16.14.21 rial from accumulating on wall of flask above surface of liquid.

AOAC Official Method 969.44 Immediately add 100 mL 1 + 1 mixture Tween-80 plus 60% alcohol
Light Filth in Oregano (Unground) solution, 945.75C(w) (see 16.1.01), and Na4EDTA plus alcohol so-
Flotation Method lution, 945.75C(y) (see 16.1.01), and swirl few seconds, again using
First Action 1969 plunger to clear material from wall of flask. Let stand 10 min in cold
Final Action 1996 H2O bath. Dilute to 800 mL with 15% alcohol. Add 50 mL mineral
oil, 945.75C(p) (see 16.1.01), and stir magnetically, 970.66B(c)
A. Reagent
(see 16.1.02), 2 min. Fill with 15% alcohol and hand stir every
15% Alcohol.—Prepare ca 1700 mL/test portion prior to analysis. 2–3 min for 20 min. Clamp stirring rod in place so that plunger is
held above sediment at bottom of flask. Leave flask undisturbed
B. Determination
10 min. Trap, filter onto ruled paper, and examine microscopically.
Weigh 10 g test portion into 2 L trap flask, 945.75B(h)(4) (see
Reference: JAOAC 52, 21(1969).
16.1.01), add 400 mL 60% alcohol, and boil gently 10 min, occa-
sionally swirling flask gently and/or using plunger to prevent mate- Revised: March 1997

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