Archive of SID

2nd National Congress on Medicinal Plants

15, 16 May 2013
Tehran- Iran

A NEW METHOD BASED ON THE UV-VISIBLE SPECTROSCOPY FOR

QUANTITATIVE ANALYSIS OF ATROPINE IN BIOLOGICAL
EXTRACTS

Akbar Abbaspour,1 Parissa Khadiv-Parsi,1,* Farahnaz Khalighi-Sigaroodi,2

Reza Ghafarzadegan2
1
School of Chemical Engineering, College of Engineering, University of Tehran, Tehran, Iran
2
Department of Pharmacognosy & Pharmaceutics, Institute of Medicinal Plants, ACECR,
Karaj, Iran
E-mail: kparsi@ut.ac.ir

Several methods for atropine tropane alkaloids analysis have been reported in the
literature such as gas chromatography (GC), liquid chromatography (LC), and capillary
electrophoresis (CE). A new method, namely, a double standard-analyte addition
method using UV-visible spectroscopy based on the chloranilic acid-atropine
complexometery, was designed for quantitative analysis of atropine in biological
extracts [1]. Indeed, the advantage of the standard addition method than other methods
is to apply it for unknown solution matrix. However, this standard method cannot be
used for the cases that the other unknown samples participate in the total absorption.
This defect has been resolved in the new method which we have used in this work. In
the new method, analysis of atropine in biological extracts was performed by UV-
visible complexometery of the atropine-chloranilic acid complexes at λmax = 535 nm in
cholorform-dioxane mixed medium [2]. The comparison of this new method and the
liquid chromatography (LC) method confirmed the performance of this method.

References
[1] Elsayed, M. A.; Agarwal, S.P. Talanta, 1982, 29, 535-537.
[2] Ashtiani, F.; Sefidkon, F. J. Med. Plants Res. 2011, 5(29), 6515-6522.

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