PRACTICAL GUIDELINE

INTRODUCTION TO ANALYTICAL CHEMISTRY

LECTURER (S): ELIYAWATI, M.PD

QUALITATIVE ANALYSIS
A. OBJECTIVES C. MATERIALS
F

To identify ion (cations and anions)

To write some of chemical reactions

B. EQUIPMENT
Test Tubes (12 including Pyrex)
Test Tube Holder (1)
Dropping pipette (6)
Nichrom Wire (1)
Spiritus burner (1)
12 Well-Plate (1)

D. BASIC THEORY

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 E. PROCEDURE
PROCEDURE OBSERVATIONS
1. Flame Test
• Immerse the cleaned nichrom wire F
into Hydrochloric Acid concentrate.
• Put into the bunsen flame (in the
oxidation flame)
• Do the 2 step 2x
• Immerse the cleaned nichrom wire
into Hydrochloric Acid concentrate.
• Touched into the sample (sample
is placed on 12-well plate)
• Put into the bunsen flame
• Observe the flame color

2. Identify Ag(I) ion

Test tube 1
• Take 1 ml of Silver Nitrate 0,1 M
• Add Hydrochloric Acid 2 M drop
by drop
• Dissolve the precipitation using
Ammonia 0,1 M until its changed

Test Tube 2
• Take 1 ml of Silver Nitrate 0,1 M
• Add Potassium Chromate 1 M
drop by drop

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 PROCEDURE OBSERVATIONS
3. Identify Pb(II) ion
• Take 1 ml of Lead(II)Nitrate 0,1 M F
• Add Hydrochloric Acid 2 M drop
by drop
• Heat it with small fire

4. Identify Ag(I) ion

• Take 1 ml of Copper Sulphate 0,1 M
• Add Ammonia 3 M drop by drop
• Continue adding Ammonia until its
changed

5. Identify Fe(III) ion

• Take 1 ml of Iron(III)Chloride 0,5 M
• Add Sodium Hydroxide 1 M drop by
drop

6. Identify Ba(II) ion

Test Tube 1
• Take 1 ml of Barium Chloride 0,1 M
• Add Sulphuric Acid 2 M drop by drop
Test Tube 2
• Take 1 ml of Barium Chloride 0,1 M
• Add Potassium Chromate 1 M drop by
drop

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 PROCEDURE OBSERVATIONS
7. Identify Acetate Ion
• Take 1 ml of Sodium Acetate 0,1 M F
• Add Hydrochloric Acid 6 M drop
by drop

8. Identify Nitrate Ion

• Take 1 ml of Potassium Nitrate 0,1
M
• Add 1,5 mL of Iron(II)Sulphate 1 M
(fresh solution)
• Pour 1.5-2.5 mL of Sulphuric Acid
concentrated slowly through the wall
of test tube until its formed a layer.

9. Identify Oxalate Ion

• Take 1 ml of Sodium Oxalate 0,1 M
• Add Acetic Acid 2 M 5 drops
• Add Calsium Chloride 1 M drop by
drop

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F. ANALYSIS DATA (INCLUDING CHEMICAL REACTION)

G. CONCLUSION

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