VII International Congress “Engineering, Environment and Materials in Process Industry“

BRIDGING THE GAP BETWEEN SUSTAINABILITY AND DYE REMOVAL

PROCESSES: DYE BIODEGRADATION BY PEROXIDASE FROM
AGROINDUSTRIAL WASTE

Milica Svetozarević1,*, Nataša Šekuljica1, Ana Dajić1, Marina Mihajlović1, Zorica Knežević-
Jugović2, Dušan Mijin2
1
Innovation Center of the Faculty of Technology and Metallurgy, Karnegijeva 4, 11000 Belgrade,
Serbia, msvetozarevic@tmf.bg.ac.rs
2
University of Belgrade, Faculty of Technology and Metallurgy, Karnegijeva 4, 11000 Belgrade,
Serbia

Abstract

Textile manufacturing is among the industries with the highest adverse environmental impact as
the greatest consumers and contaminants of water. One of the main pollutants are dyes used in
the coloring process, which concentration in effluents range 10-200 mg/L. The spent textile dyes
released in wastewater can cause pH imbalance, eutrophication, and photosynthesis
deterioration, suppression of growth, development and reproduction of aquatic biota. According
to their chemical structure, dyes can be classified as azo, anthraquinone, nitro, nitroso,
phtalocyanine, acridine, oxazine, triphenylmethane, methane etc. Anthraquinone dyes are the
second most used dyes in textile industry. Their application includes dyeing of silk, wool and
poliamide materials with fine properties such as color fastness, brilliance and thermal stability.
Although their application is widespread, their complex structure of fused aromatic rings makes
them difficult for degradation. Therefore plenty of physical, chemical, biological and combined
methods have been developed. Most common physical methods are based on adsorption with
activated charcoal as adsorbent. Even though its production is simple, this methods leads to
solid waste generation. Chemical method involve mostly the use of harsh chemicals. Methods
including microorganisms are time consuming with sludge generation. Enzymes are an effective
alternative due to the reduction of sludge volume and degradation products that are less toxic. In
anticipation of “greener” and cost effective process, agroindustrial waste may serve as an
enzyme source. In this study, a crude enzyme extract from agroindustrial waste- potato peel and
soybean hull, is obtained for biodegradation of anthraninone dye Acid Violet 109. In order to
achieve maximum biodegradation, the process parameters: contact time, pH, enzyme, hydrogen
peroxide, dye concentration and temperature were optimized. Apart from this, kinetic
parameters are the key factors for designing an efficient process. With the kinetic parameters,
the right enzyme or enzyme source can be selected. Eventually, it leads to substantial
acceleration of the process with minimal inhibition of the biocatalyst. Thus, the initial kinetics of
the given enzymes was inspected and later the experimentally acquired values were modelled by
the kinetic of bisupstrate reactions.

Key words: peroxidase, agroindustrial waste, dye treatment, ping pong bi-bi, Acid Violet 109