Cortez, Faith Ann 10/3/22

BSMT 2 Prof. Cecilio Ichon, RMT

Activity 4:
Carbohydrates
I. Overview:
Carbohydrates, or carbs, are sugar molecules. Along with proteins and fats,
carbohydrates are one of three main nutrients found in foods and drinks. Your
body breaks down carbohydrates into glucose. Glucose, or blood sugar, is the
main source of energy for your body's cells, tissues, and organs.
II. Objectives:
1. Define the meaning of Carbohydrates Test like Molisch Test and
Benedict’s Test.
2. To determine the presence or absence of reducing sugar in the solution
3. To determine the glucose concentration in the solution quantitatively.
III. Materials:
Google, Cellphone, Internet, You Tube
IV. Drawing:
V. Questions and Answers:
1. What is the Color due to Molisch test for carbohydrates?
- In this test, concentrated sulfuric acid converts the given carbohydrate into
furfural or its derivatives, which react with α-naphthol to form a purple colored
product. The appearance of purple or violet ring confirms the presence of
carbohydrate.
2. What is carbohydrate in biochemistry?
- Carbohydrate is a group of organic compounds occurring in living tissues and
foods in the form of starch, cellulose, and sugars.
3. How are carbohydrates tested in biochemistry?
- Carbohydrates. Starch is detected using iodine solution. This turns blue-black
in the presence of starch. Reducing sugars are detected using Benedict's
solution.
4. Why is Benedict's test important?
- The primary application of Benedict's test is to detect the presence of simple
carbohydrates in an unidentified analyte. This test can be used to check for
reducing sugars that hold free aldehyde or ketone functional groups. The
reducing sugar can be either a monosaccharide or a disaccharide.

VI. Reference:
https://byjus.com/chemistry/tests-of-carbohydrates/#:~:text=Take%202ml%20of
%20given%20sample,presence%20of%20carbohydrate%20is%20confirmed.

FAITH
ANN CORTEZ
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