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Genetic polymorphism of glutenin subunits with high molecular weight in

grain Triticum aestivum L., Triticum spelta L. and Triticum durum DESF.

Conference Paper · February 2021

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6 authors, including:

Milan Chňapek Želmíra Balážová

Slovak University of Agriculture in Nitra - Slovenska posnohospodarska univerzita… Slovak University of Agriculture in Nitra - Slovenska posnohospodarska univerzita…
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Dana Rajnincová Janka Drábeková

Slovak University of Agriculture in Nitra - Slovenska posnohospodarska univerzita… Slovak University of Agriculture in Nitra - Slovenska posnohospodarska univerzita…
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 Biotechnology & Biotechnological Equipment

ISSN: (Print) (Online) Journal homepage: https://www.tandfonline.com/loi/tbeq20

Poster Abstracts

To cite this article: (2021) Poster Abstracts, Biotechnology & Biotechnological Equipment,
35:sup1, S62-S128, DOI: 10.1080/13102818.2020.1871545

To link to this article: https://doi.org/10.1080/13102818.2020.1871545

© 2021 The Author(s). Published by Informa

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Published online: 26 Feb 2021.

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Antibacterial activity of aqueous solutions of
essential oils as an indicator for their content
Nelly Georgieva , Nevena Lazarova Zdravkova,
Yoana Todorova, Denitsa Tsanova and
Dimitar Peshev
Department of Biotechnology, University of Chemical
Technology and Metallurgy, Sofia, Bulgaria
Department of Chemical Engineering, University of
Chemical Technology and Metallurgy, Sofia, Bulgaria
email address: nelly.georgieva@yahoo.com.
According to market forecasts, annual consumption of
essential oils on the world market is growing steadily,
reaching 403 thousand tonnes in 2025. The steam distilla-
tion of the plant materials to produce essential oils gener-
ates vast amounts of aqueous fractions as by-products.
Certain amounts of essential oils remain dissolved in these
fractions, predetermining their biological activity. If dis-
carded into the environment they have a negative impact
on the ecological balance. At the same time, these aque-
ous fractions are an attractive source of high value-added
substances if subjected to valorization. A preliminary
appraisal of their quality is required prior to both disposal
and valorization. In the present work, antibacterial activity
is suggested as an indicator of the content of essential oils
in the analyzed products, as far as they are known for their
bactericidal potential. To prove the concept, antibacterial
activity of pure essential oils dissolved in water in different
concentrations was determined using Gram-negative
Escherichia coli K12 and Gram-positive Bacillus subtilis 356
bacterial strains. The minimum inhibitory concentrations
of essential oils and antibacterial activity using the agar-
diffusion method were determined.
Funding: This work was supported by the Bulgarian
National Science Fund (contract KP-06-H37/14).
Characterization of Romanian honeys
according to their physicochemical parameters
and FTIR spectroscopy
Giorgiana Valentina Blaga(costea) , Elena Enachi,
Andreea Veronica Dediu, Rodica Mihaela Dinica ,
Vasilica Barbu and Camelia Vizireanu
rea
Faculty of Food Science and Engineering, “Duna
de Jos” University, Galati, Romania
Faculty of Science and Environment, “Duna rea de
Jos” University, Galati, Romania
email address: giorgiana.blaga@ugal.ro. The aim of
this study was to characterize monofloral and multifloral
honey samples through various physicochemical
BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT S79
parameters. For this purpose, eighteen honey samples
were collected directly from beekeepers in 2015 from
different Romanian regions and with different botanical
origins. Microscopic analysis of the pollen in the honey
was used to determine its botanical origin.
Physicochemical analysis included the determination of
pH, electrical conductivity, moisture, ash and colour
parameters: L, a, b. The FTIR-ATR analysis of the
honey samples allowed the identification of several
bands characteristic to different organic compounds
functional groups present in these samples. The
obtained results showed considerable differences in the
physicochemical parameters, between honeydew and
blossom honeys. Concerning the physicochemical
parameters, all the samples met the EU Council Directive
2001/110 for all the parameters.
Funding: The authors are grateful for the technical sup-
port offered by Center MoRAS developed through Grant
POSCCE ID 1815, cod SMIS 48745 (www.moras.ugal.ro).
Genetic polymorphism of glutenin subunits
with high-molecular weight in grain Triticum
aestivum L., Triticum spelta L. and Triticum
durum DESF

Milan Chn  apek, Zelmira Balazova
, Andrea

Spalekova , Dana Rajnincova , Janka
bekova
Dra and Zdenka Ga lova 
Department of Biochemistry and Biotechnology,
Faculty of Biotechnology and Food Sciences, Slovak
University of Agriculture, Nitra, Slovakia
Department of Mathematics, Faculty of Economics
and Management, Slovak University of Agriculture,
Nitra, Slovakia
email address: zdenka.galova@uniag.sk. The collec-
tion of 45 genotypes of bread wheat (Triticum aestivum
L.), 33 genotypes of spelt wheat (Triticum spelta L.) and
18 genotypes of durum wheat (Triticum durum DESF.)
were analyzed by SDS-PAGE. Variability of electrophor-
etic spectra of storage proteins was monitored and
based on this a dendrogram of relationships was pre-
pared. Bread wheat genotypes were found to be homo-
genous and nine electrophoretical profiles were
detected. Bread wheat genotype with component com-
position of HMW-GS 0, 7 þ 9, 5 þ 10 was the most fre-
quent (78%) of all genotypes. The genotypes Magvas, SK
76, Favorit and Astela revealed the highest Glu-score
value (9). Secalin block – Gld 1B3, a marker for resistance
against wheat rust and also a marker for worse techno-
logical quality was observed in gliadin spectra of 12 clas-
sified bread wheat genotypes. Genotypes of spelt wheat
were characterized as a multiline and 43 different
 S80 POSTER ABSTRACTS
individual HMW-GS were identified. The highest Glu-
score value (8) achieved 10 genotypes. However, eval-
uated durum wheat genotypes had lower Glu-score
value (2) than other wheat cultivars, which confirmed
their applicability for pasta production. We have con-
structed dendrogram of relationships on the basis of the
storage proteins, which divided collection of bread
wheat into two clasters and four subclasters.
Funding: This work was co-funded by European
Community under project no. 26220220180: Building
Research Centre “AgroBioTech", by grant VEGA No.1/
0246/18 and KEGA project No. 025SPU-4/2018.
Laboratory-based high-resolution X-ray
tomography for three-dimensional visualizing
the natural diatoms
€rgen Gluch and Ehrenfried Zschech
Qiong Li, Ju
Fraunhofer Institute for Ceramic Technologies and
Systems IKTS, Dresden, Germany
email address: qiong.li@ikts.fraunhofer.de.
Biomaterials, with natural features of hierarchical organ-
ization and adaptability, inspire the design of more reli-
able, efficient, biocompatible and environmentally
friendly materials. Therefore, it is very important to
understand the hierarchical structure of these natural
biomaterials. Here, an advanced technique of laboratory-
based high-resolution transmission X-ray microscopy
(TXM) is used to investigate the whole diatoms without
prior staining. The major advantage of this technique for
biological specimens is providing three-dimensional (3D)
information without extensive sample preparation proce-
dures. Diatom frustules and natural diatoms were non-
destructively imaged by TXM. The detailed 3D structural
information of diatoms imaged non-invasively demon-
strates the excellent capabilities to study complicated
hierarchical biological structures by TXM. Furthermore, a
sample holder set-up is described for the TXM system to
visualize the diatoms three dimensionally in quasi-living
state allowing further promising studies of living species.
Evaluating the diversity and cellulolytic
potential of Black Sea shipworm (Teredo
navalis) gill symbionts
, Ileana
Irina Lascu, Maria Preda, Ioana Mereuīa
nase
Stoica, Iulia Chiciudean and Ana Maria Ta
Department of Genetics, Faculty of Biology,
University of Bucharest, Bucharest, Romania
email address: irina.lascu@drd.unibuc.ro. In order for
biocatalytic cellulose depolymerization to gain footing
View publication stats
in the biofuel and biosynthesis industries, the cellulo-
lytic enzymes used must be efficient enough to make
these processes economically viable. Such enzymes
are likely to be produced by microorganisms from
specialized environments, in this case, tissues of the
xylotrophic marine bivalve Teredo navalis. Symbiotic
bacteria located in the shipworms’ gills secrete various
cellulases, enabling the digestion of ingested wood
particles. In this study, gill tissue from T. navalis speci-
mens collected from the Romanian shore of the Black
Sea was used for a cellulose-enriched culture, total
DNA extraction and pure strain isolation. The ship-
worm specimens’ phylogeny/phylogeography was
investigated via cytochrome-oxidase subunit I (cox1)
gene sequence. DNA metabarcoding of the cellulose-
enriched culture gave a detailed inventory of symbiont
diversity. Pure isolates were tested for their ability to
produce endoglucanase (EC 3.2.1.4) through Congo
Red staining and DNS assays, with enzyme activity
tested at various temperatures. Cellulase-producing
strains were taxonomically classified by 16S rRNA gene
sequencing. Our results have great potential to be
employed in biorefinery applications.
Funding: This work was supported by the
Romanian National Authority for Scientific Research
and Innovation, CCCDI – UEFISCDI, project number 1/
2018, ERANET-Marine Biotechnology METAMINE, within
PNCDI III.
Variation of total phenolic content, organic
acids, antiradical and antimicrobial activity in
callus cultures of highbush blueberry
(Vaccinium corymbosum)
Dora Livkisa , Anna Ramata Stunda, Valda
Valkovska, Ma rtiņs Borodusķis, Elza Kaktiņa,
Baiba Silamiķele, Anete Borodusķe and
Nils Rostoks
Faculty of Biology, University of Latvia, Riga, Latvia
Faculty of Chemistry, University of Latvia,
Riga, Latvia
email address: dora.livkisa@gmail.com. Known for
its antioxidant activity, blueberry (Vaccinium corymbo-
sum) is becoming more popular for consumption and
various applications in medicine and nutrition. Tissue
cultures, genetic engineering and other biotechno-
logical solutions allow to overcome the availability lim-
itations set by plant seasonality. In this study, we
looked at the differences in total phenolic content
(TPC), antiradical activity (ARA), antimicrobial activity
and quantity of selected organic acids in leaves of