Chart Title

4
3.5
3
2.5
2

lnX
1.5
1
0.5
0
0 5 10 15 20 25 30 35 4
Tiempo (h)

uneta 0.09090924 MSE 100.917016

Tiempo [celulas] [glucosa] lnX xpred uneta
0 1.25 100 0.22314355
9 2.45 97 0.89608802 2.83297318 0.14666846 0.07477161
16 5.1 90.4 1.62924054 5.3531837 0.06410199 0.10473607
23 10.5 76.9 2.35137526 10.1153713 0.14793921 0.1031621
30 22 48.1 3.09104245 19.113997 8.32901315 0.10566674
34 33 20.6 3.49650756 27.4964766 30.2887693 0.10136628
36 37.5 9.38 3.62434093 32.9791771 20.43784 0.06391669
40 41 0.63 3.71357207 47.4422577 41.5026838 0.02230778
 Chart Title Chart Title Chart Title
45 120
40
100
35
30 80
25

X (g/L)

S (g/L)
60
20
15 40
10
20
5
0 0
5 10 15 20 25 30 35 40 45 0 5 10 15 20 25 30 35 40 45 0 5 10 15 20 25 30 35 40
Tiempo (h) Tiempo (h) Tiempo (h)

Yx/s Xmax
61.2905048
0.4
0.40151515
0.4
0.39930556
0.4
0.40106952
0.4
0.40027003
g celulas/g sustrato
 Chart Title

5 10 15 20 25 30 35 40 45
Tiempo (h)
 Biomasa
12
10
8
6
4
2
0
0 5 10 15 20 25 30

K 0.26829202
MSE 0.41606281

Time [S] [X] [P]

0 100 0.5 0 Xpred ERR Yp/s Yx/s
2 95 1 2.5 0.82785954 0.02963234 0.5 0.1
5 85 2.1 7.5 1.69115961 0.16715047 0.5 0.11
10 58 4.8 20 4.48452379 0.09952524 0.46296296 0.1
15 30 7.7 34 7.89349837 0.03744162 0.5 0.10357143
20 12 9.6 43 9.85154512 0.06327495 0.5 0.10555556
25 5 10.4 47.5 10.5348141 0.01817483 0.64285714 0.11428571
30 2 10.7 49 10.7293829 0.00086336 0.5 0.1
10.8 0.51511716 0.10477324
 120
Biomasa
100

80

60

40

20

0
10 15 20 25 30 35 0 5 10 15 20 25 30 35

DT Xprom DX/(DT*Xpro1-xprom/xinfk
2 0.75 0.33333333 0.93055556 0.35820896
3 1.55 0.23655914 0.85648148 0.27619878
5 3.45 0.15652174 0.68055556 0.22999113
5 6.25 0.0928 0.4212963 0.22027253
5 8.65 0.04393064 0.19907407 0.22067482
5 10 0.016 0.07407407 0.216
5 10.55 0.0056872 0.02314815 0.2456872
0.25243334
La concentración total de biomasa será:
 S0 800

D S X 1/D X/(S0-S)=Yap
0.1 16.7 366 10 0.46725393 2.14016393
0.2 33.5 407 5 0.530985 1.88329238
0.3 59.4 408 3.33333333 0.55090467 1.81519608
0.4 101 404 2.5 0.57796853 1.73019802
0.5 169 371 2 0.58795563 1.70080863
0.6 298 299 1.66666667 0.59561753 1.67892977
0.7 702 59 1.42857143 0.60204082 1.66101695
 Chart Title 8
2.5
7 f(x) = 102.935906588031 x + 1.24034567270077
R² = 0.999776120282482
6
2 f(x) = 0.0554961520231285 x + 1.59580997351217
R² = 0.989876966780718 5

1.5 4

3
1 2

1
0.5
0
0 0.01 0.02 0.03 0.04 0.05 0.06 0.07

0 1/(D+kd) 1/S
1 2 3 4 5 6 7 8 9 10 11
7.41956481 0.05988024
Yx/sM ms kd 4.25932846 0.02985075
0.62664494 0.0555 0.03477879 2.98704702 0.01683502
2.30002018 0.00990099
1.86993204 0.00591716
1.57535193 0.0033557
1.36095381 0.0014245
+ 1.24034567270077 um 0.80625655
Ks 82.9960493

0.04 0.05 0.06 0.07

 Chart Title
7

X (g/L)
3

0
0 2 4 6 8 10 12 14 16 18 20
Tiempo (h)

t X lnX S Dt DX
0 0.2 9.23
2 0.211 9.21 2 0.011
4 0.305 -1.1874435 9.07 2 0.094
8 0.98 -0.02020271 8.03 4 0.675
10 1.77 0.57097955 6.8 2 0.79
12 3.2 1.16315081 4.6 2 1.43
14 5.6 1.7227666 0.92 2 2.4
16 6.15 0.077 2 0.55
18 6.2 0 2 0.05
x inf 6.25
 Chart Title
2
f(x) = 0.291927951642488 x − 2.35265818687983
1.5 R² = 0.999934903175819

0.5

lnX
umax aprox mediante grafica lnX vs t unicamente fase
0 exponencial
0 2 4 6 8 10 12 14 16 18 20
a) umax 0.2919
-0.5
b) Yx/s app 0.65005417
c) tau 2.37460494 -1
d) Ks 0.39280635 SOLVER -1.5
MSE 0.04903961 Tiempo (h)
e) unet(t=10) 0.28727273

Xprom DX/DT DX/(DT*xpro 1-xprom/xinfDX/DX pred error

7

0.2055 0.0055 0.02676399 0.96712 0.0590715 0.00286991 6

0.258 0.047 0.18217054 0.95872 0.08533384 0.00146948
5
0.6425 0.16875 0.26264591 0.8972 0.2727212 0.01081001
1.375 0.395 0.28727273 0.78 0.48844752 0.00873244 4
2.485 0.715 0.28772636 0.6024 0.86059176 0.02119696

X
3
4.4 1.2 0.27272727 0.296 1.14553742 0.00296617
5.875 0.275 0.04680851 0.06 0.29422601 0.00036964 2
6.175 0.025 0.00404858 0.012 0 0.000625
1

0
0 2 4 6 8 10 12
Tiempo (h)
8 x − 2.35265818687983

12 14 16 18 20

2 4 6 8 10 12 14 16 18 20
Tiempo (h)
 R, kcal/mol-K 0.0019872
Ea 4.65646494
Ad 17.4423515
MSE 0.03855516

m -8702.3
b 23.526

T, °C T, K k, mol/L-h k pred ERR lnk

40.4 313.4 0.014 0.0098735204 0.01702783 -4.26869795
36.8 309.8 0.0112 0.0090518853 0.0046144 -4.4918415
33.1 306.1 0.0074 0.0082610081 0.00074133 -4.90627528
30 303 0.00511 0.0076386969 0.00639431 -5.27655587
25.1 298.1 0.0036 0.0067268653 0.00977729 -5.62682143
Ea 17.29321056 kcal/mol
Ad 16489670640

1/T Chart Title

0.00319081
0
0.003227889 0.00315 0.0032 0.00325 0.0033 0.00335 0.0034
0.003266906 -1
0.00330033
0.003354579 -2

-3

-4
f(x) = − 8702.3023742169 x + 23.5259815658432
-5R² = 0.988425301459527

-6
 Cha
3.5
3
2.5
2
1.5
1
0.5
0
0 5 10
OUR X -0.5
Time (g/h) (g/l) lnX -1
0 0.011 0.6 -0.51082562
1 0.008 0.63 -0.46203546 umax
2 0.084 0.63 -0.46203546
3 0.153 0.76 -0.27443685 Chart Title
4 0.198 1.06 0.05826891
3
5 0.273 1.56 0.44468582
6 0.393 2.23 0.80200159 2.5
f(x) = 0.299543638654344 x − 1.04553
7 0.493 2.85 1.04731899 R² = 0.993180377064797
8 0.642 4.15 1.42310833 2
9 0.915 5.37 1.68082791
10 1.031 7.59 2.02683159 1.5
11 1.12 9.4 2.24070969
12 1.37 11.4 2.43361336 1
13 1.58 12.22 2.50307395
14 1.26 13 2.56494936 0.5
15 1.58 13.37 2.59301339
0
16 1.26 14.47 2.67207754 3 4 5 6 7 8 9 10 11
17 1.12 15.37 2.73241756
18 1.2 16.12 2.78006074
19 0.99 16.18 2.78377591
20 0.86 16.67 2.8136107
21 0.9 17.01 2.83380141
 Chart Title
3.5
3
2.5
2 FASE EXPONENCIAL
1.5
1
0.5
0
0 5 10 15 20 25
-0.5
-1
Yx/o2 2.23674384 g X/g O2
0.2995 h-1

Chart Title Chart Title

1.2

f(x) = 0.133949333185123 x + 0.0888167205253004

1 R² = 0.968012700582075
f(x) = 0.299543638654344 x − 1.04553064390305
R² = 0.993180377064797
0.8

0.6

0.4

0.2

0
5 6 7 8 9 10 11 12 13 0 1 2 3 4 5 6 7 8
FASE ESTACIONARIA

OUR=mo2*X

OUR X
Time (g/h) (g/l) ms
14 1.26 13 0.09692308
15 1.58 13.37 0.11817502
16 1.26 14.47 0.08707671
17 1.12 15.37 0.07286923
18 1.2 16.12 0.07444169
19 0.99 16.18 0.06118665
20 0.86 16.67 0.05158968
21 0.9 17.01 0.05291005
0.07689651
 Char
3.5

3 f(x) = 0.05778947742
R² = 0.936734117717
2.5

1/Yxsap
1.5

0.5

0
0 5 10

D, S, mg/ml X
h-1 mg/mL mg/mL
dilution rate 1/D glycerol 1/S cell conc DS DS/X
0.05 20 0.012 83.3 3.2 9.988 3.12
0.1 10 0.028 35.7 3.7 9.972 2.7
0.2 5 0.05 20 4 9.95 2.49
0.4 2.5 0.1 10 4.4 9.9 2.25
0.6 1.67 0.15 6.67 4.75 9.85 2.075
0.7 1.43 0.176 5.68 4.9 9.824 2.005
0.8 1.25 0.8 1.25 4.5 9.2 2.045
0.84 1.19 9 0.11 0.5 — —
 Chart Title 1/YxsM 2.0381
3.5 YxsM 0.49065306 gX/gS c)
ms 0.0578 g S/g X-h d)
3 f(x) = 0.0577894774299668 x + 2.03807290993656
R² = 0.936734117717279 kd 0.02835975 h-1
2.5

2
1/Yxsap

1.5

0.5

0
0 5 10 15 20 25
1/D

Chart Title
14
f(x) = 0.147622881288507 x + 1.07115948255481
12 R² = 0.974310759914176
DS/X·D 1/(D+kd)
0.156 12.7616543 10
0.27 7.79060433
0.498 4.37905548 8
1/(D+ks)

0.9 2.33448639
6
1.245 1.59144504
1.405 1.37294792 4
1.635 1.20720497
— 1.15159645 2

0
0 10 20 30 40 50 60 70 80 90
1/S
1/um 1.0712
um 0.93353249 b)
Ks/um 0.1476
Ks 0.1377894 a)
 S0
YMxs
ms
Kd

um
Ks

Chart Title
3.5

3
f(x) = 0.0774214574480984 x
R² = 0.9936138224947
2.5
D, h-1 S, mg/L X, mg/L 1/D Yap 1/yap
0.05 15 162 20 0.33402062 2.99382716 2
0.11 25 210 9.09090909 0.44210526 2.26190476
0.24 50 250 4.16666667 0.55555556 1.8 1.5
0.39 100 235 2.56410256 0.5875 1.70212766
0.52 140 220 1.92307692 0.61111111 1.63636364 1
0.7 180 205 1.42857143 0.640625 1.56097561
0.82 240 170 1.2195122 0.65384615 1.52941176 0.5

0
0 5 10 15
 500 mg/L
0.67585834
0.0774 h-1
0.05231144 h-1

1.43020595 h-1 Considerando el efecto del metabolismo endógeno

195.122998 mg/L

Chart Title Chart Title

3.5 12

3
f(x) = 0.0774214574480984 x + 1.47961973387674 10
R² = 0.9936138224947 f(x) = 136.426052911742 x + 0.699159954019367
2.5 R² = 0.998756531987954
8
1/(D+kd) 1/S
2 9.77407848 0.06666667
6.16099535 0.04 6
1.5 3.42100882 0.02
2.26085043 0.01 4
1 1.7473004 0.00714286
1.32923674 0.00555556
0.5 2
1.14637956 0.00416667

0 0
0 5 10 15 20 25 0 0.01 0.02 0.03 0.04 0.05 0.06 0.07 0.08
metabolismo endógeno

itle

x + 0.699159954019367

0.05 0.06 0.07 0.08