Products ps1 280m Apso 6xs0mL KULLANIM AMACL Insan serumundaki Albumin miktarnin in vitro olarak beller METODOLOJI Bromkresol yegil Kontrlld bir pH ainda albUminle ren engin 630 nm deki yogunlugu numunedeki amin = et & bo ALBUMIN ALBUMIN fea Soe ee See ces {sive ey. fae agen ts ce = tah tame re a aid one ek aan og S RETR area Webster tarafindan éne SUrbidugn gibi anik baslangig absorbansielde edi. Pronsip: Kontrol pH BCG + Albumin ———""> Ye REAKTIFIN BILESWI Bromkresol yes ‘Soksinat tampon Surfaktan pH420201 025 mM ‘85mm UYARILAR: 41. Sadece in vito teghis icin kullanii. 2 halinde bulagan yeri tamamen suyla ykayin, 3. Reaktite koruyucu olarak sodyum azidi bulunmaktadir BCG/AIbumin bles Pipeti agzinzia kullanmayin, Cit ve gozlere temasincan sakinin, Temas Kuru haldeyken bakir veya kurgun ie reaksiyona giip patlayici metal aztlen olusturabli. Elden {gkanidiginda, azit birkimini oniemek amacryla ol miktaréa su ile yikayin 4 11915 Git tahrigine yol agar 11319 Clad gz tatrisine yo! agar FReaklifkutu Gstundeki son kullanma tarihinden sonra kullanmayin| 'P302+P352 Deri le temas halinde bol sabun ve sue yikayiniz 'P305+P351+P.398 Gozle temasi halinde suyla brkes dakika durulayrnz. Tekh ve ‘yapmasi kolaysa kontakt lenslen ¢kanin2 Ourulamaya devam odin. (Uygun koruyueu ekipman kullanin REAKTIFIN HAZIRLANMASI Reakif kullanima hazed REAKTIFIN DEFOLANMAS! Reakif oda sicakliginda muhata REAXTIFIN BOZULMASI ‘s09:dai DuranaréaReakih Kulanmayn kif tortulagmss ise, ein (2-25'C) 2 Re ‘ORNEK ALINMASI VE SAKLANMASI Ornek alma: koruyuculan gerekl desi ‘Numune : Serum tavsie edilen omektir if parametreler performans parametrelernikarilamiyor ise HHastanin ozel olarak haziranmasi gerekmez ve numune Depolama :Serum icindeki albumin 28°C de bir ay dayanr. INTERFERAN ETKI 4. Hemoglobin, bilirubin, ipemi ve askorbk asin i belilemek Gzere incelemeler yapiimig ve agagidaki son Hemogiobi Interfaran_seviyelerni usiar eke edimisti: |Hemoglobinin belirgin interteranetksi 1000 mg/dL. ye kadar soz konusu dedi. Bilirubin: Bilirubin berg interferanetkisi 14.8 mg/dL. ye kadar soz konusu dedi Lipemi: 930 mg/GL'ye kadar olgolmus Triglserdd seviyesinde belgin lipemi ‘etkisi soz Konusu del. interteran 2._ Bir dei ilar ve reaktiler Albuminin dogrulugunu etkileyebile GEREKLI OLUP TEMIN EDILMESI GEREKEN ILAVE DONANIM 4. 37°C sabitsicakiija sahip 630 nm’ de absorbans olgebilecek bir kink kimya ‘analzb. 2, Demirden arinmis de iyonize su ve igi donanim, bmegin pipeter. 3. Analizorun igi sar matzemelen, omegin numune ve okuma kaplan. 4 Kontrol ve kallorator materyalle PROSEDOR Dalga Bou 630mm ‘Sicakie arc. Optik Yor om Test Ti Endpoint (son nokta) Reaksiyon Yond Artan utr, ter ezonpanyingdocurents Sentence dakar bey A ® Bega ak, Sian BILIMSEL TIGBI URUNLER PAZARLAMA SANAYI VE TICARET LIMITED SIRKETI ‘ou El Mah 212 Sk. No: Gasiemir-IZMIR- TURKEY + Tt +90 (252) 25260 83 + * ww Diese com Hy t vw products com rm gn pees cann ona apse in enrarmer ewrayntomay opens ve standart absorbansiari okuyun. Prosediir Notlan 4. Mahlelif cinaz gereklernin yerine getiimest hacimler oranti olarak artuniabil. 2. Reaksiyon sicaki kei ofmamakla birkte sical 3. Biri donogamd -gidL x 10= git. HESAPLAMA (Abs= Absorbans ) reakif ve numune ik sabi tutulmatiir, ‘Abs Numune x Standart konsantrasyonu = ALBUMIN (gL) ‘Abs Standart (iat) KISITLAMALAR: 6.0 g/4L. Uzer dederdeki numuneler 1:1 serum fi {ekrar galgimali ve sonuclar ki Be garpilmaliar. KALIBRASYON ‘Albumin standard veya kalibratora kullantr KALITE KONTROL FReaksiyonun boturiog0, belt albumin degerter bul kontrol kulanimiylatakip ediimelir. NORMAL DEGER ARALIGI 35-509! Her laboratuann kendi nownallimiterniolusturmas: PERFORMANS LINEERITE: Tavsiye ediigi gekide caligtiginda, oleam 6 0 gi KIYASLAMA: Bu prosedur le benzer bir metodoloji arasinda ‘as0gidaki sonucian vermistr yolni k le sulandiniip ki seviyeli bie ie ed. Lye kadar tineerdi. lyapilan_incelemeler Mit sseintaye a3 ‘Xin tapers” E~s0 1 Shdognkae Baer en oa ides oom Segre Gulgmaleminde — Svst Save 2 5 Srna ty on ab oo ge) oat ok bv 00 ‘2 : Total ‘Sevivet (N=40) Sraane oy an 2 so ee oe 3 Bae tf 3 FnaSabive. tap 735 itlron teeth bf. bn oSaerorowesnnee es BILIMSEL KAYNAKCA teed eed ees eeatiemen neers eee Nesaze Aun Goet in Gram 23653 1 Baus BY rnn Wm ord ge NG 191 ein Sanaa nd he masereners {Sn Abuma ain Berne Groen On Chow hla 3137 «Yong 08 ect Dugan Ciel abrtoy Toa Prd Eton Manutacturer/ Ure Bilge! bb Ort Fax 990 (232) 250 Intoaaimeatp ce 192026 52 Son Ts Lid Sb. pom crmee ce€eLBUMIN ALBUMIN products ne re fm [Re za ABI Gainh BION tntankABTOICID Sak . oe Ges Gee ft {ame ~ wvreNoeo use ‘lank Sta Sample "For the vito quantitative detemination of albumin concenraon in sean Reagent 000. TOOL TODD aL ) METHODOLOeY Distites Ou : 5 Ata controled pt, bromeresl green foms a colored complex wi abumin. The | water intenaty of eotr at 850 nt diealy proportional e aaanin corte The Standara = spat - itartneous ill absorbance olaned as suggested by Webster Sample 3 103. 2) Prinetpies * Gontotes pH -BCG+ ALBUMIN = ———® Green BCG/ALBUMIN Complex ‘Mix and then incubate for 90 second at 37°C. Mgasure the absorbance of REAGENT COMPOSITION sample and stand again the reagert lark Active Inaredients — Procedure Notes ‘romereso green ml 4 Tne reagent and sample volves maybe ated proportonay 10 Succinat Bufor ‘5 mM eekressn eren esiurnerts users eanvos, 2. The temperature of the reaction is not critical the tomperture snoué be hed constant PRECAUTIONS re 4. For in vitro diagnostic use only. 2 DO NOT pipette by mouth.” Avoid contact with skin and eyes. spit, thoroughly, wash affected aress wih water. a Reagent contains Sodium Azde as a preservative. This may react with copper or lead plumbing to form explosive metal azides. Upon disposal, fush with large amounts of water to prevent azide build up. Do not use the reagent after the expiration date printed onthe kit 4 J 11315 Causes skin imation 11319 Causes serious eye tation 'P302+352: IF ON SKIN: Wash with soap end water £P305+P3514P338 F IN EYES ‘Rinse continuously with water for overs! ‘minutes Remove contact lenses if present and easy to do Continue rinsing Wear suitable protective equipments )) REAGENT PREPARATION Reagent is supplied readyto use. « REAGENT STORAGE Store reagent at room temperature. (2-25°C) REAGENT DETERIORATION The reagent should not be used 1 2 ‘The reagent is turbid “The reagent is to meet stated parameters of performance ‘SPECIMEN COLLECTION AND HANDLING. 'No special preparation ofthe patent is necessary and sample preservatives are not required Serum, is the recommended specimen, ‘Sample Storage: Albumin in serum is stable for one month at 2-8°C. INTERFERENCES. Collection: Sample Type: 3 Hemoglobin: ‘Studies to determine the evel of interference for hemoglobin, blrubin, and lipernia were carted out, the following results wore obtained: No significant interference from hemoglobin up to 1000 mg/d Bilin: No significant interference from bilirubin up to 14.8 mg/dl. Lipemia: 'No signffcant interference from ipemia up to 930 mgidl. measured as triglyoerides, 2. ‘A number of drugs and substances may affect the accuracy of Albumin, ADDITIONAL EQUIPMENT REQUIRED BUT NOT PROVIDED: 4 ‘A clinical chemistry analyzer capable maintaining constant temperature (B7°C), and measuring absorbance at 830nm, 2 3. a PROCEDURE Wavelength Working temperature: Optical path Assay type Direction A & Sposa ononetan reiemne Bigs ik Simi Dota yl Mah 3121 Sx N12 Gaza Iron-ftee deionized water and related equipment, e.g: pipettes ‘Analyzer specific consu ‘Control and callbrator materials ‘eg. sample and read cups 630 nm are tom Endpoint Inereasing ft BILIMSEL TIBBI URUNLER PAZARLAMA SANAYI VE TICARET LIMITED SIRKETI + Tel 490 232) 262 60 9 « vwnmipeducts com: » naw bap lawl TUR ona nace Karon pres ohn esr tory ope 3. Unit Conversion: g/dl x 10 = gf. CALCULATION (Abs = Absorbance) ‘Abs. of Sample_x cone. of std. (gL) = ALBUM Abs. of sandard LIMITATIONS ‘Samples with values exceeding 6.0 g/dl should saline and re-run. The fal answer should be mul CALIBRATION Use an equecus albumin standard or an appropri QUALITY CONTROL (ost) dlluted 1:4 with lied by two, serum calibrator. ‘The integrity of the reaction should be monitored ty use of @ two level control wth known albumin values. EXPECTED VALUES ~ 35-50 gid itis strongly recommended that each laboratory eftablsh its own normal ‘ange PERFORMANCE LINEARITY: When tun as recommended the assay ie linear METHOD COMPARISON: 6.098 ‘Studies performed between this procedure and a gimilar methodoloay yielded the folowing results: Number of samples pais: Range of samples: Comteiation Coefficient Stope: Intercept Precision: Within Run n=40 Mean (g/dL) SD (g/dl) Cv. 0%) Total n=40 Mean (gict) SD (oil) Cv.) SENSITIVITY: AA calibration factor of approximately 7.35. w: ‘equivalent to a sensitivity of 0.136 A Abs per gil. “3 080-5, 0.987 0.988, 3 0 1 aa 288 REFERENCES {Tet Neon of Cia Chama Prscnioha W8 Sa 1 Vicon 17 The mca Racin bates onsen 14 Yeirg 08, Ein true en Cea Labo oo Tod Fax +90 (232) 250 61 23 + hlobiimactip (al 1 LAT 0 8 Manure! Oren ‘limea! Tibbs, Ornle Paz San Tie Us 9b 17 8 6 dL) 2 obtained, which is 108 99 701-704 Sonera 2 » CE