Professional Documents
Culture Documents
CERTIFICATION EXAM
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748 18 0 3
What i s not an external def ense mechani sm of natural i mmuni ty that provi des the
Bursa of F abri ci us
human body wi th a def ense agai nst i nvadi ng mi croorgani sms?
Whi ch def ense mechani sm i nvol ves engul f i ng f orei gn parti cl es? phagocytosi s
What pl ays an i mportant rol e i n maki ng bacteri a suscepti bl e to osmoti c l ysi s? l ysoz ymes
ASCP CERTIFICATION EXAM
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748 18 0 3
Recei vi ng a vacci nati on i s an exampl e of what type of i mmuni ty? arti f i ci al acti ve i mmuni ty
Whi ch organs or systems are capabl e of respondi ng to ci rcul ati ng anti gens wi th
l ymph nodes and spl een
the producti on of speci f i c l ymphocytes and pl asma cel l s?
H ow can cel l -medi ated i mmuni ty be transf erred to a normal i ndi vi dual ? wi th l ymphoi d cel l s f rom a hypersensi ti ve i ndi vi dual
What i s the mai n f uncti on of T -hel per cel l s? assi st B cel l s i n produci ng anti body
ASCP CERTIFICATION EXAM
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748 18 0 3
What are smal l pol ypepti des gi ven of f by T cel l s, whi ch regul ate the f uncti on of
l ymphoki nes
phagocyti c cel l s cal l ed?
Whi ch T cel l acts speci f i cal l y to ki l l tumor cel l s and vi ral l y i nf ected cel l s? cytoxi c T cel l s
Lymphoi d stem cel l s di f f erenti ate i n the bone marrow are cal l ed what? B cel l s
What cel l s are i denti f i ed by the presence of surf ace i mmunogl obul i n receptor? B cel l s
Whi ch B l ymphocytes transf orm i nto cel l s that produce anti body mol ecul es? pl asma cel l s
ASCP CERTIFICATION EXAM
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748 18 0 3
Whi ch whi te bl ood cel l s mi grate to the ti ssue and become macrophages? monocytes
What i s the mai n f uncti on of pol ymorphonucl ear whi te bl ood cel l s? phagocytosi s
What i s the maj or hi stocompati bi l i ty compl ex? the genes that control expressi on of the H LA anti gens
H LA-D P
What are cl ass I I gene products? H LA-D Q
H LA-D R
What are the reacti ve si tes on an anti gen known as? determi nants
What does a heterol ogous anti gen react wi th? an anti body whose producti on i s i nduced
M ost anti bodi es mi grate i n whi ch regi on duri ng serum protei n el ectrophoresi s? gamma
ASCP CERTIFICATION EXAM
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748 18 0 3
What cel l s i ngest and destroy bacteri a, damaged host cel l s, and tumor cel l s? macrophages
Whi ch i mmunogl obul i n i ncreases duri ng the conval escence phase of i nf ecti on? I gG
ASCP CERTIFICATION EXAM
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748 18 0 3
I gM
What i s the f i rst anti body to be produced i n i nf ecti ons?
What represents the rel ati ve concentrati on of i mmunogl obul i ns decreasi ng order i n
I gG, I gA, I gM , I gD , I gE
normal serum?
M easurement of serum l evel s of whi ch of the f ol l owi ng i mmunogl obul i ns can serve
I gE
as a screeni ng test f or al l ergi es?
ASCP CERTIFICATION EXAM
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748 18 0 3
What i s the basi c i mmunogl obul i n structure? 4 heavy chai ns + 4 l i ght chai ns
Whi ch regi on of the i mmunogl obul i n i s responsi bl e f or anti gen bi ndi ng? vari abl e regi on
I n the structure of i mmunogl obul i ns, whi ch regi on contai ns the di sul f i de bri dge? constant regi on
Anti body cl ass i s determi ned by the anti geni c vari ati on f ound pri mari l y i n whi ch
constant regi on of the heavy chai n
regi on?
ASCP CERTIFICATION EXAM
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748 18 0 3
What i s a proteol yti c enz yme that f ragments I gG i n three f ragments? papai n
Af ter an anti body has been f ragmented by papai n, whi ch term denotes the anti gen-
F ab
bi ndi ng f ragment?
T he area of i mmunogl obul i n mol ecul e ref erred to as the hi nge regi on i s l ocated
CH 1 and CH 2
between whi ch domai ns?
What i s the f i rst i mmunogl obul i n cl ass to appear i n the pri mary response? I gM
A rapi d anti body producti on to an el evated l evel that persi sts f or a l ong ti me that
f ol l ows repeated exposure to an anti gen i s seen i n whi ch phase of the i mmune anamnesti c response
response?
ASCP CERTIFICATION EXAM
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748 18 0 3
the end resul t of compl ement f i xati on i s cel l l ysi s by membrane damage
What i s true concerni ng compl ement? Both I gG and I gM anti bodi es bi nd compl ement
Compl ement acts as an opsoni n
Whi ch term best descri bes a smal l pepti de f ormed duri ng compl ement acti vati on
that causes i ncreased vascul ar permeabi l i ty, contracti on of smooth muscl e, and anaphyl atoxi n
rel ease of hi stami ne f rom mast cel l s?
Whi ch compl ement component of the recogni ti on phase i s the f i rst component to
C1q
bi nd to the i mmunogl obul i n?
What i s the order of acti vati on of compl ement components i n the cl assi cal
C1, C4, C2, C3
pathway?
Whi ch compl ement compl ex i s known as the membrane attact uni t? C56789
Whi ch compl ement component i s present i n the hi ghest concentrati on i n serum and
C3
i s a key component i n both the cl assi cal and al ternati ve pathways?
Whi ch one of the compl ement components i s N OT i nvol ved i n the al ternate
C1
pathway?
What i s the end product of compl ement acti vati on? cel l l ysi s
pH
Anti gen-anti body bi ndi ng i s af f ected by what? temperature
l ength of the i ncubati on
ASCP CERTIFICATION EXAM
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748 18 0 3
Whi ch reacti on i s caused by an I gG anti body bi ndi ng to the surf ace of a red
sensi ti z ati on
bl ood cel l causi ng a reversi bl e reacti on?
Combi nati on of sol ubl e anti body wi th sol ubl e anti gen to produce i nsol ubl e
preci pi tati on
compl exes i s the pri nci pl e of whi ch method?
T he opti mal rati o, when the most anti body i s preci pi tated by the l east amount of
z one of equi val ence
anti gen, i s ref erred to as whi ch of the f ol l owi ng?T he
ASCP CERTIFICATION EXAM
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748 18 0 3
What are hemaggl uti nati on tests? aggl uti nati on tests i n whi ch the anti gens are l ocated on the red bl ood cel l s
H ow are monocl onal anti bodi es prepared? f use B l ymphocytes wi th a pl asma myel oma cel l
react wi th onl y one anti geni c determi nant on a mul ti val ent anti gen
N ame some l i mi tati ons of monocl onal anti bodi es f rom a hybri doma? are not good i n preci pi tati on of hemaggl uti nati on assays
do not cross-l i nk to f orm a preci pi tate
Why can't monocl onal anti bodi es be used i n compl ement f i xati on tests? T hey cannot f i x compl ement
ASCP CERTIFICATION EXAM
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748 18 0 3
I n serol ogi cal seri al di l uti on, i f tube #1 has 1 mL of an undi l uted speci men and
512
doubl i ng di l uti ons are used thereaf ter, what i s the ti ter i n tube #10?
What term i s used to descri be the abi l i ty of a cl i ni cal test to be posi ti ve i n the
sensi ti vi ty
absence of i ts homol ogous anti gen?
Gi ven the f ol l owi ng resul ts, what i s the anti body ti ter?
1:1 1:2 1:4 1:8 1:16 1:32 1:64 8
pos pos pos pos neg neg neg
What phenomenon occurs when an anti body reacts wi th an anti gen that i s
cross-reacti vi ty
structural l y si mi l ar to the ori gi nal anti gen that i nduced anti body producti on?
ASCP CERTIFICATION EXAM
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748 18 0 3
What term i s used to descri be the bi ndi ng strength of an anti body f or an anti gen? avi di ty
T he random movement of anti gen or anti body to f orm anti gen-anti body compl exes
i mmunodi f f usi on
i n semi sol i d medi um occurs i n whi ch i mmunol ogi cal techni que?
Anti gen and anti body are both f ree to move toward each other to f orm a
two-di mensi onal di f f usi on
preci pi tate i n whi ch i mmunol ogi cal techni que?
l i ne of f usi on
I n doubl e radi al di f f usi on, what shows that the anti gens are i denti cal ? l i ne of segregati on
l i nes of parti al i ntersecti on
ASCP CERTIFICATION EXAM
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748 18 0 3
E l ectroi mmunodi f f usi on i s di rected movement of anti gen or anti gen and anti body
semi sol i d medi a
i n whi ch type of medi a?
ASCP CERTIFICATION EXAM
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748 18 0 3
What i s another name f or one-di mensi onal si ngl e el ectroi mmunodi f f usi on? rocket el ectrophoresi s
Why have enz yme i mmunoassays repl aced radi oi mmunoassays? there are haz ards and regul ati ons associ ated wi th radi oi mmunoassay
Whi ch f l uorochrome gi ves of f a red emi ssi on at 580 nm? tetramethyl rhodami ne i sothi ocynate
I n the enz yme-l i nked i mmunosorbent assay (E LI SA), whi ch of the f ol l owi ng can be
anti body or anti gen
attached to a sol i d phase support (e.g., pol ystyrene)?
ASCP CERTIFICATION EXAM
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748 18 0 3
I n the enz yme-l i nked i mmunosorbent assay (E LI SA), what i s the anti human gl obul i n
horseradi sh peroxi dase
conj ugated wi th?
Whi ch i mmunogl obul i n i s measured i n the radi oal l ergosorbent (RAST ) test? I gE
Compl ement f i xati on tests contai n whi ch two systems? test and i ndi cator systems
What i s the i ndi cator i n the compl ement f i xati on test? sheep red bl ood cel l s
ASCP CERTIFICATION EXAM
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748 18 0 3
What i s used to sensi ti z e red bl ood cel l s i n the compl ement f i xati on test? anti sheep hemol ysi n
What i s the purpose of anti sheep hemol ysi n i n a compl ement f i xati on test? sensi ti z es sheep red bl ood cel l s
What shoul d occur when control s f or compl ement f i xati on tests are used to check
hemol ysi s
f or anti compl ementary f actors?
I n the compl ement f i xati on test, the posi ti ve control shoul d show whi ch reacti on? no hemol ysi s
ASCP CERTIFICATION EXAM
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748 18 0 3
H ow i s a posi ti ve pati ent reacti on expressed i n a compl ement f i xati on test? i nhi bi ti on of hemol ysi s
I n a negati ve compl ement f i xati on test, what wi l l happen to the red bl ood cel l s? hemol yz e
Pati ents wi th syphi l i s devel op an anti body response to whi ch substance? cardi ol i pi n
What rol e does cardi ol i pi n pl ay i n the VD RL test? I t i s the nontreponemal anti gen
ASCP CERTIFICATION EXAM
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748 18 0 3
What reacti on occurs between the anti body and the anti gen i n the VD RL test? f l occul ati on
H ow of ten shoul d needl es used to del i ver VD RL anti gen be checked? dai l y
H ow much does the anti gen needl e f or the qual i tati ve VD RL test del i ver? 60 drops/mL
H ow much shoul d sal i ne needl es f or quanti tati ve VD RL test del i ver? 100 drops/mL
ASCP CERTIFICATION EXAM
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748 18 0 3
I nacti vati on of Sera f or serol ogi cal tests i s perf ormed f or what purpose? destructi on of compl ement
I f the i nterval between heati ng the serum f or the VD RL test and testi ng exceeds 4
56 degrees C f or 10 mi nutes
hours, how l ong shoul d the serum be reheated f or?
I f the VD RL test shows smal l cl umps of anti gen wi th many f ree parti cl es, how
weakl y reacti ve
shoul d i t be reported?
When i s the quanti tati ve VD RL test perf ormed? to ti ter reacti ve and weakl y reacti ve resul ts
What represents the reported ti ter i n a quanti tati ve VD RL test? hi ghest di l uti on gi vi ng a reacti ve resul t
What i s true concerni ng the RPR test? I t does not requi re the i nacti vati on of serum
ASCP CERTIFICATION EXAM
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748 18 0 3
What i s the anti gen used i n the RPR test? cardi ol i pi n wi th added charcoal
T he RPR anti gen needl e wi l l del i ver how many drops? 60 drops/mL
T he RPR test i s rotated at what speed f or what l ength of ti me? 100 rpm f or 8 mi nutes
ASCP CERTIFICATION EXAM
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748 18 0 3
What i s a requi rement of the T reponema pal l i dum i mmobi l i z ati on (T PI ) test? l i vi ng T reponema pal l i dum
What serves as the absorbent i n the f l uorescent treponemal anti body absorpti on
T reponema pal l i dum, Rei ter strai n
(F T A-ABS) test?
T he F T A-ABS test i s used to i denti f y what i n the pati ent's serum? treponemal anti body
What i s the purpose of the anti body conj ugate (anti -i mmunogl obul i n wi th a
makes the anti gen-anti body reacti on vi si bl e
f l uorescei n i sothi ocynate l abel ) i n the F T A-ABS test?
ASCP CERTIFICATION EXAM
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748 18 0 3
Whi ch test i s l ess l i kel y to be reacti ve i n pri mary and earl y secondary syphi l i s? M H A-T P
I n nontreponemal tests, serum that gi ves a reacti ve serol ogi cal test that i s not
f al se posi ti ve
caused by syphi l i s resul ts i n what?
What does the anti streptol ysi n O (ASO) test measure i n the pati ent's serum? anti body to streptol ysi n O
ASCP CERTIFICATION EXAM
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748 18 0 3
What i s a posi ti ve reacti on i n the anti streptol ysi n O sl i de test? aggl uti nati on
What i s the pri nci pl e of the anti streptol ysi n O tube test? hemol ysi s-i nhi bi ti on
I n the cl assi c anti streptol ysi n O (ASO) test, why shoul d streptol ysi n O not be
i s destroyed by oxygen
rehydrated unti l i t i s needed?
H ow i s the serum ti ter i n the anti streptol ysi n O (ASO) test reported? T odd or i nternati onal uni ts
ASCP CERTIFICATION EXAM
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748 18 0 3
Whi ch streptococcal extracel l ul ar product di ssol ves f i bri n cl ots? streproki nase
H ow i s the heterophi l anti body reported? hi ghest di l uti on that shows aggl uti nati on
ASCP CERTIFICATION EXAM
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748 18 0 3
What type of red bl ood cel l i s used i n the Paul -Bunnel heterophi l anti body test? sheep
I n the heterophi l anti body test, whi ch of the f ol l owi ng consti tutes a posi ti ve
aggl uti nati on
reacti on?
What i s the pri nci pl e of the D avi dsohn heterophi l anti body test? di f f erenti ates three types of heterophi l anti bodi es
ASCP CERTIFICATION EXAM
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748 18 0 3
T he D avi dsohn di f f erenti al test of uses what type of bl ood cel l s? horse
Whi ch anti gen i s used to determi ne the somati c anti geni c groupi ng of enteri c
O
bacteri a?
I n the Wi dal test, what does an el evated O ti ter i ndi cate? i ncrease i n aggl uti ni ns to Sal monel l a typhi
T he Wei l -F el i x test i s used f or the detecti on of whi ch type of anti bodi es? Ri ckettsi a
ASCP CERTIFICATION EXAM
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748 18 0 3
What are the OX19 and the OX2 anti gens? strai ns of Proteus vul gari s
What do the Wi dal and Wei l -F el i x tests detect? f ebri l e aggl uti ni ns
Anti bodi es to Brucel l a abortus wi l l cross-react wi th whi ch other organi sm? F ranci sel l a tul arensi s
T he ti ter must be greater than 1:512 i n order to di agnose whi ch f ungal di sease? aspergi l l osi s
ASCP CERTIFICATION EXAM
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748 18 0 3
T he cryptococcal anti gen test i s rapi dl y repl aci ng whi ch l ab test? I ndi a i nk
What can i nterf ere wi th the cryptococcal anti gen l atex aggl uti nati on test? rheumatoi d f actor
T he rheumatoi d f actor (RF ) l atex sl i de test uses di l uted pati ent's serum pl us
anti human gl obul i n
l atex coated wi th what?
What i s true concerni ng the rheumatoi d f actor l atex test? Synovi al f l ui d may be the source of anti body
ASCP CERTIFICATION EXAM
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748 18 0 3
What anti bodi es i s di rected agai nst the F c porti on of the I gG? C-reacti ve protei n
What does the presence of C-reacti ve protei n i n a pati ent's serum i ndi cate? i nf l ammati on
Whi ch pattern of f l uorescence i n the anti -nucl ear anti body (AN A) test i s most
homogenous
cl osel y associ ated wi th acti ve systemi c l upus erythematosus (SLE )?
A posi ti ve anti -ri bonucl eoprotei n (anti -RN P) i n the anti nucl ear anti body group i s
mi xed connecti ve ti ssue di sease
suggesti ve of whi ch autoi mmune di sease?
ASCP CERTIFICATION EXAM
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748 18 0 3
What i s the maj or autoanti gen of the thyroi d gl and? thyrogl obul i n
Anti -gl omul ar basement membrane anti bodi es are associ ated wi th whi ch
Goodpasture's syndrome
autoi mmune di sease?
What i s the i ncubati on temperature f or a pati ent sampl e i n the col d aggl uti ni n
4 degrees C
test?
What col d reacti ng anti bodi es i s present i n i nf ecti ons of M ycopl asma
anti -I
pneumoni ae?
ASCP CERTIFICATION EXAM
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748 18 0 3
Col d aggl uti ni ns may devel op af ter an i nf ecti on caused by whi ch organi sm? M ycopl asma pneumoni ae
Whi ch type of hepati ti s i s associ ated wi th eati ng raw shel l f i sh? hepati ti s A
H epati ti s vacci nati on f or heal thcare workers protects agai nst whi ch vi ral agent(s)? H BV
Whi ch herpes si mpl ex vi rus (H SV) can cause urogeni tal i nf ecti ons? both H SV-1 and H SV-2
Whi ch method i s used to serotype the herpes si mpl ex vi rus (H SV)? di rect f l uorescent anti body method
Whi ch method has a hi gh degree of speci f i ci ty when typi ng the herpes si mpl ex
enz yme-l i nked i mmunosorbent assay
vi rus (H SV)?
What i s the most common serol ogi cal test f or vari cel l a z oster vi rus (VZ V)? enz yme-l i nked i mmunosorbent assay test
Cytomegal ovi rus (CM V) i nf ecti on can be di agnosed by what? I gM and I gG anti body tests
Whi ch test f or cytomegal ovi rus has probl ems wi th f al se posi ti ves? i ndi rect i mmunogl uorescence
What i s a good screeni ng test f or determi ni ng i mmuni ty to the rubel l a vi rus? I gG anti body test
ASCP CERTIFICATION EXAM
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748 18 0 3
Whi ch serol ogi cal method i s routi nel y used f or rubel l a testi ng? enz yme-l i nked i mmunosorbent assay test
What i s the most popul ar serol ogi cal test f or the di agnosi s of T oxopl asma
enz yme-l i nked i mmunosorbent assay test
gondi i ?
T he M antoux ski n test i s an exampl e of whi ch type of hypersensi ti vi ty reacti on? type I V
AI D S pati ents characteri sti cal l y show a def i ci ency i n what? T l ymphocytes
sexual contact
H I V i nf ecti on i s transmi tted i n whi ch ways? bl ood transf usi ons
shari ng hypodermi c needl es
What i s the most commonl y perf ormed conf i rmatory method f or H I V i nf ecti on? Western bl ot
What i s an i mmune def i ci ency characteri z ed by thrombocytopeni a at bi rth? Wi skott-Al dri ch syndrome