Instruction: Each of the questions or incomplete statements below is followed by four

suggested answers or completions. Select the one that is best in case and write the letter of
your best choice on the appropriate space provided.
1. Suppose one of the Science remedial students of Bahir Dar University would like to see the
internal body structures like kidneys and heart of a frog. Which one of the following tools of
biologists is appropriate?
A. Mass
B. Optical microscope
C. Dissecting kit
D. Petri dishes
What is the effect of intermittent fasting on blood sugar level of hypertension patients?
2. From the above statement, the independent variable is___________
A. Hypertension
B. Intermittent Fasting
C. Blood sugar level
D. Effect
3. The spontaneous generation theory states that “living things are arise from non-living things
spontaneously.” This theory was proposed by:
A. Lazzaro Spallanzini
B. Francesco Redii
C. Jhon Needham
D. Arstotile
4. Assume that, you did an experiment which has been done by somebody else so far and you got
more or less the same results? This tells us _________ of an experiment.
A. Validity
B. Reliability
C. Accuracy
D. Dependability
5. Which properties of water are important to control the core temperature of the body in human
beings?
A. Water is transparent
B. Water has high specific heat capacity
C. Water is transport medium
D. Water has high latent heat of vaporization
6. Most of the chemical reactions in the cell use water as ideal medium and takes place in__
A. Cell membrane
B. Cytosol of cytoplasm
C. Inside organelles
D. None
7. Which part of laboratory/experiment report states clearly what is being investigated?
A. Title
B. Acknowledgment
C. Result
 D. Materials used and Methods
8. Assume that one of your classmates did an experiment on unknown food sample using test
tube. After the experiment the student observed brick red precipitate in a test tube. This
test is called ____
A. Iodine Test
B. Benedict’s test
C. Biuret test
D. Grease spot test
9. Which one of the following pairs of carbohydrates are isomers?
A. Ribulose & dihydroxyacetate
B. Glucose & Ribose
C. Glyceraldehyde & fructose
D. Ribose & Ribulose
10. The correct structural formula of lactose is:

A) B) C) D) None
11. Which one of the following is not the function of triglycerides?
A. Structural component of cell membrane
B. Water proofing and buoyancy
C. Thermal insulation
D. None of these
12. Which one of the following is N- containing polysaccharide carbohydrate?
A. Starch
B. Glycogen
C. Cellulose
D. Chitin
13. The branch of science that study about the organic world of life is known as _____
A. Physical Science
B. Biological sciences
C. Experimental science
D. Applied Science
14. In an experiment, the variable that the experimenter cannot control and may influence the
result of the experiment is known as____________
A. Confounding variable
B. Independent Variable
C. Dependent Variable
D. Control variable
15. When a hypothesis is tested by repeated experiments and is not proved wrong, then it
becomes ________
A. Scientific law
B. Theory
C. Principle
D. All
E. None
16. A controlled experiment is one in which
A. There is one group in which the scientist controls all variables
 B. There are at least two groups, one differing from the other by two or more variables
C. There are at least two groups, one of which does not receive the experimental treatment
D. The experiment proceeds at a slow pace to guarantee that the scientist can carefully
observe all reactions and process all experimental data
17. Cellulose, the most important constituent of plant cell wall, is made up of
A. A branched chain of glucose molecules linked by β-1,4 glycosidic bond in straight chain
and α-1,6 glycosidic bond at the site of branching
B. Unbranched chain of glucose molecules linked by β-1,4 glycosidic bond
C. A branched chain of glucose molecules linked by β-1,4 glycosidic bond
D. Unbranched chain of glucose molecules linked by β-1,4 glycosidic bond in straight chain
and α-1,6 glycosidic bond at the site of branching
18. Assuming they all had the same number of carbon atoms, which of the following has the
most C-H bonds?
A. An unsaturated fat
B. A polyunsaturated fat
C. A saturated fat
D. A and B
19. The helical structure of proteins is stabilized by
A. Hydrogen bond
B. Disulphide bond
C. Peptide bond
D. None
20. Which particular nitrogenous base is present in DNA but not in RNA?
A. Adenine
B. Thymine
C. Uracil
D. Cytosine
21. A scientist testing the effects of a chemical on apple yield sprays an orchard with the
chemical. A second orchard does not receive the chemical. In the fall, the number of apples
harvested from each forest is counted. Which of the following is the independent (manipulated)
variable in the experiment?
A. The chemical
B. The number of apples
C. The first orchard
D. The second orchard
22. A hypothesis must be;
A. Proven correctly
B. Testable
C. Observed
D. Experimental
23. How many molecules of glycerol and fatty acids respectively are needed to form 100
molecules of phospholipid fat?
A. 100 &300
B. 100 &200
C. 150 &400
D. 100&100
24. Which of the following is not a part of the nucleotides that make up DNA?
A. Nitrogenous base
B. Sugar unit
C. Amino group
D. A phosphate group
25. What are the four major categories of organic compounds?
A. Acids, bases, nucleic acids, and polysaccharides
B. Carbohydrates, lipids, nucleic acids, and proteins
C. Carbon, hydrogen, nitrogen, and oxygen
D. Carbon, hydrogen, sulfur, and nitrogen
26. Which of the following corresponding to the first step of scientific methods
A. Performing an experiment
B. Formulate the hypothesis
C. Make an observation
D. Identifying the problem
E. Undertake the research
F. State the result
27. In an experiment that is testing the effect of light on the germination rate of certain seed,
which one of the following should the experimental and control group differ.
A. The amount of water they receive
B. The number of seed assigned to them
C. The age of the seed assigned to them
D. The amount of light they receive
28. The ability of microscope that used to distinguishes between two points that are close
together is referred to as:
A. Mounting
B. Magnification
C. Resolution
D. Staining
29. The appropriate tools of biologist which is more stable for cultivating microorganisms in the
lab are
A. Centrifuge
B. Beaker
C. Petri dish
D. Test tube

30. The major area of relevance and application of biology which is related to production of
transgenic organisms is:
A. Medicine
B. Agriculture
C. Biotechnology
D. Environment
31. The technique of growing plants from few cells on a special agar in the lab are referred to as
A. Selective breeding
B. Genetic engineering
C. Micro propagation
D. Cross breeding
32. Which of the following is the modern application of biotechnology?
 A. Production of dairy product by lactic acid fermentation
B. Alcoholic fermentation
C. Selective breeding
D. Genetic engineering
33. The properties of water that allow plants and algae in water to photosynthesize is
A. Surface tension
B. High heat of vaporization
C. Transparent
D. As universal solvent
34. What is the biological advantage of water in having high specific heat capacity?
A. Enzyme works at constant temperature
B. Allow The heart to pump the blood
C. Allow Water climbs up through xylem vessel
D. Water able to dissolve polar compounds
35. Which of the following is incorrect about the properties of water?
A. Able to exist in three states
B. It is a vital constituent of a living cell
C. It is more viscous than other fluid
D. It is the medium for metabolic reaction
36. Which spectra of light penetrate deep in to the ocean?
A. Green
B. Yellow
C. Violet
D. Blue
37. Which of the following is the function of carbohydrate?
A. Serve as a substrate for respiration
B. Serve as the structural component of animal, fungi, plant and exoskeleton of insects
C. Serve as the storage form of energy for plant animal and other organisms
D. All of the above
38. The process by which disaccharide are produced is
A. Condensation reaction between two monosaccharide sugar
B. Hydrolysis of monosaccharide sugar
C. Hydrolysis of polysaccharide
D. Condensation reaction of the two amino acids
39. Which of the following carbohydrate can produce more glucose upon hydrolysis?
A. Sucrose
B. Maltose
C. Starch
D. Glucose
40. Which polysaccharide are the most abundant in nature
A. Starch
B. Glucose
C. Cellulose
D. Glycogen
41. Which of the following is generally expected to give better health benefit when present in
human diet?
A. Animal fat
B. Saturated fatty acids
C. Polyunsaturated fatty acids
D. Monounsaturated fatty acids
42. Oils are liquid at room temperature, this is because of
A. They are made up of unsaturated fatty acids
B. They are made up of saturated fatty acids
C. They are mainly composed of long chain of glycerol
D. They have high proportion of oxygen
43. The sugars that make nucleic acids are
A. Glucose and fructose
B. Glyceraldehydes and dihydroxyacetate
C. Ribose and Deoxyribose
D. Starch and glycogen
44. Which of the following is true?
A. The bond between carbohydrates is glycosidic bond
B. The monomers of nucleic acids are nucleotides
C. The monomers of protein are amino acids
D. The bonds between two amino acids are peptide bond
E. The bond between fatty acid and glycerol in lipids is ester bond
F. All of the above
45. The two strand of a DNA double helix are held together by
A. Ionic bond
B. Disulphide bond
C. Hydrogen bond
D. Easter bond
46. Which of the following elements are mainly found in living organism?
A. Calcium, hydrogen, nitrogen, and oxygen
B. Carbon, hydrogen, nitrogen, and oxygen
C. Hydrogen, nitrogen, oxygen, and helium
D. Carbon, helium, nitrogen, and oxygen
E. Carbon, calcium, hydrogen, and oxygen
47. Solutes that easily dissolve in water are said to be
A. Hydrophobic
B. Hydrophilic
C. Polar molecules
D. All of the above
E. b and c only
48. Reactions in which water is used to break apart other molecules are known as __________
reactions.
A. Hydrophilic
B. Hydrophobic
C. Dehydration
D. Anabolic
E. Hydrolysis
49. Molecules that are found in living cells and contain the element___________ are considered
organic molecules.
A. Hydrogen
B. Carbon
C. Oxygen
D. Nitrogen
E. Calcium
50. A polysaccharide that is commonly found in plant cells and stores energy is
A. Glucose
B. Sucrose
C. Glycogen
D. Starch
E. Cellulose
51. Which of the following statement is not true about water?
A. It release a large amount of heat when changing from liquid into vapor
B. The solid form is less dense than the liquid form
C. It is the most effective solvent for polar molecules
D. It is typically the most abundant substance in a living organism
E. It takes part in some important chemical reactions
52. The most abundant molecule in the living organism is
A. Carbohydrate
B. Lipid
C. Nucleic acid
D. Protein
E. Water
53. All carbohydrate are;
A. Polymers
B. Simple sugar
C. Consist of one or more simple sugar
D. Found in biological membrane
E. More soluble in nonpolar solvent than water
54. An enzyme called amylase in the saliva facilitates the breakdown of carbohydrates into
which of the following compounds?
A. Fatty acids
B. Polypeptides
C. Amino acids
D. Simple sugars
55. Our body needs vitamins and minerals in small amount because they do have one of the
following functions in the body. Which one?
A. They can carry out metabolic reactions
B. They insulate the organs
C. They withdraw heat from the body
D. They provide the body with energy
56. A type of reaction which occurs when two or more sugars join together to form the double
and complex sugars is known as
A. Hydrolysis reaction
B. Condensation reaction
C. Addition reaction
D. Elimination reaction
57. Which of the following groups of sugars represent storage polysaccharide molecules?
A. Glycogen, cellulose and chitin
B. Glycogen, starch and cellulose
C. Glycogen and starch only
D. Glycogen, starch and chitin
58. All of the following are reducing sugars except:
A. Sucrose
B. Glucose
C. Maltose
D. Lactose
59. Which of the following is the result of hydrolysis of lactose?
A. One molecule of glucose and one molecule of fructose
B. One molecule of glucose and one molecule of Galactose
C. One molecule of fructose and one molecule of Galactose
D. Two molecules of glucose
60. Which of the following groups of substances are all organic?
A. Water, sugar, calcium carbonate
B. Water, calcium carbonate, carbon dioxide
C. Sugar, fatty acid, amino acid
D. Carbon dioxide, amino acid, fatty acid
61. Sweating would be effective in controlling the human body temperature. Which property of
water is highly suited to this importance?
A. High specific heat capacity
B. High latent heat of vaporization
C. Transparency
D. High surface tension
62. Alemu was conducting a research to investigate the effect of tomato juice on the germination
of tomato seeds. He placed 20 seeds in a Petri dish on filter paper and covers them with the
tomato juice (20ml) obtained from filtering the tomatoes. He also placed 20 seeds in a Petri dish
on filter paper and covers them with the same volume of distilled water. Finally, he observed that
only 5 seeds germinated in the tomato juice and 17 germinated in the distilled water. If Alemu
wants to write the report of this experiment, he should write
A. Something in tomato juice inhibits the germination of seeds as his hypothesis
B. The blended tomatoes material was filtered through and the juice was collected as his
prediction
C. The experiment was only carried out for 5 days and at one temperature with their minor
effects on reliability of the experiment as his evaluation
D. His name in the acknowledgement part
63. A grade 12 student hypothesized that the amount of sunlight a sunflower plant receives
determines the number of sunflower seeds the plant produces. In his/her experiment, which one
of the following is the dependent variable and independent variable respectively?
A. Amount of light, number of seeds
B. Amount of soil, number of seeds
C. Type of nutrients, amount of light
D. Number of seeds, amount of light
64. In a scientific experimental study, conclusions are drawn directly after -----
A. The hypothesis is revised
B. Results have been communicated to other scientists
C. Data have been interpreted
D. A clear description of the experimental procedure has been given
65. Which one of the following best describes the primary structure of proteins? It is
A. 3D structure
B. Alpha and beta sheets
C. Coiling and folding in form of specific structure
D. Number of amino acids in a chain
66. Which one of the following is the most abundant element in protein molecules?
A. Carbon
B. Hydrogen
C. Oxygen
D. Nitrogen
67. Which one of the following bond is mainly present in the primary structure of proteins?
A. Hydrogen bond
B. Peptide bond
C. Disulfide bond
D. Glycosidic bond