Republic of the Philippines

Department of Education
Region IX, Zamboanga Peninsula
Division of Zamboanga del Sur
TUKURAN TECHNICAL-VOCATIONAL HIGH SCHOOL
Tukuran, Zamboanga del Sur

Teacher: HARRIS A. ABDULGANI Date: December 10, 2019

Grade: 10 Section: Oriole

I. OBJECTIVES: At the end of the lesson, the students will be able to:
a. Identify the process involve in DNA Replication.
b. Create a model of DNA Replication.
c. Appreciate the importance of DNA Replication.

II. SUBJECT MATTER

a. Topic: DNA Replication
b. Reference: Science 10 Learner’s Material; Marlene B. Ferido; pp. 268-272
c. Materials: Power Point Presentation, Laptop, Group Activity Materials
d. Strategy/Methods Used: ICT Integration, Inquiry-Based Instruction, Differentiated Activities, Collaborative Learning and
Student-Centred Learning

III. LEARNING PROCEDURES

A. Preliminary Activities (Indicator 5)
 Prayer
 Greetings
 Checking of Attendance
 Classroom Management

1. ELICIT
 The teacher will ask the students about their previous topic.

2. ENGAGE (Indicator 3 and 4)

Activity 1: You’re My Perfect Match
 The teacher will call a students to match the following images with its exact replica.
 The students will choose from the pictures inside the box.

Images Replica

 The teacher will ask the following questions to the

students:
1. What have you observe from our activity? (Students answer may vary.)
 2. What do you think is our topic for today? (Students answer may vary.)
 The teacher will let the students read the objectives.

3. EXPLORE (Indicator 1, 6 and 8)

 The teacher will group the students into four (4). (Across Values Education)
(Students will pick a paper from the box to determine what group they belong.)
 Group Indicator: 4 Core Values: (Makadiyos, Makatao, Makakalikasan at Makabansa)

Activity 2: DNA Makes DNA

Objective: • Make a model of a DNA template to determine the sequence of bases in the new DNA strand.

Materials: • color paper • scissors • paste/tape • 1/4 manila paper

Procedure:

1. Use the patterns of the components of the DNA provided by your teacher. Color code phosphate = blue, deoxyribose sugar = green
and nitrogenous bases as follows: adenine = yellow, thymine = pink, guanine = violet and cytosine = red.

2. Cut out the shapes of each nucleotide.

3. Build a model of a strand of a DNA molecule. The strand should contain 6 base “rungs” following the given order of the nucleotides
below. Guanine Adenine Cytosine Thymine Cytosine Guanine.

4. Tape the cut out pattern to form the nucleotides.

5. Let this arrangement represent the left half of your DNA molecule.

6. Make a complementary strand for the first strand that you made in step 3.

7. Tape the cut-out pattern forming nucleotides for the second strand of the DNA molecule.

8. Match the bases of the first strand and the second strand. Do not tape across bases.

9. Once you have made your DNA model, separate the two strands of the DNA model down the middle so that there are now two
single strands of DNA.

10. Create new double-stranded DNA by matching complementary nucleotides to the bases on each single strand.

11. Tape and then cut out the pattern forming the nucleotides for each of the single nucleotides.

12. When you are finished, mount the original DNA model and the DNA model with its complementary strand in the illustration board
or folder.

Guide Questions:

Q1. Compare the two new strands of DNA. Are they the same or different? Why?

Q2. How do the nucleotides in DNA pair?

Q3. How do you compare a DNA molecule to a zipper?

Q4. How is information from the DNA passed on from one cell to another?

4. EXPLAIN (Indicator 2)
Each group will explain their outputs in front of the class.
5. ELABORATE (Indicator 8)
The teacher will give further information about DNA Replication using the power point presentation.

The following are the events while DNA copies itself:

• Step 1. An enzyme called helicase breaks the bond between nitrogenous bases. The two strands of DNA split.
• Step 2. The bases attached to each strand then pair up with the free nucleotides found in the cytoplasm.
• Step 3. The complementary nucleotides are added to each strand by DNA polymerase to form new strands. Two new DNA
molecules, each with a parent strand and each with a new strand are formed. The DNA replication is known as semi-conservative
replication, because one of the old strands is conserved in each new molecule. Figure 4 illustrates the semi-conservative
replication of DNA.
  Why is DNA Replication necessary to life?
 DNA replication is important because without it, cell division could not occur. ... With DNA replication, the set of
DNA of a cell can be duplicated and then each cell that results from division can have its own entire set of DNA
and cell division can theoretically continue indefinitely.

6. EVALUATION (Indicator 9)
Multiple Choices
Direction: Write only the letter of the correct answer.
1. What enzyme, pictured above, breaks apart the hydrogen bonds between two strands of DNA?
a. Histone c. Exonuclease
b. Helicase d. Endonuclease
2. What is the mechanism of DNA Replication?
a. Semi-conservative c. Complementary
b. Conservative d. Dispersive
3. Which of the following complementary base is correct?
a. A=G c. A=T
b. G=T d, T=U
4. Which of the following steps is the correct sequence of DNA Replication?
a. Unwinding and strand separation, Adding complementary bases and Formation of Different DNA molecules
b. Unwinding and strand separation, Adding complementary bases and Formation of two identical DNA
molecules
c. Unwinding and strand separation, Formation of Different DNA molecules and Adding complementary bases
d. Unwinding and strand separation, Formation of two identical DNA molecules and Adding complementary
bases
5. Why is DNA Replication important?
a. It is the blueprint of our life.
b. It maintains homeostasis in our body.
c. It is required for a cell division to happen.
d. It blocks foreign bodies away from the cell.
7. EXTEND
In your science notebook, give a brief description about the functions of the following enzymes in DNA Replication:
1. DNA Helicase
2. DNA Polymerase
3. Primase
4. Ligase
5. Gyrase

IV. Remarks

V. Reflections

a. No. of learners who earned 80% in the evaluation

b. No. of learners who required additional activities for remediation.
c. Did the remedial lesson work? No. of learners who have caught up with the lesson
d. No. of learners who continue to require remediation
e. Which of my teaching strategies worked well? Why these work?
f. What difficulties did I encounter which my principal or supervisor can help me solve?
g. What innovation or localized materials did I use/discover which I wish to share with other teachers?
Checked by: Noted by:

MYLA C. MARCOS LUTHER D. CASTELO, Ed.D.

Science Department Head School Principal