Suppose axon A enters a ganglion (a cluster of neurons) and axon B leaves on the

other side. An experimenter who stimulates A can shortly thereafter record an impulse
traveling down B. We want to know whether B is just an extension of axon A or whether
A formed an excitatory synapse on some neuron in the ganglion, whose axon is axon B.
How could an experimenter determine the answer? You should be able to think of more
than one good method. Presume that the anatomy within the ganglion is so complex
that you cannot simply trace the course of an axon through it.

Answer: To determine whether the path is the same or not, one method would be to
test the alleged link in reverse. To add another dimension, shoot from both sides and at
various intensities to check for consistency. If properly assessed, this would indicate
whether one component or several parts are at play in this situation. It would be obvious
if they were two separate components joined or if they were all one piece based on how
each passage went back and forth.
 Ganglion neuron is cell body that has an axon that is grow out of ganglion. Axons
are growing out of ganglion that is associated with sensory endings.
 There is more than one good method that determines whether B is just an
extension of axon A or whether A formed an excitatory synapse on some neuron
in the ganglion, whose axon is B.
 Compare the velocity of transmission from point A to point B with velocity of A
and B alone. If the velocity in either A one alone is faster than velocity from A to
B is then it shift that there is synapse between A and B which means they are
axons of different neurons and if velocity is similar, then it shows that B is an
extension of A.
 By using antidromic conduction, if by stimulating B antidromic impulse produces
in A. It shows that A and B are present on same axon.
 Cut either axon A or B will result in degeneration of opposite axon. Then these
two are present on same axon by cutting one axon will harm another and if not
harm, then they present on separate axons.
 By injecting a dye into axon A or axon B will result in spreading of dye on the
other then they may be the same axon but few dyes can across synapses also. If
the dye does not invade another then they may be on separate axons.
 By injection of magnesium into the ganglion prevents calcium to enter the
presynaptic terminal. This blocks the synaptic transmission which means A stops
finding B. This means they are on separate axons.