THE CAPSULE Sheetse the correct an: |. The proper anti-cougulant used in differential leucocytes count: - a. Heparin 1%, b, Sodium EDTA. ¢c. Potassium oxalate, d. None of thé above 2. We canicollect venoui blood in hen from: a. Wing veins. b, Jugular veins. c. Saphenous vein. d. All ofthe above. In fray silt test the maximal resistance means: a., Heomolysls of all RBCS. - b. No héomolysis of RBCs. c. Some cells resist solution. d. Allcells resist solution. In Terky’s solution: a. Sodium chloride destruct RBCS,.. 4°. b, Mercuric chloride maintains WBCs. c. Mercurie chloride destruct WBCs, d, WBCS nuclei are stained. a. 5 million. b. 2-3 million. ¢. 20thousands. d. 15 million: ~ Leukemia in human is: a. Decrease leucocytes count < 20 thousand. b. Increase leucocytes count > 20 thousand. ¢. Increase neutrophils only. d. None of the above. .° % The MCHC equals: a. Hb content in single RBC. b, Average welght of Hb. b % in relation to size of RB In coagulation time test: a, Coagulation occur by intrinsic pathway. b. We can used non-heparin tube. c. Terminated by formation of fibrin. d. Allof the above. aut) col VIG ll com head glee10.Identify which is high percent leucocytes in heaithy bull: 1. 12, The proper anticoagulant used for determination of 13, In Haym’s solution: glucose: a a. Sodium chloride destruct RBCs. a. Heparin, b. Mercuric chloride destruct RBCs. b. So florid. c. Mercuric chloride destruct WBCs. ¢c. Sodium citrate. d. None of above. : d. All of the above. ‘ 14. In fragility of RBCs the minimal resistance means: a. Most RBCs heomolysis. a. Neutrophils. b. No heomolysis of RBCs. b. Esinophils. c. Some cell start to heomol . Monocytes. son . d. All cells resist heomolysis. d. Lymphocytes. : 15._By using anti A, anti D & anti B you can determine the In coagulation time test which of the following not following blood groups: id for clot formation: . : a. Group A 4ve. re a, Factor} . . : b, Group B—ve. b. Factor Il 7 c. Group AB-ve. ~~ s a c. Factor tt! d. All of the above. d. Factor IV : The most numerous blood cell in healthy Cock i: a, Erythrocytes. b. Leucocytes. a ¢, Thrombocytes. d. Lymphocytes, 42) GINA agg he: : 5 COMDLEVE THE FOLLOWING: ¥ The best anticoagulant’ In sedimentation rate Is Na citrate ee sficance of leotoal nite 3.8% while if fragility use heparin. Y Physiological significance of isotonic solution ile if fragill TAA a le to facilitate counting of RBCs. : . : ‘ f v Sate means some RBC: dilution of bl. sam; : i 'v Serum differs from plasma In the following, itlacks factors | — I V.- Vill and it contain factors Vil £ikK-x Y Fragility of RBCs increase in hemolytic.jaundice & septicemia & decrease in pernici usanemia, & more serotonin. Y It's obtained if blood sample allowed to coagulate at 37°C, ¥ Haemocytometer used in RBCs count ~ WBCs. counts erm "without addition of anticoagulant, . [0° count:but Hb measured by using Sahili's method where ‘ HCL/10 react with Hb to form-acid heainatin which is brown v The heparinized cay tube ised in determinatloi{ of PCV in color. . &a balanced cap. Tube must centrifuge at 40.000 15000. rpm for 5 minutes but the non heparinized'tube usedin - Y Incownormal MCV is 52 but.MCH'14 clottingtime. _.. Vv Bleeding time means time pass until bleeding stop, “ Westergren tubes used to measure ESR’by mm/hr, we can measure the distance in mm from piasma cleared froi RBCs in blood column were the 1" reading obtained after hr, but the 2" reading obtained after 2hr. - Y Prothrombin time means time required for coagulation to gecur, - f. Y Coagulation time means time required for fibrin formation. Y The erythrocytes in sheep about 8.1m n/mm, but MCV e . . A Vv Packed cell volume increase in dehydration & Polycythemia + about 33 we can dilute RBCs using Hayems sojution which composed of mercuric chloride - DW ~NaCL~ sodium: sulphate. but ESR increase physiologically in increase age - menses — rauleaux & menopause. (632) ANE Galan ©4 38 ee Practical sheet questions n-huuman is: (a) Decreaseb‘ofileucocytes. count fess than 20 thousand Choose the correct unswer of Ihe following questions: ae creases, ofleucocytes more thar 20 thousand (1)The proper anticoagulant used in differentia Co lnreuentonnph ry ; lencocytes count is: | (a) Heparin 1% (b) Sodium EDTA, ; or i 01 i , The MCHC.equals; (Potassium oxalate EDNone of the above " ; ea equals: ' a) Hb content in:single RBC (2) We can collect venous blood in hen from: content in:singl 4 (b) Average weight of Hb GP Wing veins (8) Jugular veins @Hb% in relation’ (8)In coagulation time tests. - Sha (3) In fragility test the maximal resistance means: ° : 2 Sard (@) Coagulaticn oscurs by intringie-pa:away (@pHemolysis of all RBCs :, No heresies Fog ees 'b) We can.used non heparin tube-’ (b) No hemolysis afRBCé. (b) We can.used on heparin tube: «at sofuti (c) Terminated-by.fo (e) Some cell wesgt solution |, . ‘ a cn SE we . All of th ; *(@ All cell resist solution ' : Z@A I of the shove (4)In Terky's solution: © ~ F . (9) The proper anticoagulaut-uised for. determination of (@) Sodium chlorite deatrust RBCs glucose in blood is: a Pe ‘a) Sodium chloride destruct t . i Pet a os (a) Heparin a ri wh. (b) Mercurie chloride maintain WBCE a ; » » MGFSasivm Dovid - ‘odium citrate 3 ” (©) Mercurie chloride destruct WBCs : on “ 2 (a) Aki of the above @ Wes nucle arg stained (10) Identify which is of high. percent’ leucocytes in eh? healthy Bull: (5) Normal thrombocytes count in bird /eumm ist 4 . a. . : 6 eat 7 . (a) Neutrophils (6) Eesinophil - o ion =3 million ¢ (©) Monocytes Ly mph 0 thousand (@) 15 million : : = @Lyaphooytes by) MB : jd MBLs a 4 7 65-(1) Calculate the asmotality of 0.85%sodinm chlor. Fhiyjotonic? WH Qanm “d is not required for cott formati ’ : blood plasma? ° (a) Factor I . (b) Factor of blood plasma / : oo OF a @©pFrctor I (@ Factor ty ic and pert (12) The most numerous blood cell in healthy Cack is: ” re shrinkage 23 ella an iological Gee of isat Q@Erytitocytes (byLeucocytes ee (©) Tiirombocytes . (d) Lymphocytes (4) ee the fare Stalement: with suitable (13) In Haym’s solution: (a) Sodium chloride destruct RBCs. (1)The serunt-is differ from plastié in lacks a. contains .. _ Q)lt is obtained, if the blood sample is allowed : a wQLak.., at 37°C, without addition of cankicanguee” (b) Mercuric chloride destruct RBCs L + ©Mercuric chloride destruct WECs j (d) None 6f the above 14) In fragility of RBCs the minimal resistance means: (@) Most RBCs bemolyss (The * heparinized capillary tubes“ are used » in détetmination of ..PEM..:, and a‘batanced capillary stubes inust be centrifuged at 19..09.°.. rpm for about ‘vom minutes, but the non-heparinized one is used in... clot eq Lute (4) The Weatergren tubes are jused to measures © BSR. bye Uefa, ye, ean measures @Some Cell start to hemolysis . . (A) All celis resist to hemolysis : c . IS) By using Aati A, Anti D and:Anti B you-ein determine the following blood groups (a) Group A + (b) Group'B + the column of galas. were the first reiding is *(@) Group A +ve up B tye is : * gbiained after .ane.he npAB—we Dalat te store (5)But the second reading is obtained, afte com hes. ago Be: 2 : raion ea Frew on <.. but: the RBCs a FH Bahay. Nabii Abu-Heakal and ¥. El Sead: Physiology 2013. Faculty of V9ts Mé University. F. El Bahay. Nabil Abu-Heakal and,.¥. El Seady. Experimental Physiology 20]3. Manso.ra University. composed of Ganong WF, Review of Medical Physiology, 2005, Mc Graw Hill-Lange: called WFG. : Nabil Abu-Heakal, Hard book of Vet. Physiology’ 2001, Ome fiagitiy’ x casé of - Faculty of Vet. Medicine, Mansoura U:ivérsity. wo Bemelyhis. 7 esndste and septielt but deceases in . and .pecaiceus anemia . Ts * to from, aig: x . which is eau rol In cow némmat MCV i s abit. 182. polycytbeania. ‘vied but BSR js ingreased physiologically i prgnangy.... MNONSES, ae Kaaleaer id, Aenopouse — 7 . : : - 69-Manioura university - - a Faoulty of veterinary medicine Date: solarons Physiology deparement ‘Viene.allowed! 16 mis. Practical sheet exam for biochemistry diploma ( postgraduate) |- Choose the correct answer :- 1- The anticoagutant act as chelating to Ca” in its mechanism of aétion is:- a Heparin 1% 2 potassium citrate _ & Sod: EDTA d- None of the above 2- Coltection of venous blond in dog from ° a> Jugular vein b- Cephalic vein & Saphenous vein d— All of the above - In Hb determination by hemometer, the concept of theory based on. a- Match blood color by stander one ‘b- Dilute blood color theti match it c- Convert color to more stable then match all the above process 4— in fragility test, minimal resistance pointmeans © = SSS *—Most RBC heamolysis ¢- All cell resist solution A~ The predominant leukocyte of normal sheep is a+ lymphocyte ‘b- neutrophil ¢- eosinophil None ofthe above I- conipletes the following statement:- 1- Capillary tube is the best method for blood collection in 2~ blood cells counted - of b- No heamolysis of RBC d- Some cell resist soliition 3 - Blood grouping in human baséd’on three 4 - Turke,s solution contain 5 - dicumarol poisoning could .... Iil- mark.true or false against each statement . of the following - 1- Reticulocyte is less resistant to hypotonic solution than RBC 22 All blood cells of birds are nucleated - _ 3:shematoctit-valite is:more indicative for anemia than blood indices ESRerate couldincrease in conditionsof hypo-proteinemia vOf heuttophil nuclease is indicative of cell ageing.