Service Manual V1.

MAGLUMI 600 Chemiluminescence Analyzer

Shenzhen New Industries Biomedical Engineering Co., Ltd

 Contents
1、Whole analyzer .......................................................... 1
1.1 Power supply of the whole analyzer ..................................... 1
1.1.1 Sketch map of Maglumi 600’s power supply network . 1
1.1.2 Trouble shooting of the power supply ......................... 1
1.2 Temperature of the analyzer ................................................. 4
1.3 Liquid level detection ............................................................ 5
1.4 Check the grounded status of the analyzer .......................... 7
1.5 Examples of all the kind of Soldering joint ............................ 7

2、Component 01-COP ................................................... 9

2.1 M600-01-E00-COP layout and functioning ........................... 9
2.1.1 Layout of M600-01-E00 .............................................. 9
2.1.2 Function of the M600-01-E00 ..................................... 9
2.1.3 M600-01-E00 electronic component list ..................... 9
2.2 Possible problems and solutions for COP .......................... 10

3 Component 02 - Sample & Reagent .......................... 11

3.1 M600-02-E00-Sample & Reagent Layout and Functioning 11
3.1.1 Layout of M600-02-E00-Sample&Reagent ............... 11
3.1.2 M600-02-E00 PCB Functioning ................................ 11
3.1.3 M600-02-E00-Sample&Reagent .............................. 12
3.2 Functions description of pipettor’s interface PCBs ............. 13
3.2.1 M600-02-E01 PCB function description ................... 13
3.2.2 M600-02-E02 PCB function description ................... 13
3.2.3 M600-02-E03 PCB function description ................... 13
3.2.4 M600-02-E04 PCB function description ................... 13
3.3 Possible problems and solutions for pipettor ...................... 14
3.3.1 Inner pipetting arm possible problems...................... 14
3.3.2 Outer pipetting arm possible problems ..................... 15
3.3.3 Pipetting arm vertical direction common problems ... 16
3.3.4 Pipetting possible problems and solutions................ 17
3.3.5 sample/reagent area troubleshooting ....................... 18
3.3.6 Common troubleshooting for pipetting Pump ........... 21

4. Component 03-incubator &Wash station &Pusher . 23

4.1 M600-03-E00-Washer PCB layout and function ................. 23
4.1.1 M600-03-E00 PCB ................................................... 23
4.1.2 M600-03-E00 PCB functional description ................. 23
4.1.3 M600-03-E00 specification ....................................... 24
4.2 Wash station function description ....................................... 25
4.2.1 Wash station interface function................................. 25
 4.2.2 Incubator Interface PCB function description ........... 26
4.2.3 Loader Interface PCB Function Description ............. 27
4.2.4 Pusher Interface PCB function description ............... 27
4.3 Failure diagnosis ................................................................ 28
4.3.1 Washer station common problems and diagnosis .... 28
4.3.2 Possible problems and solution for Incubator ........... 32
4.3.3 Possible problems and solution for Pusher .............. 34
4.3.4 Possible problem and solution for Loader ................ 35

5、Component 04-chamber.......................................... 37
5.1 layout of M600-04-E00-Chamber PCB and function........... 37
5.1.1 M600-04-E00 PCB ................................................... 37
5.1.2 M600-04-E00 function describing ............................. 37
5.1.3 M600-04-E00 electronic components ....................... 37
5.2 Chamber function and trouble shooting .............................. 39
5.2.1 Chamber interfaces function description .................. 39
5.2.2 Possible problems and solution for Chamber ........... 40
5.2.3 Chamber pumps trouble shooting ............................ 42
5.2.4 PMT trouble shooting ............................................... 44
1、Whole analyzer

1.1 Power supply of the whole analyzer

1.1.1 Sketch map of Maglumi 600’s power supply network

Figure 1.1 Maglumi 600 power supply network

1.1.2 Trouble shooting of the power supply

（1）Analyzer power supply is suddenly off

Possible reasons：
1）Melt of the Fuse
2）Damage of the switching power supply
3）Bad connection of the power supply
4）Short circuit between the power supply network and the grounded
Solutions：
1） Check whether there is a short circuit in the power supply, or between
the power supply and machine metal shell.
2） Disconnect the cable between the switching power supply and
M600-01-E01 board, Sample & Reagent Area. Switch on the main
switch of the analyzer, then use the multi-meter to test whether there is
+24V output of the switching power supply.

1
 Figure 1.2 The DC voltage output of the switching power supply

3) If the switching power supply have +24V output, then pull out the
cables between M600-01-E01 and other main board one by one, and
check which PCB board is short circuit.

Figure1.3 M600-01-E01

4) If there is no output of the switching power supply, then check the input
terminal whether there is 220V or not.
5）If the switching power supply input voltage is 220V, but there is no 24V
output, that means the switching power supply is damage.

2
 6）If there is no 220V input voltage, check the two fuses inside the main
switch and the cable from the main switch to the switching power
supply.

Figure 1.4：Fuse of the main switch 250V/6A

（2）Deputy switch unable to control the power supply of each main boards
Possible reasons：
1）Damage of the deputy switch
2）Damage of the cable which connecting to the deputy switch
Solutions:
1） Check the soldered dot of the red and blue wires
2） If the connection is OK, then the problem come from the deputy switch

Figure 1.5 Deputy Switch

（3）Voltage alarm in User software, or not all the LED on M600-04-E00

PCB are on.
Possible reasons：
1）voltage of the 04-E00 PCB is unstable or the PCB is damage.
2）Wrong indicating of the software.
Solutions：

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 1） Test the voltage of the +5V、-15V、-15V testing holes on PCB 04-E00

Figure 1.6 M600-04-E00 testing hole

2） Normal situation, if the value of the +15V、+5V、-15V、-15V testing
holes have a deviation more than 0.1V please adjust the blue
potentiometer to make the deviation less than 0.1V.
3）If the voltages are normal, enter the Service software, then from
“Globals”-“Errorcard”-“Calc.Temp”-“Writeparam”, to save the
parameters when the PMT is loaded.

1.2 Temperature of the analyzer

The heating module and cooling module are circuit loops which are
composed of power supply switch, sensor and temperature control device. The
sensor is used to check whether the temperature is normal. Usually one
centigrade is equal to 10 mV output voltage of the sensor.

Figure 1.7 Temperature control Schematic

Common troubles：
1）Incubator heating abnormal, or the cooling function of the sample &
reagent area is abnormal.
2）Temperature alarm in user software.
Solutions：
1） Heating function of the incubator is abnormal. Check whether the LED

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 D1 of the 03-E05 PCB is on, Test whether the voltage of the
temp-sensor signal wire (TP2 test hole) is normal; if it’s normal, then
check whether the cable of the temperature sensor is fixed; if it’s fixed,
check the resistance between the J6 heating metal, the resistance is
24±4Ω normally. If the resistance is damage, the multi-meter shows no
resistance; if all above are OK, then change the 03-E05 board.
2） Cooling function of the reagent area is abnormal. Check whether the
fans under the reagent area work normally, if not, check the soldering
cables or change the fans (The temperature will be too high to melt the
fuse without the fans). Test whether the temp-sensor signal wire (J4
yellow signal wire) is normal. If all above are OK, change the -02-E04
PCB.

1.3 Liquid level detection

The liquid levels of the starters are detected by capacitance method. And
the system liquid and waste levels are detected by reed switch.

Figure 1.8 M600-01-E02 LLD board

Normally, when the starter 1 (starter 2) is enough, the LED D5 (D6) is on.
When the system liquid is enough, the LED D7 is off. And the LED D8 is off
when the waste liquid is not full.
Common trouble：
1） The starters’ liquid level couldn’t be detected correctly, and alarm even
the starts are enough.
2） The system liquid and waste liquid alarm abnormally.
Possible reasons：
1） The sensitivity of the starters’ liquid level detection is not adjusted
properly.
2） Damage of the liquid level detection PCB M600-01-E02.
3） The detecting stick of the starter is oxidation or the rubber O-ring is
missing
4） Bad contact of the plate metal which located at the bottom of the
starters.

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 5） Damage of the reed switch.
Solutions：
1） Check whether the detecting stick is clean; Check whether the
rubber o-ring is missing; Check whether the plate metal under the
starters is connected with the analyzer’s shell correctly; Check
whether there’re liquid drops on the M600-01-E02 PCB

O ring

Figure 1.9 Rubber O-ring of the starters’ detecting stick

2）Adjust the blue potentiometers on the M600-01-E02 PCB to change the
sensitivity of the starters’ liquid level detection (there’re LEDs to show
the status). Adjust the potentiometer counter-clockwise will increase the
sensitivity.
3）Use the multi-meter’s short-circuit function to check whether the reed
switch works normally. Use the two head of the multi-meter to touch the
outside and inside of the metal joint. Normally, move the magnet-ring to
some certain position, the multi-meter will show the status of
short-circuit.

信号接头
LLD connector

短路档
Short-circute function

Reed
磁簧开关Switch

Figure 1.10 checking the reed switch

4）If all above are normal, change the 01-E02 PCB.

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1.4 Check the grounded status of the analyzer

（1）If the power supply is grounded, choose the short-circuit function of the
multi-meter. Use the two heads of the multi-meter to touch the analyzer
(metal shell or the metal screw) and the ground wire interface of the
multi-socket respectively. If the multi-meter alarm and beep continuously
(the resistance is 0), it means that the analyzer is connected good with
the multi-socket properly

Figure 1.11 Short-circuit function of multi-meter

（2）Use the two heads of the multi-meter to touch the earth line interface and
the null line interface, the voltage should be less than 2V if proper
grounded.

1.5 Examples of all the kind of Soldering joint

（1）Soldering joint of Motor

Figure 1.12
（2）Soldering joint of Sensor

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 Figure 1.13
（3）Soldering joint of Encoder

Figure 1.14
（4）Soldering joint of heating metal

Figure 1.15

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2、Component 01-COP

2.1 M600-01-E00-COP layout and functioning

2.1.1 Layout of M600-01-E00

Figure 2.1 M600-01-E00 PCB board

2.1.2 Function of the M600-01-E00

（1）Control of the temperature in the heating and cooling area.

（2）Liquid level signals of the starters, system liquid and waste liquid
（3）Store the commands of the software, and the components moving control

2.1.3 M600-01-E00 electronic component list

ID
Type Explanatory Notes Remarks
number
D4 Indication light of communication between Green
the 485 BUS line and COP sending end,
LED normally appears as twinkle
D5 Indication light of communication between Blue
the 485 BUS line and COP receiving end,
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 normally appears as twinkle
D3 Indication light of power supply(voltage
yellow
3.3V)
D2 Indication light of power supply(voltage
yellow
5V)
D1 Indication light of power supply(voltage
yellow
24V)
D6D7 Attended to be used by technician for Blue
system development
Fuse F1 24V Fuse 5A
Jumper JP2 Please insert JP2 and JP3 when proceed
JP3 program upgrading for processor and
remove them after update finished(insert
to JP1 and JP4)

Figure 2.2 M600-01-E00 Jumpers

2.2 Possible problems and solutions for COP

（1）Communication problem between software and analyzer

Possible reasons：
1）USB-RS232 cable is loose
2）COM Port setting in software have been changed
3）M600-01-E00 is damaged
4）JP2 and JP3 jumpers on M600-01-E00 are not removed
5）The power supply is unstable or there is interference source nearby.
Solutions:
1） Check the USB-RS232 cable, whether the connector is loose or
damaged, change it if it is damaged.
2） Check whether the COM Port setting in software has been changed.
Normally the default communication port is COM 1.
3） Check the Jumpers. If the JP2 and JP3 are not removed, some
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 computer may have some problems that the COM port is closed after once
the computer is in sleep mode.
4）If all above are OK, but the firmware can’t be upgraded or the computer
is unable to connect to the software, then replace the M600-01-E00
PCB board.

3 Component 02 - Sample & Reagent

3.1 M600-02-E00-Sample & Reagent Layout and Functioning

3.1.1 Layout of M600-02-E00-Sample&Reagent

Figure 3.1 M600-02-E00 PCB component view

3.1.2 M600-02-E00 PCB Functioning

（1）Control of the vertical, inner and outer motor drivers

（2）Control of the bypass pump
（3）Control of the dilutor
（4）Control of the waste pump of pipettor
（5）Control of the receiving process of liquid level detection signal
（6）Sample and reagent track detection
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（7）Scan the sample track barcode
（8）Read reagent information from RFID

3.1.3 M600-02-E00-Sample&Reagent

Type ID Explanation Remarks

D12 Indicating light and signal testing hole for the sensor of green
TP12 the plunger pump (dilutor).
After initialize the plunger pump, the plunger reaches
the top, and the sensor is not blocked, at this time the
D12 LED is on, and the test voltage at TP12 is below
300mV;
The sensor is blocked when the plunger is at other
positions, the D12 LED is off, and the test voltage is
above 4.5V

D13 Indicating light and signal testing hole for the sensor of green
TP13 shake speed monitoring.
When the sensor of shaker motor is not blocker, the D13
LED is on, the voltage at TP13 is below 300mV;
When the sensor is blocked by sheet metal, the D13 is
off, and the voltage at TP13 is above 4.5V;

D14 Sensor LED for inner pipetting arm initialization and green
LED TP9 hole for signal test.
When the sensor is not blocked by the sheet metal on
the inner arm, the D14 LED is on, and the voltage at
TP9 is below 300mV;
When the sensor is blocked, the D14 LED is off, and the
voltage at TP9 is above 4.5V

D15 Sensor LED for pipettor’s vertical initialization and hole green
TP11 for signal test.
When the sensor is not blocked by the vertical sheet
metal, the D15 LED is on, and the voltage at TP11 is
below 300mV;
When the sensor is blocked, the D15 LED is off, and the
voltage at TP11 is above 4.5V

D16 Sensor LED for outer pipetting arm initialization and green
TP10 hole for signal test.
When the sensor is not blocked by the sheet metal on
the outer arm, the D16 LED is on, and the voltage at

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 TP10 is below 300mV;
When the sensor is blocked, the D16 LED is off, and the
voltage at TP10 is above 4.5V
D1 LED for +24V power supply yellow
D2 LED for +15V power supply yellow
D3 LED for +5V power supply yellow
D4 LED for +3.3V power supply yellow
D5 Program debugging LED, should only be used by yellow
program developer
Fuse F1 +24Vpower fuse 5A
Upgrade J4 Connector for upgrade the left arm
connector

3.2 Functions description of pipettor’s interface PCBs

3.2.1 M600-02-E01 PCB function description

（1）outer and inner pipetting arm, vertical motor and encoder transfer
（2）liquid level detection signal transfer

3.2.2 M600-02-E02 PCB function description

（1）LLD signal generation

（2）Detection of the outer pipetting arm initialization position by the sensor

3.2.3 M600-02-E03 PCB function description

（1）Pipetting arm plunger pump motor and sensor signal transfer

（2）Pipetting arm wash pump transfer
（3）Pipetting arm waste pump transfer
（4）Pipetting arm washing control valve transfer

3.2.4 M600-02-E04 PCB function description

（1）Detection of the sample and reagent rack insertion

（2）Detection of the sample/reagent area door switch
（3）Shaker motor and speed monitoring transfer
（4）Control the temperature of sample/reagent area

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3.3 Possible problems and solutions for pipettor

3.3.1 Inner pipetting arm possible problems

（1）inner arm unable to initialize

Possible reasons：
1）initialization sensor is damaged
2）M600-02-E00 PCB is damaged
Solutions：
1）If block or unblock the inner arm initialization sensor with a piece of light-proof
sheet, then check the status of D14 whether it changes. If not, replace the sensor.

Figure 3.2 Initialization sensor of inner arm

2）If the sensor is ok, but the motor unable to move and is not locked when doing
inner arm initialization, it suggests that the M600-02-E00 PCB is damaged and
need to replace a new one.

（2）Inner arm unable to move clockwise or anti-clockwise

Possible reasons：
1）The power line does not connect the inner arm motor and encoder well.
2）External resistance.
3）The M600-02-E00 PCB or the motor is damaged.
Solutions：
1）Check the wire which connected to the motor and encoder.
2）Check external resistance by push the inner arm manually

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 3）If the initialization and wire connection are ok, the external resistance does not
exist and the arm is not locked when power on, it suggests the inner arm encoder is
damaged and need to change it together with the motor.

3.3.2 Outer pipetting arm possible problems

Outer arm unable to initialize

Possible reasons：
1） initialization sensor is damaged
2） wire connected to the interface PCB and motor is loosen
3）M600-02-E00 PCB is damaged
Solutions：
1）Check the wire connected to the interface PCB, motor and adaptor.
2）Pull the outer arm to block or unblock the sensor. Normally, the state of the D16
LED will change. Otherwise, the sensor is damaged and you need to change the
M600-02-E00 PCB.

Figure3.3 Initialization sensor of outer arm

3）If the outer arm cannot move when doing initialization and cannot be locked when
power on, it suggests the M600-02-E00 PCB is damaged .

（2）The outer arm cannot move forward or backward

Possible reasons：
1）The power line does not connect the outer arm motor and encoder well.
2）External resistance.
3）The M600-02-E00 PCB or the motor is damaged.
Solutions：
1）Check the wire connected to the motor and encoder.
2）Check external resistance by push the outer arm manually
3）If the initialization and wire connection is ok, the external resistance does not exist
and the arm is not locked when power on, it suggests the outer arm encoder is
damaged and you need to change it together with the motor.
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3.3.3 Pipetting arm vertical direction common problems

（1）Unable to initialize the vertical direction, the probe cannot move up during
initialization
Possible reasons：
1）The probe hits something in the vertical direction
2）The sensor of the vertical direction is damaged
3）The M600-02-E00 PCB is damaged
4）The vertical motor is damaged
Solutions：
1）Adjust the probe to the correct position if it had hit something
2）Check whether the D15 LED changes its status when the sensor is blocked and
unblocked. If not, the sensor is damaged and need to be replaced.

Figure3.4 Initialization sensor of vertical direction

3）If the vertical arm is not locked when power on and cannot move during
initialization, the M600-02-E00 PCB is damaged.

（2）Unable to move upward or downward in vertical direction

Possible reasons：
1）external resistance
2）the motor is damaged
3）bad wire connection
Solutions ：
1）Check the wire of vertical motor.
2）Pull the probe in vertical direction and see if there is stronger resistance at some
position.
2）If initialization is ok, the connection is good and resistance is weak, there is still
error warning, indicating that the motor is damaged.

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3.3.4 Pipetting possible problems and solutions

（1）Liquid level detection fail

Possible reasons：
1）The signal wire of LLD is not well connected
2）The ground wire of the sample/reagent area is not well connected
3）The metallic membrane on the reagent kit is not removed
4）The LLD parameter is not adjusted correct
5）M600-02-E00/ M600-02-E02 PCB is damaged
6）The pipetting probe crashed or rust
Possible reasons：
1） Check the LLD signal wire, and check whether there is liquid or crystal on the
adaptor PCB.

Fig 3.5 conjunction of liquid level detection signal transmission

2）Use multi-meter to check whether the ground wire of sample/reagent area is

connected well to the instrument shell.
3）Use the “Detect” function in service software to check the level detect value.
Normally the difference between maximum and minimum value should be below 3, if it is
more than 3, please check the power supply of the instrument and the grounding or try to
replace M600-02-E02 PCB board.

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 Fig 3.6 “Detect” function in service software
4）If all above are ok, change the sensitivity parameter of LLD gradually(the value is
25 normally),the sensor becomes less sensitive as the value decrease.
5) If the needle is clashed, adjust it back to original position.
6) If the needle is rusty, please check whether there are some rust or crystal material
sticks to the welding point of needle PCB board.

3.3.5 sample/reagent area troubleshooting

(1) Unable to read the sample barcode information which is closest or farthest to
the barcode reader
Possible reasons：
1） Barcode reader installation problem
Solutions：
Check whether the barcode reader is installed properly, the barcode reader should be
perpendicular to the instrument bottom. The distance between laser release point and
reagent/sample area should be more than 60mm.The Laser bean should not be blocked
by anything.

（2）Unable to recognize sample barcode information

Possible reasons:
1） The barcode is too narrow
2） The barcode type is not supported by the barcode reader
Solutions:
Read the user manual and check the barcode type and printing width whether they can be
matched by the barcode reader.

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（3）Sample/reagent area door switch doesn’t work
Possible reasons：
1） Connection cable not well connected
2） Magnetic switch malfunction
3） The magnet inside the Sample/reagent area door unable to be close to the
magnetic switch
4） M600-02-E04 PCB board malfunction
Solutions：
1） Check the cable connection between M600-02-E04 and magnetic switch. Check
the flat cable connection to the main PCB board of the Maglumi analyzer.
2） Use a magnet material to get close to the magnetic switch, and the buzzer on
M600-02-E00 PCB board should have a “beep” sound. At the same time, it
should be short circuit between both cables of the magnetic switch. If not, then
the magnetic switch should be replaced.

Fig 3.7 Magnetic switch for Sample/reagent area

（4）Sample area cannot detect inserted sample rack
Possible reasons：
1）The head part of the sample rack is too short to be detected by the sensor
2）M600-02-E04 interface PCB board malfunction
3）Sample rack detecting sensor on M600-02-E04 malfunction
Solutions：
Use other sample rack or tool to block the sensor on M600-02-E04 and check
whether it can be detected. If not, so the sensor on M600-02-E04 is broken and need to
be replaced.

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 Fig 3.8 M600-02-E04 PCB board
（5）Abnormal speed of the reagent shaker
Possible reasons：
1）Affected by strong mechanical resistance
2）06-E02 PCB board malfunction
Solutions：
1）Check the cable connection of shaker motor and 06-E02 bonding wire
2）If you can move the shaker freely and feel no resistance, this indicates
M600-02-E00 malfunction and need to be replaced
3）If the shaker blocked when move to a certain position, this indicates this position
exist some mechanical resistance.
4）If there is a warning even the shaker moving normal, use the multi-meter to
measure the voltage at TP13 testing hole in M600-02-E00 PCB.

Fig 7.3 06-E02 shaker speed detecting PCB board

（2）Cooling function in reagent sample/reagent area not work properly

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Possible reasons：
1）Temperature sensor malfunction
2）Fan malfunction
3）M600-02-E04 PCB board malfunction
4）Peltier malfunction
Solutions：
1）Use multi-meter to measure the yellow signal wire at J4,to check the output signal
of the temperature sensor.
2）Check whether the fan can work normally. If it doesn’t move, then check the
voltage of J8 socket. If the voltage is +24 Volts, then you need to replace the fan.
3）Check the voltage between red and black wire of J3 socket. If the voltage is +24V,
it indicates that the peltier is failure and need to be replaced. If there is no voltage or the
voltage is very low, then replace M600-02-E04 interface board.

（2）Reagent area cannot detect inserted Reagent

Possible reasons：
1）The head part of the reagent kit (which is used for pressing the detecting switch) is
too short
2）M600-02-E04 interface PCB board malfunction
Solutions：
Use other reagent box or tool to press the switch on M600-02-E04 to check whether it
can be detected. If not, then this indicates that the switch malfunction and need to be
replaced.

3.3.6 Common troubleshooting for pipetting Pump

（1）Waste pump (for washing hole) not working

Possible reasons：
1）Waste pump malfunction
2）Blocking in waste tubing or waste pump
3）M600-02-E00 PCB board malfunction
Solutions：
1） Check whether both cables on the waste pump is connected well. And then check
whether the pump moving during the system sucking waste. If the pump is not
moving, then check the voltage of the red wire on the waste pump, the normal
range is 12-20V. If the voltage is out of range, replace the M600-02-E00 PCB
board. If the voltage is within range but the pump not work and in high
temperature, this means the pump malfunction and need to be replaced.
2） Check whether the tubing or the connector is blocked by crystal.

（2）Pipetting diluter pump cannot initialize

Possible reasons：

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 1）Diluter pump is blocked by system liquid crystal
2）optical sensor in diluter pump is corroded by system liquid
3）M600-02-E00 PCB board malfunction
Solutions：
1） Check whether there are some crystals block the motor or the connector of the
diluter pump. Power off the analyzer, use screw driver to rotate the screw at the
bottom of the motor and check whether the motor can move smoothly. If there is
some friction or the motor can’t rotate totally, this indicates the connecting point
between the motor shaft and the pump is blocked and the pump needs to be
replaced.

Fig 7.7 Use screw driver to rotate the screw at the bottom of the motor
2) Disassemble the diluter pump optical sensor and check whether it is corroded by
system liquid. At the same time check the D12 LED indicator light and measure
the voltage of TP12 test hole to check whether the sensor is damaged, if it is,
then change the pump.
3) If the pump works well and no blocking by any crystal material, replace the
M600-02-E00 PCB.
（3）Abnormal washing liquid injection of the pipetting needle
Possible reasons：
1）Washing control valve malfunction
2）Vacuum pump malfunction or not connected well
3）Pipetting probe or tubing blocking
3）M600-02-E00 PCB board malfunction
Solutions：
1）Check the cleaning valve voltage, the voltage should be >23V if it works well
2）Check the voltage of vacuum pump. If it work well, the voltage should be >23V
3）If above voltages are normal but the liquid in the tube cannot move, this indicates
the valve or vacuum pump malfunction; If the voltages abnormal, this indicates
cable connecting not well or M600-02-E00 PCB board malfunction.
4）Check whether there are some crystal or other material block the tubing system.

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4. Component 03-incubator &Wash station &Pusher

4.1 M600-03-E00-Washer PCB layout and function

4.1.1 M600-03-E00 PCB

Figure 4.1 M600-03-E00 PCB

4.1.2 M600-03-E00 PCB functional description

（1）Wash lift control and status

（2）Wash transport control and status
（3）Back transport control and status
（4）Wash pump control
（5）Peristaltic pump control
（6）Washer station overflow detection and warning
（7）Wash station entrance detection
（8）Incubator temperature control and status
（9）Incubator movement control and status
（10）Incubator cuvette number scanner
（11）Pusher control and status
（12）Wash load control and status
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（13）Sample load control and status

4.1.3 M600-03-E00 specification

TYPE Label Description remark

D1 Wash transport sensor LED and test hole green
TP2 When transport at the initial position, the sensor is not
blocked by anything, the LED will on, and TP2 voltage
<200mV; But when the sensor is blocked, LED will off,
and Tp2>4.5V
D2 Wash lift hall sensor LED and test hole green
TP3 When the lift at the initial position, the LED will on and
TP3 voltage <200mV;
Other positions, LED off, Tp3>4.5V
D3 Back transport sensor LED and test hole green
TP18 When the back transport at the Initial position or the
sensor is not blocked, the LED will on and TP18 voltage
<150mV; But if the optical Sensor is blocked, LED will
off, Tp18 voltage >4.5V.
D4 Pusher sensor LED and test hole green
TP5 When the Pusher block the sensor (pusher at initial
position), the LED will off and Tp8 voltage >4.5V; And if
the pusher located in other positions, the pusher cannot
LED block the sensor, so the LED will on , and TP8 voltage
test hole <200mV
D5 Wash load incubator position LED and test hole green
TP8 When wash load at incubator position, the incubator
position optical sensor will be blocked, the LED should
off and TP8 voltage >4.5V;
When incubator load at other positions, and it cannot
block the sensor, the LED will on, TP8 voltage <200mV
D6 Wash station entrance reflex sensor LED and test green
TP7 hole
When there is a cuvette in the wash station and block
the sensor, the LED will on and TP7 voltage >4V
Otherwise LED off, TP7<500mV
D7 Liquid detection LED and test hole green
TP6 When the two wash needles detect liquid, LED on,
Tp6<1V; No liquid, LED off, Tp6>4.5V
D8 Wash load at wash position LED and test hole green
TP9 When wash load at wash station position, the wash
station optical sensor is blocked, LED off, TP9>4.5V
When wash station at other positions, wash station

24
 optical sensor is not blocked , LED on, TP9 <200mV
D10 Incubator cuvette detection LED and test hole green
TP10 When there is cuvette under the sensor, the LED will on,
TP10 voltage <300mV; No cuvette under the sensor,
LED off, Tp10 voltage >4.5V
D12 Incubator initial sensor LED and test hole green
TP11 When incubator at initial position, LED on, Tp11<300mV
When incubator at other positions, LED off, TP11 >4.5V
D17 Pipetting load incubator position LED and test hole green
TP16 When load at incubator position, incubator sensor is
blocked, LED off, Tp16 voltage >4.5V
Other positions, the sensor will not blocked, LED on,
and tp16 voltage <200mV
D18 Pipetting load back transport position LED and test green
TP17 hole.
The wash station sensor can be blocked when pipetting
load at back transport position, LED off, tp17 >4.5V
Other positions, LED on, tp17 <200mV
D9 Program debugging indicator LED, developers use only blue
D11 Program debugging indicator LED, developers use only blue
D16 +3.3V power indicator and test hole yellow
TP14
D15 +5V power indicator and test hole yellow
TP13
D13 +24V power indicator and test hole yellow
TP12
Fuse F1 +24V fuse 5A
upgrade J4 upgrade interface
interface

4.2 Wash station function description

4.2.1 Wash station interface function

（1）03-E01
Right Pipetting area temperature control, wash station interface PCB and Circuit switching
device

25
 Fig4.2 03-E01 PCB
（2）03-E02
Hall sensor to detect the initial position of wash lift
（3）03-E03
Optical sensor to detect initial sensor of the wash transport
（4）03-E04 interface PCB
Back Transport initialization and positions detecting by optical sensor

4.2.2 Incubator Interface PCB function description

（1）M600-03-E05
Control for the incubator temperature, heating, motor, encoder, Hall sensor, temp
sensor and other parts. The Incubator movement and position record are controlled by the
encoder. Hall sensor can sense the magnet field, then output different voltage signal to
control the incubator to find the initial position.

Figure 4.3 M600-03-E05

26
4.2.3 Loader Interface PCB Function Description

（1）M600-03-E08
Interface for motors and sensors which controlled the washer loader and sample
loader. Each loader has two optical sensors located in the left and right, using for control
the movement of the loader.

Fig 4.4 M600-03-0E08 PCB

4.2.4 Pusher Interface PCB function description

（1）02-E02
Pusher includes Motor, encoder, optical sensor and other parts.
Encoder can control the Pusher movement and record the position. And the initial
position is detected by the optical sensor.

27
 Figure4.5 Pusher

Figure4.6 02-E02 Pusher interface PCB

4.3 Failure diagnosis

4.3.1 Washer station common problems and diagnosis

（1）Washer transport unable to move or initialize

Possible reasons:
1） The washer transporter metallic plate cannot block the right side optical sensor
when initialize.
2） Washer transport optical sensor broken
3） Installation location of the optical sensor or washer transport gear’s metallic block
28
 is not well.
Solutions:
1） Check the connection of the cables.
2） When washer transport doesn’t block the sensor, green light D1 located on
M600-03-E00 PCB should be on. And the D1 will off when the sensor is blocked.
3) Before initialize, if the metallic block is in the right side of the sensor, then the
washer transporter can’t go back to the left initial position when initialize.
4）Metallic block should not block the sensor when move to the left side, if block the
sensor, adjust the optical sensor position.

Figure4.7 Washer transport Initial position

（2）Wash lift can’t initialize

Possible reasons:
1） Initial Hall sensor broken
2） Initial position too high, magnet is higher than the sensor.
Solutions:
1） Manually move the wash lift, when the magnet near to the hall sensor, D2 which
located on M600-03-E00 PCB should be on, and when the wash lift away from
the sensor it should be off.
2) If D2 never on, use a magnet to touch the hall sensor, if D2 is still off, replace the
PCB M600-03-E00 PCB or 03-E02; If D2 is on, means the hall sensor position
changed, need to do the alignment.
3）If there is no status change for D9 when washer lift located at any position, means
the hall sensor broken.

Figure 4.8 Wash station lift hall sensor

（3）Washer Lift move upward/downwards not possible
Possible reasons：
29
 1）Wire loose for the encoder
2）Mechanical resistance
Solutions：
1）Check the encoder wire/socket connection and fix it if it’s loosen
2）Check if there is corrosion on washer lift axis and clean it if necessary
3）Switch off analyzer, move the washer lift manually to check if there is mechanical
resistance at specified position
4) If there is no problem after check as above, please replace the M600-03-E00 PCB

（4）Back Transport move abnormally, not initialize or move left/right not possible
Possible reasons：
1） Optical sensor mistake or broken
2） Blockage in the drill hole of the Back Transport fork
3） Optical sensor displacement due to large gap between geared fork and motor
wheel
4） Not proper matched between incubator, pusher and back transport
5） Motor is loose
Solutions：
1） Disassemble the fork to clean the blockage/dust in drill hole
2） Check the cooperate between geared fork and motor wheel
3） Push the fork manually, observe the indicator LED D3 on the M600-03-E00 PCB,
normally the LED should have status changes during the movement of the fork,
and only the sensor match the drill hole it will on.
4） If D3 doesn’t change status from on to off all along the movement, please check
the output voltage of testing hole TP18 on 03-E00(Normally the value is over 4V
when drill hole of fork faces against optical sensor, otherwise is less than 0.5V ).
If the voltage is out off the normal value, it needs to change the optical sensor. If
the voltage is ok, it means there is problem from M600-03-E00 PCB which
should be replaced.
5） Check each position fitted between the right Pipetting channel and the incubator
slot (16 slots) with a piece of cuvette. Adjust the position properly in the service
software if necessary. Otherwise, replace the fork if it’s bended.
6） Check and adjust the driving motor position to match with the fork gear

（5）Wash Pump dispensing abnormal

Possible Reasons：
1） Mechanical friction resistance
2） 03-E05 interface PCB for Wash Pumps or M600-03-E00 PCB failure
Solutions：
1） Check the wire connection of wash pump and prevent any obstructions against
the rotate wheel of the pumps.
2） Exchange the wire connection of the abnormal pump with the one in good
condition, if it works, that means there is filature for 03-E00 which should be
replaced, If it doesn’t work, then replace the wash pump.

30
 (6) Peristaltic Pump aspiration abnormal, overflow detection failure
Possible Reasons：
1）Too much contact pressure of the peristaltic pump wheels
2）one or more washer needles are clogged
3）Overflow sensors(a pair of hooks) broken or incorrect connection
4）liquid drops remains between injection needle and aspirate needle
5）M600-03-E00 PCB failure
Solutions：
1） If peristaltic pump could not rotate during aspiration, check the wire connection
between the pump and back penal PCB. Otherwise, replace M600-03-E00 PCB.
2） Check the tension of the aspirate tubing by adjusts the screw.
3）Clean the washer needle.
4）Check the connection of the flooding sensor and the soldering junction on 03-E01
Interface PCB, normally there are two groups of connectors, one group connect to the
injection needles and the other connect to three aspiration needles( Figure 4.9 ).
Check the sensor connection by multi-meter.

Figure 4.9 Flooding sensor

5) Observe the LED D7 status on PCB M600-03-E00.
Normally it becomes on while aspiration needles touch the liquid inside the
cuvette. If D7 is always the same status no matter the aspiration needle inside the
liquid or outside, replaces the M600-03-E00 PCB.

(7) Washer Reflect sensor can’t detect cuvette when initialize with clear cuvette.
Possible Reasons:
1) The sensitivity of the sensor is not adjusted well.
2) Broken of the sensor
3) PCB M600-03-E00 failure
Solutions:
1) Measure the voltage of TP7 located on PCB M600-03-E00, normally the voltage
should be > 4V when there is cuvette there, otherwise the voltage should be <
500mv. If the voltage is abnormal please check the connection of the sensor,
whether it’s good or not, or replace the sensor.

31
 Figure 4.10 installation of the reflect sensor.
2) Measure the voltage of TP10 located on PCB M600-03-E00. Enter service
software and use the washer loader to push a cuvette to the wash station, then
adjust the W2 potentiometer, to make the D10 on (clockwise make it off, and
anticlockwise make it on). After adjustment the indicator light D10 should be on
when have cuvette block the sensor and off without cuvette.

Figure 4.11 Potentiometer of the washer reflect sensor

3）If the sensor is good, and also there is no use after adjust the sensitivity, so the
problem is from the PCB M600-03-E00, replace the PCB.

4.3.2 Possible problems and solution for Incubator

（1）Incubator could not find initial position

Possible Reasons：
1） Hall sensor deformation
2） Hall sensor broken
Solutions：
1） Push the incubator towards to the home position, when the magnet get very
closed to the hall sensor (at the bottom end of the incubator interface PCB),
indicator LED D12 on the M600-03-E00 will be on.
2） If LED D12 is not on anyway as above, use a piece of magnet and place it very
32
 close to the hall sensor. If LED D12 is on, please correct the hall sensor
deformation manually. However, if D12 is still off, there may be problem from PCB
M600-03-E00 or PCB M600-03-E05, which should be replaced.
3） If LED D12 always be on/off no matter where the incubator located, hall sensor
may be broken. Change the hall sensor.

Figure 4.12 Hall sensor

（2）Incubator not move normally , not possible to move forward/backwards
Possible Reasons:
1）wire disconnection to the encoder
2）Too much resistance during movement
Solutions:
1）Check the wire connection to encoder. Tighten the socket if it’s loosened.
2）Check two axis of incubator if there is corrosion. Clean it if necessary.
3）Observe the analyzer to see if there is warning message when incubator move to
some specified position. Switch off the analyzer, move the incubator manually to
feel if there is too much frictional resistance during the movement.
4）If there is no problem after check all above, replace M600-03-E00 PCB.
（3）Incubator temperature is out of normal range
Possible Reasons:
1） Temperature sensor broken
2） Heating resistance property changed due to aging
Solutions:
1） Recheck the temperature with high accurate thermometer
2） If temperature is within normal range but there is warning message in User
software, please correct the temperature setting from Service <Error card> after
the analyzer warn up for more than 1 hour.
3） If temperature is out off normal range (36.8 ℃ + 0.5), please adjust the
potentiometer W1 on PCB M600-03-E05 to correct temperature (Set it in
anticlockwise, normally 1 cycle to enlarge 0.1℃ for the incubator).

33
 Figure 4.13 W1Potentiometer on PCB M600-03-E05
4) If the temperature is far away from normal range, this could not be correct by
adjusting the potentiometer, it needs to check the temperature sensor or replace
the PCB M600-03-E05.

4.3.3 Possible problems and solution for Pusher

（1）Pusher could not be initialized

Possible Reasons：
1） Optical sensor broken or wire disconnection
2） Outside interference
Solutions：
1. Check the output voltage for the optical sensor. To measure the voltage of the
solder junction (yellow wire, see bellowing Figure 4.14).
Meanwhile observe the indicator LED D4 located at M600-03-E00 PCB, check
whether it have signal change (on and off) during the push movement. Otherwise
replace the PCB (M600-03-E00) to fix the problem.

Figure 4.14 Optical sensor on 02-E03 interface PCB

2. Check the motor/encoder wire connection and fix it if it’s loosened. Keep the
cable of 02-E03 interface PCB away from strong power source to avoid
34
 electronic interference.
（2）Pusher could not complete movement within specified time, not possible to
move forward/backwards, not reach the correct position
Possible Reasons:
1） Mechanical resistance outside
2） Wire/socket disconnection or loosen to encoder
3） Wrong positioning due to rotating bell aging
Solutions:
1) Check the main cover, washer channel, chamber transport if there is mechanical
force/resistance against the pusher. If necessary, adjust the parts accordingly.
2) Check the wire/socket connection of encoder and fix it if loosen
3) Disassemble the pusher and adjust the metal belt tension. Then check the
position from service software.

4.3.4 Possible problem and solution for Loader

(1) Washer loader move abnormal, not initialize or move forward\backward failure
Possible reasons:
1) Cuvette crash between the washer and incubator
2) The sensor is not adjusted well or broken
3) PCB M600-03-E00 broken
Solutions:
1) Check whether there is cuvette in incubator or washer cause the cuvette crash.
2) Check the incubator positions, whether all the slots match the wash station well
3) Check the optical sensor of the loader; observe the indicator light D5 and D8 locate
on PCB M600-03-E00, whether the light has signal change according to the sensor
status, otherwise the sensor is broken.
4) When the loader move to one side (left or right), make sure the plate which on the
belt should block the senor. Otherwise it may have a dead zone and cause the
initialize fail. Need to adjust the sensor position.

35
 Figure 4.15 the sensor of the loader

Figure 4.16 the loader is on the side (left & right)

5）If the loader is not locked after the machine is on, but the sensor is good, so the
problem may from PCB M600-03-E00.

36
5、Component 04-chamber

5.1 layout of M600-04-E00-Chamber PCB and function

5.1.1 M600-04-E00 PCB

Fig5.1 M600-04-E00

5.1.2 M600-04-E00 function describing

（1）Control of chamber transport

（2）Control of chamber lift
（3）Control of starter pumps
（4）Control of waste pumps
（5）Control of PMT’s high voltage
（6）Control of blue light’s voltage

5.1.3 M600-04-E00 electronic components

Type Label Explanatory Notes Remarks

LED D6 Program debugging LED blue
D12 Chamber liquid level detection LED and testing green
TP12 hole
LED is on when no liquid remaining between detect
37
 needle and waste liquid aspirating needle, and the
voltage of TP12 is less than 200 mV;
LED is off when the needles detect liquid level, and
the voltage of TP12 is more than 4.5V.
D13 Starter 1 pump optical sensor LED and testing hole.
TP13 The LED is on when starter 1 pump optical sensor is
blocked, and the voltage of TP13 is less than
200mV;
The LED is off when starter 1 pump optical sensor is
unblocked, and the voltage of TP13 is more than
4.5V;
D14 Starter 2 pump optical sensor LED and testing hole.
TP14 The LED is on when starter 2 pump optical sensor is
blocked, and the voltage of TP14 is less than
200mV;
The LED is off when starter 2 pump optical sensor is
unblocked, and the voltage of TP14 is more than
4.5V;
D16 Indicator LED of chamber lift and testing hole.
TP11 The LED is off when chamber lift is located at the
initial position where the optical sensor is unblocked,
and the voltage of TP11 is more than 4.5V;
The LED is on when chamber lift is located at other
positions where the optical sensor is blocked, and
the voltage of TP11 is less than 200mV;
D17 Chamber transport LED and testing hole.
TP9 The LED is off when chamber transport is located at
the initial position where the optical sensor is
blocked, and the voltage of TP9 is less than 200mV;
The LED is on when chamber lift is located at other
position where the optical sensor is unblocked, and
the voltage of TP9 is more than 4.5V;
D1 +3.3V power LED yellow
D2 +5V power LED yellow
D3 +15V power LED yellow
D4 +24V power LED yellow
D19 -15V power LED yellow
fuse F1 24V power fuse 5A
Upgrade J7 Program upgrade interface
interface

38
5.2 Chamber function and trouble shooting

5.2.1 Chamber interfaces function description

（1）04-E01 interface PCB behind the chamber

Connect to the other chamber interface PCBs.
Connect the interface PCB of the photomultiplier (PMT)

Fig 5.2 04-E01PCB

Key signal testing hole:
M1: PMT-driving analog voltage, normal range 1.5V～2.2V
M2: Output signal of the chamber entry optical sensor.
M3: Signal of liquid level detection. The voltage of M3 is more than 4.5V when no liquid
is detected by the detective needle and waste liquid aspirating needle, D2 is off as
well; D2 is on and the voltage of M3 is less than 2.4V when detective needle and
waste liquid aspirating needle drop into liquid level.
M4: Signal of the optical sensor which is used to check the initial position of the
Chamber lift. The voltage of M4 is more than 4V when the optical sensor is blocked;
otherwise the voltage is less than 0.5V.
M5: Signal of the Chamber transport initialize optical sensor. The voltage of M5 is more
than 4V when the optical sensor is blocked; otherwise the voltage is less than 0.5V.
（2）PCBs for chamber transport and chamber lift
These two PCBs are used to check the initial positions
（3）04-E07 PCB of blue light
This board is used to supply a stable voltage for the blue light.
（4）PCBs of PMT
04-E04 PCB for high voltage, 04-E05 PCB for Signal amplification and 04-E06 PCB is
bleeder circuit.
（5）M600-04-E09 PCB of starter pump

39
 Connect with pump motor of starter1and 2, optical sensor
Connect with chamber waste pump

5.2.2 Possible problems and solution for Chamber

（1）"Chamber transport not initialized!"

Possible reasons:
1）The impact of external resistance
2）Damage of optical sensor for the chamber transport
3）Damage of 04-E01 interface PCB
4）Damage of M600-04-E00 PCB
Solutions:
1）Turn the cross-gear manually to check whether there is some resistance ( maybe
crystal or outside force).
2）Manually move the cross-gear and check whether the LED D4 on the 04-E01
interface PCB will on and off
3）If D4 signal indicate normally, then need to replace the M600-04-E00 PCB.
4）If D4 signal is abnormal, test the voltage of the M5 testing hole on the 04-E01 PCB,
change the interface PCB of the optical sensor when the voltage is abnormal, and
change the 04-E01 PCB when the voltage is normal.

Figure 5.3 Interface PCB of the optical sensor for chamber transport

（2）"Chamber transport move not possible!"

Possible reasons:
1） The cross-gears of the chamber transport are blocked by the external resistance
2） The positions of the 4 cross-gears are not good.
Solutions:
1） Check the positions where the cuvette is easy to be blocked, for example: entry
and exit of the chamber. Observe whether there is outside force.
2） Check the relative position of the four cross-gears, the angle of the previous three
cross-gears are the same, but the No.4 is 45° different with the others.

（3）"Chamber lift not initialized!"

40
 Possible reasons:
1） Damage of the optical sensor for the chamber lift.
2） The position of the chamber lift is too high before initialization.
3） Damage of the M600- 04-E00 PCB or 04-E01 interface PCB.
Solutions:
1） Move the chamber lift manually to block or unblock the optical sensor, check
whether the status of D3 on the 04-E01 interface PCB will be changed. (If the
sensor works normally, when being blocked, the LED is on, otherwise the LED is
off.)
2） If the status of the D3 doesn’t change, test the voltage of the test hole on the
04-E01 interface PCB. Replace the optical sensor interface PCB when the
voltage is abnormal, while change the 04-E01 PCB when the voltage is normal.
3） If the status of the D3 changed normally, the M600-04-E00 PCB needs to be
changed.
4） The entry and exit of the chamber should not be higher than the chamber channel.
Otherwise it’s unable to initialize.

Figure 5.4Interface PCB of the optical sensor for chamber lift

（4）"Chamber lift move up/down not possible!"
Possible reasons:
1）Cable of the encoder was loose
2）Moving-resistance of the chamber lift is too large
3）The waste needle of the chamber is blocked by cuvette
Solutions:
1）Check the encoder cable connection, or bad contact of the terminal.
2）Check whether the chamber lift is rusty, clean it if necessary.
3）Turn off the analyzer, move the chamber lift manually to check whether there is
resistance; Observe whether there are crystals besides the exit and entry of the
door; if the chamber lift always alarm at the same position, check the mechanism
resistance.
4）Disconnect the cable of the PMT, open the cover of the chamber, do the “Measure
Cuv” from service to observe whether each vial of the cuvette is just below the
injector. Or whether the vial will be blocked by the suction needle, if blocked,
adjust the parameter of the chamber transport or position of the injector.
5）If all above are normal, change the M600-04-E00 PCB.

41
5.2.3 Chamber pumps trouble shooting

（1）Starter 1/2 inject abnormal

Possible reasons:
1）Check the cable connection.
2）Damage of the starter pump’ s optical sensor.
3）Damage of the 04-E00 PCB.
Solutions:
1）Check cables connected the optical sensor or motor of the starter pump, and
whether the contact of the terminal is OK.
2）If the cables connected of Starter Pump is OK. Manually rotate the Starter Pump,
observe if the status of Starter Pump’s detecting light will change. If not, it means
the Starter Pump maybe damaged and should be changed.

Figure 5.5 white cable connector of Starter Pump on M600-04-E09

3）If there is only one pump have problem, replace the optical sensor and motor
cables with each other, and run the start pumps again. If the pump which have
problem can work normally, the M600-04-E00 need to be changed; if it still have
problem, then the pump needs to be changed.
（2）" Chamber soak not soak normally!"
Possible reasons:
1）Waste pump doesn’t work.
2）The suction needle or the soak pump is blocked.
3）The wire between the suction needle and detection needle is loose or short circuit.
4）Water drop between the suction needle and detection needle.
5）There is no height difference between the suction and detection needle.
6）Damage of 04-E01 or M600-04-E00 PCB
Solutions:
1） Check whether the two cables of the waste soak pump are loose.

42
 2） Observe whether the pump works when the analyzer do the soak process. If the
pump doesn’t work, test the voltage of the red power cable, the normal range
should be 14¬ 20V, if the voltage is out of range, change the M600-04-E00 PCB.If
the pump works, but the waste cannot be taken out, check whether the suction
needle or the soak pump is blocked by some solid particles.

Figure 5.6 Soak pump of chamber

3）If the waste can be taken out by the soak pump, but the software still alarm, please
check the height difference between the suction needle and detection needle.
Actually, the waste needle should be longer than the detection needle. If it is not
like this, adjust the height of the needle.

Figure 5.7 suction needle and detection needle of the chamber

4）Check the connection. The signal wire is connected to the detection needle, and the
suction needle is grounded.

Figure 5.8 wire for liquid level detection

5）Check whether the LED D2 on the 04-E01 interface PCB in the back of the
chamber will be off when the suction needle and detection needle are inside the
liquid. If not, change the 04-E01 PCB.
6）If there are water drops between the suction needle and detection needle, clean it.

43
5.2.4 PMT trouble shooting

Figure 5.9
（1）Measuring result is 0
Possible reasons:
1) Bad contact of the power wire or signal wire of the PMT.
2）Damage of the PMT PCB.
3）Damage of the M600-04-E00PCB.
Solutions:
1）Check the connection of the signal wire and power wire of the PMT.
2） Disconnect the power wire of the PMT, run the “background” in Service software.
At the same time, test the voltage of the test hole on 04-E01 PCB. The normal
range should be 1.5V ～ 2.2V. If the voltage is out of range, change the
M600-04-E00 PCB.
3）If all the above checking are Ok, change the whole PMT component.
（2）the value of the background is high and unstable
Possible reasons:
1）light leakage of the PMT or chamber.
2）PMT or the ground wire is not grounded well.
3）Damage of the PMT component.
Solutions:
1）Re-install the PMT component and lock it tightly; Use some black cloth cover the
chamber and then test the background again. If the value of background is
decrease, maybe there is light leakage from the PMT or the chamber. If the value
still be high and unstable, change the whole PMT component.
2）Check the analyzer grounding. Check the connection between the PCB of PMT
and the analyzer’s metal shell. There should not be insulative materials contact to
the metal.
3）If there is no light leakage and the grounding is good, but the value of the
background is still high and unstable. Change the PMT component.
（3）Blue LED defect: “No cuvette in chamber”, or the value of “Ref led
on” in the background is >30W or <1W
Possible reasons:
1）Damage or aging of the blue LED.
2）Damage of the 04-E07 PCB

44
3）Damage of the M600-04-E00 PCB
4）Parameters of the“D/A Ref led” and “Min.value.cuv” not accurate
Solutions:
1） Enter service software; test the value of “Ref led on” from the background.
Normally, the value should be >14W and <23W, if the value has a little difference,
adjust the value of the “D/A Ref led” to make it in the normal range.
2） If the value of the “Ref led on” in the background is near the value of
“background/closed”, and while increasing the “D/A Ref led” have no effect to
increase the value of “Ref led on”. That means the blue LED is not on. Check
whether the red LED on the 04-E07 PCB is on, if the red LED is on, change the
04-E07 PCB, if it’s off, then change the M600-04-E00 PCB.

Figure 5.10 04-E07 PCB and the red LED

3） Adjust the parameter of the “Min.value.cuv”
Min.value.cuv = (A+B)/2
A: Value of background ‘Ref led on” when cuvette block the PMT.
B: Value of background ‘Ref led on” when the PMT is unblocked.

45