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CODE 80392 REV 0 DEC 2019 (It can be modified without notice)
SOFTWARE
M.WAVE PROFESSIONAL
V-1100 / VR-2000
USER MANUAL
(FOR SPECTROPHOTOMETERS
V-1100 & VR-2000)
4312001
J.P. SELECTA s.a.u. Autovía A-2 Km 585.1 Abrera 08630 (Barcelona) España Tel 34 937 700 877 Fax 34 937 702 362
e-mail: selecta@jpselecta.es - website: http://www.jpselecta.es
Contents
Functions ............................................................................................... 1
Starting.................................................................................................. 3
PC System Requirements............................................................. 3
Introduction ........................................................................................ 7
Operation ............................................................................................. 13
Concentration Measurement................................................ 17
Functions
This section introduces the functions of the UV-Vis
Analyst.
Main Functions
Single wavelength photometric measurement
Go to a desired wavelength quickly and conveniently.
Photometric value display mode can be changed (%T or
Abs).
Concentration Measurement
2 methods to set up the regression curve.
Up to 20 standards to set up the regression curve.
The UV-Vis Analyst will calculate the working curve
using a linear equation that fits the data. Enter
factor values to generate regression curve.
3 methods for curve fit.
Linear fit, Quadratic fit and Cubic fit.
Wavelength Scanning
Allow user to set scan step (0.1, 0.2, 0.5, 1.0 and
5.0nm).
Spectrum display mode can be changed (Wavelength-%T
or Wavelength-Abs).
Peaks and valleys will be automatically detected
after scanning (User can define the peak threshold).
Powerful spectrum processing functions are provided.
Time Scanning
1
UV-Vis Analyst
DNA/Protein Measurement
7 methods to measure DNA/Protein.
Wavelength points and ratios can be set up.
Results will be grouped into a table format
automatically.
Scale Expansion
Simultaneous expansion of the X and Y axes are provided
with the “Zoom” function. Display range can also be
changed though the “Display Setup” function.
Differentiation
You can calculate and display the first through to the
fourth derivative spectrum for a given spectrum.
Derivative spectrum is useful for enhancing spectrum
data that are not readily apparent in an absorbance
spectrum.
Calculate Spectrum
You can calculate addition, subtraction, multiplication
and division between two spectrum with the resulting
data displayed on the screen.
2
UV-Vis Analyst
Reset Wavelength
It affords to relocate the 656.1nm.
Starting
This section introduces how to start to use the UV-Vis
Analyst.
PC System Requirements
Pentium or above PC;
CD-ROM;
USB Ports.
512MB Memory(1GB or Above is strongly recommended);
3
UV-Vis Analyst
4
UV-Vis Analyst
5
UV-Vis Analyst
Main Menu
Basic Mode
Quantitative Mode
Wavelength Scan
Kinetics Mode
DNA/Protein Mode
Multi-Wavelength Mode
System Utility
6
UV-Vis Analyst
Introduction
We will introduce the UV-Vis Analyst in this chapter.
Main Interface
This is the main interface after start.
7
UV-Vis Analyst
Data Zone
Status Bar
New a Fixed
Measurement
Wavelength
New a Time Scan
Scan
Measurement
Measurement
8
UV-Vis Analyst
New a
DNA/Protein
New a
Measurement
Instrument
Open… Open a
Validity
spectrum/data
Close Close current
file
measurement
Save Save current
instrument
method
Print… Print test
Status of status
Displaybar
status
View
Spectrophotomet
Status font of
Setup font of
er spectrophotomet
status
Customize Define bar
the
er
information of
9 display and
print
UV-Vis Analyst
Magnify value
Magnify the
Search default
Search
parameters
peak/valley one
Link Connect to the
fdisplay
by one
Spectrophotomet
Reset Instrument
Reset
er
Spectrophotomet parameters of
Escape Stop current
er instrument
measurement
View dark Retest the dark
UV-
Current
Set Amplifier current
Reset amplifier
Photomete
Locate 656.1nm Relocate
r
Calibrate 656.1nm
Scan system
System Baseline
Automatic Blank baseline
Do blank
Calibration
Slit Bandwidth Set slit
*
Set Unit bandwidth
Set unit (0.5,
10
UV-Vis Analyst
lamp
Turn on/off D2 lamp
Turn on/off D2
lamp
D2/W Switch lamp
Set switch
Point
Comm. Port point
Setup of D2/W
comm.
Setup
Change Password port
Set/Change
Start samples
Start a
automatically
measurement
Scan Stop Stop a
Service measurement
Measure
parameters
peak/valley
Add Add two
Compute
threshold
spectrum
Sub Subtract one
spectrum from
11 another
UV-Vis Analyst
Divide spectrum
Divide one
Averaging another
spectrum
Savitzky-Golay Smooth a with
Filter Moving
the Window
method
Resample spectrum
Resample a
Averaging
Savitzky-Golay
New Window spectrum
New a
Smoothing
Cascade measurement
Multi windows
Filter
window
display as
in a
Window Tile Multi windows
current
cascade
Arrange Icons display
Arrange in
alla
tile
icons
Split Split minimized
display
Analyst information
Setup
Vis Analyst
parameters
measurement
Delete results
result
selected
12
UV-Vis Analyst
one wavelength
Display
Instrument CPU
Delete current
information
Spectrum
Display result
as mode %T
Display result
as mode
Undo Abs
Scale
Operation
This chapter introduces how to use UV-Vis Analyst.
13
UV-Vis Analyst
190-1100nm.
3. Do blank.
Single Beam: Place a reference in the
compartment, click Zero.
Double Beam: Skip to step 4.
4. Measure Sample.
Single Beam: Place a sample in the sample
compartment.
Double Beam: Place a reference in the reference
compartment and place a sample in the sample
compartment.
The photometric value will display in the Readout
box.
14
UV-Vis Analyst
15
UV-Vis Analyst
6. Do blank.
Single Beam: Place a reference in the
compartment, click .
Double Beam: Skip to step 7.
7. Measure Sample.
Single Beam: Place a sample in the sample
compartment.
Double Beam: Place a reference in the reference
compartment and place a sample in the sample
compartment.
16
UV-Vis Analyst
Concentration Measurement
Set Up Linear Regression Curve
There are two methods available to set up the linear
regression curve. You can use standards to set up the
regression curve or just key in the parameters
manually. Use the following steps to select the method
you wish to use.
17
UV-Vis Analyst
compartment, click .
Double Beam: Skip to step (4).
(4) Measure Standard Sample.
Single Beam: Place a sample in the sample
compartment.
Double Beam: Place a reference in the reference
compartment and place a sample(Standard 1) in
the sample compartment.
Click Start to run a measurement.
(5) Key in the concentration value of Standard 1 in
the Conc. box.
(6) Key in the sample name for the standard in the
Name box.
(7) Click OK. The photometric data, △A and
concentration will be shown in the standard table.
(8) Repeat steps (4)-(7) to measure all the prepared
standards.
18
UV-Vis Analyst
19
UV-Vis Analyst
20
UV-Vis Analyst
compartment, click .
Double Beam: Skip to step 4.
4. Measure Sample.
Single Beam: Place a sample in the sample
compartment.
Double Beam: Place a reference in the reference
compartment and place a sample in the sample
compartment.
21
UV-Vis Analyst
Wavelength Scanning
This chapter describes how to collect a spectrum while
using Wavelength Scan function.
Scan Sample
22
UV-Vis Analyst
23
UV-Vis Analyst
5. Scan baseline.
Single Beam: Place a reference in the
compartment, click .
Double Beam: Skip to step 6.
6. Measure Sample.
Single Beam: Place a sample in the sample
compartment.
Double Beam: Place a reference in the reference
compartment and place a sample in the sample
compartment.
24
UV-Vis Analyst
25
UV-Vis Analyst
Rescale
Original Scales
26
UV-Vis Analyst
27
UV-Vis Analyst
Trace a Spectrum
28
UV-Vis Analyst
Derivative
29
UV-Vis Analyst
30
UV-Vis Analyst
31
UV-Vis Analyst
Resample
32
UV-Vis Analyst
Spectrum Addition
Spectrum addition can assist in the development of
artificial spectrum in multi-component mixtures.
33
UV-Vis Analyst
Spectrum Subtraction
Subtracting one spectrum from another has been a
classical technique to offset spectrum interference
from the spectrum of interest.
34
UV-Vis Analyst
Spectrum Multiplication
Multiplying spectrum can assist in the development of
artificial structure of spectrum in multi-component
mixtures.
35
UV-Vis Analyst
Spectrum Division
Dividing one spectrum from another has been a classical
technique to offset spectrum interference from the
spectrum of interest.
36
UV-Vis Analyst
Unload a Spectrum
Select the spectrum you want to unload as the Current
37
UV-Vis Analyst
Scan Sample
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UV-Vis Analyst
4. Do blank.
Single Beam: Place a reference in the
39
UV-Vis Analyst
compartment, click .
Double Beam: Skip to step 5.
5. Measure Sample.
Single Beam: Place a sample in the sample
compartment.
Double Beam: Place a reference in the reference
compartment and place a sample in the sample
compartment.
by clicking .
Calculate Rate
40
UV-Vis Analyst
type the end time in Time End box, and type the K
factor in K Factor box, click Calculate, the result
will be displayed.
DNA/Protein Measurement
This chapter describes how to perform DNA/Protein
41
UV-Vis Analyst
measurement.
DNA/Protein Measurement
42
UV-Vis Analyst
4. Do blank.
Single Beam: Place a reference in the
compartment, click .
Double Beam: Skip to step 5.
5. Measure Sample.
Single Beam: Place a sample in the sample
compartment.
Double Beam: Place a reference in the reference
compartment and place a sample in the sample
compartment.
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UV-Vis Analyst
44
UV-Vis Analyst
Appendix
Methods of Quantitative Analysis
Single Wavelength Method :Abs.=A1
Double Wavelengths Method :Abs.=m*A1-n*A2
Three Wavelengths Method :Abs.=A1-(WL1-WL2)*(A2-
A3)/(WL2-WL3)-A3
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UV-VIS ANALYST FOR SPECTROPHOTOMETER