Peroxide Value, Acetic Acid, Isooctane Method Official Method Cd 8b-90

Reapproved 2017
1. REAGENTS
1.1 Acetic acid-isooctane solution :-3:2, v/v
 prepared by mixing 3 volumes of reagent-grade glacial acetic acid with 2
volumes of reagent-grade isooctane.
1.2 Potassium iodide (KI) saturated solution:-,
 prepared fresh each day analysis is performed by dissolving an excess of KI
in recently boiled distilled water. prepare a fresh solution.
1.3 Sodium thiosulfate (Na2S2O3·5H2O) solution 0.01 M
 Standard Solution
1.4Starch indicator solution:
 prepared by making a paste with 1 g of starch and a small amount of cold
distilled water. Add, while stirring, to 200 mL of boiling water and boil for
a few seconds. Immediately remove from heat and cool. the starch solution
should be stable for about 2–3 weeks.
1.5Sodium dodecyl sulfate (SDS)
 Prepare 10% solution by dissolving 10 g SDS in 100 mL water.
2. Procedure
2.1Weigh about 5g of test into a 250 mL Erlenmeyer flask with glass stopper and
add 50 mL of the 3:2 acetic acid-isooctane solution. Swirl to dissolve the test
portion.
2.2Add 0.5 mL of saturated KI solution using a suitable volumetric pipet.
2.32. Allow the solution to stand for exactly 1 min, thoroughly shaking the solution
at least three times during the 1 min.
2.4immediately add 30 mL of distilled water.
2.5Add 0.5 mL of 10% SDS and then add about 0.5 mL of starch indicator solution
2.6immediately titration with constant agitation,especially near the end point, to
liberate all of the iodine from the solvent layer. Add the thiosulfate solution
dropwise until the blue color just disappears.
2.7blank titration must not exceed 0.1 mL of the 0.1 M sodium thiosulfate solution.
 titration with0.1 M sodium
thiosulfate Titrate until
the blue color just
disappear
 Add 0.5 mL of 10%
SDS
Add 0.5 mL of saturated  Add 0.5 mL of
KI starch indicator
solution
immediately add 30 mL
Wt about 5gof of distilled water.
sample+50ml of solvent

Stand for 1min

(1) (2) (3) (4) (5) (6)