Objective. To study different water samples for the presence of living organ- isms. REQUIREM. Water samples (such as pond water, river water, canal water ete.) microscope, glass slides, dropper, methylene blue, spirit lamp, ete. PROCEDURE ‘Take a clean slide and put a few drop of water seperately from different water samples. Spread the drops to make a thin film of water on the slide. Allow it to dry. Pass the lower side of the slide through the flame of spirit lamp two or three times to fix the living organisms present in water on to the slide. Add a few drop of a methylene blue on the slide. Leave the slide for two minutes. Wash the slide and observe the slide under the microscope. OBSERVATION A number of types of microorganisms (such as bacteria, protozoa, diatoms, some algae, cyanobacteria) are observed. Different types of organisms present in water samples are given in Figs. 4.2 and 4.3. CONCLUSION Presence of large number of microorganisms indicates the presence of organic pollutants in water.1. Shake the water well before putting the drops of water on the slide from it, 2. Paws the slide through the flame only to get it dry.