SCIENCE LABORATORY MANUAL

Introduction

The aim of this handbook is to enhance the standard safety in school laboratories by drawing the
laboratory users’ attention to the necessary safety precautions to be taken. It also promotes the awareness
of potential hazards to minimize risks and advises on how to effectively deal with any accidents which
may occur.

In recent years, the learning and teaching of science in secondary schools has undergone rapid changes
towards a much greater emphasis on open-minded investigation and experimental approach. It is,
therefore all the more important for science teachers, and students to pay attention to safety precautions
when working in the laboratory or during a field study.

Apart from making sure that the students are in compliance with the safety rules in laboratories, science
teachers should set good examples in this regard, and update themselves with the latest developments in
laboratory safety. The following parts provide rules and regulations inside the science laboratory.

School Core Values

 Excellence
 Character
 Service

Mission

The school ensures that all our students develop knowledge, skills proper behavior and attitude to become
successful individuals and citizens in order to promote excellence in a caring environment and prepare
each student, in a partnership with stakeholders and community, to be a life-long, self-directed learner in
a diverse society.

Vision

Monlimar Development Academy, Inc. is committed to improving learning and producing students with
high academic achievement, balance and well-molded character and willing to serve their country and
their fellowmen.

History

Dr. Lillan S. Roldan Founded Monlimar Development Academy, Inc. on January 16, 2006. The name
comes from the first syllable of the names Lillan and Mario the owners of the school, while “Mon” comes
from the school that was across the street from their house in San Francisco, California. Growing up Mrs.
Roldan was a frustrated teacher, when she was young she would teach her siblings and neighbors using a
small black board that her mother bought for her. Not even in her wildest dreams did she think that one
day she would be the owner of her very own school.
Laboratory Safety Rules

Before entering the laboratory

 Mobile phones must be switched off

 Remove all jewelry
 Laboratory coats and lab shoes must be worn in teaching laboratories
 Long hair should be tied back
 Leave your backpack in the cloak room; take only your lab supplies (Lab & school) for the lesson
and your valuables

Inside the Laboratory

General safety rules

 Don’t eat or drink in the lab

 Know emergency procedures
 Keep your work area and walkways clear of backpacks, books and other obstacles
 Always behave appropriately in the laboratory
 Do not touch any equipment or chemicals in the laboratory until your teacher has given
permission

Accident and injuries

 Instantly report any accident, spill, or injury to your instructor, no matter how minor it is
 Follow all written and verbal instruction carefully
 Know where all of the safety equipment is in the lab and how to use it
 If there is a fire, all electrical and gas equipment must be shut off

When you’re doing an experiment

 Always read the label of a chemical bottle before using it

 Know the potential hazard of a chemical before using the substance
 Label all the glassware containing solutions carefully
 Items of Personal Protective Equipment (PPE) provided for particular procedures must be used
when indicated by the teacher
 Never return unused chemicals to their original containers

At the end of the experiment

 Dispose of chemicals properly (some chemicals can be disposed of in the sink, while others
require to be dispose of in specific containers)
Standard Operating Procedures

A. General Personal Safety

1. Eating, drinking, smoking, applying cosmetics or lip balm, and handling contact lenses
are prohibited in areas where specimens are handled.
2. Food and drink are not stored in refrigerators, freezers, cabinets or on shelves,
countertops, or bench tops where blood or other potentially infectious materials are
stored or in other areas of possible contamination.
3. Long hair, ties, scarves and earrings should be secured.
4. Keep pens and pencils OUT OF YOU MOUTH!!!
5. Appropriate Personal Protective Equipment (PPE) will be used where indicated.

Lab coats or disposal aprons should be worn in the lab to protect you and your clothing
from contamination. Lab coats should NOT be worn outside the laboratory.

Lab footwear should consist of normal closed shoes to protect all areas of the foot from
possible puncture from sharp objects and/or broken glass and from contamination from
corrosive reagents and/or infectious materials.

Gloves should be worn for handling blood and body fluid specimens, touching the mucous
membranes or non-intact skin patients, touching items or surfaces soiled with blood or body
fluid, and for performing venipuncture and other vascular access procedures. Cuts and
abrasions should be kept bandaged in addition to wearing gloves when handling bio
hazardous materials.

6. NEVER MOUTH PIPETTE! Mechanical pipetting devices must be used for pipetting
all liquids.
7. Frequent hand washing is an important safety precaution, which should be practiced
after contact with patients and laboratory specimens.

Proper washing techniques include soap, running water and 10-15 seconds of friction or
scrubbing action. Hands should be dried and the paper towel used to turn the faucets off.

Hands are washed:

A. After completion of work and before leaving the laboratory.

B. After removing gloves.
C. Before eating, drinking, smoking, applying cosmetics, changing contact lenses or using
laboratory facilities.
D. Before all other activities which entail hand contact with mucous membranes.
E. Immediately after accidental skin contact with blood or other potentially infectious
materials.
F. Between patient contact and before invasive procedures.

B. Eye Safety
1. KNOW WHERE THE NEAREST EYE WASH STATIONS IS LOCATED AND
HOW TO OPERATE IT.
 2. Eye goggles should be worn;
A.) When working with certain caustic reagents and/or solvents,
B.) When working with reagents under pressure.
C.) When working in close proximity to ultra-violet radiation (light).
3. Wearing contact lenses in the laboratory is discourages and requires extra precaution if
worn. Gases and vapors can be concentrated under lenses and cause permanent eye
damaged. Furthermore, in the event of a chemical splash into an eye, it is often nearly
impossible to remove the contact lens to irrigate the eye because of involuntary spasm of
the eyelid. Persons who must wear contact lenses should inform their supervisor to
determine which produces would require wearing no-vent goggles.

C. Safe Handling of Biological Hazardous Materials

1. YOU SHOULD HANDLE ALL PATIENT SAMPLES AS POTENTIALLY
BIOHAZARDOUS MATERIAL. This means UNIVERSAL PRECAUTIONS should
be followed at all times!!
2. When Working in the laboratory
A.) Wear protective clothing (lab coat, gloves, and masks). If you have cut /abrasion,
also wear a Band-Aid.
B.) Avoid spillage and aerosol formation.
C.) Hands should be washed immediately and thoroughly if contaminated with blood
or other body fluids.
D.) Gloves should be removed before handling a telephone, computer, keyboard, etc.,
and must NOT be worn outside the immediate work area. Hands should always be
washed immediately after gloves are removed.
E.) You should wash your hands after completing laboratory activities and before
leaving the area. All protective clothing should be removed prior to leaving the
lab.
F.) All bio hazardous material should be discarded in a biohazard bag to be
autoclaved.
G.) All counter and table tops should be disinfected with a proper disinfecting
solution.
a) At the beginning of the day.
b) If you should spill a patient sample.
c) At the end of the day.
3. Proper handling of SHARPS:
A.) Contaminated needles and other sharps are never broken, bent, recapped or re-
sheathed by hand.
B.) Used needles are not removed from disposable syringes.
C.) Needles and sharps are disposed of in impervious containers located near the
point of use.
LIST OF AVAILABLE EQUIPMENTS/APPARATUS

LAB EQUIPMENT/S DESCRIPTION/USAGE QUANTITY

Alcohol Lamp Used as source of heat or light 8
Beaker A deep, wide mouthed, thin walled, 1000 ml (1)
cylindrical vessel with a pouring lip used 400ml (8)
for measuring large quantity of liquid, also 250 ml (2)
a container in boiling liquid mixture. 150 ml (2)
100 ml (2)
50 ml (2)
Cover Slip A small square glass that is used to cover 10 boxes
the samples in glass slide.
Crucible and Cover A vessel for heating or melting substances
7
requiring extreme heat and temperature.
Crucible Tong Used in holding hot materials or apparatus. 5
Denatured Alcohol Use as a solvent and as a fuel for alcohol 1L
burners.
Dissecting Kit A complete set of equipment used in
7
dissection.
Dissecting Pan An aluminum pan with paraffin wax used to
7
attach the specimen during dissection.
Erlenmeyer Flask Used for measuring large quantity of liquid, 500 ml (9)
used as receiver in titration and distillation; 250 ml (11)
used also as a container for liquid samples. 150 ml (1)
100 ml (3)
50 ml (1)
Evaporating Dish A porcelain dish in which a solution maybe
evaporated to leave the dissolve substance 7
as a residue.
Florence Flask Used as a container for boiling liquid
solution, used for measuring large quantity 250 ml (1)
liquid.
Funnel A cone-shaped vessel ending in a tube at
the base, used for pouring liquids or
8
powders through a small opening and to
hold the filter paper in filtration.
Glass Dropper A tube for measuring or administering L (7)
liquid by drop. S (7)
T (14)
Goggles It is a safety glasses, are forms of protective
eyewear that usually enclose or protect the
4
area surrounding the eye in order to prevent
particulates, water or chemicals form.
Graduated Cylinder Used for measuring volume of liquid. 250 ml (3)
100 ml (11)
50 ml (7)
25 ml (4)
10 ml (4)
Inoculating Loop A simple tool used mainly to retrieve an
7
inoculum from a culture of micro-organism.
Iron Ring & Clamp It is used to hold the wire gauze. 3
Iron Stand It is used to support the iron ring and clamp. 3
Litmus paper To test whether a solution is acidic or basic. Blue (1)
Red (1)
Microscope An optical instrument used to examine
minute objects by giving an enlarged, well 7
resolved image of them.
Microscope Slide A thin flat piece of glass, typically 75by 26
mm (3 by inches) and about 1 mm thick,
2 boxes
used to hold objects for examination under
a microscope.
Mortal Use for pounding and grinding solid
7
substances into powder form.
Methylene Blue A liquid used to stain specimen. 1 bottle
Pestle Used for pounding and grinding solid
8
substances into powder form.
Plastic Dropper A tube for measuring or administering
10
liquid by drop.
Petri Dish A shallow glass or plastic cylindrical lidded
dish that biologist use to culture cells or 7
small moss plant.
Pipet Used for measuring small volume of liquid
3
direct from the reagent bottle.
Spatula An instrument with a broad, flexible,
usually dull-edged blade, used for mixing,
2
scooping or spreading soft substances and
liquids.
Syringe A tube with a nozzle and piston or bulb for
sucking in and ejecting liquid in a thin
Glass (1)
stream, used for cleaning wounds or body
Plastic (12)
cavities, or fitted with a hallow needle for
injecting or withdrawing fluids.
Stirring rod A slender glass shaft used for stirring and as
7
guide.
Test Tube Glass tubes, closed at one end used in XS (6)
making chemical tests; used for heating S (16)
small amount of solution in experiments. M (12)
L ( 9)
XL (10)
Test Tube Brush Used for cleaning test tubes. 10
Test Tube Holder A metal wire device used to hold test tubes. 8
Test Tube Rack A framework wooden or metal bar used for
supporting the test tubes when not in use or
7
while waiting for the reaction of reagents to
take place.
Thermometer Used for measuring temperature Glass (4)
Tripod A stand or support with three legs that is
often used to place above the Bunsen
7
burner or alcohol lamp to heat/ boil
anything.
Tweezers A small tools used for picking up objects
too small to be easily handled with the 7
human fingers.
Vials Used as container in storing small amount Glass (1)
of solution or reagents. Plastic (12)
Volumetric Flask Used for measuring large quantity of
7
Volatile liquid.
Watch Glass A crystal used for observing small amount
7
of sample undergoing reaction.
Wire Gauze Fine wire woven to make an open gauzy
7
material for strainers; used to regulate.

SCHEDULE OF LABORATORY

DAY TIME GRADE TEACHER

Monday NO SCHEDULES
7:00 – 7:50 AM 7 – Orion
Tuesday 7:50 – 8:40 AM 7 – Aquarius Ms. Coleen Ida Quiñones
8:55 – 9:45 AM 7 - Scorpios
7:00 – 7:50 AM 8 – Heather
Wednesday 7:50 – 8:40 AM 8 – Anthorium Ms. Kaye Ann M. Damian
11:45 – 12:55 PM 8 – Aster
9:45 – 10:35 AM 9 – Diamond
Thursday 10:35 – 11:30 AM 9 – Alexandrite Ms. Coleen Ida Quiñones
12:05 – 12:55 PM 9 - Sapphire
8:40 – 9:30 AM 10 – Ganymede
Friday 9:45 – 10:35 AM 10 – Europa Ms. Kaye Ann M. Damian
10:35 – 11:25 AM 10 - Callisto

CALENDAR OF ACTIVITIES

MONTH LIST OF LABORATORY ACTIVITIES

June Science Laboratory Orientation
Rules and Regulations inside the Laboratory and Proper Attire.
July Dissolving Substances
Against All Odds
Lung Model
Inside the “Third rock from the Sun”
August Loving Lab Tools
In Other Words
Similar BUT NOT Identical
Modeling the Lithosphere
September Miniature World of Plants and Animals
Finding Faults
Is it Salt or Sugar?
Safe under the Sun
October Microbes Galore
PAGASA Weather Maps
 Properties of Ionic Series 7 Parallel Circuit
November Fastest Airplane
Separating Mixtures
SCI-MATH CELEBRATION
Properties of Covalent
December What a WAVE!
Inside the Atom
Magma Viscosity
January Domino Wave
Natural Power Purifier: A Gift of Nature
Do it yourself clean water
My very own DNA
February Where in the World
My Ecological Footprint
Conserving Momentum
Who is my Daddy?
March Hiding a Giant
Circle of Life
Shake, Rub and Roll
Survival of the Fittest
 CALENDAR OF ACTIVITIES

GRADE 7 LEARNING ACTIVITIES 2019-2020

No. of
Quarter Month Activities/ experiments
Hours
 Science Laboratory Orientation
JUNE  Rules and Regulations inside the Laboratory and 1
Proper Attire.
 Dissolving Substances
 Against All Odds
JULY 1
1ST QUARTER  Lung Model
 Inside the “Third rock from the Sun”
 Loving Lab Tools
 In Other Words
AUGUST 1
 Similar BUT NOT Identical
 Modeling the Lithosphere
 Miniature World of Plants and Animals
 Finding Faults
SEPTEMBER 1
 Is it Salt or Sugar?
 Safe under the Sun
 Microbes Galore
2ND QUARTER OCTOBER  PAGASA Weather Maps 1
 Properties of Ionic Series 7 Parallel Circuit
 Fastest Airplane
 Separating Mixtures
NOVEMBER 1
 SCI-MATH CELEBRATION
 Properties of Covalent
 What a WAVE!
DECEMBER  Inside the Atom 1
 Magma Viscosity
3RD QUARTER  Domino Wave
 Natural Power Purifier: A Gift of Nature
JANUARY 1
 Do it yourself clean water
 My very own DNA
 Where in the World
 My Ecological Footprint
FEBRUARY 1
 Conserving Momentum
 Who is my Daddy?
4TH QUARTER
 Hiding a Giant
 Circle of Life
MARCH 1
 Shake, Rub and Roll
 Survival of the Fittest
 CALENDAR OF ACTIVITIES

GRADE 7 HOME-BASED LEARNING ACTIVITIES 2020-2021

No. of
Quarter Month Activities/ experiments
Hours
 Science Laboratory Orientation
JULY  Rules and Regulations inside the Laboratory 1
and Proper Attire.
 Creating Mixture
1ST QUARTER AUGUST 1
 Separating components of Mixture
 Food Mixture Party
SEPTEMBER  Preserving Fruits Using Different Types of 1
Sugar Syrup
 Using Microscope
OCTOBER 1
 Creating Miniature Microscope Model
2ND QUARTER NOVEMBER  Protists in a Jar 1
 Creating Brochure of plant cell and animal
DECEMBER 1
cell
 Average Speed
JANUARY 1
 Velocity and Acceleration
3RD
QUARTER
 Heat Transfer
FEBRUARY 1
 Transmission of Sound Waves
 Recycling
MARCH  Philippine Environmental Issues 1
4TH QUARTER  SCI-MATH CELEBRATION
 The Layers of the Atmosphere
APRIL 1
 Weather Observations
 CALENDAR OF ACTIVITIES

GRADE 7 LEARNING ACTIVITIES 2021-2022

No. of
Quarter Month Activities/ experiments
Hours
 Science Laboratory Orientation
AUGUST  Rules and Regulations inside the Laboratory and 1
Proper Attire.
 Creating Mixture
SEPTEMBER 1
1 QUARTER
ST
 Separating components of Mixture
 Food Mixture Party
 Preserving Fruits Using Different Types of Sugar
OCTOBER 1
Syrup
 SCI-MATH CELEBRATION
 Using Microscope
NOVEMBER 1
 Creating Miniature Microscope Model
2ND
QUARTER
 Protists in a Jar
DECEMBER 1
 Creating Brochure of plant cell and animal cell
 Average Speed
JANUARY 1
 Velocity and Acceleration
3RD QUARTER
 Heat Transfer
FEBRUARY 1
 Transmission of Sound Waves
 Recycling
MARCH 1
 Philippine Environmental Issues
4TH QUARTER
 The Layers of the Atmosphere
APRIL 1
 Weather Observations
 CALENDAR OF ACTIVITIES

GRADE 8 LEARNING ACTIVITIES 2019-2020

No. of
Quarter Month Activities/ experiments
Hours
 Science Laboratory Orientation
JUNE  Rules and Regulations inside the Laboratory and 1
Proper Attire.
 Dissolving Substances
 Against All Odds
JULY 1
1ST QUARTER  Lung Model
 Inside the “Third rock from the Sun”
 Loving Lab Tools
 In Other Words
AUGUST 1
 Similar BUT NOT Identical
 Modeling the Lithosphere
 Miniature World of Plants and Animals
 Finding Faults
SEPTEMBER 1
 Is it Salt or Sugar?
 Safe under the Sun
 Microbes Galore
2ND QUARTER OCTOBER  PAGASA Weather Maps 1
 Properties of Ionic Series 7 Parallel Circuit
 Fastest Airplane
 Separating Mixtures
NOVEMBER 1
 SCI-MATH CELEBRATION
 Properties of Covalent
 What a WAVE!
DECEMBER  Inside the Atom 1
 Magma Viscosity
3RD QUARTER  Domino Wave
 Natural Power Purifier: A Gift of Nature
JANUARY 1
 Do it yourself clean water
 My very own DNA
 Where in the World
 My Ecological Footprint
FEBRUARY 1
 Conserving Momentum
 Who is my Daddy?
4TH QUARTER
 Hiding a Giant
 Circle of Life
MARCH 1
 Shake, Rub and Roll
 Survival of the Fittest
 CALENDAR OF ACTIVITIES

GRADE 8 HOME-BASED LEARNING ACTIVITIES 2020-2021

No. of
Quarter Month Activities/ experiments
Hours
 Science Laboratory Orientation
JULY  Rules and Regulations inside the Laboratory and 1
Proper Attire.
1ST QUARTER  Newton’s Second Law of Acceleration
AUGUST 1
 Friction
 Power
SEPTEMBER 1
 A Fruit Cell
OCTOBER  Tracking Typhoons 1
2ND
QUARTER NOVEMBER  Observing Minor Members of the Solar System 1
DECEMBER  A Comet Model 1
 Particle Model of Matter
JANUARY 1
 Three States of Matter
3 RD
QUARTER
 Surface Tension
FEBRUARY 1
 Atomic Model
 To Chew or Not to Chew
MARCH  My Diet 1
4TH QUARTER  SCI-MATH CELEBRATION
 Heredity or Environment
APRIL 1
 Biodiversity
 CALENDAR OF ACTIVITIES

GRADE 8 HOME-BASED LEARNING ACTIVITIES 2021-2022

No. of
Quarter Month Activities/ experiments
Hours
 Science Laboratory Orientation
AUGUST  Rules and Regulations inside the Laboratory and 1
Proper Attire.
 Science Laboratory Orientation
1 QUARTER
ST
SEPTEMBER  Rules and Regulations inside the Laboratory and 1
Proper Attire.
 Newton’s Second Law of Acceleration
OCTOBER  Friction 1
 SCI-MATH CELEBRATION
 Power
NOVEMBER 1
2ND QUARTER  A Fruit Cell
DECEMBER  Tracking Typhoons 1
JANUARY  Observing Minor Members of the Solar System 1
3RD QUARTER
FEBRUARY  A Comet Model 1
 Particle Model of Matter
MARCH 1
 Three States of Matter
4TH QUARTER
 Surface Tension
APRIL 1
 Atomic Model