IVD in vitro diagnosticum - For professional use only

Anaerotest®
Anaerotest® Cat. No. 1.15112.0001
(50 Strips)

For the detection of an anaerobic atmosphere

See also General Instruction of Use

Warnings and precautions see www.merck-chemicals.com

Principle
Microbiological method

Mode of Action.
The blue oxidized form of the dye methylene blue is converted in oxygenfree (anaerobic) medium into the
(colourlesss) leucomethylene blue. In the presence of oxygen the reduced leucobase passes again into the
oxidized form (blue).

Typical Composition (g/litre)

Methylene blue - Reducing agent - Stabilizer.

Preparation and Storage

Cat. No. 1.15122.0001 Anaerotest®
Store dry and tightly closed.
Store at +15 °C to +25 °C.
Only remove the number of sticks required at the time!
Do not touch the reaction zones of the test sticks!
Close containers tightly again at once

Experimental Procedure and Evaluation

The indicator is used together with Anaerocult® A (Cat. No. 1.13829.) in the anaerobic jar, with Anaerocult® A
mini (Cat. No. 1.01611.) for 1-4 Petridishes and with Anaerocult® P (Cat. No. 1.13807.) using just one Petridish.
Anaerocult® IS (Cat. No. 1.16819.) is placed in the special incubation bag together with the agar identification
system or 1-2 microtitre plates and the anaerobiosis indicator Anaerotest®. Of course Anaerotest® can also be
used with other anaerobiosis systems.

Moisten the reaction zone with one drop of distilled water and put Anaerotest® into the anaerobic jar.
When using Anaerocult® (Cat. No. 1.13829.), Anaerocult® IS (Cat. No. 1.16819.), Anaerocult® A mini (Cat. No.
1.01611.) and Anaerocult® P (Cat. No. 1.13807.) draw exact instructions from the respective package inserts.

© Version 2010-11-02 Merck KGaA, 64271 Darmstadt, Germany, tel. (06151) 720, www.merck.de