Professional Documents
Culture Documents
Renewable Hydrogen Production From Biomass and Wastes 2021 Bioresource Techn
Renewable Hydrogen Production From Biomass and Wastes 2021 Bioresource Techn
Bioresource Technology
journal homepage: www.elsevier.com/locate/biortech
Preface
A R T I C L E I N F O A B S T R A C T
Keywords Growing consumption of fossil reserves to meet the rising demand of energy has led to climate deterioration and
Renewable biohydrogen simultaneous waste generation, urging modern society to find sustainable energy resource that can meet the
Dark fermentation growing energy demands and reduce greenhouse gas emissions and carbon footprints. In this aspect, hydrogen
Microbial electrolysis cell
(H2) is one of the most promising sustainable clean fuels that has gained significant interest in recent years. This
Bioresources
article highlights the major research progress on biohydrogen production from renewable bioresources such as
Industrial waste
Gasification organic wastes, lignocellulosic biomass, algal biomass, and industrial wastewaters. It summarizes the research
highlights of manuscripts published in the special issue (VSI: ReBioH2-2020), which contains twenty-two arti
cles, including seven critical reviews and fifteen research articles, focusing on biotechnological and thermo
chemical routes for biohydrogen production from renewable feedstocks. The major findings of the research
works in this special issue can be used as a road–map for sustainable renewable hydrogen production from
bioresources.
https://doi.org/10.1016/j.biortech.2021.125024
international economic development. challenge nowadays with a growing economy. In recent years, dark
Towards these ends, this special issue published new logical research fermentation of organic-rich waste streams demonstrated an economical
articles to accomplish a renewable hydrogen energy society in the near and environmentally friendly way for bioremediation of organic wastes,
future. The objective of this special issue is to extend knowledge and simultaneously producing biohydrogen. However, the complex struc
recent progress in renewable biohydrogen production. Biohydrogen ture of organic waste and low hydrogen yield are major obstacles for
production from waste organic materials could meet the desired criteria large-scale activities. Recent reports showed that the coupling of
for a decarbonized economy. However, biohydrogen is still facing fermentation with biological anaerobic pretreatment could effectively
challenges of process optimization and scale-up. Additionally, this issue improve hydrogen production. For example, Paillet and co-workers
emphasized the various biotechnological, and thermochemical routes investigated the effect of three heat shock pretreatment conditions on
such as dark fermentation, biohythane production, utilization of recycled organic-rich efficacy, including initial heat shock (90 ◦ C, 30
microalgae, lignocellulosic biomass and organic-rich waste streams for min), systematic heat treatment and without any heat treatment.
biohydrogen production. Moreover, this review also emphasize inte Optimal conditions achieved with systematic heat treatment, yielded a
grated biorefinery development and technoeconomic, socioeconomic maximum of 17.2 ± 3.8 mLH2/gVS with a 331% increase in stable
studies for biohydrogen production for commercial viability. productivity of 1.51 ± 0.29 mLH2/gVS/h after the 8th recycling cycle
(Paillet et al., 2021).
2. Recent advances in biohydrogen production: Approaches and Renaudie et al. (2021) investigated dark fermentation activities and
challenges developed a hollow fiber continuous liquid/ gas (L/G) fermenting
membrane bioreactor for continuous long-term hydrogen production
2.1. Dark fermentative hydrogen production without seeding. They evaluated the membrane’s role in retaining
hydrogen-producing bacteria using waste industrial sludge, which
Hydrogen and gaseous fuels from renewable feedstocks have opened reduced in-between seeding. A steady-state reactor operating for 551
up future possibilities to substitute fossil energy resources to combat days without seeding produced 1.1 ± 0.2 mol H2/mol sugar (added
global warming and energy needs. Renewable biohydrogen from an initially), and maximum productivity of 135 ± 22 mL H2/L/h was
integrated biorefinery is critical for developing a low carbon fuel achieved using acetate and butyrate metabolites. They noted that an L/G
economy with biowaste remediation in an environmentally–friendly membrane served as an interface for in situ hydrogen extraction and
way. In this special issue, the authors review the potential challenges; preserved the Clostridium beijerinckii, Clostridium pasteurianum, and
environmental, economic, and social aspects that need to be tackled for Enterobacter sp. In another article in VSI ReBioH2-2020, Sarkar and his
commercial-scale production (Dahiya et al., 2021). colleague showed the optimized and scale-up activity in uncontrolled
Dark fermentative biohydrogen production involves the anaerobic pH conditions for H2 and volatile fatty acids (VFAs) production using
digestion of various renewable resources such as crop residues, agri brewery spent grains (BSG) (Sarkar et al., 2021). Additionally, they
cultural biomasses, organic waste, the organic fraction of municipal evaluated the impact of varying pH (4–10) on acidogenic fermentation
solid waste, and organic-rich wastewater into carbon dioxide-free of BSG at different scales (2, 5 and 10 L). The two-stage study showed
hydrogen. It is a highly efficient process with high hydrogen produc that the first stage stabilized the pH, whereas the second stage optimized
tivity and could develop long term energy projects using renewable hydrogen production. Moreover, the initial alkaline condition could
energy resources. Theoretically, 4 mol H2/mol hexose can be produced serve as a pretreatment agent to hydrolyze soluble carbohydrates, which
through the acetate (1), or butyrate (2) pathway. Comparatively, the could further increase the enzymatic yield and simultaneous bio
butyrate pathway produced a low hydrogen yield (2 mol H2/mol hex hydrogen production by 15–90% with a maximum volume of 834 mL
ose). The investigation by Dauptain and co-workers revealed that and 10–50% higher volatile fatty acid (8936 mg/L). Extension of
hydrogen production could be significantly affected by inoculum, stor fermentation time could significantly favor short-chain fatty acids
age condition, and the microbial community. They showed that long- (SCFs) to medium-chain fatty acids (MCFs). This approach could be
term freeze-drying could improve hydrogen production in dark adopted and coupled to pretreatment with the simultaneous conversion
fermentation (Dauptain et al., 2021). of native biomass to biohydrogen and VFAs.
Rambau and co-workers highlighted the enrichment in biohydrogen
C6 H12 O6 + 2H2 → 2CH3 COOH + 2CO2 + 4H2 acetate pathway (1)
production using nano-metal oxides-based dark fermentation reactions
(Rambabu et al., 2021). They reported that the rice mill wastewater
C6 H12 O6 → 2CH3 CH2 CH2 COOH + 2CO2 + 2H2 butyrate pathway (2)
stream could be enriched in organics, which can be employed for
hydrogen production. Inclusion of NiO and CoO nanoparticles in the
2.1.1. State-of-art technologies for continuous hydrogen production
fermentation broth of wastewater received from Rice mill industries
aIn the past, several reports have been presented to the scientific
using Clostridium beijerinckii DSM 791 could further improved bio
domain for dark fermentative hydrogen production focusing on carbon
hydrogen production by 2.09- and 1.9-fold using 1.5 mg/L of NiO (~26
feedstock, reactor design, operational condition, and the nature of the
nm) and CoO (~50 nm) nanoparticles through acetate and butyrate
inoculum at a laboratory scale. However, further assessment of various
pathways. The results showed that fermentation could effectively reduce
controls for high production rate is needed to commercialize dark
77.6 and 69.5% of chemical oxygen demand (COD) using NiO and CoO
fermentation technology. Therefore, Park et al. (2021) reviewed the
metal oxides as quantified by the Gompertz kinetic model. François et al.
various operational methods, reactor configurations, and microbial
(2021) showed that endogenous dark fermentation of wine byproducts
communities for high rate hydrogen production. They concluded that
(grape must residues), could produce 35 L H2/L biomass and yield of 1.6
assembly improvement, bioreactor regulations, operational strategies,
mol/mol hexose respectively. They showed that endogenous microflora
and reactor configurations could impact the hydrogen fermentation
enriched in Clostridium spp. (butyricum, beijerinckii, diolis, and roseum)
technologies. They reported that a continuous high hydrogen produc
phylotypes could efficiently degrade sugars to hydrogen molecules
tion rate could be achieved by maintaining symbiosis between
without any addition of nutrient medium or heat treatment.
hydrogen-producing bacteria and biofilm-producing bacteria to avoid
washout. Use of mixed culture, maintaining production parameters such
2.2. Microbial electrolysis cell (Bioelectrical systems)
as hydraulic retention time (HRT), organic loading rate (OLR), pH,
substrate concentration, operating temperature, and microbial com
Bioelectrical systems (BES) are novel, highly promising bio
munity coupled to statistical analysis could lead to higher productivity.
electrochemical approaches nowadays for transforming organic mate
Disposal of the organic municipal solid waste fraction is a major
rials into valuable chemicals, fuels, electricity, or biohydrogen for
2
Preface Bioresource Technology 331 (2021) 125024
circular economy and clean energy production. Typically, microbial for development of sustainable technology that employs co-digestion of
electrolysis cells (MEC) are considered a promising BES system for animal waste and lignocellulosic biomass for biohydrogen production in
renewable biohydrogen production from organic resources that utilize a cost-effective way with higher efficacy compared to the mono diges
anodic bio-catalytic oxidation and cathodic reduction processes (Yang tion process.
et al., 2021). Self-sustainable MEC bioelectrochemical tools can trans
form organic carbon and waste organic water into hydrogen with low 2.4. Microalgal biomass for biohydrogen generation
energy requirements, i.e., >0.14 V compared to water electrolysis, i.e.,
>1.2 V. They showed that using a high catalytic, cost-effective cathode The photosynthetic microorganisms, cyanobacteria microalgae can
catalyst may promote commercial applicability of MEC process toward produce biological renewable hydrogen through microbial photolysis
self-sustainable high yield hydrogen production. Industrial wastewater and fermentation. In the photosynthetic process, microorganisms utilize
treatment strategies, energy recovery from waste and value-added sunlight to split H2O to produce in-situ hydrogen in the surrounding
products can be coupled by metabolic diversity. They showed that atmosphere. Microalgae are considered third-generation biomass, which
coupling of microbial electrolysis cell systems with other carbon-free have immense potential for biohydrogen and value-added chemical
biotechnologies such as pretreatment, pyrolysis, and fermentation production. Microalgal biohydrogen has gained global interest owing to
could collectively improve the enhanced rate of biohydrogen to 4.3 L its carbon–neutral source for sustainable energy. Salakkam et al. (2021)
H2/L-day and 50.4% yield. Moreover, chemical pretreatment of organic provided a detailed review focused on valorization of microalgal
wastewater stream can significantly convert organic wastes into simpler biomass for biohydrogen production and elaborated the various aspects
molecules that can be digested by exoelectrogens in MECs systems. The such as algal strains, cultivational practices, and harvesting. They have
study revealed that an enhanced rate of biohydrogen (0.59 m3 H2/m3/d) also provided the factors and characteristics that affect the overall
was reached using lignocellulosic biomass employing direct fermenta fermentation performance such as biomass pretreatment methods, dark
tion before MEC, wherein VFA produced in fermentation served as fermentation, photo fermentation efficacy, co-digestion with organic
feedstock to subsequent MEC utilization. To achieve high hydrogen materials, technoeconomic study, and application of advanced molec
yield, two-chamber microbial electrolysis cells separated with a biode ular biology for enhanced biohydrogen production.
gradable membrane showed significant improvement in hydrogen pro In this special issue, El-Dalatony et al. (2020) review the different
duction. Nafion (DuPont) has a widearray of membranes for hydrogen- metabolic pathways for microalgal biohydrogen production from the
producing microbial fuel cells, however, the high cost of membranes last five years while focusing on operational cost and economic viability
limits commercial applicability. Therefore, González and co-workers of microalgal hydrogen. In this in-depth review, they highlighted the
demonstrated a new cost-effective, eco-friendly membrane comprised major factors such as light, cell density, nutrition composition, and
of polyvinyl alcohol/ chitosan (PVA/ CS) for hydrogen production. A microalgae availability. Moreover, they summarized the different pre
long term operational study revealed that their PVA/CS membrane treatment approaches for high conversion efficiency. With that, they
could retain its stability for a month, yielding 974 ± 116 mL H2/g ac emphasize a new approach, i.e., microbial electrolysis cells for micro
etate at a production rate of 1277 ± 46 mL H2/Lcathode/d (González- algae biohydrogen production. In this review, they summarized various
Pabón et al., 2021). Biocompatible PVA/CS showed great potential for tools such as genetic, metabolic engineering and synthetic biology for
future hydrogen production with social, environmental and economic further improvement in microbial biohydrogen production via inhibi
importance. tion of competing pathways and oxygen tolerant hydrogenase.
In another study published in VSI, Li et al. (2021) demonstrated a Goswami and co-workers critically reviewed the different pathways
novel approach for biohydrogen production using Rhodobacter sphaer and enzyme involvement for economical production of hydrogen in
oides using a microbial electrosynthesis cell. When they used CO2 as a microalgae and discussed integrated bioprocess engineering strategies
carbon source and applied − 0.9 V vs. Ag/AgCl potential to the cathode, for improved hydrogen production. In their published article in this VSI,
they found R. sphaeroides produced 328 mL H2/L/day on a carbon Goswami et al. (2021) showed that the hydrogen production tendency of
electrode in an MES cell under optimized condition (Li et al., 2021). In microalgae is highly dependent upon strain selection, environmental
another report published in VSI ReBioH2-2020, the PROMETHEE II parameters, enzymes types, metabolic pathways, etc. Microalgae are
method has been displaced with two cation exchange membranes single-cell, photosynthetic aquatic microorganisms that mainly consist
(PSEBS SU22 and CF22 R14) and one bipolar membrane for high of carbohydrates, lipids, and protein. They can produce hydrogen via
hydrogen performance in MEC cells (Cardeña et al., 2021). They docu oxygenic pathways following direct photolysis and/ or indirect photol
mented membrane materials for this technology and filed a patent, ysis mechanistic pathways. They concluded that microalgae can be
where they developed a novel PSEBS SU22 for superior hydrogen efficient in wastewater treatment with cost-effective hydrogen produc
production. tion. Implementation of advancement strategies such as bioprocess en
gineering, selection of suitable pretreatment methods, use of
2.3. Biohythane production photosystem inhibitors, O2, scavengers, supplementations, co-culturing,
and genetic modification of strains could improve the direct production
Biohythane is notable and promising gaseous fuel consisting of bio of hydrogen (~56%) with reduction of overall production cost.
hydrogen (10%) and biomethane (30%) produced via two-stage anaer
obic fermentation of organic materials such as agricultural residues and 2.5. Bioconversion of agro-industrial residues to hydrogen
animal wastes through co-digestion with reduced nutrient demand.
Animal waste such as pig manure is categorized by the presence of high Lignocellulosic biomass is the most abundant, available and cheap
moisture with a significantly low carbon-to- nitrogen (C/N) ratio bioresource on Earth. For the most part, lignocellulosic biomass is
compared to lignocellulosic biomass such as rice straw. Thus, a study available in the form of agricultural and forest residues, aquatic plants,
published by Chen et al. (2021) showed the co-digestion of lignocellu and crops wastes, which have huge potential for commodity chemicals
losic biomass (e.g., rice straw) and animal waste (e.g., pig manure) to and fuels generation through thermochemical or biotechnological ap
attain nutrient balance using mesophilic-thermophilic (M1-T1) AcD and proaches. Agricultural residues such as rice straw, bagasse, wheat straw,
thermophilic-mesophilic (T2-M2) AcD system for biohythane produc cotton stalk, and rice husk could be used for energy conversion pro
tion. They found that a maximum ratio of 8.44% v/v (hydrogen: cesses. For example, Sivaramakrishnan et al. (2021) used biologically-
methane) was obtained from rice straw and pig manure. Additionally, pretreated rice bran for anaerobic hydrogen production using Rhizopus
T2–M2 produced pronounced hydrogen (16.68 ± 1.88 mL/gVS) and oligosporus MTCC 556 strain. They documented that biological pre
methane (197.73 ± 11.77 mL/gVS). This approach could be beneficial treatment using Rhizopus could effectively reduce the 38% lignin
3
Preface Bioresource Technology 331 (2021) 125024
4
Preface Bioresource Technology 331 (2021) 125024
using advanced pyrolysis techniques for hydrogen and gaseous fuel production. Sivaramakrishnan, R., Ramprakash, B., Ramadoss, G., Suresh, S., Pugazhendhi, A.,
Bioresour. Technol. 320, 124299 https://doi.org/10.1016/j.biortech.2020.124299. Incharoensakdi, A., 2021. High potential of Rhizopus treated rice bran waste for the
François, E., Dumas, C., Gougeon, R.D., Alexandre, H., Vuilleumier, S., Ernst, B., 2021. nutrient-free anaerobic fermentative biohydrogen production. Bioresour. Technol.
Unexpected high production of biohydrogen from the endogenous fermentation of 319, 124193 https://doi.org/10.1016/j.biortech.2020.124193.
grape must deposits. Bioresour. Technol. 320, 124334 https://doi.org/10.1016/j. Suksong, W., Kongjan, P., O-Thong, S., 2015. Biohythane production from co-digestion of
biortech.2020.124334. palm oil mill effluent with solid residues by two-stage solid state anaerobic digestion
González-Pabón, M.J., Cardeña, R., Cortón, E., Buitrón, G., 2021. Hydrogen production process. Energy Procedia 79, 943–949. https://doi.org/10.1016/j.
in two-chamber MEC using a low-cost and biodegradable poly(vinyl) alcohol/chi egypro.2015.11.591.
tosan membrane. Bioresour. Technol. 319, 124168 https://doi.org/10.1016/j. Valizadeh, S., Lam, S.S., Ko, C.H., Lee, S.H., Farooq, A., Yu, Y.J., Jeon, J.-K., Jung, S.-C.,
biortech.2020.124168. Rhee, G.H., Park, Y.-K., 2021. Biohydrogen production from catalytic conversion of
Goswami, R.K., Mehariya, S., Obulisamy, P.K., Verma, P., 2021. Advanced microalgae- food waste via steam and air gasification using eggshell- and homo-type Ni/Al2O3
based renewable biohydrogen production systems: a review. Bioresour. Technol. catalysts. Bioresour. Technol. 320, 124313 https://doi.org/10.1016/j.
320, 124301 https://doi.org/10.1016/j.biortech.2020.124301. biortech.2020.124313.
Li, S., Sakuntala, M., Song, Y.E., Heo, J.-O., Kim, M., Lee, S.Y., Kim, M.-S., Oh, Y.-K., Yang, E., Omar Mohamed, H., Park, S.-G., Obaid, M., Al-Qaradawi, S.Y., Castaño, P.,
Kim, J.R., 2021. Photoautotrophic hydrogen production of Rhodobacter sphaeroides Chon, K., Chae, K.-J., 2021. A review on self-sustainable microbial electrolysis cells
in a microbial electrosynthesis cell. Bioresour. Technol. 320, 124333 https://doi. for electro-biohydrogen production via coupling with carbon-neutral renewable
org/10.1016/j.biortech.2020.124333. energy technologies. Bioresour. Technol. 320, 124363 https://doi.org/10.1016/j.
Liu, W., McKibbin, W.J., Morris, A.C., Wilcoxen, P.J., 2020. Global economic and biortech.2020.124363.
environmental outcomes of the Paris Agreement. Energy Econ. 90, 104838 https:// Yukesh Kannah, R., Kavitha, S., Preethi, Parthiba Karthikeyan, O., Kumar, G., Dai-
doi.org/10.1016/j.eneco.2020.104838. Viet, N.V., Rajesh Banu, J., 2021. Techno-economic assessment of various hydrogen
Paillet, F., Barrau, C., Escudié, R., Bernet, N., Trably, E., 2021. Robust operation through production methods – A review. Bioresour. Technol. 319, 124175 https://doi.org/
effluent recycling for hydrogen production from the organic fraction of municipal 10.1016/j.biortech.2020.124175.
solid waste. Bioresour. Technol. 319, 124196 https://doi.org/10.1016/j.
biortech.2020.124196.
Park, J.-H., Chandrasekhar, K., Jeon, B.-H., Jang, M., Liu, Y., Kim, S.-H., 2021. State-of- Sang-Hyoun Kima,*, Gopalakrishnan Kumara,b, Wei-Hsin Chenc, Samir
the-art technologies for continuous high-rate biohydrogen production. Bioresour. Kumar Khanald
Technol. 320, 124304 https://doi.org/10.1016/j.biortech.2020.124304. a
School of Civil and Environmental Engineering, Yonsei University, Seoul
Rajesh Banu, J., Ginni, G., Kavitha, S., Yukesh Kannah, R., Adish Kumar, S., Bhatia, S.K.,
Kumar, G., 2021. Integrated biorefinery routes of biohydrogen: Possible utilization
03722, Republic of Korea
b
of acidogenic fermentative effluent. Bioresour. Technol. 319, 124241 https://doi. Institute of Chemistry, Bioscience and Environmental Engineering, Faculty
org/10.1016/j.biortech.2020.124241. of Science and Technology, University of Stavanger, Box 8600 Forus, 4036
Rambabu, K., Bharath, G., Thanigaivelan, A., Das, D.B., Show, P.L., Banat, F., 2021.
Augmented biohydrogen production from rice mill wastewater through nano-metal
Stavanger, Norway
c
oxides assisted dark fermentation. Bioresour. Technol. 319, 124243 https://doi.org/ Department of Aeronautics and Astronautics, National Cheng Kung
10.1016/j.biortech.2020.124243. University, Tainan City 70101, Taiwan
Renaudie, M., Dumas, C., Vuilleumier, S., Ernst, B., 2021. Biohydrogen production in a d
Department of Molecular Biosciences and Bioengineering, University of
continuous liquid/gas hollow fiber membrane bioreactor: efficient retention of
hydrogen producing bacteria via granule and biofilm formation. Bioresour. Technol. Hawai’i at Mānoa, Honolulu, HI 96822, USA
319, 124203 https://doi.org/10.1016/j.biortech.2020.124203.
Salakkam, A., Sittijunda, S., Mamimin, C., Phanduang, O., Reungsang, A., 2021. Valo *
rization of microalgal biomass for biohydrogen generation: a review. Bioresour.
Corresponding author at: School of Civil and Environmental
Technol. 322, 124533 https://doi.org/10.1016/j.biortech.2020.124533. Engineering, College of Engineering, 50 Yonsei Ro, Seodaemun Gu,
Sarkar, O., Rova, U., Christakopoulos, P., Matsakas, L., 2021. Influence of initial un Seoul 03722, Republic of Korea.
controlled pH on acidogenic fermentation of brewery spent grains to biohydrogen
and volatile fatty acids production: Optimization and scale-up. Bioresour. Technol.
E-mail address: sanghkim@yonsei.ac.kr (S.-H. Kim).
319, 124233. https://doi.org/10.1016/j.biortech.2020.124233.