BIOCHEMISTRY

Activity 6
Enzyme Hydrolysis of Starch
Group Number: Section:
Group Leader:
Group Members:

Please complete the following tables.

Effect of pH
pH Change in colour of iodine at different time interval (minute)
1 2 3 4 5 6 7 8 9 10
4.0
7.0
10.0

Effect of Temperature
Temperature Change in colour of iodine at different time interval (minute)
(°C) 1 2 3 4 5 6 7 8 9 10
0
37
70

Effect of Disinfectant
Change in colour of iodine at different time interval (minute)
2.5 5.0 7.5 10.0
w/o Lysol
w/ Lysol

Guide Questions

1. Are all enzymes in nature work best at pH 7 and 37 °C? Explain

2. Explain why heat, cold, and disinfectants prevent microbial growth.

3. What is the optimum condition of the α-amylase enzyme as regard to temperature? Did
we get the expected result? If not, provide an explanation as to why this was the case.

***NOTHING FOLLOWS***
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