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    bhuneshwar paswan
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    This document provides a recipe for making PAS (Page's Amoeba Saline Solution), which is used as a constituent in protozoa media. It lists the constituents needed to make stock solutions 1 and 2, and then the amounts of each stock solution needed to make 1 liter of the final PAS medium. The medium is prepared by adding 5 ml each of stock solutions 1 and 2 to deionized water, then autoclaving and making up to 1 liter total volume.

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    MR_PAS

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    This document provides a recipe for making PAS (Page's Amoeba Saline Solution), which is used as a constituent in protozoa media. It lists the constituents needed to make stock solutions 1 and 2, and then the amounts of each stock solution needed to make 1 liter of the final PAS medium. The medium is prepared by adding 5 ml each of stock solutions 1 and 2 to deionized water, then autoclaving and making up to 1 liter total volume.

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    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Flag for inappropriate content
    19 views1 page

    MR Pas

    Uploaded by

    bhuneshwar paswan
    This document provides a recipe for making PAS (Page's Amoeba Saline Solution), which is used as a constituent in protozoa media. It lists the constituents needed to make stock solutions 1 and 2, and then the amounts of each stock solution needed to make 1 liter of the final PAS medium. The medium is prepared by adding 5 ml each of stock solutions 1 and 2 to deionized water, then autoclaving and making up to 1 liter total volume.

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    © All Rights Reserved
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    of 1

    QF 09 Revision: 001

    Media Preparation Recipe Template Approved: 30APR20

    PAS (Page's Amoeba Saline Solution)


    Constituent of protozoa media

    Stocks per 500 ml

    (1) NaCl 12.0 g


    MgSO4.7H2O 0.40 g
    CaCl2.2H2O 0.60 g
    (2) Na2HPO4 14.20 g
    KH2PO4 13.60 g

    Medium per litre

    Stock solutions 1 & 2 5.0 ml each

    Make up to 1 litre deionised water. Autoclave at 15 psi for 15 minutes.

    Reviewed: 22nd December 2021

    CCAP (Culture Collection of Algae and Protozoa), SAMS Ltd,


    Created on: Scottish Marine Institute, Oban, Argyll, PA37 1QA, UK
    Page: 1 of 1
    05 Nov 2019 Tel: +44 (0)1631 559000 Fax: +44 (0)1631 559001
    Email: ccap@sams.ac.uk Web: www.ccap.ac.uk

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