media catal oxid TSI/la H2S GAS ONP dea LDC ODC ADH Motilit ureas IMVC

ctose y SIM e
ase ase G mina
fermen
ter? se INDOL MR VP CITRA
E TE

Escherichia coli EMB (black), + a/a - + + - + + - + - + + - -

PLM (yellow),
BAP (b), MAC most most -
(pink) EIEC

Enterobacter aerogenes MAC (pink) - a/a - - + + - + + - - + +

cloacae _ +

Klebsiella pneumoni MAC (mucoid ++ a/a - ++ - + - - - weak - - + +

ae pink)
+
oxytoca +

Serratia marcescen MAC (pink) + k/a - + - + - + - - + +

s slow

Proteus mirabilis MAC - k/a + + - - + - + - + v v

(colorless)
BAP (Swarm)
vulgaris k/a v - + -
d-xylose
only

Yersinia enterolitica MAC - k/a - - + 22C +

(colorless), - 37C
CIN (bullseye)

pestis MAC + a/k - - -

(colorless),
SB and Choc
(fried eggs)

Providencia retgerri Christensen - - + - - - + + - +

urea +

stuartii -

Morganella morganii KCN, PAD + k/a - + + + + + + - -

Edwardsiella tarda k/a + + - - + + - + - + + - -

Citrobacter Freundii Christensen LD - a/a + + + - - v v + v - + - +

KCN+ urea +
k/a

Diversus k/a - +
KCN -
Salmonella - LD + k/a + + - + + + + - - + - +

Shigella - -

TEST pH indicator POSITIVE NEGATIVE

TSI: Triple Sugar Iron Phenol red (yellow to orange) [k/a] red/yellow: no lactose [k/k]: no fermentation
[a/a] y/y: fermented all sugars

H2S black none

GAS Bubbles or cracks none

ONPG: Yellow: has B-galactosidase none

Orno-nitrophenyl-B-D-galactopyrano
side

LIA: Lysine Iron Agar Bromocresol purple (yellow to [k/a]: purple/yellow: + deam - decarb Note:
purple) [k/k]: p/p: - deam + decarb Deam: purple -
[r/a]: + deam - decarb Decarb: purple +

LDC: lysine Bromocresol purple or cresol red Purple: decarboxylation Yellow: decarboxylation

ODC: ornithine

ADH: arginine

Motility sim turbid line

urease Phenol red Pink: urease producer rapid yellow

Red: urease producer slow

IMVC indole Red ring: tryptophanase enzyme Yellow ring

IMVC methyl red Methyl red (red to yellow) Red: pyruvic acid Yellow

IMVC voges proskauer Red Yellow (no change)

IMVC citrate Bromothymol blue Blue Green (no change)