OQUENDO, MARIA KRISSHA ABBY D.

BSP2A

Test
Analysis of

Lipids

Acrolein Test Liebermann

Phosphate Test
-Burchard test

All of the three test will require the following samples: (A) Butter, (B) Cholesterol,

(C) Corn oil, (D) Egg yolk, (E) Glycerine, (F) Lanolin, (G) Lecithin, (H) Margarine

Prepare eight clean and dry test tubes.

Prepare eight clean and dry test
Prepare eight clean and dry test

Label the test tubes accordingly, from A to

tubes. Label the test tubes
tubes. Label the test tubes

H. accordingly, from A to H. accordingly, from A to H.

Place about 0.2 g of sample to its

Place about 0.2 g of sample to its

Place about 0.2 g of sample to its

respective test tube. Then add 1.0 g
respective test tube. Then add 3.0

respective test tube. Then add 3.0 mL of 6

of potassium bisulfate to all the
mL chloroform and 1.0 mL acetic

M nitric acid to all the samples. samples. anhydride to all the samples.

Set up your Bunsen burner in the

Meanwhile, prepare a boiling water bath

fume hood. Add 1 drop of concentrated sulfuric

of 100 mL distilled water in a beaker.

acid to all the samples. Mix the

Place all the test tubes in the boiling

contents.
water bath for 5 minutes. Be careful not

Gently heat each test tube over the

inhale the vapors.

Bunsen burner flame, shaking it

continuously from side to side.

Observe for any changes in the color

When the mixture melts, it will

of the solution. The change in color

Remove from heat and set aside to cool.

may appear first as purple to pink

slightly blacken. Stop the heating

Neutralize the samples by adding 3.0 mL

then progresses to light green to

when fumes start to appear. Do not

of 6 M sodium hydroxide. Shake all the

dark green. Record results.
overheat the test tubes for the

test tubes. Precipitates may form during

residue will harden, making it

this step. Remove the precipitates by

difficult to clean the test tubes.

filtering the solution using a cheeseclot

Transfer 2.0 mL of each neutralized

Carefully smell the test tubes.

sample into clean and labeled test tubes. Observe proper method of smelling

fumes from a test tube. A pungent

acrid odor that resembles a burnt

Add 3.0 mL of ammonium molybdate to

meat is the positive result. Record

each test tube and mix the contents. results.

Heat the test tubes in a boiling water bath

for 5 minutes. Remove from heat and set

aside to cool.

Then add 0.5 mL of ascorbic acid solution

to each test tube and mix the contents.

Wait for 20 minutes for purple color to

develop. Record results.