Types of Blanks - A secondary standard is a

substance whose active

 equipment blank
agent contents have been
 field blank
found by comparison
 trip blank
against a primary
 method blank
standard.
 instrument blank

PURPOSE Kinds of STANDARDS:

is to trace sources of
- A primary standard is a
artificially introduced
reagent by which an
contamination. It is used to
calibrate the readings of appropriately known
spectrophotometer: they amount of analytes can
document the baseline response be dispensed.
of the environment-instrument- - A primary standard must
sample system. It is analogous have a known
to “zeroing” a scale before stoichiometry, a known
weighing, allowing you to purity or assay, and it
BLANK

document the influence of the must be stable during

particular instrument on your long-term storage.
readings.
Blank solution will contain - A control tube assures
everything that the 'unknown' the experiment is
solution (the one you want to working properly such as
measure) except for the think adding the final product
you wish to measure. The to your solution without
'blank' allows you to set the the precursors.
spectrophotometer to zero - Control is a solution of
before you measure your known composition that
'unknown' solution. is analyzed along with
 is to ensure the accuracy and test samples in order to
CONTROLS

precision of the measurement, evaluate the accuracy of

it is necessary when trying to an analytical procedure.
quantify the concentration of an It is also known as check
unknown. Having a standard sample.
helps minimize instrument and
STANDARD

reagent error as well as user Types of Control:

error. - The power switch doubles
 Standards are materials as the zeroing control.
containing a precisely known - Light control regulates
concentration of a substance the amount of light
for use in quantitative passing through the exit
analysis. A standard provides a
slit.
reference that can be used to
Wavelength control transmits
determine unknown
only the desired wavelengths.
concentrations or to
calibrate analytical instruments.
 - The test or unknown tube
is the one whose
unknown concentration is
going to be tested in
spectrophotometric
analysis. It is labeled test
TEST or UNKNOWN

or unknown because it is
the one who is going to
be tested and the goal of
the test is to determine
what is the concentration
of the unknown.
- Test is the solution whose
unknown
concentration is going
to be tested in
spectrophotometric
analysis.

Absorbance Color
values
500nm Red
wavelength
700 nm Green- Blue
wavelength
340nm Ultraviolet
wavelength
410nm Yellow-Green
wavelength
850nm Infrared
wavelength
620 nm Blue-Green
wavelength