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Synthesis of Nanogels of PNIPAM-Chitosan

Article  in  Microscopy and Microanalysis · July 2020

DOI: 10.1017/S1431927620021005

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 2262 Microsc. Microanal. 26 (Suppl 2), 2020
doi:10.1017/S1431927620021005 © Microscopy Society of America 2020

Synthesis of Nanogels of PNIPAM-Chitosan

Eleazar León, Pedro-A. Hernandez-A., Nadia. Garcia-F., Hiram Higuera-V., lucia-E. Grijalva-C., Jorge-
L. Iriqui-R. and Mario Alvarez-R.
Universidad Estatal de Sonora, Hermosillo, Sonora, Mexico
In this work, novel nanogels sensitive to the temperature were synthesized to depart from PNIPAM, to
establish a liberation control according to the temperature of the way. It was characterized by means of
DLS (size), AFM (morphology), Potential Z (charge), Microscope of fluorescence (internalization,see
figure 1) and tests of cellular viability were realized. These results verify the effects of the structure of a
polymer hidrofílico in the LCST of the PNIPAM.[1]
The poli (N-isopropilacrilamida) (PNIPAM) expresses a LCST that corresponds to temperature between
30 and 35 ºC induced entropically. The derivatives of the PNIPAM present this behavior of its LCST that
structurally depends on the hydrophobicohydrophilic equilibrium of of their polymer chains, according to
the number of water molecules joined by means of the bond of hydrogen.
We will use N,N′-Methylenebis(acrylamide) as a cross-linking agent between ANIPAM and Chitosane.
The presence of stabilizing agents to control the size of the hydrogels, where 200 nm structures were
obtained, as shown in Figure 2, is important.
increasing the temperature of the aqueous medium will result in the conformational transition of the
structure of the PNIPAM to achieve a favorable balance to only hydrophobic segments, so water molecules
have to spread to the outside of the polymeric nucleous, which by means of the Z potential, a data of 0,1
was obtained.[2]
The synthesis of the nanogels will be carried out in accordance with the following procedure:
In a 3-hole flask, with exits and controller flux of teflon, 195 mg dissolves of the monomer NIPAM in
100 ml of water ultra pure. We will maintain magnetic agitation during the process. After dissolving
completely, 1.95 mm are added of N,N’-metylenbisacrisamide (ENCORE) and 10 of dodecyl sulfate of
sodium (SDS), the constant agitation is supported up to dissolving completely all the components of the
solution.
Next step, it is realized purged directly in the solution to displace the dissolved oxygen, this procedure of
purged is supported by means of one hour to constant pressure. Finally, the reaction temperature increases
up to 70 ºC, later 2.20 mm of persulfate of ammonium are added to the solution of reaction, the
temperature, the agitation and the current of nitrogen stay constant for period of 2 hours. The sample will
be she will submit to a process of centrifuged 14000 rpm in 15 minutes to separates of nanogel.

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 Microsc. Microanal. 26 (Suppl 2), 2020 2263

Figure 1. Epithelial cells, A( Membrane), B(nucleus). Cancer cells, C(Membrane), D(Nucleus).

Liberation of Np's

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 2264 Microsc. Microanal. 26 (Suppl 2), 2020

Figure 2. Topography image of PNIPAM's AFM, where we can observe the morphology of the structures
of the nanogels, we can observe a uniform distribution of size.

References
[1] Esquivel, R., Canale, I., Ramirez, M., Hernández, P., Zavala-Rivera, P., Álvarez-Ramos, E., & Lucero-
Acuña, A. (2018). Poly(N-isopropylacrylamide)-coated gold nanorods mediated by thiolated chitosan
layer: thermo-pH responsiveness and optical properties. e-Polymers, 18(2), 163–174.
https://doi.org/10.1515/epoly-2017-0135.
[2] Hernández, P., Lucero-Acuña, A., Moreno-Cortez, I. E., Esquivel, R., & Álvarez-Ramos, E. (2020).
Thermo-Magnetic Properties of Fe3O4@Poly(N-Isopropylacrylamide) Core–Shell Nanoparticles and
Their Cytotoxic Effects on HeLa and MDA-MB-231 Cell Lines. Journal of Nanoscience and
Nanotechnology, 20(4), 2063–2071. https://doi.org/10.1166/jnn.2020.17324

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