BIRLA INSTITUTE OF TECHNOLOGY AND SCIENCE, PILANI, GOA

Date: 1.5.2018 Genetics (BIO F 243) Time : 90 min

Closed Book Comprehensive examination Max marks: 20

Q1. In the laboratory you created a lambda mutant using mustard gas. You further infected an E.coli cell
with this mutated lambda; Upon infection , you realized that your E.coli cell was mutated for Hfla .Which
developmental pathway will lambda follow in this case? Which pathway would have been followed on a
wild type E.coli host? (2x2.5 M)

Q2. Explain at least three strategies to clone a PCR product into a cloning vector? (3M)

Q3. How does CI cooperativity induce lysogeny in lambda? (3M)

Q4. pMB1 is digested with BamHI and EcoRI. The digestion pattern is as follows:

BamHI EcoRI BamHI +EcoRI

13 26 7 Map the plasmid.

6 6
(2M)
4 4
Q5) How does EcoRI
3 3 endonuclease recognize the
sequence it cleaves? What are
the symmetry relationships in the
interaction between the enzyme
and the substrate? How many sites of EcoR1 you will expect in the sequence of DNA which is 20Kb.
(3M)

Q6) Why H3 and H4 histones are more conserved compared to H 2A and H2B? Comment (2M)

Q7) Briefly explain the principle of interrupted mating experiment? (2M)