10秒 2滴 3滴 4滴 10分 15分

4 gotas 10 sec. 1滴 1 min. 10 m

10㎕ 20㎕
3 14分钟 1
1 drop 2 drops 3 drops 4 drops
2 gotas 3 gotas
90o 90o 90o 90o

ONE STEP Canine Parvovirus Antigen and Canine Coronavirus Antigen Test 10秒 2滴
drops drops
3滴 drop
4滴 10
Pre-wet swab with
4 gotas 10 sec. 1滴 1 min. 10 min. 15 min
a saline solution
4) Wait for 1 minute to settle down the feces. 20
For veterinary diagnostic use only 2 gotas 3 gotas ㎕
10秒 2滴 3滴 4滴 10分 15分

10 152 gotas 20
Pre-wet swab with

3 4 2 1
a saline solution

Anigen Rapid CPV/CCV Ag Test Kit (3lines) min. min. 3 gotas

min.
滴 滴 滴 滴
1分钟
Pre-wet swab with
a saline solution 10 15 20
5) Remove the test device from the foil pouch, min. min. min.

■■ Principles
10 15 1020 15 20
and place it on a flat and dry surface.
6) Using a disposable dropper, take the
10 15 20
The Anigen Rapid CPV/CCV Ag Test Kit (3lines) is a chromatographic immunoassay min. min. min.
for the qualitative detection of Canine Parvovirus(CPV) antigen and Corona 分钟 in the tube. 分钟
supernatant sample 分钟
分钟 分钟 分钟
1 drop 2 drops 3 drops 4 drops
virus(CCV) antigen in canine feces.
10 15 20
Anigen Rapid CPV/CCV Ag Test Kit (3lines) has three letters which are test line
(“1” and ”2”) and control(C) line on the surface of device. Test line and control line 分钟 分钟 分钟
in the result window are not visible before applying any samples. Test line and 4 gotas 10 sec. 1滴 1 min.
control line in the result window are not visible before applying any samples. The 30
3 4
into the 3 4 1
control line is a reference line which indicates the test is performing properly. The 90o 90o 90o 90o

7)90Add four (4)90 drops of sample drops drop

10秒drops 2滴 drop 3滴 4滴
90o o
90 o o

control line has to appear every time when the test has performed. The control drops
3 4 120
line has to appear every time when the test has performed. The highly selective sample hole, drop by drop vertically.
90o 90o 90o 90o

20
selected antibodies to CCV and CPV are used in each test line(“1” and ”2”) as a drops drops drop㎕

2 gotas 3 gotas

CPV/CCV Ag
capture and detector. These antibodies are capable of detecting CPV antigen and
3 4
㎕ 20
CCV antigen in canine feces with high accuracy. Pre-wet swab with ㎕

3 4 2 1
a saline solution 滴 滴
8) Start the timer. The sample will flow across
■■ Materials provided (10 Tests/Kit) the result window. If it does not appear 30 3 4 2 1
滴 滴 滴 滴

10 15
滴 滴 滴 滴

30
after 1minute, add one more drop of
Materials 10 Tests/Kit
prepared sample to the sample hole.
Anigen Rapid CPV/CCV Ag Test device (3lines) 10 min. min.
Assay diluent tube 10 9) Interpret test results at 10 minutes.
Disposable swab 10 Do not read the result after 20 minutes.

10 15
Disposable dropper 10
Instructions for use 1
■■ Interpretation of the Result 分钟 分钟
1) Negative result
■■ Materials required, but not provided Only control(“C”) line in the result window appears.
Timer

CPV/CCV Ag
■■ Precautions
1) The test kit is for canine use only. Do not use for other animals. 90o 90o 90o 90o 3
2) Positive result dro
2) The test device is sensitive to humidity as well as heat. Perform the test
immediately after removing the test device from the foil pouch. Control(“C”) line and test(“1” and/or “2”) line in the result window appears
3) Do not reuse the test components. indicate the presence of CPV antigen and/or CCV antigen.
4) Apply the sample using disposable dropper vertically. CPV Ag Positive ("C" and "2") CCV Ag Positive ("C" and "1")
30
3
5) Do not touch the membrane in the result window of test device.
30
CPV/CCV Ag

CPV/CCV Ag
6) Do not use the test kit beyond the stated expiration date marked on the
package label. 滴
7) Do not use the test kit if the pouch is damaged or the seal is broken.
8) Do not mix components from different lot numbers because the components Simultaneous CPV Ag and CCV Ag Positive ("C", "2" and "1")
in this kit have been quality control tested as standard batch unit.
CPV/CCV Ag

9) All samples should be handled as being potentially infectious. Wear protective

30 hands thoroughly afterwards.
gloves while handling samples. Wash
10) Decontaminate and dispose of all samples, reaction kits and potentially
contaminated materials safely in accordance with national and local regulations. 3) Invalid Result
If the control(“C”) line does not appear after 10 minutes, the result might be
■■ Storage and Stability considered invalid. The sample should be retested.
1) Store the test kit at 2~30°C. DO NOT FREEZE.
CPV/CCV Ag

CPV/CCV Ag

2) Do not store the test kit in direct sunlight.

3) The test kit is stable within the expiration date that marked on the package label.

■■ Collection and Preparation of Sample

CPV/CCV Ag

CPV/CCV Ag

1) Canine feces swab should be used for this test.

2) The samples should be tested immediately after collection.
3) If samples are not tested immediately, they should be stored at 2~8°C for 24
1 drop 2 drops 3 drops 4 drops 10㎕ 20㎕
hours. For longer storage, freeze at -20°C or below. Frozen samples should be
brought to room temperature (15~30°C) prior to use. ■■ Limitations of the Test
4 gotas 10 sec. 1滴 1 min. 10 min. 15 min.
4) The amount of fecal swab may affect the results. It is required to follow the 1) Although the Anigen Rapid CPV/CCV Ag Test Kit (3lines) is very accurate in
swab amount of feces as shown in the picture on the below side. Excessive detecting CPV antigen and CCV antigen, a low incidence of false results can
10秒 2滴 3滴 4滴 10分 15分
fecal amount may induce a false positive result and slow migration. occur. Other clinical and/or laboratory tests might be required if questionable
3 gotas 10㎕ 20㎕
1 drop
results are
2 drops
obtained. As other
3 drops2 gotas4 drops
diagnostic tests, a definitive clinical diagnosis
1分钟
should not be based on the result of a single test, but should be diagnosed by
30
Pre-wet swab with
a saline solution
the veterinarian after all clinical and laboratory findings have been evaluated.
4 gotas The10limit 1滴
sec. of detection 1 min.
for CPV Ag10 is min. 15 min. 3.13x105TCID50/ml and for
approximately
CCV Ag is approximately 1.97x10 10 4TCID50/ml.15 20
min. min. min.
10秒 2滴 3滴
2) The reading window may 4滴 10分
show a light 15分
pink background coloration; this will not
affect the accuracy of the results.
Not enough Good Good Too Much 3 gotas 10 15 1分钟 20
3) BioNote and its distributors cannot be held responsible for the consequences
2 gotas
Pre-wet swab with
of misuse or misinterpretation分钟of the results 分钟 given by分钟 the test.
a saline solution

■■ Procedure of the Test

1) All reagents must be at room temperature (15~30oC) before use.
10 15 20
2) Collect feces samples by using a swab. min.90 min. min. 3 4 1
10㎕ 20㎕
o
90o 90o 90o

1 drop 2 drops 3 drops 4 drops

drops
drops drop

20

1010 1滴15 1 min.20 10 min.

or ㎕

4 gotas sec. 15 min.

分钟 分钟 分钟
3 4 2 1
1 drop 2 drops 3 drops 4 drops 10㎕ 20㎕ 滴 滴 Doc.滴No.: I1113-4E
滴

10秒 2滴 3滴 4滴 10分 15分

Revised date: Jun. 08, 2021
3) Insert the swab into the assay diluent 4 gotas 10 sec. 1滴 1 min. 10 min. 15 min.
tube and mix the swab until the sample
2 gotas 3 gotas 1分钟
has been dissolved into the assay diluent
Pre-wet swab with
10秒 2滴 3滴
90o 4滴
90
o
10分 90o 15分 90o 3 4 1
(approximately 10 seconds). drops drops drop
a saline solution

2 gotas 3 gotas 1分钟

20

10 15 20
Pre-wet swab with
a saline solution ㎕