Must know in Mycology

• Basic structure of fungi – hyphae and spore

• General culture media – SDA
• Czapek’s medium – Aspergillus
• Serum at 37OC – germ tube
• Cornmeal agar – Chlamydospore
• Media with olive oil – Malassezia furfur
• Hair baiting test – T. mentagrophytes
• Trichophyton – skin, hair, nails
• Red color, tear drop conidia – T. rubrum
• Favic chandelier (antler) – T. schoenleinii
• Coenocytic – Phycomycetes / Zygomycetes
• Ascospore – Saccharomyces cerevisiae
• C. neoformans – Niger birdseed (+), urease (+), phenol oxidase (+), India ink
• Arthrospore – Geotrichum, Coccidioides, Trichosporon, Aureobasidium
• Blastospores – yeast
• 10% KOH – direct microscopic, clearing
• LPCB – common fungal stain
• Wright / Giemsa – H. capsulatum
• Dichotomous hyphae – Aspergillus
• Thrush, diaper rash, Moniliasis – Candida
• Actinomyces – Sulfur granules
• Nocardia – modified acid fast
• Macro- microconida – Dermatophytes
• Sputum – systemic mycoses
• CHO assimilation – ID yeast, free of CHO
• BHIA- dimorphic fungi
• C. immitis – high risk to lab personnel
• Chlorampenicol and cycloheximide – inhibitory agent for mycosel / mycobiotic