PRACTICAL PHYSIOLOGY

2022-2023

Safety
Assistant lecture
Sleman Y Omar
Sleman.yousif33@gmail.com

Lab 1
 Proper Safety

safety", the condition of being protected from harm or other

danger. To insure safety of those working in the lab,
as well as the integrity of each experiment, each of
the following rules must be followed:

1. Clothing should be protected by a lab coat or

apron.
2. Hair that is long should always be tied back to
avoid contamination as well as safety when
working near the Bunsen burners.
3. Lab stations must be wiped down at the
beginning and at the end of every lab
session. Station cleaning is best
accomplished with fresh 10% bleach. If
there is visible contamination on the bench,
wash with soap and water before the bleach.

4. Avoid direct contact with any microbes

being tested by keeping all cultures well
below mouth, nose, and eye regions. to insure
integrity of cultures, avoid coughing,
excessive talking, laughing, etc, while working
with cultures while open. Keep cultures at a
minimum of exposure to the air for best results.
5. Bunsen burners should be lit from the beginning of
each session to the end as this decreases the risk of
contamination of cultures and helps the safety of the
lab worker.

6. Gloves must ALWAYS be worn when handling any

microbial agent.

7. stations should be kept clear of any extra Lab

materials (non-lab books, book bags, purses, keys,
etc.) to avoid contamination as well as accidents.

8. All lab materials must be stored in the appropriate

locations at the end of each session and gloves and
other disposables placed in biohazard bags.

9. Tubes and racks should be placed in the appropriate

location for autoclaving.
10. Smoking, drinking and eating are prohibited. On no
account should any apparatus or materials used in the
laboratory be placed in the mouth.

11. Microbial cultures should to be opened and

manipulated only near a Bunsen burner with a strong
flame. Under no circumstances should culture material
be touched directly with any part of your body: use
inoculating loops, pipettes etc. to transfer cultures.

12. All instrument that is use in lab must be reported to

the instructor.

13. Always wash and dry your hands before leaving the
laboratory.
14. All spills should be reported IMMEDIATELY to the lab
instructor for proper cleanup. Unreported spills can
result in bio-hazardous conditions.

15. Always wear approved eye protection when staining

or handling hazardous laboratory chemicals and
reagents.

16. Always read the laboratory exercises prior to your

scheduled laboratory meeting.

17. Clearly label all Petri dishes, tubes and flasks. All
culture vessels MUST be labeled with your bench
number and the name of the microbe inoculated when
this is known.
 Instrument Used in practical physiology and
Microbiology Laboratory

The most important equipment which is use in physiology and

Microbiological laboratories:
1. Incubator : Used for incubation of microorganisms culture.
Conditions
 25°C for common fungi for 5-7 day
 37°C for yeasts, bacteria, human pathogenic fungi
2. Autoclave
Is an important equipment in the laboratory of microbiology used for
sterilizing of nutrient media, old culture, distil water, soil at 121°C ,15
pound.
3. Oven: used for sterilization of glassware as tube,
Petri dish, flask, ect…..
4. Hood: used for culture and subculture from old cultures
the hood consist of a chamber made of wood, provided with a
U.V light.
5. Refrigerator: used for preserving microorganism culture
and prepared nutrient media at 4°C the cultures stay a life.

6. Balance: necessary for measuring of solid chemical substances

for nutrient media preparing and solutions.
7. Hot plate: used for melting media for preparing nutrient media.

8. Centrifuge: used for separating of two substances mixed in one

solution or for isolating the microorganism organelles the separation
procedure is depend on the mass and shape of this organelles that
different molecular weight is sediment in different speed.
9. Water bath: used for melting nutrient media and for some
other purpose.

10. Microscope: is an essential instrument used in general biology

and microbiological laboratories for identification of microorganism.
Thank you