Glucose methodologies

 Chemical methods
o Oxidation reduction method
 Alkaline copper reduction method
 Folin wu method
 Benedict’s method
 Neocuproine method
 Nelson Somogyi method
 Alkaline ferric reduction method
 Hagedorn Jensen method
o Condensation method
 Dubowski method

Glucose methodologies

 Chemical method
o Oxidation reduction method
 Alkaline copper reduction method
 Folin Wu method
o Cupric ion > cuprous ion > cuprous oxide + phosphomolybdic
acid > phosphomolybdous > blue
 Benedict’s method
o Reducing sugar + benedict’s reagent then heated > reduction
reaction > brick red
o Fehling’s principle: Oxidized aldolases > carboxylic acid > cupric
ion + tartrate > cuprous oxide > brick red
 Nelson somogyi method
o Reducing sugars heated with alkaline copper tartrate cupric ion
> cuprous oxide + arsenomolybdate > molybdic acid >
molybdenum blue
 Neocuproine method
o Cuprous ion + neocuproine > cuprous-neocuproine complex +
chloroform methanol mixture > yellow or yellow orange
 Alkaline ferric reduction method
 Hagedorn Jensen method
o Reduction of yellow ferricyanide to colorless ferrocyanide
o Condensation method
 Dubowski method
 Condensation of glucose with primary aromatic amine > glycosylamine +
schiff’s base
 Glucose + ortho-toluidine > green compound
 Enzymatic method