 categories of media.
25. A microbiologist must culture a patient's feces for intestinal pathogens. Which of the following would
likely be present in selective media for analyzing this fecal specimen?
A. NaCl
B. Sheep red blood cells
C. Bile salts
D. Thioglycollic acid
E. Peptone
Cowan - Chapter 03 #25
Learning Objective: 3.03 Provide examples for each of the 3 categories of media.
26. Bacteria that require special growth factors and complex nutrients are termed
A. Aerobic
B. Anaerobic
C. Fastidious
D. Microaerophilic
E. Autotrophic
Cowan - Chapter 03 #26
Learning Objective: 3.03 Provide examples for each of the 3 categories of media.
27. A microbiologist inoculates Staphylococcus aureus into a culture medium. Following incubation, both
Staphylococcus aureus and Staphylococcus epidermidis are determined to be growing in this culture.
What is the most likely explanation?
A. The microbiologist used too much inoculum
B. The culture is contaminated
C. The incubation temperature was incorrect
D. The culture medium must be selective
E. The culture medium must be differential
Cowan - Chapter 03 #27
Learning Objective: 3.03 Provide examples for each of the 3 categories of media.
28. What is the term for a culture made from one isolated colony?
A. Axenic
B. Mixed
C. Pure
D. Both axenic and mixed
E. Both axenic and pure
Cowan - Chapter 03 #28
Learning Objective: 3.01 Explain what the 5 Is mean and what each step entails.
29. Newly inoculated cultures must be _____ at a specific temperature and time to encourage growth.
A. Streaked
B. Poured
C. Incubated
D. All of the choices are correct
Cowan - Chapter 03 #29
Learning Objective: 3.01 Explain what the 5 Is mean and what each step entails.
30. The _____ of the microscope holds and allows selection of the objective lenses.
A. Stage
B. Condenser
C. Objective
D. Ocular
E. Nosepiece
Cowan - Chapter 03 #30
Learning Objective: 3.05 Describe the earliest microscopes.
Learning Objective: 3.07 Differentiate between the principles of light and electron microscopy.
31. Which of the following will converge light rays to a single focal point on the specimen?
A. Ocular lens
B. Objective lens
C. Iris diaphragm
D. Condenser
E. Nosepiece
Cowan - Chapter 03 #31
Learning Objective: 3.07 Differentiate between the principles of light and electron microscopy.
32. Which of the following magnifies the specimen to produce the real image of the specimen?
A. Condenser
B. Objective lens
C. Ocular lens
D. Body
E. Nosepiece
Cowan - Chapter 03 #32
Learning Objective: 3.07 Differentiate between the principles of light and electron microscopy.
33. Which of the following magnifies the specimen to produce the virtual image of the specimen?
A. Objective lens
B. Ocular lens
C. Condenser
D. Body
E. Iris diaphragm
Cowan - Chapter 03 #33
Learning Objective: 3.07 Differentiate between the principles of light and electron microscopy.
34. Which of the following controls the amount of light entering the specimen?
A. Objective lens
B. Ocular lens
C. Condenser
D. Body
E. Iris diaphragm
Cowan - Chapter 03 #34
Learning Objective: 3.07 Differentiate between the principles of light and electron microscopy.
35. If a microbiologist is studying a specimen at a total magnification of 950X, what is the magnifying
power of the objective lens if the ocular lens is 10X?
A. 100X
B. 950X
C. 85X
D. 850X
E. 95X
Cowan - Chapter 03 #35
Learning Objective: 3.07 Differentiate between the principles of light and electron microscopy.
36. Magnification is achieved in a compound microscope through the initial magnification of the
specimen by the _____ lens. This image is then projected to the _____ lens that will further magnify
the specimen to form a virtual image received by the eye.
A. Ocular, objective
B. Scanning, objective
C. Objective, ocular
D. Ocular, oil
E. None of the choices are correct
Cowan - Chapter 03 #36
Learning Objective: 3.07 Differentiate between the principles of light and electron microscopy.
37. Which of the following characteristics refers to the microscope's ability to show two separate entities
as separate and distinct?
A. Resolving power
B. Magnification
C. Refraction
D. All of the choices are correct
E. None of the choices are correct
Cowan - Chapter 03 #37
Learning Objective: 3.07 Differentiate between the principles of light and electron microscopy.
38. All of the following are diameters of cells that would be resolved in a microscope with a limit of
resolution of 0.2µm except
A. 0.2 µm
B. 0.2 mm
C. 0.1 µm
D. 0.3 µm
E. 2.0 µm
Cowan - Chapter 03 #38
Learning Objective: 3.04 Convert among different lengths within the metric system.
Learning Objective: 3.07 Differentiate between the principles of light and electron microscopy.
39. The wavelength of light used and the numerical aperture governs
A. Illumination
B. Resolution
C. Magnification
D. Size of the field
E. All of the choices are correct
Cowan - Chapter 03 #39
Learning Objective: 3.07 Differentiate between the principles of light and electron microscopy.
40. _____ has the same optical qualities as glass and thus prevents refractive loss of light as it passes from
the slide to the objective lens.
A. The scanning objective
B. The oil objective
C. The slide
D. Immersion oil
E. The ocular
Cowan - Chapter 03 #40
Learning Objective: 3.07 Differentiate between the principles of light and electron microscopy.
41. The type of microscope in which you would see brightly illuminated specimens against a black
background is
A. Bright-field
B. Dark-field
C. Phase-contrast
D. Fluorescence
E. Electron
Cowan - Chapter 03 #41
Learning Objective: 3.07 Differentiate between the principles of light and electron microscopy.
42. Which type of microscope shows cells against a bright background and also shows intracellular
structures of unstained cells based on their varying densities?
A. Bright-field
B. Dark-field
C. Phase-contrast
D. Differential interference
E. Electron
Cowan - Chapter 03 #42
Learning Objective: 3.07 Differentiate between the principles of light and electron microscopy.
43. Which type of microscope is the most widely used and shows cells against a bright background?
A. Bright-field
B. Dark-field
C. Phase-contrast
D. Fluorescence
E. Electron
Cowan - Chapter 03 #43
Learning Objective: 3.07 Differentiate between the principles of light and electron microscopy.
44. All of the following pertain to the fluorescence microscope except
A. Uses electrons to produce a specimen image
B. Type of compound microscope
C. Requires the use of dyes like acridine and fluoresce in
D. Commonly used to diagnose certain infections
E. Requires an ultraviolet radiation source
Cowan - Chapter 03 #44
Learning Objective: 3.07 Differentiate between the principles of light and electron microscopy.
45. A confocal scanning optical microscope
A. Uses ultraviolet light to form a specimen image
B. Shows three-dimensional cell images from the cell surface to the middle of the cell
C. Produces specimen images on electron micrographs
D. Uses dyes that emit visible light when bombarded by ultraviolet rays
E. Requires specimens to be stained
Cowan - Chapter 03 #45
Learning Objective: 3.07 Differentiate between the principles of light and electron microscopy.
46. A confocal scanning optical microscope
A. Uses a laser beam of light to form a specimen image
B. Shows only the surface of the specimen
C. Produces specimen images on electron micrographs
D. Uses dyes that emit visible light when bombarded by ultraviolet rays
E. Requires specimens to be stained
Cowan - Chapter 03 #46
Learning Objective: 3.07 Differentiate between the principles of light and electron microscopy.
47. Which type of microscope does not use light in forming the specimen image?
A. Bright-field
B. Dark-field
C. Phase-contrast
D. Fluorescence
E. Electron
Cowan - Chapter 03 #47
Learning Objective: 3.07 Differentiate between the principles of light and electron microscopy.
48. Which type of microscope achieves the greatest resolution and highest magnification?
A. Bright-field
B. Dark-field
C. Phase-contrast
D. Fluorescence
E. Electron
Cowan - Chapter 03 #48
Learning Objective: 3.06 List and describe the 3 elements of good microscopy.
Learning Objective: 3.07 Differentiate between the principles of light and electron microscopy.
49. Which type of microscope cannot image live specimens?
A. Bright-field
B. Phase-contrast
C. Dark-field
D. Tunneling electron
E. Differential interference
Cowan - Chapter 03 #49
Learning Objective: 3.07 Differentiate between the principles of light and electron microscopy.
50. Which type of microscope bombards a whole, metal-coated specimen with electrons moving back and
forth over it?
A. Fluorescence
B. Differential interference contrast
C. Scanning electron
D. Transmission electron
E. Phase-contrast
Cowan - Chapter 03 #50
Learning Objective: 3.07 Differentiate between the principles of light and electron microscopy.
51. The specimen preparation that is best for viewing cell motility is
A. Hanging drop
B. Fixed stained smear
C. Gram stain
D. Negative stain
E. Flagellar stain
Cowan - Chapter 03 #51
Learning Objective: 3.07 Differentiate between the principles of light and electron microscopy.
52. The purpose of staining cells on a microscope slide is to
A. Kill them
B. Secure them to the slide
C. Enlarge the cells
D. Add contrast in order to see them better
E. See motility
Cowan - Chapter 03 #52
Learning Objective: 3.06 List and describe the 3 elements of good microscopy.
Learning Objective: 3.08 Name the 2 main categories of stains.
53. The gram staining procedure is best described as a __staining technique.
A. acid fast or Ziehl-Neelson
B. differential
C. capsule
D. Schaefer-Fulton
Cowan - Chapter 03 #53
54. What do the Gram stain, acid-fast stain and endospore stain have in common?
A. Used on a wet mount of the specimen
B. Use heat to force the dye into cell structures
C. Outcome based on cell wall differences
D. Use a negative stain technique
E. Are differential stains
Cowan - Chapter 03 #54
Learning Objective: 3.09 Give examples of simple
Learning Objective: and special stain.
Learning Objective: differential
55. Basic dyes are
A. Attracted to the acidic substances of bacterial cells
B. Anionic
C. Used in negative staining
D. Repelled by cells
E. Dyes such as India ink and nigrosin
Cowan - Chapter 03 #55
Learning Objective: 3.09 Give examples of simple
Learning Objective: and special stain.
Learning Objective: differential
56. A microbiologist makes a fixed smear of bacterial cells and stains them with Loeffler's methylene
blue. All the cells appear blue under the oil lens. This is an example of
A. Negative staining
B. Using an acidic dye
C. Simple staining
D. Using the acid-fast stain
E. Capsule staining
Cowan - Chapter 03 #56
Learning Objective: 3.09 Give examples of simple
Learning Objective: and special stain.
Learning Objective: differential
57. Matching.
1.) 1 Km A.) 10-2 cm
2.) 1 mm B.) 100 mm
3.) 10 cm C.) 10-6 m
4.) 1 m D.) 1000 m
Ans:1. D 2. A 3. B 4. C
Cowan - Chapter 03 #57
Learning Objective: 3.04 Convert among different lengths within the metric system.
58. Who invented the first crude microscope by grinding glass?
A. Redi
B. Lister
C. Schultz & Schwann
D. Leeuwenhoek
Cowan - Chapter 03 #58
Learning Objective: 3.05 Describe the earliest microscopes.
59. Which type of media would be the best choice when shipping a sample of bacteria to a laboratory to
be tested from a satellite office site.
A. Transport
B. EMB
C. Blood
D. thioglycollate
Cowan - Chapter 03 #59
Learning Objective: 3.03 Provide examples for each of the 3 categories of media.
60. The Gram staining procedure is best described as a __staining technique.
A. Acid fast or Ziehl-Neelson
B. Differential
C. Capsule
D. Schaefer-Fulton
Cowan - Chapter 03 #60
Learning Objective: 3.08 Name the 2 main categories of stains.
Learning Objective: 3.09 Give examples of a simple
Learning Objective: and special stain.
Learning Objective: differential
61. The procedures for culturing a microorganism require the use of a microscope.
FALSE
Cowan - Chapter 03 #61
Learning Objective: 3.01 Explain what the 5 Is mean and what each step entails.
62. A medium that is gel-like has less agar in it compared to a solid medium.
TRUE
Cowan - Chapter 03 #62
Learning Objective: 3.02 Name & define the 3 ways to categorize media.
63. Some microbes are not capable of growing on artificial media.
TRUE
Cowan - Chapter 03 #63
Learning Objective: 3.01 Explain what the 5 Is mean and what each step entails.
64. A selective medium contains one or more substances that inhibit growth of certain microbes in order
to facilitate the growth of other microbes.
TRUE
Cowan - Chapter 03 #64
Learning Objective: 3.03 Provide examples for each of the 3 categories of media.
65. One colony typically develops from the growth of several parent bacterial cells.
FALSE
Cowan - Chapter 03 #65
Learning Objective: 3.01 Explain what the 5 Is mean and what each step entails.
66. Mixed cultures are also referred to as contaminated cultures.
FALSE
Cowan - Chapter 03 #66
Learning Objective: 3.01 Explain what the 5 Is mean and what each step entails.
67. Bacterial cultures are easily identified from their microscopic appearance.
FALSE
Cowan - Chapter 03 #67
Learning Objective: 3.01 Explain what the 5 Is mean and what each step entails.
68. Normal incubation temperatures range from 30° to 60° C.
FALSE
Cowan - Chapter 03 #68
Learning Objective: 3.01 Explain what the 5 Is mean and what each step entails.
69. The bending of light rays as they pass form one medium to another is called refraction.
TRUE
Cowan - Chapter 03 #69
Learning Objective: 3.07 Differentiate between the principles of light and electron microscopy.
70. The real image is the reverse of the actual specimen.
FALSE
Cowan - Chapter 03 #70
Learning Objective: 3.07 Differentiate between the principles of light and electron microscopy.
71. A differential interference contrast microscope uses dyes to give colored three-dimensional
images.
FALSE
Cowan - Chapter 03 #71
Learning Objective: 3.06 List and describe the 3 elements of good microscopy.
Learning Objective: 3.07 Differentiate between the principles of light and electron microscopy.
72. Fixed smears of specimens are required in order to perform the Gram stain and endospore stain on the
specimens.
TRUE
Cowan - Chapter 03 #72
Learning Objective: 3.06 List and describe the 3 elements of good microscopy.
Learning Objective: 3.09 Give examples of simple
Learning Objective: and special stain.
Learning Objective: differential
73. At the end of the Gram stain, gram positive bacteria will be seen as pink cells.
FALSE
Cowan - Chapter 03 #73
Learning Objective: 3.06 List & describe the 3 elements of good microscopy.
Learning Objective: 3.09 Give examples of simple
Learning Objective: and special stain.
Learning Objective: differential
3 Summary
Category # of Questions
Cowan - Chapter 03 73
Learning Objective: 3.01 Explain what the 5 Is mean and what each step entails. 17
Learning Objective: 3.02 Name & define the 3 ways to categorize media. 1
Learning Objective: 3.02 Name and define the 3 ways to categorize media. 4
Learning Objective: 3.03 Provide examples for each of the 3 categories of media. 16
Learning Objective: 3.04 Convert among different lengths within the metric system. 2
Learning Objective: 3.05 Describe the earliest microscopes. 2
Learning Objective: 3.06 List & describe the 3 elements of good microscopy. 1
Learning Objective: 3.06 List and describe the 3 elements of good microscopy. 4
Learning Objective: 3.07 Differentiate between the principles of light and electron microscopy. 25
Learning Objective: 3.08 Name the 2 main categories of stains. 2
Learning Objective: 3.09 Give examples of a simple 1
Learning Objective: 3.09 Give examples of simple 5
Learning Objective: and special stain. 6
Learning Objective: differential 6

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