Experiment No.

5
Carbohydrates
__________________________________________________________________
Name: __Bocalan Ana Maria Bethinna C.__ Date Submitted: ______
Year and Sec: _BSP 3/6_____ Date Performed: _5/10/22___________________
Group No. ________________________ Score: ____________________________

Objectives:

1. To detect the presence of carbohydrate in the different samples using the Iodine
test..
2. To determine which color indicates the presence of carbohydrate in a sample.
3. To discover the effect of temperature and role of saliva in a carbohydrate test.

Theory

Carbohydrates are a major source of energy from our diet. It is composed of the elements
C, H, and O. It is also called saccharides, which means “sugars. Carbohydrates are
produced by photosynthesis in plants. Glucose are synthesized in plants from CO2, H2O,
and energy from the sun. Carbohydrates are oxidized in living cells (respiration) to
produce CO2, H2O, and energy. Monosaccharides are the simplest carbohydrates.
Disaccharides consist of two monossacharides. Polysaccharides contain many
monosaccharides.

Materials

Iodine solution glass jars or vials stirring rod

droppers Starch Sucrose
Brown Sugar Rice w/ Saliva (boiled) Bread w/ Saliva (boiled)
Rice w/o Saliva Rice w/ Saliva Bread
w/o Saliva Bread w/ Saliva

PROCEDURE

Iodine test

1. Place 3 drops of each test carbohydrate solution in separate containers/small

jars
2. Add 1 drop of the iodine solution to each test carbohydrate solution.
3. Note and record the color of each sample
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DATA

Test Sample Observation Result (+/-)

Brown Sugar There is no reaction and change in -

color of the iodine solution.
Starch the iodine solution reacts immediately
with the mixture of cornstarch and
water. The color became blue or in +
purple color

Rice w/o Saliva The Iodine solution reacts on rice. The +

color became more darker like
blueblack in color.
Rice w/ Saliva The saliva change the color of the +
iodine solution on the rice . The darker
color of the iodine solution or the
blueblack color disappear and became
lighter in shade.
Bread w/o Saliva There is a reaction in iodine solution +
on the bread. The color became darker
and became blue black in color.
Bread w/ Saliva The darker color of the iodine solution +
that reacts w/o saliva became lighter
like lighter blue or in purple color.

GUIDE QUESTIONS:

1. What color indicates the presence of carbohydrates in the sample?

The presence of carbohydrate is confirmed by the presence of a purple or violet
ring.

2. What has analysis in the saliva done to the carbohydrate in bread and rice? Be
specific.
Carbohydrate or the Starch gives blueblack color in the presence of iodine
solution. but in
The presence of saliva in carbohydrate, the darker color of iodine solution
disappears and became lighter. As my analysis the saliva help digest starches in
our food. It helps to breakdown all those toxins came from the starch specially the
sugar in carbohydrates you’re eating

3. Is there a different result between boiled and unboiled saliva? Explain.

Illustration:

Brown Sugar Corn Starch Rice Bread

Add 1 drop of Dissolve the Add 1 drop of

corn starch with Add 1 drop
Iodine solution. iodine solution.
water to see the of Iodine
Wait for atleast 1-2 Wait and
reaction and solution
mins to see the observe for
result outcome more atleast 1-2
clearly. minutes to see
the result

Result in
sugar with
iodine Bread with iodine
Result in rice solution and saliva
Add 1 drop of
with iodine
iodine solution
and saliva
in cornstarch
mixture and stir
it well to see the
result.

Result of saliva in
bread with iodine
solution
Result
Generalization:

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