900 TS Operators Manual - 1561000 Rev C

Absorbance Microplate Reader
Operator’s Manual

800™ TS
Absorbance Reader
Operator's Manual
BioTek® Instruments, Inc.

© October 2017
PN 1561000, Rev C
ii | Notices
Notices
Highland Park, P.O. Box 998
Winooski, Vermont 05404-0998 USA
All Rights Reserved

© 2017, BioTek® Instruments, Incorporated. No part of this publication may be reproduced, transcribed, or
transmitted in any form, or by any means electronic or mechanical, including photocopying and recording, for any
purpose other than the purchaser’s use without written permission of BioTek Instruments, Inc.
Trademarks
BioTek® is a registered trademark, and 800™ TS and Gen5™ are trademarks of BioTek Instruments, Inc. BioCell™ is
a trademark of BioTek Instruments and is patented under U.S. patent number 5,963,318.
Microsoft®, Windows®, and Excel® are either registered trademarks or trademarks of Microsoft Corporation in
the United States and/or other countries.
All other trademarks are the property of their respective holders.
Restrictions and Liabilities

Information in this document is subject to change and does not represent a commitment by BioTek Instruments,
Inc. Changes made to the information in this document will be incorporated in new editions of the publication. No
responsibility is assumed by BioTek for the use or reliability of software or equipment that is not supplied by
BioTek or its affiliated dealers.
BioTek Instruments, Inc.

Contents| iii
Contents
Notices ii
Contents iii
Contact Information vi
Revision History vii
Document Conventions viii
Intended Use Statement ix
Quality Control ix
Warranty and Product Registration ix
Warnings ix
Hazards x
Precautions xi
CE Mark xiii
Electromagnetic Interference and Susceptibility xiv
User Safety xv
Safety Symbols xvi
Introduction 1
Product Description 2
Package Contents 3
Optional Accessories 3
Product Support and Service 5
Installation 7
Product Registration 8
Important Information 8
1. Unpack and Inspect the Reader 8
2. Remove the Shipping Hardware 9
3. Install the Optional Door in Top Cover 10
4. Verify the Filter Wheel Contents 11
5. Select an Appropriate Location 14
800 TS Operator's Manual

iv | Contents
6. Install the Power Supply 15

7. (Optional) Install the Printer 15
8. (Optional) Install Gen5 on the Host Computer 16
9. (Optional) Install the USB Driver 17
10. (Optional) Connect the Host Computer 17
11. Turn on the Reader 17
12. Set Date and Time on Touchscreen 18
13. Verify the Reader's Filter Table 18
14. (Optional) Establish Communication 19
15: Run a System Test 20
Operational/Performance Qualification 23
Repackaging and Shipping Instructions 24
Getting Started 27
External Components 28
Operate the Reader Using the Touchscreen 31
Operate the Reader Using Gen5 Software 38
Recommendations for Optimum Performance 41
Maintenance 43
Maintenance Overview 44
Warnings and Precautions 45
Clean Exposed Surfaces 45
Clean the Touchscreen 46
Decontamination 47
Filter Storage and Handling 49
Replacing and Aligning the Bulb 49
Instrument Qualification Process 51
Instrument System Test 52
Absorbance Testing 52
Instrument Qualification Procedures 61
Overview 62

Contents| v
IQ/OQ/PQ Description 62
Recommended Qualification Schedule 64
System Test 64
Absorbance Plate Test 66
Absorbance Liquid Tests 68
Specifications 75
General Specifications 76
Absorbance Specifications 77
Error Codes 81
Error Codes Overview 82
Error Codes 82
Sample Reports 89
Sample System Report 90
Sample Absorbance Test Plate Report 91
Sample Assay Results 92

vi | Contact Information
Contact Information
Highland Park, P.O. Box 998
Winooski, Vermont 05404-0998 USA
Global Service and Support

BioTek instrument service and repair is available worldwide at one of BioTek's
International Service Centers and in the field at your location. To arrange for service or
repair of your instrument, contact the office nearest you; visit www.biotek.com for up-to-
date contact information. For customer service, sales, and technical assistance, refer to the
information below.
Customer Service and Sales

Internet: www.biotek.com
Phone: 888-451-5171 (toll-free in the U.S.)
802-655-4740 (outside the U.S.)
Fax: 802-655-7941
Email: customercare@biotek.com
Service/Technical Assistance Center (TAC)

Phone: 800-242-4685 (toll-free in the U.S.)
802-655-4740 (outside the U.S.)
Fax: 802-654-0638
Email: tac@biotek.com
European Coordination Center/Authorized European Representative

BioTek® Instruments GmbH
Kocherwaldstrasse 34
D-74177 Bad Friedrichshall
Germany
Internet: www.biotek.de
Phone: +49 (0) 7136 9680
Fax: +49 (0) 7136 968 111
Email: info@biotek.de

Revision History| vii
Revision History
Rev Date Changes
A 5/2017 Release to production
B 8/2017 Preface and Installation chapter: Deleted the word “ambient” when describing
specified temperature ranges; corrected Fahrenheit value of the low end of the specified
temperature range.
Getting Started: Added note about the included blank USB stick.
Specifications:Added the Fahrenheit temperature values.
C 10/2017 Preface: Updated wording of Reader Data Reduction Protocol Hazard to read “All information
displayed on the screen, sent to an attached printer, or exported via computer
control...”
Introduction: Fixed a typo in the PN for the Tungsten replacement lamp kit for
800TS and 800TSI.

viii | Document Conventions
Document Conventions
This icon calls attention to important safety notes.
A Warning indicates the potential for bodily harm and tells you how to avoid the
Warning!
problem.
A Caution indicates potential damage to the instrument and tells you how to avoid the
Caution
problem.
Note Bold text is primarily used for emphasis.
Topics that apply only to specific 800 TS models are preceded by a notice in italic, for
italic
example, Applies only to 800 TS models with incubation.
This icon calls attention to important information.

Intended Use Statement| ix
Intended Use Statement

The 800 TS is a single-channel, filter-based absorbance reader designed to perform
measurements of samples in a microplate format. The performance characteristics of the
data reduction software have not been established with any laboratory diagnostic assay.
Users must evaluate this instrument and PC-based software in conjunction with their
specific assay(s). This evaluation must include the confirmation that performance
charactertistics for the specific assay(s) are met.
Quality Control
It is considered good laboratory practice to run laboratory samples according to
instructions and specific recommendations included in the assay package insert for the test
to be conducted. Failure to conduct Quality Control checks could result in erroneous test
data.
Warranty and Product Registration

Please take a moment to review the warranty information that shipped with your product.
Please also register your product with BioTek to ensure that you receive important
information updates about the product(s) you have purchased.
You can register online through the Customer Resource Center (CRC) at www.biotek.com
or by calling 888-451-5171 or 802-655-4740.
Warnings
Operate the instrument on a level, stable surface away from excessive humidity.
Bright sunlight or strong incandescent light can reduce the linear performance
range of the instrument.
Measurement values may be affected by extraneous particles (such as dust) in the
microplate wells. A clean work area is necessary to ensure accurate readings.
When operated in a safe environment according to the instructions in this
document, there are no known hazards associated with the instrument. However,
the operator should be aware of certain situations that could result in serious
injury; these may vary depending on the instrument model. See Hazards and
Precautions.

x | Hazards
Hazards
The following hazards are provided to help avoid injury:
Warning! Power Rating. The instrument’s power supply or power cord must
be connected to a power receptacle that provides voltage and current within
the specified rating for the system. Use of an incompatible power receptacle
may produce electrical shock and fire hazards.
Warning! Electrical Grounding. Never use a plug adapter to connect primary
power to the external power supply. Use of an adapter disconnects the utility
ground, creating a severe shock hazard. Always connect the power cord
directly to an appropriate receptacle with a functional ground.
Warning! Service. Only qualified technical personnel should perform service
procedures on internal components.
Warning! Accessories. Only accessories that meet the manufacturer's
specifications shall be used with the instrument.
Warning! Lubricants. Do not apply lubricants to the microplate carrier or
carrier track. Lubricant on the carrier mechanism or components in the
carrier compartment will attract dust and other particles, which may obstruct
the carrier path and cause the instrument to produce an error.
Warning! Liquids. Avoid spilling liquids on the instrument; fluid seepage into
internal components creates a potential for shock hazard. If a spill occurs
while a program is running, abort the program and turn off the instrument.
Wipe up all spills immediately. Do not operate the instrument if internal
components have been exposed to fluid. Contact BioTek TAC for assistance.
Warning! Unspecified Use. Failure to operate the equipment according to the
guidelines and safeguards specified in this manual could result in a hazardous
condition.
Warning! Software Quality Control. The operator must follow the
manufacturer’s assay package insert when modifying software parameters
and establishing reading methods. Failure to conduct quality control checks
could result in erroneous test data.
Warning! Reader Data Reduction Protocol. No limits are applied to the raw
measurement data. All information displayed on the screen, sent to an
attached printer, or exported via computer control must be thoroughly
analyzed by the operator.

Precautions| xi
Warning! Hot Surface.The tungsten lamp assembly is hot when the

instrument is turned on. Turn off the reader and allow the lamp to cool for at
least 15 minutes before attempting to replace it.
Warning! Internal Voltage. Always turn off the power switch and unplug the
power supply before cleaning the outer surface of the instrument.
Warning! Potential Biohazards. Some assays or specimens may pose a

biohazard. This hazard is noted by the symbol shown here. Adequate safety
precautions should be taken as outlined in the assay’s package insert. Always
wear safety glasses and appropriate protective equipment, such as chemical-
resistant rubber gloves and apron.
Precautions
The following precautions are provided to help avoid damage to the instrument:
Caution: Service. The instrument should be serviced by BioTek-authorized
service personnel. Only qualified technical personnel should perform service
procedures on internal components.
Caution: Spare Parts. Only approved spare parts should be used for
maintenance. The use of unapproved spare parts and accessories may result in
a loss of warranty and potentially impair instrument performance or cause
damage to the instrument.
Caution Touchscreen. Do not use sharp implements to operate the
touchscreen. Using a sharp stylus or other implement may damage the display.
Caution: Environmental Conditions. Do not expose the system to temperature
extremes. For proper operation, the temperature near the instrument should
remain within the range listed in Appendix A, Specifications. Performance may
be adversely affected if temperatures fluctuate above or below this range.
Caution: Sodium Hypochlorite. Do not expose any part of the instrument to the
recommended diluted sodium hypochlorite solution (bleach) for more than 20
minutes. Prolonged contact may damage the instrument surfaces. Be certain to
rinse and thoroughly wipe all surfaces.
Caution: Power Supply. Use only the power supply shipped with the instrument.
Operate this power supply within the range of line voltages listed on it.

xii | Precautions
Caution: Shipping Hardware.The shipping hardware must be removed before

operating the instrument and reinstalled before repackaging the instrument for
shipment.
Caution: Disposal. Dispose of the instrument according to Directive
2012/19/EU, “on waste electrical and electronic equipment (WEEE)” or local
ordinances.
Caution: Warranty. Failure to follow maintenance protocols may void the
warranty. See Chapter 4, Maintenance.
Caution: Electromagnetic Environment. Per IEC 61326-2-6 it is the user’s
responsibility to ensure that a compatible electromagnetic environment for this
instrument is provided and maintained in order that the device will perform as
intended.
Caution: Electromagnetic Compatibility. Do not use this device in close
proximity to sources of strong electromagnetic radiation (e.g., unshielded
intentional RF sources), because these may interfere with the proper operation.

CE Mark| xiii
CE Mark
Based on the testing described below and information
contained herein, this instrument bears the CE mark
Refer to the Declaration of Conformity for more specific information.
Directive 2014/30/EU: Electromagnetic Compatibility
Emissions—Class A
The system has been type-tested by an independent, accredited testing laboratory and
found to meet the requirements of EN 61326-1: Class A for Radiated Emissions and Line
Conducted Emissions.
Verification of compliance was conducted to the limits and methods of EN 55011 – (CISPR
11) Class A. In a domestic environment it may cause radio interference, in which case you
may need to mitigate the interference.
Immunity
The system has been type-tested by an independent, accredited testing laboratory and
found to meet the requirements of EN 61326-1 and EN 61326-2-6 for Immunity.
Verification of compliance was conducted to the limits and methods of the following:
EN 61000-4-2, Electrostatic Discharge
EN 61000-4-3, Radiated EM Fields
EN 61000-4-4, Electrical Fast Transient/Burst
EN 61000-4-5, Surge Immunity
EN 61000-4-6, Conducted Disturbances from RFI
EN 61000-4-11, Voltage Dips, Short Interruptions and Variations
Directive 2014/35/EU Low Voltage (Safety)

The system has been type-tested by an independent testing laboratory and was found to
meet the requirements of this Directive. Verification of compliance was conducted to the
limits and methods of the following:
EN 61010-1. "Safety requirement for electrical equipment for measurement, control and
laboratory use. Part 1, General requirements."
EN 61010-2-081. “Particular requirements for automatic and semi-automatic laboratory
equipment for analysis and other purposes.”
EN 61010-2-010. “Particular requirements for laboratory equipment for the heating of
materials.“

xiv | Electromagnetic Interference and Susceptibility
Directive 2012/19/EU: Waste Electrical and Electronic Equipment

Disposal Notice: Dispose of the instrument according to Directive 2012/19/EU, “on waste
electrical and electronic equipment (WEEE)” or local ordinances.
Directive 98/79/EC: In Vitro Diagnostics (if labeled for this use)

l Product registration with competent authorities
l EN 61010-2-101. “Particular requirements for in vitro diagnostic (IVD) medical
equipment.”
l Traceability to the U.S. National Institute of Standards and Technology (NIST).
Electromagnetic Interference and Susceptibility
USA FCC CLASS A
RADIO AND TELEVISION INTERFERENCE
NOTE: This equipment has been tested and found to comply with the limits for a Class
A digital device, pursuant to Part 15 of the FCC Rules. These limits are designed to provide
reasonable protection against harmful interference when the equipment is operated in a
commercial environment. This equipment generates, uses, and can radiate radio frequency
energy and, if not installed and used in accordance with the instruction manual, may cause
harmful interference to radio communications. Operation of this equipment in a
residential area is likely to cause harmful interference, in which case the user will be
required to correct the interference at their own expense.
In order to maintain compliance with FCC regulations, shielded cables must be used with
this equipment. Operation with non-approved equipment or unshielded cables is likely to
result in interference to radio and television reception.
Canadian Department of Communications Class A

This digital apparatus does not exceed Class A limits for radio emissions from digital
apparatus set out in the Radio Interference Regulations of the Canadians Department of
Communications.
Le present appareil numerique n'emet pas du bruits radioelectriques depassant les limites
applicables aux appareils numerique de la Class A prescrites dans le Reglement sur le
brouillage radioelectrique edicte par le ministere des Communications du Canada.

User Safety| xv
User Safety
This device has been type-tested by an independent laboratory and found to meet the
requirements of the following:
l Underwriters Laboratories UL 61010-1, “Safety requirements for electrical equipment
for measurement, control and laboratory use; Part 1: General requirements.”
l Canadian Standards Association CAN/CSA C22.2 No. 61010-1, “Safety requirements
for electrical equipment for measurement, control and laboratory use; Part 1:
General requirements.”
l EN 61010 Standards, see CE Mark starting on page xiii.

xvi | Safety Symbols
Safety Symbols
Some of the following symbols may appear on the instrument or accessories:
Alternating current Warning, risk of crushing or pinching
Courant alternatif Attention, risque d'écrasement et pincement
Wechselstrom Warnen, Gefahr des Zerquetschens und
Corriente alterna Klemmen
Corrente alternata Precaución, riesgo del machacamiento y
sejeción
Attenzione, rischio di schiacciare ed
intrappolarsi
Direct current Warning, hot surface
Courant continu Attention, surface chaude
Gleichstrom Vorsicht, heiße Oberfläche
Corriente continua Precaución, superficie caliente
Corrente continua Attenzione, superfice calda
Both direct and alternating Laser radiation: Do not stare into beam
current Rayonnement laser: Ne pas regarder dans le
Courant continu et courant faisceau
alternatif Laserstrahlung: nicht in den strahl blicken
Gleich - und Wechselstrom Radiación de laser: No mire fijamente al rayo
Corriente continua y Radiazione di laser: Non stare nel fascio
corriente alterna
Corrente continua e
corrente alternata
Earth ground terminal Warning, potential biohazards
Borne de terre Attention, risques biologiques potentiels
Erde (Betriebserde) Warnung! Moegliche biologische Giftsoffe
Borne de tierra Atención, riesgos biológicos
Terra (di funzionamento) Attenziones, rischio biologico
Protective conductor Caution (refer to accompanying documents)
terminal Attention (voir documents
Borne de terre de d'accompanement)
protection Achtung siehe Begleitpapiere
Schultzleiteranschluss Atención (vease los documentos incluidos)
Borne de tierra de Attenzione, consultare la doc annessa
protección
Terra di protezione

Safety Symbols| xvii
On (Supply) Consult instructions for use

Marche (alimentation) Consulter la notice d'emploi
Ein (Verbindung mit dem Gebrauchsanweisung beachten
Netz) Consultar las instrucciones de uso
Conectado Consultare le istruzioni per uso
Chiuso
Off (Supply) In vitro diagnostic medical device
Arrêt (alimentation) Dispositif médical de diagnostic in vitro
Aus (Trennung vom Netz) Medizinisches In-Vitro Diagnostikum
Desconectado Dispositivo médico de diagnóstico in vitro
Aperto (sconnessione dalla Dispositivo medico diagnostico in vitro
rete di alimentazione)
Warning, risk of electric Separate collection for electrical and
shock electronic equipment
Attention, risque de choc Les équipements électriques et électroniques
électrique font l'objet d'une collecte sélective
Gefährliche elektrische Getrennte Sammlung von Elektro- und
schlag Elektronikgeräten
Precaución, riesgo de Recogida selectiva de aparatos eléctricos y
sacudida eléctrica electrónicos
Attenzione, rischio di scossa Raccolta separata delle apparecchiature
elettrica elettriche ed elettroniche

xviii | Safety Symbols

Chapter 1
Introduction
This chapter introduces the 800 TS and provides contact information for
technical assistance.
Product Description 2
Package Contents 3
Optional Accessories 3
Product Support and Service 5
2 | Chapter 1: Introduction
Product Description
The 800 TS is a compact, filter-based, single-channel absorbance microplate reader. All
models are equipped with a touchscreen interface and support endpoint dual-wavelength
reads from 400-750 nm on standard 6- to 96-well plates. The narrow beam (NB) model
supports 384-well plates. UV models support a measurement range from 340-750 nm.
Some models offer incubation to 50˚C and/or linear plate shaking.
Basic data analysis, reporting, and exporting are provided via the touchscreen. With
optional Gen5 software, kinetic and well area scanning read modes are supported, along
with BioCell and 60-, 72-, and 96-well Terasaki plates. Gen5 also offers extensive data
analysis and reporting and exporting capabilities. The reader is available in five models.
Model Measurement Range Default Filters (in nm) Plate Types Shaking Incubation
800TS 400-750 nm 405, 450, 490, 630 6- to 96-well yes no
800TSI 400-750 nm 405, 450, 490, 630 6- to 96-well yes yes
800TSNB 400-750 nm 405, 450, 490, 630 6- to 384-well no no
800TSUV 340-750 nm 340, 405, 450, 490, 630 6- to 96-well yes no
800TSUVI 340-750 nm 340, 405, 450, 490, 630 6- to 96-well yes yes
The filter wheel contains up to five filters and is user accessible. For models with fewer
than five filters, removable plugs are installed in the empty wheel locations.
The 800TS and 800TSI models use a tungsten bulb as their light source. The other models,
800TSUV, 800TSUVI, and 800TSNB, use a halogen bulb.
Use of labware other than those described here can result in positioning errors during program
execution.
Models with incubation capability are equipped with a door. This door is also available
for purchase as an accessory for non-incubation-capable models. If you order a door
separately, you must install it yourself.
See Appendix A, Specifications, for performance and technical specifications.

Package Contents | 3
Package Contents
Package contents and part numbers are subject to change. Please contact BioTek Customer
Care with any questions.
Item Part #
800 TS Operator's Manual on USB flash drive 1561000
Non-incubation models: 01281
Power supply
Incubation models: 02395
Power cord varies according to country of use
USB cable 75108
Blank 4 GB USB flash drive 01087
Dust cover 7332040
Gen5 RC software GEN5RC
Optional Accessories
Accessory availability and part numbers are subject to change. Please contact BioTek
Customer Care if you have any questions or visit www.biotek.com and use the
Accessories search tool.
Item Part #
7-filter Absorbance Test Plate for absorbance

7260522
measurement testing
Absorbance Test Plate for absorbance
7260551
measurement testing at 340 nm
800 TS Product Qualification (IQ-OQ-PQ)
1560516
package
Accessory door for top cover 1560013
RP-D10 Seiko thermal printer 02434
Power cord for printer varies according to country of use
BioCell quartz vessel 7272051

Item Part #
BioCell adapter plate (holds up to 8 BioCells) 7270512

Terasaki adapter plate 7330531
Cuvette holder 7302030
Tungsten replacement lamp kit for 800TS and
1560532
800TSI
Halogen replacement lamp kit for 800TSUV,
1560533
800TSUVI, and 800TSNB
7334 + wavelength (e.g., 7334405,
Filters
7334540)
Visit www.biotek.com or contact your
Gen5 software/upgrade
local dealer for details
Materials for Liquid Tests Part #

BioTek Wetting Agent Solution (PN 7773002) 7773002
BioTek QC Check Solution No. 1 (25 mL) or 7120779
BioTek QC Check Solution No. 1 (125 mL) 7120782
β-NADH Powder (β-Nicotinamide Adenine Dinucleotide, Sigma #N6785-10VL (or BioTek
Reduced Form) PN 98233)
Phosphate-Buffered Saline (PBS) Tablets (pH 7.2–7.6) Sigma #P4417

Product Support and Service | 5
Product Support and Service
See page vi for contact information for BioTek Global Service and Support.
Technical Assistance Center (TAC)

If your instrument or software fail to function properly, if you have questions about how to
use or maintain our products, or if you need to send an instrument to BioTek for service or
repair, please contact our Technical Assistance Center (“TAC”).
TAC is open from 8:30 AM to 5:30 PM (EST), Monday through Friday, excluding standard
U.S. holidays.
l Phone: (800) 242-4685 or (802) 655-4740
l Fax: (802) 654-0638
l E-Mail: tac@biotek.com
l Web: www.biotek.com
Please be prepared to provide the following information:
l Your name and company information, along with a daytime phone or fax number,
and/or an e-mail address
l The product name, model, and serial number (the serial number is located on the
right side of the reader)
l The onboard software part number and basecode version
l Via the touchscreen by tapping Instrument on the Main Menu.
l Via Gen5 for the 800 TS by selecting System > Instrument Configuration,
select 800 TS, then click View/Modify > Setup, select the Basecode tab, and
click Get Basecode Information
l For troubleshooting assistance or instruments needing repair, the specific steps that
produce your problem and any error codes displayed on the touchscreen or in Gen5
(see also Appendix B, Error Codes)
l Gen5 users: A text file of the diagnostic history of the instrument (select System
> Diagnostics > History, then select the appropriate file and click Export)

Running a system test when a problem occurs provides valuable information for TAC.
When the test is complete, save it from the touchscreen to a USB flash drive or, in
Gen5, click Save As to save a text file of the system test report, which can be emailed
to TAC.
If you need to return an instrument to BioTek for service or repair, please contact the TAC
for a Service Call Notice (SCN) number and the shipping address. Repackage the
instrument according to the instructions at the end of Chapter 2, Installation.

Chapter 2
Installation
This chapter includes instructions for unpacking and setting up the 800 TS.
Instructions are also included for preparing the reader for shipment.
Product Registration 8
Important Information 8
1. Unpack and Inspect the Reader 8
2. Remove the Shipping Hardware 9
3. Install the Optional Door in Top Cover 10
4. Verify the Filter Wheel Contents 11
5. Select an Appropriate Location 14
6. Install the Power Supply 15
7. (Optional) Install the Printer 15
8. (Optional) Install Gen5 on the Host Computer 16
9. (Optional) Install the USB Driver 17
10. (Optional) Connect the Host Computer 17
11. Turn on the Reader 17
12. Set Date and Time on Touchscreen 18
13. Verify the Reader's Filter Table 18
14. (Optional) Establish Communication 19
15: Run a System Test 20
Operational/Performance Qualification 23
Repackaging and Shipping Instructions 24
8 | Chapter 2: Installation
Product Registration
Please register your product(s) with BioTek to ensure that you receive important
information and updates about the product(s) you have purchased.
Register online through BioTek’s Customer Resource Center (CRC) at www.biotek.com or
by contacting BioTek Customer Care at (888) 451-5171 or (802) 655-4740.
Important Information
This chapter contains installation tasks for the 800 TS and accessories. Perform
the tasks in the order presented, skipping those that do not apply to your reader’s
configuration.
Remove the shipping hardware before turning on the instrument.
Reinstall the shipping hardware before repackaging the instrument for shipment.
1. Unpack and Inspect the Reader

Save all packaging materials. If you need to ship the reader to BioTek for repair or
replacement, you must use the BioTek-supplied materials. Using other forms of
commercially available packaging, or failing to follow the repackaging instructions,
may void your warranty.
During the unpacking process, inspect the packaging, reader, and accessories for
shipping damage. If the reader is damaged, notify the carrier and your BioTek
representative. Keep the shipping boxes and the packaging materials for the carrier's
inspection. BioTek will arrange for repair or replacement immediately.
1. Open the shipping box, remove the accessories tray, and then remove the instrument
from the box and place it on a level, stable surface.
2. Place the packaging materials back into the shipping box for reuse if the instrument
needs to be shipped again.

2. Remove the Shipping Hardware | 9
2. Remove the Shipping Hardware
Remove and store all shipping hardware before you turn on the reader.
1. Carefully turn the reader upside down on a level surface.

2. Remove the six black screws holding the cover to the base, then lift the base off the
cover and set it right side up on a level surface.
3. Remove the two screws on each of the shipping brackets, remove the brackets, and
store the screws in the holes on the brackets.

4. Store the shipping hardware in a safe place in case it is needed in the future.
5. If applicable (described in the next section), install the door now.
3. Install the Optional Door in Top Cover

Applies to models for which the door was ordered as a separate accessory.
1. If you have not already done so, remove the top cover by carefully turning over the
reader and removing the six screws that attach the top cover to the base.
2. Remove the two hole plugs from the top cover. You do not need to retain these plugs.
3. Using the nuts and washers that ship with the door, attach the door to the top cover as
shown next.

4. Verify the Filter Wheel Contents | 11
If you have installed the door on an existing reader (i.e., outside of the instrument
installation process), you can reattach the top cover now. Otherwise, leave the top
cover off and continue to 4. Verify the Filter Wheel Contents.
4. Tape the door closed, then carefully turn the reader upside down over its cover, and
reattach the cover to the reader by screwing in the six screws.
4. Verify the Filter Wheel Contents

The 800 TS ships with up to five preordered filters in the filter wheel. During installation, it
is good practice to verify the filters and their placement, and to confirm that the software
filter table matches the filter wheel’s configuration (discussed later). All five locations in
the wheel must contain either a filter or a plug.
Before continuing, obtain a clean, lint-free cloth.

For models with incubation capability: You must first remove the incubation housing to
access the filter wheel.
1. With the top case removed from the reader (see Remove the Shipping Hardware
on page 9 for directions), remove the four thumbscrews from the optic arm, and lift
the optic arm cover off (if equipped).
2. Disconnect the two connectors on the back of the incubation housing, then gently lift
the housing off of the reader.

4. Verify the Filter Wheel Contents | 13
To access the filter wheel:
1. With the top case removed from the reader

(see Remove the Shipping Hardware on page 9
for directions), remove the four thumbscrews
from the filter wheel cover. This cover is directly
under the optics arm.
Note: The filters are not held in place and fall out of the wheel easily, so make sure to have a clean, lint-
free cloth in place before turning over the filter wheel.
2. Lift the filter wheel off its pin, then remove
the filters by turning the wheel upside down
over a clean, lint-free cloth. Write down which
filters are installed in the filter wheel and the
location of each filter. You will use this
information later to verify/edit the software
filter table.
Compare the filters with your model’s default
filters (see the table on page 2) or with the
purchase order if different filters were ordered.
Contact BioTek Customer Care if you did not
receive the expected filters/plugs.
Each filter is labeled with a wavelength and an

arrow showing the light direction. The arrows
must point downward when the filter wheel is
installed on the reader.

3. When you are finished examining the filters,

replace the filters in the filter wheel, being
careful to insert them in the correct direction
(indicated by the arrow printed on the side of
each filter).
4. Replace the filter wheel on its pin, and then
replace the filter wheel cover using the four
thumbscrews removed during step 1.
5. Tape the door closed, if equipped.
6. Carefully turn the reader upside down over its
cover, and reattach the reader to the top cover
by replacing the six screws.
5. Select an Appropriate Location

Install the reader on a level, stable surface. Select an area where temperatures between
18°C (64°F) and 40°C (104°F) can be maintained.
The reader is sensitive to extreme environmental conditions. Avoid the following:
l Excessive humidity. Condensation directly on the sensitive electronic circuits can
cause the instrument to fail internal self-checks. The humidity must be in the range of
10–85%, non-condensing.
l Excessive ambient light. Bright light may affect the reader’s optics and readings,
reducing its linear range.
l Dust. Readings may be affected by extraneous particles (such as dust) in the
microplate wells. A clean work area is necessary to ensure accurate readings.

6. Install the Power Supply | 15
6. Install the Power Supply

Power Rating. The instrument must be connected to a power receptacle that
provides voltage and current within the specified rating for the system. Use of an
incompatible power receptacle may produce electrical shock and fire hazards.
Electrical Grounding. Never use a plug adapter to connect primary power to the
instrument. Use of an adapter disconnects the utility ground, creating a severe
shock hazard. Always connect the system power cord directly to an appropriate
receptacle with a functional ground.
1. Connect the power cord to the external power supply.

2. Locate the power inlet on the rear of the reader.
3. Plug the rounded end of the power supply's cord into the power inlet.
4. Plug the other end of the power cord into an appropriate power receptacle.
7. (Optional) Install the Printer
Unpack the Printer
1. Open the printer’s shipping box, and remove the top foam tray.
2. Carefully remove the printer and its components from the shipping box.
Note that the printer is shipped with two adapter plates for use with 58 mm paper.
Unless you plan to use that size of paper roll, you do not need to use these adapter
plates.

Install the Printer
1. Insert the round end of the power supply cord into the DC power port on the rear of the
printer, then attach one end of the power cord to the power supply box and plug the
other end into a power outlet.
2. Insert one end of the supplied USB cable into the USB printer port on the rear of the
instrument and the other end in the USB port on the printer.
Do not use the USB port on the front of the 800 TS; that port is for USB flash drives
only.
8. (Optional) Install Gen5 on the Host Computer

There is a certain sequence of events that must be followed to ensure that the
software is properly installed and configured. Please follow the instructions
provided in Gen5 Getting Started Guide to install the software.

9. (Optional) Install the USB Driver | 17
9. (Optional) Install the USB Driver
Refer to the instructions that shipped with the Gen5 software to install the necessary
drivers. The driver must be installed on the computer before you connect the
instrument.
10. (Optional) Connect the Host Computer
The USB port is located on the rear of the reader.
1. Turn off the computer. If the reader is on, turn it off.

2. Using the supplied USB cable, connect the square end of the cable to the USB port on
the back of the reader.
3. Connect the other end of the cable to an available USB port on the computer.
11. Turn on the Reader
1. Locate the power on/off switch on the right side of the instrument, and turn on the
reader. The reader delays any action until the bulb has warmed up: three minutes for
narrow beam and UV models, and 30 seconds otherwise. Then the reader performs a
power-up system test.
If using Gen5, do not attempt to communicate with the reader until the system
test is finished.
2. When the system test is completed, the touchscreen displays its main screen.
If an error occurs during the system test, the reader beeps and the error code is displayed
on the touchscreen. Make note of the error code number, then tap OK to stop the beeping.
See Appendix B, Error Codes, for information about the error code you noted.

12. Set Date and Time on Touchscreen

The date and time are included in the instrument’s system test report. Define these
settings now to ensure correct information when you perform step 15.
1. From the Main Menu, tap Instrument, the Config tab,
then the Time button.
2. Tap the hour value, and use the keypad to enter the
correct time for both the hour and minutes, then click OK.
Note: You can change just the minutes value: Tap the
minutes value and enter the correct time.
3. Tap the Date button.
4. Tap month, day, or year, and use the keypad to set the
current date. Tap OK when done.
13. Verify the Reader's Filter Table

Before using the 800 TS, verify that the filter table reflects the filters installed in the filter
wheel.
Important! The reader’s filter table must exactly match the contents of the installed
filter wheel.

14. (Optional) Establish Communication | 19

and then Filter Table. Filter wheel locations 1 through 5
are shown, with each location’s filter value (in nm) or
“plug” for a blank filter.
2. Verify that the values match the contents of the filter
wheel. See the Installation chapter for instructions for
accessing the filter wheel.
3. To change the setting for a filter wheel position, tap its
value and use the keypad to enter a wavelength value (in
nm), or select Plug. Tap OK when finished.
4. If you made any changes, tap Save in the Installed Filters
screen. The filter values are now available for selection in
protocols and the absorbance test plate setup screen.
See the values you wrote down in step 2 of "4. Verify the Filter Wheel Contents" on
page 11. If there is a mismatch, correct the Filter Table to match the filters in the filter
wheel. See "Verify or Change the Reader’s Filter Table" on page 33 for instructions.
14. (Optional) Establish Communication
NOTE: Instrument must be at the Main Menu screen for Gen5 communication to occur.
1. On the host computer, start Gen5 and log in if prompted. The default System
Administrator password is admin.
2. From the Gen5 main screen, select System > Instrument Configuration and click Add.
3. Set the Reader Type to 800 TS.
4. Perform one of the following steps, as applicable:
l Select Plug & Play.
An 800 TS must be connected via USB to the computer and turned on to appear
in the Available Plug & Play Readers list.
l Set the Com Port to the computer's COM port to which the reader is connected.

The information can be found via the Windows Control Panel, under Ports in the
Hardware/Device Manager area of System Properties (e.g., Serial Port (COM5)).
5. To verify that Gen5 can communicate with the instrument, click Test Comm. If the
communication attempt is successful, Gen5 displays a success message. Return to
Gen5’s main screen.
Communication Errors
If the communication attempt is not successful, try the following:
l Is the reader connected to the power supply and turned on?
l Is the communication cable firmly attached to both the reader and the computer?
l Did you select the correct Reader Type in Gen5?
l Try a different COM Port in Gen5 or use Plug & Play.
l Did you install the USB driver software?
l Is the touchscreen at the Main Menu?
If you remain unable to get Gen5 and the reader to communicate with each other,
contact BioTek’s Technical Assistance Center.
15: Run a System Test

Running a system test will confirm that the reader is functioning properly, or will provide
an error code if a problem is detected.
Instructions for performing the test using Gen5 are provided on page 22.
Using the Touchscreen
1. If the reader is equipped with an incubator, turn it on.

a. On the Main Menu, tap the temperature display (circled in the following image).
When the incubator is turned on, the dashes are replaced by the reader’s internal
temperature, in degrees Celsius.

15: Run a System Test | 21
b. On the Incubate tab of the Quick Menu screen, turn on the Temperature Control and
enter a setpoint of at least 37°C, then tap Home to return to the Main Menu.
c. Wait for the temperature display to reach the defined setpoint before continuing.
2. From the Main Menu, tap Instrument > Options.

3. Under System test, tap Start.

4. When the test finishes, tap USB Report to save the test results to a USB flash drive,
Print to print the test results, or Exit to close the screen.
5. If applicable, turn off incubator.
Using Gen5
1. If necessary, launch Gen5 and turn on the incubator:

l From the Gen5 main screen, select System > Instrument Control > 800 TS.
l Click the Pre-Heating tab.
l Enter a Requested temperature of at least 37°C and click On.
l Wait until the incubator temperature reaches the set point before continuing.
2. Return to Gen5’s main view and select System > Diagnostics > Run System Test. If
prompted to select a reader, select 800 TS and click OK.
3. If a message appears, stating that the reader has a pending system test report, click OK,
then click Close.
The reader ran a "power-up" system test, but that test did not include verifying
that the incubator reaches a set temperature. Therefore, you will run another
system test.
Again, select System > Diagnostics > Run System Test. If prompted to select a reader, select 800
TS and click OK.
4. When the test is completed, a dialog requesting additional information appears. Enter
the information and click OK.
5. The results report appears and should contain the text "SYSTEM TEST PASS."
l If required, print the report and store it with your records.
l The Gen5 software stores system test information in its database; you can retrieve
it at any time.
l You can save the system test report as a text file: click Save As in the System Test
Results dialog.

Operational/Performance Qualification | 23
If an error code is returned, refer to Appendix B and look up the code. If the
problem is something you can fix, do so now and run another system test. If the
problem is something you cannot fix, or if the test continues to fail, contact
BioTek’s Technical Assistance Center at (800) 242-4685 or (802) 655-4740.
6. If applicable, turn off the incubator:

l Select System > Instrument Control > 800 TS.
l Click the Pre-Heating tab and click Off.
l Return to Gen5’s main view.
Operational/Performance Qualification
Your 800 TS was fully tested at BioTek prior to shipment and should operate properly
following the successful completion of the installation and setup procedures described in
this chapter.
If you suspect that problems occurred during shipment, if you received the reader back
from BioTek following service or repair, or if regulatory requirements dictate that
Operational/Performance Qualification is necessary, turn to Chapter 5, Instrument
Qualification now to learn about BioTek’s recommended OQ/PQ procedures for 800 TS.
A Product Qualification & Maintenance (IQ/OQ/PQ) package for the 800 TS is available
for purchase (PN 1560516). Contact your local BioTek dealer for more information.

Repackaging and Shipping Instructions

Important! Please read all of the information provided below before preparing the 800 TS for
shipment.
Contact BioTek’s Technical Assistance Center for a service call notice (SCN)
number and the shipping address before returning equipment for service.
If the reader has been exposed to potentially hazardous material, decontaminate
it to minimize the risk to all who come in contact with the reader during shipping,
handling, and servicing. Decontamination prior to shipping is required by the U.S
Department of Transportation regulations. See the As-Needed Maintenance
chapter for decontamination instructions.
Remove any labware from the carrier before shipment. Spilled fluids can
contaminate the optics and damage the instrument.
The instrument’s packaging design is subject to change. If the instructions in this

section do not appear to apply to the packaging materials you are using, please
contact BioTek’s Technical Assistance Center for guidance.
Replace the shipping hardware before repackaging the instrument. Please

contact BioTek if you need to replace any of these items:
l Carrier shipping bracket (PN 1560517)
l Carrier shipping bracket screws (PN 19668)
l Shaft shipping bracket (PN 7332041)
l Shaft shipping bracket screws (PN 19337)
l If applicable, top incubation shipping bracket (PN 1562072)
If you need to ship the 800 TS to BioTek for service or repair, be sure to use the
BioTek-supplied packaging materials. Other forms of commercially available
packaging are not recommended and can void the warranty.
If the packaging materials have been damaged or lost, or if the same set has
been used more than four times, contact BioTek to order replacement part
number 1563000. The shipping box, accessories box, and foam trays are included
as a whole set under this part number and cannot be ordered separately.

Repackaging and Shipping Instructions | 25
Attach the Shipping Hardware
The shipping brackets must be reattached before the 800 TS can be shipped.
1. Turn off the reader, and unplug the power supply from the power outlet and from the
power supply port on the back of the reader.
2. Disconnect the USB cable(s) from the reader (if using a computer and/or printer).
3. Carefully turn the reader upside down on a level surface.
4. Remove the six black screws holding the top cover to the base, then lift the base off of
the cover and set it right side up on a level surface.
5. Place the carrier shipping bracket around the carrier rail first, then rotate the bracket
down so that it sits on the base pan.
6. Hold the carrier shipping bracket snug against the front of the carrier, then screw in the
two screws to secure it.
7. Place the shaft shipping bracket over the shaft, and, for non-incubation-capable readers,
secure it with the two screws.
8. For incubation-capable readers, place the top incubation shipping bracket over the shaft
shipping bracket, and secure the brackets with two screws.

Repackage the Instrument

Ensure that the shipping hardware has been attached as instructed in the previous section.
Refer to the next two figures when performing the following steps:
1. Place the bottom foam tray into the bottom of the shipping container.
2. If the reader has a door, tape it shut, then place the reader inside the original plastic
bag, and carefully lower the reader into the foam tray in the bottom of the box.
3. Place the accessory tray in the box, and then place the accessories into the tray as
shown in the figure.
4. Close the top of the box, and secure it with shipping tape.
5. Write the service call notice (SCN) number and shipping address in large, clear letters
on the outside of the shipping container, and ship the box to BioTek.

Chapter 3
Getting Started
This chapter provides an introduction to using the touchscreen and optional

Gen5 software to control the 800 TS. It also contains recommendations for
optimum performance.
External Components 28
Operate the Reader Using the Touchscreen 31
Operate the Reader Using Gen5 Software 38
Recommendations for Optimum Performance 41
28 | Chapter 3: Getting Started
External Components
1 Microplate carrier
2 Touchscreen
3 Power switch
4 USB flash drive port for USB flash drive
Note that the photo does not show the door.

External Components | 29
Rear ports on a reader without incubation capability
1 Power port
2 USB port
3 DO NOT USE! For BioTek Service only
4 USB port for printer
Rear ports on a reader with incubation capability
1 Power port
2 USB port
3 DO NOT USE! For BioTek Service only
4 USB port for printer

Optional Printer
Refer to the manual on CD that ships in the printer’s box for more details.
The 800 TS supports outputting the System Test report, the Absorbance Test Plate report,
and measurement results including Raw OD, Delta OD, and blanked data to a thermal
paper printer.
Load the Printer Paper
1. Ensure the printer is turned on.

2. Press the gray tab on the right side of the printer to open the door.
3. Place a roll of paper into the indented space on the back of the door with the leading
edge of the paper coming from the top of the roll.
The printer prints on only one side of the paper. Ensure that the paper roll is installed
so that the shiny side of the paper faces up. The printer will not print on the matte side
of the paper.
4. Unroll the paper just enough so that it clears the end of the door, then firmly close the
door until it clicks into place. The printer advances the paper and cuts it.
Use the Printer
l To test that the printer is properly installed and communicating with the reader, in
the Main Menu, tap Instrument, then on the Option tab, tap Start to begin the
Printer Test. A short test result prints. If the results did not print, check that the power
cable and USB cable are securely connected and run the test again.
l To print the results of a read (both a Quick Read and a defined protocol read), tap
Output in the top-right corner of the results screen displayed when the read finishes.
l To print System Test results, tap Print in the screen that appears when the system
test finishes.
l To cleanly tear the paper from the printer, pull upward from left to right.
l To advance the paper, press Feed on the front of the printer.
l To turn off the printer, press and hold the ON/OFF button for at least three seconds.

Operate the Reader Using the Touchscreen | 31
Operate the Reader Using the Touchscreen
General Information
Do not use a sharp stylus or pencil on the touchscreen. Doing so can damage the
touchscreen's surface. You can use a stylus designed for capacitive touchscreens.
When you turn on the 800 TS, the touchscreen turns on automatically and opens to the
Main Menu after the start-up system test and bulb warm-up. The length of the bulb warm-
up process varies from three minutes for narrow beam and UV models, and 30 seconds
otherwise. See Turn on the Reader in Chapter 2.
l To select a button or check box or to activate a tab, tap the item once.
l To return to the Main Menu from any other screen, tap Home in the top-left corner.
l For instructions on cleaning the touchscreen, see page 46.
l To preserve the life of the bulb, turn it off when not needed by tapping in the
Instrument or Quick Menu. Note that if you turn off the bulb, the reader will go
through the bulb warm-up process when you turn the bulb back on (three minutes for
narrow beam and UV models, and 30 seconds otherwise).
Main Menu

800TSI and 800TSUVI models: When the incubator is turned on, the reader’s internal
temperature is displayed at the top of the screen, as shown above. When the incubator
is off, dashes are displayed.
The left side of the Main Menu screen lists the assay protocols defined on the reader. As
shipped, the reader contains no protocols and the list is empty. Up to 40 uniquely named
protocols can be programmed and stored. Tap Next to scroll through the list.
On the right side of the Main Menu are the following options:
l Quick: Define and run a simple single- or dual-wavelength protocol. You can select
the primary and secondary wavelength values and the plate type. If applicable to your
reader model, shake and/or incubate options are available.
l Protocol: Edit, create (and save), delete, and copy protocols. You can define the
protocol name; select the primary and secondary wavelength values, read speed, and
plate type; and define blank well(s). If applicable to your reader mode, shake options
are available.
The reader automatically performs delta OD and blank subtraction. To select the
dataset(s) for print or export, tap Instrument and then the Output tab.
l Results: View and optionally print/export measurement data stored on the reader
(for the 12 most recently run protocols).
l Instrument: Configure the reader, printer, and USB flash drive settings; define results
output criteria; run an Absorbance Plate Test; and more.
Configure Your 800 TS

After you install the reader, and before you use it to create and run protocols, perform the
tasks in this section to define important instrument settings.
Set the Time and Date

When you turn on the 800 TS for the first time, set or confirm the date and time at your
location.


then the Time button.
2. Tap the hour value, and use the keypad to enter the
correct time for both the hour and minutes, then click OK.
Note: You can change just the minutes value: Tap the
minutes value and enter the correct time.
3. Tap the Date button.
4. Tap month, day, or year, and use the keypad to set the
current date. Tap OK when done.
Verify or Change the Reader’s Filter Table
filter wheel.


and then Filter Table. Filter wheel locations 1 through 5
are shown, with each location’s filter value (in nm) or
“plug” for a blank filter.
2. Verify that the values match the contents of the filter
wheel. See the Installation chapter for instructions for
accessing the filter wheel.
3. To change the setting for a filter wheel position, tap its
value and use the keypad to enter a wavelength value (in
nm), or select Plug. Tap OK when finished.
4. If you made any changes, tap Save in the Installed Filters
screen. The filter values are now available for selection in
protocols and the absorbance test plate setup screen.
Define Regional Settings

and then Regional Settings.
2. Tap to toggle between:
-Time format: AM/PM or 24 hour
-Date format: mm/dd/yyyy or dd/mm/yyyy
-Decimal symbol: period or comma (Note: This setting

does not apply to the system test report content.)
-List separator: comma or semicolon (used in the

exported report .csv file)
Define Output Formats for Measurement Data

If you want to send results to a printer connected to the
reader:
1. From the Main Menu, tap Instrument, then tap the

Output tab.
2. Toggle the Printer button to Enabled, and select the

data to be included on the printout (you can select more
than one):

l Raw OD: The raw measurement value for each well.

l Delta OD: Applicable only when a secondary wavelength is selected. This is the calculated
value for each well of the primary wavelength measurement minus secondary wavelength
measurement.
l Blanked. The calculated value for each well, either Raw OD minus the average of the blank
wells for single-wavelength reads or Delta OD minus the average of the blank wells for dual
wavelength reads.
If you want to send results to a USB flash drive inserted in the reader, on the Output tab,
toggle the USB Flash Drive button among the options.
l Report: Generates a CSV file containing the measurement values (with Raw OD/Delta
OD/blanked values, as applicable). This file can be opened in Excel or other spreadsheet
software.
l Gen5 Input: Generates a text file that can be opened in Gen5 using the Read from File option.
This file contains only raw data, not delta ODs or blanks.
See page 92 for sample CSV and text files.
Define and Start a Quick Protocol

1. From the Main Menu, tap Quick.
2. In the Quick Menu, set the Primary and, if

applicable, Secondary wavelengths and plate type,
then tap Start.
Note: You cannot define blank wells for a Quick read.
When the read is finished, the results are displayed.

Tap Output to send results to the printer or USB flash
Note: This screenshot is from an instrument drive.
with incubation and shake capabilities.
Create and Save a Protocol
The maximum number of uniquely named protocols that can be saved on the
touchscreen at the same time is 40.
Create a Protocol
1. From the home screen, tap Protocol, then tap Create.

2. Use the onscreen keyboard to enter a name for the

protocol, then tap OK.
Note: The <-- key circled in the figure is the backspace

key.
3. Toggle through the values to set a Primary

wavelength.
4. If applicable, toggle through the values to set a

Secondary wavelength. Otherwise, leave as “---.”
Note: The reader automatically performs Delta OD
subtraction.
5. Toggle to set a read speed: Normal, Rapid, or

Sweep. See the Throughput values associated with
Note that the Options tab is not displayed on all each read speed type in the Specifications appendix.
instruments. Note that Sweep is not available on the NB model.
6. Toggle to set the plate type: 6-, 12-, 24-, 48-, 96, or 384*-well microplates.
7. Note: Only full plate reads are supported on the touchscreen. To read a partial plate, you must
use Gen5.
8. If applicable, tap the Blank tab to add blank wells.

a. In the Blank Well screen, use the keypad to enter which well should be blank, then
tap OK.
b. Tap Add to enter more blank wells, up to 12.
9. If the reader is equipped with shake capability, tap Options and define a shake step, if
desired. Set the shake’s duration using the keypad and intensity by toggling through the
options.
10. Tap Save. The protocol now appears in the protocol list.
*Not all instruments support 384-well microplates.

Start a Protocol
For instruments equipped with incubation capability: To perform incubation during

measurement, either turn on incubation from the Incubate tab (Quick Menu) or tap the
temperature to the left of the Run Protocol (in a defined protocol).
The onboard storage for the touchscreen can accommodate data for up to 12
microplate reads. The data is stored by date and time, not by filename. If 12 results are
already in onboard storage, the next read data saved will overwrite the oldest of the
saved results.
Note that only plate read results are saved. System Test and Absorbance Plate Test
results are not saved; they can only be exported to USB flash drive or printed.
1. From the Main Menu, tap the protocol you

want to run. The Run Protocol screen opens,
displaying the protocol’s parameters.
2. Place the microplate on the carrier, and tap

Start. When the read is finished, the results are
displayed. Tap Output to send results to the
printer or USB flash drive.
Change the Protocol Display Order
1. From the Main Menu, tap Instrument, then tap the Options tab.
2. In the Protocol display order field, select Alphabetically or Last accessed first.
Edit, Delete, or Copy a Protocol

Edit a Protocol
1. From the Main Menu, tap Protocol.

2. Tap the protocol that you want to modify, then tap Edit.
3. Make any desired changes, then tap Save.

Delete a Protocol
1. From the Main Menu, tap Protocol.

2. Tap the protocol you want to delete, then tap Delete.
Copy a Protocol
Copying an existing protocol and then editing it is a quick way to create a new protocol.
1. In the Main Menu, select the protocol you want to copy, then tap Copy.
2. You are prompted to enter a name for the copied protocol.
3. Make any desired changes, then tap Save.
View or Output Results Stored on the Reader
BioTek supplies a blank USB flash drive with the instrument if you choose to output the
system test or assay results.
1. In the Main Menu, tap Results, then select the date/time of the read for which you
want to view or output results. The results are displayed on the touchscreen.
2. Tap Output. The results are printed and/or saved to the USB flash drive, depending
on the output format you selected (see page 34).
Operate the Reader Using Gen5 Software
Gen5 RC (Reader Control) software is supplied with the 800 TS. This edition supports
only instrument control and data reporting/exporting. To perform kinetic reads, data
reduction, and custom exports, a software upgrade is required; contact BioTek
Customer Care.
BioTek Gen5 software supports all 800 TS reader models. Use Gen5 to control the reader;
perform data reduction and analysis on the measurement values; print or export results;
and more. This section provides brief instructions for working with Gen5 to create
protocols and experiments and read plates. Refer to the Gen5 Help system for more
information.

Operate the Reader Using Gen5 Software | 39
Verify the Filter Table
filter wheel.
1. From the Gen5 main screen, select System > Instrument Configuration, highlight the
800 TS, and click View/Modify.
2. Click Setup, and then click the Absorbance tab.
3. Verify that the values match the contents of the filter wheel. See the Installation
chapter for instructions for accessing the filter wheel.
4. To change the setting for a filter wheel position, enter the new value and click Send
Wavelengths.
5. Click Close when you are finished.
Protocols and Experiments

In Gen5, a protocol contains instructions for controlling the reader and (optionally)
instructions for analyzing the data retrieved from the reader. At a minimum, a protocol
must specify the procedure for the assay you wish to run. After creating a protocol, create
an experiment that references the protocol. You’ll run the experiment to read plates and
analyze the data.
These instructions briefly describe how to create a protocol in Gen5. See the Gen5 Help
system for complete instructions.
1. In the Gen5 Task Manager, select Protocol > Create New.

2. Open the Procedure dialog. If prompted to select a reader, select the 800 TS and click
OK.
3. Select a Plate Type.
Gen5 stores measurements and other characteristics for individual plate types in a
database. It is essential that you select (or define) the plate type to match the assay
plate. Otherwise, results may be invalid. See the "Plate Type Database" topic in the
Gen5 help for instructions.
4. Add steps to the procedure for reading, shaking, or heating the plate and more. Click
Validate to verify that the reader supports the defined steps, and then click OK.

5. Optionally, perform any of these steps to analyze and report the results:
l Open the Plate Layout dialog and assign blanks, samples, controls, and/or
standards to the plate.
l Open the Data Reduction dialog to add data reduction steps. Categories include
Transformation, Well Analysis, Curve Analysis, and Qualitative Analysis.
l Create a report or export template via the Report/Export Builders.
6. Select File > Save and give the file an identifying name.
These instructions briefly describe how to create an experiment and then read a plate in
Gen5. See the Gen5 Help system for complete instructions.
1. In the Gen5 Task Manager, select Experiment > Create using an existing protocol.
2. Select the desired protocol and click OK.
3. Select a plate in the menu tree and click .

4. When the read is complete, measurement values appear in Gen5. Select the desired
data set from the Data list.
5. Select File > Save and give the file an identifying name.
Plate Shaking Options

The 800 TS supports linear plate shaking, with user-selected amplitude from 1 mm to 6
mm, in 1 mm steps. Frequency ranges from about 18 Hz to about 6 Hz, depending on the
selected amplitude. Shaking is controlled using Gen5 by adding a Shake step to a protocol's
procedure.

Recommendations for Optimum Performance | 41
Gen5 Shake Step options
Recommendations for Optimum Performance
General
l Microplates should be clean and free from dust or bottom scratches. Use new
microplates from sealed packages. Do not allow dust to settle on the surface of the
solution; use microplate covers or seals when not reading the plate. Filter solutions to
remove particulates that could cause erroneous readings.
l Although the 800 TS supports standard flat, U-bottom, and V-bottom microplates, the
reader achieves optimum performance with flat-bottomed wells. See Appendix A,
Specifications for more information on the supported plates.
l Non-uniformity in the optical density of the well bottoms can cause loss of accuracy,
especially with U- and V-bottom polyvinyl microplates. Check for this by reading an
empty microplate. Dual wavelength readings can eliminate this problem, or bring the
variation in density readings to within acceptable limits for most measurements.
l Inaccuracy in pipetting has a large effect on measurements, especially if smaller
volumes of liquid are used. For best results in most cases, use at least 100 µL per well
in a 96-well plate and 25 µL in a 384-well plate.
l Pipetting solution into 384-well plates often traps air bubbles in the wells, which may
result in inaccurate readings. A dual-wavelength reading method usually eliminates
these inaccuracies. For best results, however, remove the air bubbles by degassing
the plate in a vacuum chamber or spinning the plate in a centrifuge before reading.
l The inclination of the meniscus can cause loss of accuracy in some solutions,
especially with small volumes. Shake the microplate before reading to help bring it
within acceptable limits. Use Tween 20, if possible (or some other wetting agent) to
normalize the meniscus for absorbance measurements. Some solutions develop

menisci over a period of several minutes. This effect varies with the brand of
microplate and the solution composition. As the center of the meniscus drops and
shortens the light path, the density readings change. The meniscus shape will stabilize
over time.
l It is the user’s responsibility to understand the volumetric limits of the plate type in
use as it applies to the assay being run.
l Use of liquids with concentrations of acids, corrosives, or solvents of 3% and greater
can begin attacking the materials inside the instrument's chamber. Running multiple
plates with concentrations < 3% in long kinetic experiments may also have a
destructive effect. If the experiment is incubated, it will accelerate the deterioration
of chamber components. When in doubt about the use of acids, corrosives, or
solvents, please contact TAC@biotek.com.
Using 384-Well Microplates

When using a 384-well microplate (NB models only), you can use the Gen5 Auto Map
feature to ensure you are using an accurate plate map for your reads. See the Gen5 Help
for more information.
Incubation and Partial Plates

Partial plate reads can be defined only through Gen5, not using the touchscreen. When
performing a partial plate read that includes an incubation step, the following
recommendations can reduce the effects of evaporation of your samples:
l Use microplate lids.
l Fill unused wells with fluid.
l Cluster your sample wells rather than spacing them throughout the plate.
l Place your sample wells in the center of the plate. This placement may lead to less
evaporation than if you place the samples in wells on the edge of the plate.

Chapter 4
Maintenance
This chapter provides instructions for cleaning and decontaminating the 800
TS.
Maintenance Overview 44
Warnings and Precautions 45
Clean Exposed Surfaces 45
Clean the Touchscreen 46
Decontamination 47
Filter Storage and Handling 49
Replacing and Aligning the Bulb 49
44 | Chapter 4: Maintenance
Maintenance Overview
A general maintenance regimen for all 800 TS models includes periodically cleaning all
exposed surfaces and decontaminating the instrument before storage or shipment. This
chapter includes instructions for the following:
l Routine cleaning procedure, page 45
l Cleaning the touch screen, page 46
l Decontamination, page 47
l Filter storage and handling, page 49
l Replacing and aligning the bulb, page 49
Required Materials
l Mild detergent
l Deionized or distilled water
l Clean, lint-free cotton cloths
l Sodium hypochlorite (NaClO, or bleach) (decontamination only)
l Safety glasses
l Surgical mask
l Protective gloves
l Lab coat
l Biohazard trash bags
l 125-mL beakers
l Cotton swabs or paper towels

Warnings and Precautions | 45
Warnings and Precautions

Read the following before performing any maintenance procedures:
Warning! Internal Voltage. Turn off and unplug the instrument for all maintenance
and repair operations.
Important! Do not immerse the instrument, spray it with liquid, or use a dripping-
wet cloth on it. Do not allow water or other cleaning solution to run into the
interior of the instrument. If this happens, contact the BioTek Service Department.
Do not soak the touchscreen! This will cause damage. Moisten a clean cloth with
deionized or distilled water and wipe the touchscreen. Dry immediately with a
clean, dry cloth.
Important! Do not apply lubricants to the microplate carrier or carrier track.
Lubricant attracts dust and other particles, which may obstruct the carrier path and
cause errors.
Warning! Wear protective gloves when handling contaminated instruments. Gloved
hands should be considered contaminated at all times; keep gloved hands away
from eyes, mouth, nose, and ears.
Warning! Mucous membranes are considered prime entry routes for infectious
agents. Wear eye protection and a surgical mask when there is a possibility of
aerosol contamination. Intact skin is generally considered an effective barrier
against infectious organisms; however, small abrasions and cuts may not always be
visible. Wear protective gloves when handling contaminated instruments.
Clean Exposed Surfaces
This procedure is for the housing of the 800 TS instrument. See "Clean the Touch
Screen" on page 46 for the cleaning procedure for the touch screen.
A regular cleaning regimen is recommended to keep the instrument free from dust and
particulates that can cause erroneous readings. Exposed surfaces may be cleaned (not
decontaminated) with a cloth moistened (not soaked) with water or water and a mild
detergent.

1. Turn off and unplug the instrument from the power supply.
2. Wet a clean cotton cloth with water, or with water and mild detergent, then thoroughly
wring out the cloth so that liquid does not drip from it.
3. Wipe the plate carrier, the inside of the plate carrier door (if equipped), and all exposed
surfaces of the instrument.
4. If detergent was used, wipe all surfaces with a cloth moistened with water.
5. Use a clean, dry, lint-free cloth to dry all wet surfaces.
If liquid is spilled inside the reader, call BioTek TAC for cleanup instructions.
Clean the Touchscreen
Important! Never spray solutions directly on the touchscreen.
Materials
Use the following products to safely clean the touchscreen:
l Dish soap or other mild cleaner
l Lintfree disposable towels
Avoid the following cleaning products:
l Strong solvents, such as alcohol, acetone, ammonium chloride, methylene

chloride, and hydrocarbons, which can permanently damage the surface of
the touchscreen.
l Fibrous materials, such as paper towels, which can scratch the
touchscreen. Over time, dirt particles and cleaning agents can get trapped
in the scratches.
Procedure
Important! Never spray solutions directly on the touchscreen.

Decontamination | 47
1. Turn off and unplug the instrument.

2. Moisten a clean, lint-free disposable cloth with water, or with water and mild detergent,
then thoroughly wring it out so that liquid does not drip from it. Do not soak the cloth.
3. Wipe the touch screen gently with the moist cloth.
4. If detergent was used, wipe the touchscreen with a cloth moistened with water.
5. Dry the screen gently using another cloth.
Decontamination
Any laboratory instrument that has been used for research or clinical analysis is considered
a biohazard and requires decontamination prior to handling.
Decontamination minimizes the risk to all who come into contact with the instrument
during shipping, handling, and servicing. Decontamination is required by the U.S.
Department of Transportation regulations.
Persons performing the decontamination process must be familiar with the basic setup and
operation of the instrument.
Turn off and unplug the instrument for the decontamination procedure.
BioTek Instruments, Inc., recommends the use of the following decontamination

solutions and methods based on our knowledge of the instrument and
recommendations of the Centers for Disease Control and Prevention (CDC). Neither
BioTek nor the CDC assumes any liability for the adequacy of these solutions and
methods. Each laboratory must ensure that decontamination procedures are
adequate for the biohazard(s) they handle.
Wear prophylactic gloves when handling contaminated instruments. Gloved hands
should be considered contaminated at all times; keep gloved hands away from
eyes, mouth, and nose. Eating and drinking while decontaminating instruments is
not advised.
Mucous membranes are considered prime entry routes for infectious agents. Wear
eye protection and a surgical mask when there is a possibility of aerosol
contamination. Intact skin is generally considered an effective barrier against
infectious organisms; however, small abrasions and cuts may not always be visible.
Wear protective gloves when performing the decontamination procedure.

Decontaminating the Reader Housing
Required Materials
l Sodium hypochlorite (NaClO, or bleach)

l 70% isopropyl alcohol (as an alternative to bleach)
l Safety glasses
l Surgical mask
l Protective gloves
l Lab coat
l Biohazard trash bags
l 125-mL beakers
l Clean, lint-free cotton cloths
Procedure
1. Turn off and unplug the reader from the power supply.
2. Prepare an aqueous solution of 0.5% sodium hypochlorite (NaClO, or bleach). If the
effects of bleach are a concern, 70% isopropyl alcohol may be used.
Check the percent NaClO of the bleach you are using. Commercial bleach is typically
10.0% NaClO; prepare a 1:20 dilution. Household bleach is typically 5.0% NaClO;
prepare a 1:10 dilution.
3. Moisten a clean, lint-free cloth with the bleach solution, then thoroughly wring it out so
that liquid does not drip from it. Do not soak the cloth.
4. Wipe the plate carrier and all exposed surfaces of the instrument, except the touch
screen (if equipped).
5. Allow the instrument to dry for 20 minutes for thorough decontamination by the bleach.
6. Moisten a cloth with deionized or distilled water and wipe all surfaces of the instrument
that have been cleaned with the bleach solution.

Filter Storage and Handling | 49
7. Use a clean, dry lint-free cloth to dry all wet surfaces.

8. Discard the used gloves and cloths, using a biohazard trash bag and an approved
biohazard container.
Decontaminating the Touchscreen

When decontaminating the 800 TS, as described on page 47, do not spray the bleach
solution on the touchscreen. Avoid fibrous materials that can scratch the surface. Do not
use a stronger bleach solution or cleaning solvent than recommended.
Filter Storage and Handling

To properly store interference filters during extended periods of non-use, package the
filters in a light-tight envelope or container, away from high humidity. This will ensure the
longest life for the filters. When handling the filters, keep the surfaces clean from
fingerprints and debris by simply wiping with a lens tissue or other lint-free cloth.
When changing or replacing filters, it is critical that the filters be placed in the
filter wheel in the correct orientation, with the light-direction arrow pointing
downward. Also, the reader or Gen5 software filter table must exactly match the
contents of the filter wheel.
Replacing and Aligning the Bulb

The tungsten or halogen bulb used on the various models of the 800 TS is user-replaceable.
The intensity of each type of bulb will slowly drop over time until the start-up self-check
detects a low signal level and the instrument displays an error code. The bulb should be
replaced and aligned at this time.
l For the 800TS and 800TSI models, order replacement bulb kit PN 1560532.
l For the 800TSUV, 800TSSUVI, and 800TSNB models, order replacement bulb
kit PN 1560533.
The kits contain complete instructions for replacing and aligning the bulb.
Warning! The bulb will be hot immediately after the reader has been shut down.
Allow the bulb to cool for at least 10 minutes before attempting to replace it.
Warning! The alignment procedure requires you to observe the light path while
the bulb is turned on. To prevent possible vision impairment, avoid looking
directly at the bulb while it is on.


Chapter 5
Instrument Qualification Process
This chapter describes the tests that BioTek Instruments, Inc., has developed
for complete qualification of all models of the 800 TS. This chapter
introduces the various test methods, describes the materials and protocol
parameters used to execute the tests, explains how to analyze test results,
and provides troubleshooting tips in the event of a failure. Instrument
Qualification Procedures, starting on page 61, contains the actual step-by-
step test procedures.
Instrument System Test 52

Absorbance Testing 52
52 | Chapter 5: Instrument Qualification Process
Instrument System Test

Each time the 800 TS is turned on, it automatically performs a series of tests on the
reader’s motors, lamp, and optical systems. If all tests pass, the microplate carrier moves
to its forward position and the Main Menu appears on the touchscreen.
If any test results do not meet the internally coded Failure Mode Effects Analysis (FMEA)
criteria established by BioTek, the reader beeps repeatedly and an error message appears
on the touchscreen. If this occurs, tap OK on the touchscreen to stop the beeping. If
necessary, initiate another system test using Gen5 or the touchscreen to try to retrieve an
error code from the reader.
Refer to Error Codes, starting on page 81, for information on error codes and
troubleshooting tips.
Refer to Sample Reports, starting on page 89, to see a sample System Test Report for 800
TS.
Absorbance Testing
BioTek developed a series of tests for the absorbance system using a combination of solid
state Absorbance Test Plates and liquid plates. The test plates and the materials used for
creating the liquid plates are available for purchase from BioTek.
To qualify the absorbance system for the 800 TS, you should perform:
l Absorbance Liquid Test 1 and Absorbance Plate Test (using BTI #7260522) or
l Absorbance Liquid Test 2
Optionally, to qualify operation in the UV range, you should also perform:
l Absorbance Liquid Test 3 or Absorbance Plate Test at 340 nm (using BTI #7260551)
BioTek Absorbance Test Plates

Absorbance Test Plate PN 7260522 uses NIST-traceable neutral density filters to confirm
absorbance specifications in the visible range (400–800 nm). This test plate also contains
precision-machined holes to verify mechanical alignment. Absorbance Test Plate PN
7260551 uses NIST-traceable neutral density filters to confirm absorbance specifications in
the UV range (340 nm).
Every test plate comes with a Test Plate Calibration Certificate, containing a table with
Absorbance OD Standards for each filter at each wavelength supported by the plate.
Before the Absorbance Plate Test can be performed, the OD Standard values must be
defined on the reader via the touchscreen or entered into Gen5. Enter and save these
values once initially, and then update them annually when the test plate is recertified by
BioTek.

Absorbance Testing | 53
Check the calibration due date on the test plate's label. If the test plate is overdue for
recalibration, contact BioTek to schedule service.
Test Method
The Absorbance Plate Test is conducted using the 800 TS touchscreen or Gen5 software
(System > Diagnostics > Test Plates) to confirm mechanical alignment and optical density
accuracy, linearity, and repeatability. When complete, a results report displays Pass or Fail
for each individual test.
l Alignment: The test plate has precisely machined holes to confirm mechanical
alignment. The amount of light that shines through these holes is an indication of
whether the microplate carrier is properly aligned with the absorbance optical path.
A reading of more than 0.015 OD for any of the designated alignment holes indicates
that the light is being “clipped” and the reader may be out of alignment.
l Accuracy: The test plate contains NIST-traceable neutral-density glass filters of known
OD values at one or more wavelengths. Actual measurements are compared against
the expected values provided in the test plate’s data sheet. Since there are several
filters with differing OD values, the accuracy across a range of ODs can be
established. Once it is proven that the reader is accurate at these OD values, the
reader is also considered to be linear. To further verify this, you can perform a linear
regression analysis on the test plate OD values in a program such as Microsoft Excel;
an R2 value of at least 0.9900 is expected.
l Repeatability: This test ensures the instrument meets its repeatability specification
by conducting repeated reads of each neutral-density filter on the test plate and
comparing the results.
Sample Test Report

Refer to Sample Reports, starting on page 89, to see a sample Absorbance Plate Test
Report for 800 TS.
Troubleshooting
If a test fails, try the troubleshooting tips below. If the test continues to fail, contact BioTek
TAC.
Important! Do not remove filters from the Absorbance Test Plate. Do not use alcohol or
other cleaning agents, and do not touch the filters with your bare fingers.
If a higher-OD well reports "#N/A" for Min/Max Limit and Result, the measured OD is
beyond the specified range for Accuracy or Repeatability used with this test, and
therefore no pass/fail determination is made. It does not indicate a test failure.

Alignment Test
l Ensure that the test plate is properly seated in the microplate carrier.
l Check the four alignment holes (B2, B12, G1, G11) to ensure they are clear of debris.
l Check the microplate carrier to ensure it is clear of debris.
Accuracy Test
l Check the neutral-density filters to ensure they are clean (positions C1, D4, E2, F5, G3,
H6). If needed, clean the filters with lens paper. Do not remove any filters, and do not
use alcohol or other cleaning agents.
l Verify that the wavelength/expected OD values entered for the plate on the reader or
in Gen5 match the information provided on the test plate's data sheet.
Repeatability Test
l Check the neutral-density filters to ensure there is no debris that may have shifted
between readings and caused changes.
l Check the microplate carrier to ensure it is clear of debris.
Absorbance Liquid Tests

BioTek Instruments, Inc., has developed a series of liquid test procedures for testing your
reader's absorbance system.
Test Methods
Absorbance Liquid Test 1 confirms repeatability and alignment of the reader when a
solution is used in the microplate. If these tests pass, then the lens placement and optical
system cleanliness are proven. For the Repeatability portion of this test, two columns
containing a color-absorbing solution are read five times at 405 nm. For each well, an
"allowed deviation" is determined based on its Mean OD and the reader's repeatability
specification. Each well's Standard Deviation must be less than its Allowed Deviation to
pass. To confirm the reader’s mechanical alignment, the plate is rotated 180 degrees in
the carrier (e.g., A1 is now in the H12 position) and the same two columns are read. The
initial and new OD readings are compared, using the reader's accuracy specification. If the
two readings in the same well do not meet specification, the reader may be out of
alignment.
If an Absorbance Test Plate is not available, Absorbance Liquid Test 2 may be conducted
to test the instrument's alignment, repeatability, and accuracy by preparing a series of
solutions of varying OD values as described on page 71.

Absorbance Liquid Test 3 is an optional test offered for those sites that must have proof of
linearity at 340 nm. (Alternatively, the BioTek 340 nm Absorbance Test Plate may be used;
see page 52.) This test is optional since the 800 TS has good “front end” linearity
throughout the specified wavelength range. While the absolute values of the OD cannot be
determined by this test, the results will indicate if there is adequate repeatable absorbance
and a linear slope. This method is dependent upon proper dye dilution and a skilled
pipetting technique. It is expected that the first dilution (mid-level solution) will have an
absorbance value near 75% of that of the stock (high-level) solution, and that the second
dilution (low-level solution) will have an absorbance value near 50% of that of the stock
solution.
Gen5 Protocol Parameters

If using Gen5 to conduct the absorbance liquid tests, follow these instructions. The
information in this section represents the recommended reading parameters for the
referenced Gen5 protocol(s). It is possible that your tests will require modifications to some
of these parameters, such as the Plate Type.
The Plate Type setting in each Gen5 protocol should match the actual 96-well plate in
use.
800 TS Abs Test 1.prt
Parameter Setting
Plate Type 96 WELL PLATE

Two Read Steps
Set a Run Time/Interval combination to read the
Kinetic loop (one per Read step)
plate five times with minimal delay
Detection Method Absorbance
Read Type Endpoint
First Read step: A1..H2
Read wells
Second Read step: A11..H12
Wavelength 405 nm
Read Speed Normal
Delay after plate movement 100 msec
Plate Out,In step between loops Text "rotate the plate 180 degrees"

Parameter Setting

Two Read Steps
Read Type Endpoint
First Read step: "Normal"
Step labels
Second Read step: "Turnaround"
Read wells Full plate
Wavelength 2 (450 nm, 630 nm)
Read Speed Normal
Define two Delta OD transformations (450 – 630
Data Reduction
nm), one per Read data set
Parameter Setting

Shake Step (if supported) Linear, 4 minutes, default frequency
Two Read Steps
Read Type Endpoint
Read wells A1..H6
Wavelength 340 nm
Read Speed Normal

Results Analysis
The Absorbance Liquid Test procedures begin on page 68. All tests are conducted using the
Normal read speed.
Absorbance Liquid Test 1
Accuracy Specification:
± 1.0% ± 0.010 OD from 0.000 to 2.000 OD
Repeatability Specification:
± 0.5% ± 0.005 OD from 0.000 to 2.000 OD
1. The plate is read five times in the “Normal” position at 405 nm. Calculate the Mean OD
and Standard Deviation of those five reads for each well in columns 1 and 2.
2. For each well in columns 1 and 2, calculate the Allowed Deviation using the
Repeatability specification for a 96-well plate (Mean OD x 0.05 + 0.005). For each well,
its Standard Deviation should be less than its Allowed Deviation.
Example: Five readings in well A1 of 0.802, 0.802, 0.799, 0.798, and 0.801 result in a
Mean of 0.8004 and a Standard Deviation of 0.0018. The Mean multiplied by 0.5%
(0.8004 * 0.005) equals 0.004, and when added to 0.005 equals 0.009; this is the Allowed
Deviation for well A1. Since the Standard Deviation for well A1 is less than this value,
the well meets the test criteria.
3. The plate is read five times in the “Turnaround” position at 405 nm. Calculate the Mean
OD of those five reads for each well in columns 11 and 12.
4. Perform a mathematical comparison of the Mean values for each well in its Normal and
Turnaround positions (that is, compare A1 to H12, A2 to H11, B1 to G12,… H2 to A11). To
pass the test, the differences in the compared Mean values must be within the Accuracy
specification for a 96-well microplate.
Example: If the Mean value for well A1 in the Normal position is 1.902 with a specified
accuracy of ±1.0% ±0.010 OD, then the expected range for the Mean of the well in its
Turnaround (H12) position is 1.873 to 1.931 OD. 1.902 x 0.010 + 0.010 = 0.029; 1.902 -
0.029 = 1.873; 1.902 + 0.029 = 1.931.

Accuracy Specification:
+/-1.0% +/-0.010 OD from 0.000 to 2.000 OD
+/-0.5% +/-0.005 OD from 0.000 to 2.000 OD

1. The plate is read five times at 450/630 nm ("Normal" position), resulting in five sets of
Delta OD data. Calculate results for Linearity:
l Calculate the mean absorbance for each well, and average the means for each
concentration.
l Perform a regression analysis on the data to determine if there is adequate
linearity. Since it is somewhat difficult to achieve high pipetting accuracy when
conducting linear dilutions, an R2 value of at least 0.9900 is considered adequate.
2. Calculate the results for Repeatability:
l Calculate the Mean and Standard Deviation for the five readings taken at each
concentration. Only one row of data needs to be analyzed.
l For each Mean, calculate the Allowed Deviation using the Repeatability
specification for a 96-well plate of ±0.5% ±0.005 OD.
l The Standard Deviation for each set of readings should be less than the Allowed
Deviation.
Example: Readings of 1.950, 1.948, 1.955, 1.952, and 1.950 will result in a Mean of
1.951, and a Standard Deviation of 0.0026. The Mean (1.951) multiplied by 0.5%
(1.951 x 0.005) = 0.009755, which, when added to the 0.005 (0.009755 + 0.005) =
0.0148 OD, which is the Allowed Deviation. Since the Standard Deviation is less
than this value, the reader meets the test criteria.
3. After gathering data for the Linearity Test, the plate is read five more times with the A1
well in the H12 position ("Turnaround" position). This results in values for the four
corner wells that can be used to assess alignment. Calculate results for the Alignment
Test:
l Calculate the means of the wells A1 and H1 in the Normal plate position (data
from Linearity Test) and in the Turnaround position.
l Compare the mean reading for well A1 to its mean reading when in the H12
position. Next, compare the mean values for the H1 well to the same well in the
A12 position. The difference in the values for any two corresponding wells should
be within the Accuracy specification for 96-well plates. If the four corner wells are
within the accuracy range, the reader is in alignment.
Example: If the mean of well A1 in the normal position is 1.902, where the
specified accuracy is ±1.0% ±0.010 OD, then the expected range for the mean of
the same well in the H12 position is 1.873 to 1.931 OD. (1.902 x 1.0% = 0.019 +
0.010 = 0.029, which is added to and subtracted from 1.902 for the range.)


+/-1.5% +/-0.005 OD from 0.000 to 2.000 OD @ 340nm
1. The plate is read five times at 340 nm. For each well, calculate the Mean OD and
Standard Deviation of the five readings.
2. For each Mean calculated in step 1, calculate the Allowed Deviation using the
Repeatability specification for a 96-well plate (Mean OD x 0.015 + 0.005). For each well,
its Standard Deviation should be less than its Allowed Deviation.
Example: Five readings in well A1 of 0.802, 0.802, 0.799, 0.798, and 0.801 result in a
Mean of 0.8004 and a Standard Deviation of 0.0018. The Mean multiplied by 1.5%
(0.8004 * 0.015) equals 0.012, and when added to 0.005 equals 0.017; this is the Allowed
Deviation for well A1. Since the Standard Deviation for well A1 is less than this value,
the well meets the test criteria.
3. Calculate results for Linearity:
l For each of the three test solutions, calculate the average Mean OD for the wells
containing that solution (mean of wells A1 to H2, A3 to H4, and A5 to H6).
l Perform a regression analysis on the data to determine if there is adequate
linearity. The three average Mean OD values are the “Y” values. The solution
concentrations are the “X” values (1.00, 0.75, 0.50). Since it is somewhat difficult
to achieve high pipetting accuracy when conducting linear dilutions, an R2 value of
at least 0.9900 is considered adequate.
Troubleshooting
If an absorbance liquid test fails, try the following. If a test continues to fail, contact BioTek
TAC.
l Check the microwells and plate carrier for debris that may have shifted and caused
changes.
l Ensure the microplate is properly seated in the carrier.
l As applicable, confirm that the plate was properly oriented in the "Normal" and
"Turnaround" positions.
l Liquid Test 1 can fail due to the meniscus effect, which can cause readings to
decrease over time. If you suspect this may be the case, include a shake step between
the read steps in the protocol.


Chapter 5
Instrument Qualification Procedures
This chapter contains the step-by-step procedures for verifying that the 800
TS and its various subsystems are performing to specification. Instrument
Qualification Process, starting on page 51, introduces the various test
methods, describes the materials and relevant protocol parameters used to
execute the tests, explains how to analyze test results, and provides
troubleshooting tips in the event of a failure.
Overview 62
IQ/OQ/PQ Description 62
Recommended Qualification Schedule 64
System Test 64
Absorbance Plate Test 66
Absorbance Liquid Tests 68
62 | Chapter 6: Instrument Qualification Procedures
Overview
This chapter contains BioTek Instrument's recommended qualification procedures for all
800 TS models.
Every 800 TS reader is fully tested at BioTek prior to shipment and should operate properly
upon initial setup. If you suspect that a problem occurred during shipment, if you have
received the equipment after returning it to the factory for service, and/or if regulatory
requirements dictate that you qualify the equipment on a routine basis, perform the
procedures outlined in this chapter.
See the Recommended Qualification Schedule on page 64 to determine which
qualification tests shall be conducted for your and to meet your site's regulatory
requirements.
A Product Qualification Package (PN 1560516) for the 800 TS is available for purchase.
The package contains complete procedures, Gen5 protocols, checklists, and logbooks
for performing Installation Qualification, Operational Qualification, Performance
Qualification, and Preventive Maintenance. Contact your local BioTek dealer for more
information.
IQ/OQ/PQ Description
Installation Qualification confirms that the reader and its components have been supplied
as ordered and ensures that they are assembled and configured properly for your lab
environment.
l The recommended IQ procedure consists of setting up the instrument and its
components as described in Chapter 2, Installation, and performing the System Test.
l The IQ procedure should be performed initially (before the reader is used for the first
time).
l The successful completion of the IQ procedure verifies that the instrument is installed
correctly. The Operational Qualification procedure should be performed immediately
following the successful IQ.
Operational Qualification confirms that the equipment operates according to
specification initially and over time.
l The recommended OQ procedure consists of performing the system test, absorbance
plate test, and a series of liquid tests.
l The OQ procedure should be performed initially (before first use) and then routinely;
the recommended interval is annually. It should also be performed after any major
repair or upgrade to the hardware or software.

IQ/OQ/PQ Description | 63
l Although out-of-tolerance failures will be detected by the OQ tests, results should be

compared with those from the routine Performance Qualification tests and previous
OQ tests to monitor for trends.
l The successful completion of the OQ procedure, in combination with results that are
comparable to previous PQ and OQ tests, confirms that the equipment is operating
according to specification initially and over time.
Performance Qualification confirms that the reader consistently meets the requirements
of the tests performed at your laboratory.
l The recommended PQ procedure consists of performing the system test, absorbance
plate test, and a series of liquid tests.
l Your facility’s operating policies may also require that you routinely perform an
actual assay, to confirm that the reader will consistently give adequate results for the
assays to be run on it.
l These tests should be performed routinely; the recommended interval is monthly or
quarterly, depending on the test. This frequency may be adjusted depending on the
trends observed over time.
l The successful completion of the PQ procedure confirms that the equipment is
performing consistently under normal operating conditions.

Recommended Qualification Schedule

This schedule defines BioTek-recommended intervals for qualification for an instrument
used for one shift seven days a week.
The risk and performance factors associated with your assays may require that the
Operational and Performance Qualification procedures be performed more or less
frequently than shown here.
IQ OQ PQ
Tasks/Tests Initially/
Initially Monthly Quarterly
Annually
All models:
Unpacking, installation, and setup ü
System Test ü ü ü
Absorbance Plate Test ü ü
Absorbance Liquid Test 1 or Liquid Test

ü ü
2*
(Optional, for UV models only)
Absorbance Liquid Test 3 or 340 nm
ü ü
Absorbance Plate Test (using
BTI #7260551)
* If you have Absorbance Test Plate PN 7260522, perform Liquid Test 1. Otherwise,
perform Liquid Test 2.
System Test
Instrument System Test, starting on page 20, describes this test and explains where to
find information on error codes and troubleshooting tips, as well as sample test reports
for 800 TS.
Setup
If your assays use incubation, we recommend enabling temperature control for at least
37°C and allowing the incubator to reach its set point before running the system test. To
access this feature:

System Test | 65
l On the touchscreen:
1. Tap the temperature display. Note that until Temperature Control is enabled,
three dashes are displayed.
2. If necessary, turn on Temperature Control and enter a setpoint of at least
37°C, then tap Home to return to the Main Menu.
l In Gen5, select System > Instrument Control, and click the Pre-Heating tab.
Test Procedure Using the Touchscreen
1. When the temperature display reaches the defined setpoint, tap Instrument, then tap
Options.
2. Under System test, tap Start.
3. When the test finishes, tap USB Report to save the test results to a USB flash drive,
Print to print the test results, or Exit to close the screen.
Test Procedure Using Gen5
1. From the Gen5 main screen, select System > Diagnostics > Run System Test.
If the test fails during execution, a message box appears in the Gen5. Close the box; the
test report contains the error code that was generated by the failure.
2. When the test is complete, a dialog appears, requesting additional information. Enter
any required information and then click OK.
3. The results report appears. it shows either “SYSTEM TEST PASS” or “SYSTEM TEST FAIL
*** ERROR (error code) DETECTED.”
If the test failed, look up the error code in Appendix B, Error Codes, starting on
page 81, to determine its cause. If the cause is something you can fix, turn off the
reader, fix the problem, and then turn the reader back on and retry the test. If
the test continues to fail, or if the cause is not something you can fix, contact
BioTek TAC.
4. If required, print, sign, and date the report, and store it with your test documentation.
5. If applicable, turn off the incubator.

Absorbance Plate Test
BioTek Absorbance Test Plate, starting on page 52, describes the test methods and
provides troubleshooting tips in the event of test failure.
Requirement
To perform this test, you will need:
l Absorbance Test Plate, BTI #7260522
l (Optional) 340 nm Absorbance Test Plate, BTI #7260551
l (Optional) 340 nm filter installed in the instrument
l Current Absorbance Test Plate Calibration Certificate(s)
Setup
Before an Absorbance Test Plate can be used for qualification, you must enter information
from its Calibration Certificate into the reader or Gen5. Perform these steps initially, and
then repeat them annually after the test plate is recertified by BioTek.
1. Obtain the current Test Plate Calibration Certificate.

2. From the Main Menu, tap Instrument, the tap Test Plate.
3. Tap Test Plate Certificate and enter the plate’s serial number and Next Calibration Due
date, then tap Back to return to the Test Plate tab.
4. The wavelength selection buttons reflect the filters installed in the reader. Tap each
button, and enter the OD Standard values from the Calibration Certificate into the grid.
Make sure you enter the correct value for each well/wavelength combination.
Using Gen5
1. Obtain the current Test Plate Calibration Certificate.

2. Start Gen5, and select System > Diagnostics > Test Plates > Add/Modify Plates.
3. Click Add. The Absorbance Test Plate dialog appears.

Absorbance Plate Test | 67
4. Select the appropriate Plate Type, and then enter the plate's serial number.
5. Enter the Last Certification and Next Certification dates from the calibration label on
the Test Plate.
6. If the wavelength values in the top row of the grid in Gen5 are appropriate for your
tests, enter the OD Standard values from the Calibration Certificate into the grid. Make
sure you enter the correct value for each well/wavelength combination.
If you need to change the wavelength values, click Wavelength List. Add, change, or
delete the values as needed and click OK.
7. Review all of the values that you entered. When finished, click OK to save the
information.
Test Procedure
1. Place the Absorbance Test Plate on the microplate carrier, with well A1 in the proper
location.
2. From the Main Menu, tap Instrument and then Test Plate.
3. Tap a Wavelength Selection button, then tap Start.
4. When the test is complete, choose an Output option (Print or USB Report), or tap Exit
to return to the Main Menu.
Using Gen5
1. From the Gen5 main screen, click System > Diagnostics > Test Plates > Run. If
prompted, select the desired Test Plate and click OK.
2. When the Absorbance Test Plate Options dialog appears, enter any required
information.
3. Highlight the wavelength(s) to be included in this test.
You need to select only those wavelengths most appropriate for your use of the reader.

4. (Optional) Enter a comment.

5. Click Start Test.
6. Place the Absorbance Test Plate on the microplate carrier, with well A1 in the proper
location.
7. Click OK to run the test.
8. When the test is complete, the results report appears. Scroll through the report; every
result should show “PASS”.
l Troubleshooting tips are provided on page 53.
l Test descriptions are provided on page 53.
Absorbance Liquid Tests
Absorbance Liquid Tests, starting on page 54, describes the test methods, lists the
Gen5 protocol parameters, explains how to analyze the test results, and provides
troubleshooting tips in the event of test failure.
The tests in this section require specific microplates, solutions, and filters. Your
laboratory may require a deviation from some of these tests. For example, you may
wish to use a different plate or test solution. If deviation from the tests as presented
in this section is required, perform the following steps the first time each test is run:
• Perform the tests exactly as described here.
• Rerun the tests using your particular plates, solutions, and so on.
• If the results are comparable, then the results from these tests will be your
baseline for future tests. Document your new test procedure, and save all test
results.
Materials
Manufacturer part numbers are subject to change.

Absorbance Liquid Tests | 69
l New 96-well, clear, flat-bottom microplate (Corning Costar #3590 recommended)

l Stock Solution A or B, which may be formulated by diluting a dye solution available
from BioTek (A) or from the materials listed below (B).
l If using Gen5, the protocol 800 TS Abs Test 1.prt described on page 55
Solution A
l BioTek QC Check Solution No. 1 (PN 7120779, 25 mL; or 7120782, 125 mL)
l Deionized water
l 5-mL Class A volumetric pipette
l 100-mL volumetric flask
1. Pipette a 5-mL aliquot of BioTek QC Check Solution No. 1 into a 100-mL volumetric flask.
2. Add 95 mL of DI water; cap and shake well. The solution should measure approximately
2.000 OD when using 200 µL in a flat-bottom microwell.
Solution B
l Deionized water
l FD&C Yellow No. 5 dye powder (typically 90% pure)
l Tween 20 (polyoxyethylene (20) sorbitan monolaurate) or BioTek wetting agent, PN
7773002 (a 10% Tween solution)
l Precision balance with capacity of 100 g minimum and readability of 0.001 g
l 1-liter volumetric flask
l Weigh boat
1. Weigh out 0.092 gram of FD&C No. 5 yellow dye powder into a weigh boat.
2. Rinse the contents into a 1-liter volumetric flask.
3. Add 0.5 mL of Tween 20, or 5 mL of BioTek’s wetting agent.
4. Make up to 1 liter with DI water; cap and shake well.

Test Procedure
Be sure to use a new microplate. Debris, fingerprints, or scratches may cause

variations in readings.
1. Using freshly prepared stock solution (Solution A or B), prepare a 1:2 dilution using
deionized water (one part stock, one part deionized water; the resulting solution is a 1:2
dilution). The concentrated stock solution should have an optical density of
approximately 2.000 OD or lower.
2. Pipette 200 µL of the stock solution into column 1.
3. Pipette 200 µL of the diluted solution into column 2.
After pipetting the diluted test solution into the microplate and before reading
the plate, we strongly recommend shaking the plate for four minutes. This will
allow any air bubbles in the solution to settle and the meniscus to stabilize.
Alternatively, wait 20 minutes after pipetting the test solution before reading the
plate.
If using the touchscreen:
4. Read the microplate five times at 405 nm using the Normal Read Speed. When
prompted, rotate the plate 180 degrees and continue.
5. Print the five sets of raw data or send it to the USB flash drive for use in another
program.
6. Perform the Results Analysis calculations described in Chapter 5.
If using Gen5:
4. Create a Gen5 experiment based on the 800 TS Abs Test 1 protocol and read the plate.
When prompted, rotate the plate 180 degrees and continue.
5. When the experiment is finished:
l Save the experiment. Refer to the instructions on page 57 to perform calculations
and determine pass/fail.
l Test descriptions are provided on page 54

The recommended method for testing the instrument’s alignment, repeatability, and
accuracy is to use Absorbance Test Plate BTI #7260522 (see page 52). If the test plate is
not available, however, Liquid Test 2 can be used for these tests.
Materials
l A new 96-well, clear, flat-bottom microplate (Corning Costar #3590 is recommended)

l Ten test tubes, numbered consecutively, set up in a rack
l Calibrated hand pipette (Class A volumetric pipette recommended)
l Solution A or B (see the instructions for Liquid Test 1)
l A 0.05% solution of deionized water and Tween 20
l If using Gen5, the protocol 800 TS Abs Test 2.prt, described on page 56
Test Procedure
1. Create a percentage dilution series, beginning with 100% of the original concentrated
stock solution (A or B) in the first tube, 90% of the original solution in the second tube,
80% in the third tube, all the way to 10% in the tenth tube. Dilute using the 0.05%
solution of deionized water and Tween 20. This solution can also be made by diluting the
BioTek wetting agent 200:1.
Tube Number 1 2 3 4 5 6 7 8 9 10
Volume of Original Concentrated

20 18 16 14 12 10 8 6 4 2
Solution (mL)
Volume of 0.05% Tween Solution

0 2 4 6 8 10 12 14 16 18
(mL)
Absorbance expected if original

2.0 1.8 1.6 1.4 1.2 1.0 0.8 0.6 0.4 0.2
solution is 2.0 at 200 μL

The choice of dilutions and the absorbance of the original solution can be varied. Use
this table as a model for calculating the expected absorbances of a series of dilutions,
given a different absorbance of the original solution.
2. Pipette 200 µL of the concentrated solution from Tube 1 into each well of the first
column, A1 to H1, of a new flat-bottom microplate.
3. Pipette 200 µL from each of the remaining tubes into the wells of the corresponding
column of the microplate (Tube 2 into wells A2 to H2, Tube 3 into wells A3 to H3, and so
on).
plate.
4. Read the microplate five times at 450 nm (Primary)/630 nm (Secondary) using the
Normal Read Speed.
5. Do not discard the plate. You will use it for the Alignment Test.
6. Print the five sets of raw data or send it to the USB flash drive for use in Excel or other
program.
7. Perform the Linearity and Repeatability calculations described in Chapter 5.
8. (Alignment Test) Rotate the plate 180 degrees in the carrier, and then read the plate
five more times, with the same protocol as described in step 4. This test results in values
for the four corner wells that can be used to determine alignment.
9. Perform the Alignment calculations described in Chapter 5.
If using Gen5:
4. If using Gen5, create an experiment based on the 800 TS Abs Test 2 protocol and read
the plate. When prompted, rotate the plate 180 degrees.
5. When finished:


l Test descriptions are provided on page 54.
Absorbance Liquid Test 3 (optional)
Absorbance Liquid Test 3 is provided for sites requiring proof of linearity at 340 nm.
This test is optional because the 800 TS has good "front end" linearity throughout its
wavelength range. As an alternative, the 340 nm Absorbance Test Plate (BTI #7260551)
may be used for this test.
This test verifies operation of the reader at 340 nm.
Materials
l 340 nm filter installed in the reader

l New 96-well, clear, flat-bottom microplate (Corning Costar #3590 recommended)
l Calibrated hand pipette(s)
l Beakers and graduated cylinder
l Precision balance with readability to 0.01 g
l Buffer solution described below
l If using Gen5, the protocol 800 TS Abs Test 3.prt, described on page 56
Buffer Solution
l Deionized water
l Phosphate-Buffered Saline (PBS), pH 7.2–7.6, Sigma tablets, #P4417 (or equivalent)
l β-NADH Powder (β-Nicotinamide Adenine Dinucleotide, Reduced Form) Sigma bulk
catalog number N 8129, or preweighed 10-mg vials, Sigma number N6785-10VL (or
BioTek PN 98233). Store the powder according to the guidelines on its packaging.

1. Prepare a PBS solution from the Sigma tablets.

2. In a beaker, mix 50 mL of the PBS solution with 10 mg of the β-NADH powder and mix
thoroughly. This is the 100% Test Solution.
Test Procedure
1. Prepare the 75% Test Solution by mixing 15 mL of the 100% Test Solution with 5 mL of
the PBS Solution.
2. Prepare the 50% Test Solution by mixing 10 mL of the 100% Test Solution with 10 mL of
the PBS Solution.
3. Carefully pipette the three solutions into a new 96-well microplate:
l 150 µL of the 100% Test Solution into all wells of columns 1 and 2
l 150 µL of the 75% Test Solution into all wells of columns 3 and 4
l 150 µL of the 50% Test Solution into all wells of column 5 and 6
plate.
4. Read the microplate five times at 340 nm using the Normal Read Speed.
5. Print the five sets of raw data or send it to the USB flash drive for use in other program.
6. Perform the Results Analysis calculations described in Chapter 5.
If using Gen5:
4. Create a Gen5 experiment based on the 800 TS Abs Test 3 protocol and read the plate.

Appendix A
Specifications
This appendix contains BioTek's published specifications for the 800 TS.
General Specifications 76
Absorbance Specifications 77
76 | Appendix A: Specifications
General Specifications
Microplates
The 800 TS accommodates standard 6-, 12-, 24-, 48-, 96-, and (with the NB model) 384-well microplates
with 128 x 86 mm geometry and, if using Gen5 software with the NB model, 60-, 75-, and 96-well
Terasaki plates and BioCell vessels.
Maximum plate height: 0.90" (22.86 mm)

Hardware and Environmental
800TS/800TSI models: Tungsten gas-filled bulb.
Light Source
800TSUV/800TSUVI/800TSNB models: Halogen bulb
Dimensions 16.5" D x 15" W x 7" H (41.9 x 38.1 x 17.8 cm)
Weight: < 22 lbs (10 kg)
Environment: Operational temperature range: 18° to 40°C (64°F to 104°F)
Humidity: 10% to 85% relative humidity (non-condensing)
Power Supply: 24-volt DC power supply compatible with 100–240 V AC @50–60 Hz
40W maximum, non-incubated models; 150W maximum, incubated
Power Consumption:
models
Temperature control for 800TSI model: 6°C over ambient to 50°C.
Incubation: Temperature control for 800TSUVI model: 8°C over ambient to 50°C
Temperature stability and uniformity is ± 0.5°C across the plate @ 37°C.

Absorbance Specifications | 77
Absorbance Specifications
Standard Models (800TS/800TSI)

Wavelength Range: 400 nm to 750 nm
Absorbance Measurement Range

Normal Read Mode: 0.000 to 4.000 OD
Rapid Read Mode: 0.000 to 4.000 OD
Sweep Read Mode: 0.000 to 3.000 OD
Absorbance Resolution
0.001 OD when operated in standalone mode
0.0001 OD when operated with Gen5
Accuracy
Normal Read Mode: ±1.0% ±0.010 OD from 0.000 to 2.000 OD @ 405 nm
Rapid Read Mode: ±2.0% ±0.020 OD from 0.000 to 2.000 OD @ 405 nm
Sweep Read Mode: ±1.0% ±0.020 OD from 0.000 to 1.000 OD @ 405 nm
Linearity
Normal Read Mode: ±1.0% ±0.010 OD from 0.000 to 2.000 OD @ 405 nm
±3.0% ±0.010 OD from 2.000 to 3.000 OD @ 450 nm

Rapid Read Mode: ±2.0% ± 0.010 OD from 0.000 to 2.000 OD @ 405 nm
Sweep Read Mode: ± 1.0% ± 0.010 OD from 0.000 to 1.000 OD @ 405 nm
Repeatability (STD)
Normal Read Mode: ± 0.5% ± 0.005 OD from 0.000 to 2.000 OD @ 405 nm
Rapid Read Mode: ± 1.0% ± 0.010 OD from 0.000 to 2.000 OD @ 405 nm
Sweep Read Mode: ± 2.0% ± 0.020 OD from 0.000 to 1.000 OD @ 405 nm
Throughput
From carrier start to carrier stop:

96-Well, Single Wavelength, Normal Read Mode: 39 seconds
96-Well, Dual Wavelength, Normal Read Mode: 73 seconds
96-Well, Single Wavelength, Rapid Read Mode: 26 seconds
96-Well, Single Wavelength, Sweep Read Mode: 18 seconds
Ultraviolet (UV) Models (800TSUV/800TSUVI)


Normal and Rapid Read Mode (340 nm–399 nm): 0.000 to 4.000 OD
Normal and Rapid Read Mode (400 nm–750 nm): 0.000 to 4.000 OD
Sweep Read Mode (400 nm–750 nm): 0.000 to 3.000 OD
Accuracy (340 nm to 399 nm)

Normal Read Mode: ±2.0% ± 0.010 OD from 0.000 to 2.000 OD @ 340 nm
Accuracy (400 nm to 750 nm)

Sweep Read Mode: ±1.0% ±0.020 OD from 0.000 to 1.000 OD @ 405 nm
Linearity (340 nm to 399 nm)


Absorbance Specifications | 79
Linearity (400 nm to 750 nm)

±3.0% ± 0.010 OD from 2.000 OD to 3.000 OD @ 450 nm

Sweep Read Mode: ±1.0% ± 0.010 OD from 0.000 to 1.000 OD @ 405 nm
Repeatability (340 nm to 399 nm)

Repeatability (400 nm to 750 nm)

Sweep Read Mode: ±2.0% ± 0.020 OD from 0.000 to 1.000 OD @ 405 nm
Throughput
96-Well, Dual Wavelength, Normal Read Mode: 73 seconds
96-Well, Single Wavelength, Sweep Read Mode: 18 seconds
Narrow Bean (NB) Models (800TSNB)


Normal and Rapid Read Mode (96-well): 0.000 to 4.000 OD
Normal and Rapid Read Mode (384-well): 0.000 to 4.000 OD

Accuracy
Normal Read Mode (96-well): ±1.0% ±0.010 OD from 0.000 to 2.000 OD @ 405 nm
Rapid Read Mode (96-well) ±2.0% ±0.020 OD from 0.000 to 2.000 OD @ 405 nm
Normal Read Mode (384-well): ±2.0% ±0.020 OD from 0.000 to 2.000 OD @ 405 nm
Rapid Read Mode (384-well): ±2.5% ±0.020 OD from 0.000 to 2.000 OD @ 405 nm
Linearity
Normal Read Mode (96-well): ±1.0% ± 0.010 OD from 0.000 to 2.000 OD at 405 nm
±3.0% ±0.010 OD from 2.000 to 3.000 OD @ 450 nm

Rapid Read Mode (96-well): ±2.0% ± 0.010 OD from 0.000 to 2.000 OD @ 405 nm
Normal Read Mode (384-well): ±2.5% ± 0.010 OD from 0.000 to 2.000 OD @ 405 nm
Rapid Read Model (384-well): ±2.5% ± 0.010 OD from 0.000 to 2.000 OD @ 405 nm
Repeatability (STD)
Throughput
384-Well, Dual Wavelength, Normal Read Mode: 3 minutes, 40 seconds
384-Well, Dual Wavelength, Rapid Read Mode: 2 minutes

Appendix B
Error Codes
This appendix lists and describes 800 TS error codes that may appear in
Gen5.
Error Codes Overview 82

Error Codes 82
82 | Appendix B: Error Codes
Error Codes Overview

When a problem occurs during operation with the 800 TS, an error code may appear on
the touchscreen or in Gen5. Error codes typically contain four characters, such as “4168,”
and in most cases are accompanied by descriptive text. With many errors, the instrument
will beep repeatedly; press the carrier eject button to stop this alarm.
Some problems can be solved easily, whereas others can be solved only by trained BioTek
service personnel. This appendix lists the most common and easily resolved error codes
that you may encounter.
Error codes beginning with “A” (e.g., A100) indicate conditions that require immediate
attention. If this type of code appears, turn the instrument off and on. If the system test
does not conclude successfully, record the error code and contact BioTek’s Technical
Assistance Center.
If an error code appears in Gen5, you should run a system test for diagnostic purposes. In
Gen5, select System > Diagnostics > Run System Test. Having the system test report
before calling the BioTek Technical Assistance Center can speed the resolution of the error.
If an error message appears while an experiment is in process and after

having received measurement data, it is your responsibility to
determine if the data is valid.
Contact Info: BioTek Service/TAC

Use this appendix to diagnose problems and solve them if possible. If you need further
assistance, contact BioTek’s Technical Assistance Center.
Phone: 800-242-4685 (toll free in the U.S.)
802-655-4740 (outside the U.S.)
Fax: 802-654-0638
E-Mail: tac@biotek.com
Error Codes
This table lists the most common and easily resolved error codes that you may encounter.
If an error code appears in Gen5, look for it here. If you find the code, follow the
suggestions provided for solving the problem. If you cannot find the code or if you are
unable to solve the problem, please contact BioTek’s Technical Assistance Center. The
Gen5 Help system also provides troubleshooting tips.

Error Codes | 83
Code Description and Probable Causes

020x Motor sensor not found. Axis fails to home.
x = motor axis
0 = x axis motor
1 = y axis motor
2 = filter motor
Possible causes:
Dirty axis rail where the bearings are worn and cause too much friction.
Defective or broken optical sensors or connection.
Defective motor, motor controller, or connection.
Broken or untensioned drive belt.
Obstruction in read chamber.
Check that the shipping screw has been removed.
Check for motor obstructions.
Run a self-test.
Note: In cases where a sensor is not functioning, the motor may drive the axis to
its mechanical stop and generate substantial noise but will not cause permanent
damage.
040x Motor failed positional verify.
Motor failed to reach the same position when moved a known number of steps
from the home position and back.
x = motor axis
0 = x axis motor
1 = y axis motor
2 = filter motor
Probable causes:
Dirty linear bearing
Obstruction in read chamber
Incorrect belt tension or loose clamp
Troubleshooting:
Restart the instrument (this will reset the home position).


130x Motor not homed successfully.
x = motor axis
0 = x axis motor
1 = y axis motor
2 = filter motor
Check for motor obstructions.
Run a self-test.
05xy Analog reading saturated.
x = filter position
y = readset
Check filter configuration.
Check for light intrusion.
Run self-test.
060x Air reading too low.
x = filter position
Check lamp.
070x Signal to noise too low.
x = filter position
Check for light infiltration.
Ensure reader is not too close to another instrument that generates EMI (hot
plate, freezer, PC monitor)
0800 Analog offset out of range.
Hardware error.
Check for ambient light leaks.
Ensure the power supply is not too close to the instrument.
Call TAC.
0C0x Power supply failure.
x = failed test
Hardware error. Call TAC.

Error Codes | 85

0D0x Attempted read with wavelength that has not been calibrated.
x = readset
Run self-test.
0E0x Attempted read with wavelength that is not present in reader.
x = readset
Run self-test.
0F00 Analog signal error: too low during gain calibration or changed too much
during read.
Check for light infiltation.
Run self-test.
1100 Configuration checksum error. Configuration data corrupted.
This error indicates that the stored checksum value from the configuration data
does not match the actual checksum of the current configuration data.
Recreate the assay definition in the correct version of the software and download
it again.
Call TAC.
1200 Carrier corner calibration mission.
Hardware/software error. Call TAC.

130x Motor not homed successfully.
x = motor axis
0 = x axis motor
1 = y axis motor
2 = filter motor
Check for motor obstruction.
Run self-test.


140x Motor cannot reach expected position.
x = motor axis
0 = x axis motor
1 = y axis motor
2 = filter motor

15xy Incubation error.
x = failure type
y = thermistor bitfield
Run a self-test for more details on failing zone.

1600 Unexpected abort or define assay command checksum failed.
Call TAC.
1700 Invalid kinetic interval.
Check assay.
1900 Memory allocation error.

1A00 Motor position table invalid.

1B00 Analog controller communication error.
Check software versions. Call TAC.

1D00 Filter configuration changed with map off.
Run a self-test.
2100 Invalid command parameter.

2600 Invalid plate dimensions.
Check plate definition.

2700 PC communication error.
Check connection to PC.

Error Codes | 87

280x Motor in use.
x = motor axis
0 = x axis motor
1 = y axis motor
2 = filter motor

2Dxx Assay definition error.
xx = error type

4000 Onboard protocol checksum failed.

4010 Onboard protocol storage full.
Delete protocols to make space.

4020 Onboard protocol duplicate name not allowed.
Change the protocol name to one that is unique.

4030 Onboard protocol name empty.
Change the protocol name.

4040 Onboard protocol blanks defined beyond plate limits.
Verify that the blanks are valid for the plate type.
4050 Absorbance test plate certification past due.
Check test plate certification.


50xx USB errors:
13 = Error writing to USB flash drive
14 = Error reading from USB flash drive
15 = Error creating folder on USB flash drive
16 = Error opening file on USB flash drive
17 = Error closing file on USB flash drive
18 = Error changing folder on USB flash drive
19 = Error writing to USB printer
1A = Error deleting file on USB flash drive
1C = No USB output selected
1D = No USB flash drive detected
Troubleshooting:
Use a different USB flash drive.
Check the printer connection.
Select results output at Instrument > Output.
Insert flash drive.

8000 and up Fatal error. Contact TAC.

Appendix A
Sample Reports
This appendix contains sample System Test and Absorbance Plate Test
reports for the 800 TS.
90 | Appendix C: Sample Reports
Sample System Report

Sample Absorbance Test Plate Report | 91
Sample Absorbance Test Plate Report

Sample Assay Results
Sample (Report) CSV File

Sample Assay Results | 93
Sample (Gen5 input) TXT File


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800 TS Operator's Manual

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On (Supply) Consult instructions for use

800 TS Operator's Manual

BioTek Instruments, Inc.

See Appendix A, Specifications, for performance and technical specifications.

BioTek Instruments, Inc.

7-filter Absorbance Test Plate for absorbance

800 TS Operator's Manual

BioCell adapter plate (holds up to 8 BioCells) 7270512

Materials for Liquid Tests Part #

BioTek Instruments, Inc.

Product Support and Service

Technical Assistance Center (TAC)

800 TS Operator's Manual

BioTek Instruments, Inc.

1. Unpack and Inspect the Reader

BioTek Instruments, Inc.

2. Remove the Shipping Hardware

1. Carefully turn the reader upside down on a level surface.

800 TS Operator's Manual

3. Install the Optional Door in Top Cover

BioTek Instruments, Inc.

4. Verify the Filter Wheel Contents

800 TS Operator's Manual

BioTek Instruments, Inc.