Think Pair Share Assignment Chap 2 part 1

Members;

1) Aishah Nur Damia binti Roslan (A20SC0013)

2) Nur Farah Syuhada binti Mohamad Aziz (A20SC0237)

Answers

1) Leuconostoc mesenteroides is a fastidious microbial species because it has complex nutritional

requirements. It's difficult to cultivate in the lab due to their complex or limited nutritional and/or
environmental requirements. Leuconostoc mesenteroides will only survive in the presence of
specified nutrients in the culture medium. Leuconostoc mesenteroides is a facultative anaerobe
that requires many growth hormones and amino acids (alanine, arginine, asparagine, etc) to grow.
That is why Leuconostoc mesenteroides has more requirements for culture media than
Escherichia coli.

2) Leuconostoc mesenteroides is a limited biosynthetic capacity microbial. The nutritional needs of

L. mesenteroides can be satisfied by preparing either a highly supplemented defined medium, a
rather laborious under- taking because of all the individual nutrients that need to be added, or by
preparing a complex medium, a much less demanding operation.

3) Because it would consume a lot of time to create a defined medium, just using a complex medium
which already has a bunch of nutrients for the bacteria to grow on.

4) Complex medium is most likely where e-coli will grow faster. Complex medium is a composition
of media that is not known. Whereas defined media is synthesized by adding a calculated
component, so its composition is known. E-coli will grow faster in complex mediums because it
consists of yeast extract and peptone, which are complex hydrolyses and provide ready-made
compounds like amino acids. The defined medium uses simple ingredients and has to prepare
every biomolecule that e-coli needs to grow. Hence, e-coli will grow faster in complex medium
than in defined medium.

5) The major macronutrients used in culture media are Carbon, Nitrogen, Oxygen, Hydrogen,
Potassium, Phosphorus, Calcium, Magnesium and Sulphur. Carbon is used as material to make
new cell. Nitrogen is important for amino acids components, nucleic acid and proteins.
Phosphorus is a key element in nucleic acids and phospholipids. Sulphur is present in the amino
acids cysteine and methionine and also in several vitamins. Potassium is required for the activity
of several enzymes. Magnesium is to stabilize ribosomes, membranes, and nucleic acids and also
 for activity of many enzymes. Calcium is not required by all cells but can help to stabilize
microbial cell walls, and to stabilize the heat of endospores.

6) Growth factors are vitamins, amino acids, purines, or various other organic molecules.
Micronutrients involved in this culture growth is Ferum which plays a major role in cellular
respiration. Other than that, manganese plays similar role to iron. Micronutrients play a role as
cofactors for enzymes.

7) Culture media that supply the nutritional needs of microorganisms are either defined or complex.
Many microorganisms can be grown in the laboratory in liquid or solid culture media that contain
the nutrients they require. Pure cultures of microorganisms can be cultured and maintained if
aseptic technique is practiced.