KING HENRY VIII CHEMISTRY DEPARTMENT – REQUIRED PRACTICAL 1

NAME : DATE:

Required practical Make up a volumetric solution and carry out a simple

acid-base titration
Apparatus and e. Use volumetric flask, including accurate technique for
techniques covered making up a standard solution
k. Safely and carefully handle solids and liquids, including
(Relevant apparatus only, corrosive, irritant, flammable and toxic substances
not full statements)
Indicative apparatus Basic laboratory glassware, volumetric flask, burette,
volumetric pipette and filler, and protective equipment such as
goggles.
Opportunities for observation and assessment level of key competencies
Green ☐
1: Follows written instructions Orange ☐
Red ☐
a) Students follow written method which allows them to complete the
practical task safely and successfully.

4: Makes and records observations Green ☐

Orange ☐
b) Students should obtain accurate, precise and sufficient data for Red ☐
experimental and investigative procedure.
Students should record all data methodically using appropriate
units and conventions.

FEEDBACK
 KING HENRY VIII CHEMISTRY DEPARTMENT – REQUIRED PRACTICAL 1

PART A

To prepare a solution of sodium hydrogensulfate that has a known concentration.

Requirements

 weighing bottle or boat

3
 one 250 cm volumetric (graduated) flask
 sodium hydrogensulfate solid (see below)
 filter funnel
 spatula
 de-ionised or distilled water in a wash bottle
3
 one 250 cm beaker
 glass rod
 digital mass balance (reading to 2 or 3 decimal places)

The composition of the sodium hydrogensulfate should be known; either anhydrous (and the
purest available) or the monohydrate. Students need to be advised which they are using.
Suppliers can also call this reagent sodium bisulphate.

Risk Assessment

SUBSTANCE/APPARATUS HAZARD ACTION

Sodium hydrogen sulfate Corrosive Goggles, gloves

Wash with cold water in case of
spillage
Glassware Breakage Notify teacher
Sweep up pieces using dustpan
and brush
 KING HENRY VIII CHEMISTRY DEPARTMENT – REQUIRED PRACTICAL 1

PART A

TASK: To prepare 250 cm3 of a solution of sodium hydrogensulfate with a known

concentration in the range 0.0900 to 0.110 mol dm–3

Method

a) Calculate the mass of sodium hydrogensulfate solid needed to produce 250 cm3 of a
0.100 mol dm–3 solution. Show your working. If you are using the anhydrous solid, the
mass to weigh out will be between 2.7 and 3.3 g, and if you are using the monohydrate,
the mass to weigh out should be between 3.1 and 3.8 g (A)

b) Weigh a clean dry weighing bottle (or weighing boat).

c) Place the weighing bottle on the pan of a digital balance and, using a spatula, place
into the bottle approximately the mass of sodium hydrogensulfate that you have
calculated to be necessary.

d) Weigh the weighing bottle and its contents accurately and record the precise mass.

e) Pour the contents of the weighing bottle into a beaker and re- weigh the weighing
bottle (which may still contain traces of sodium hydrogensulfate).

f) Calculate the mass of sodium hydrogensulfate that you have transferred. Remember to
record all weighings to the precision of the balance that you have used. (B)

g) Add approximately 100 cm3 of de-ionised (or distilled) water to the beaker containing the
solid and using a glass rod, stir the contents of the beaker until all of the sodium
hydrogensulfate dissolves.

h) Using a funnel, pour the contents of the beaker into a 250 cm3 volumetric (graduated)
flask and then using the wash bottle rinse the beaker and funnel into the same volumetric
flask. Rinse the glass rod into these washings.

i) Make the volumetric flask up to the graduated mark by carefully adding de-ionised water
from the wash bottle. You will need to be careful so that you do not over-shoot the mark.

j) Stopper the volumetric flask and shake it thoroughly to mix the contents of the flask.

k) Calculate the exact concentration in mol dm–3 of your solution quoting the value to the
appropriate precision. Show all of your working. (C)
 KING HENRY VIII CHEMISTRY DEPARTMENT – REQUIRED PRACTICAL 1

Calculation and recording sheet

(A)

Calculate No moles of sodium hydrogen sulphate in 250cm3 of 0.100moldm-3 solution.

Moles = concentration x volume
Moles = 0.10 x (250.00/1000.00)
Moles = 0.025 moles (2 d.p.)

Calculate Mr of sodium hydrogen sulphate

Sodium hydrogen sulphate = NaHSO4
Mr (NaHSO4) = 23.00 + 1.00 + 32.10 + (16.00 x 4.00)
= 120.10 (2 d.p.)

Calculate the mass required

Mass = moles x Mr
Mass = 0.025 x 120.10
Mass = 3.00g (2 d.p.)

(B)

Construct an appropriate weighing table

WHAT IS BEING WEIGHED READING ON SCALE (g)

Mass of weighing boat 0.97
Mass of weighing boat and substance 3.97
Mass of weighing boat after transfer 0.97
Mass of substance transferred 3.00

(C)

Using the actual mass dissolved into the volumetric flask, calculate the number of moles in
250cm3 volumetric flask.
Moles = mass/Mr
Moles = 3.00/120.10
Moles = 0.025 moles (2 d.p.)

Calculate the exact concentration of your solution.

Volume = 250.00 cm3 = (250.00/1000.00) dm3 = 0.25dm3

Concentration = moles/volume
Concentration = 0.025/0.25
Concentration = 0.10 moldm-3

My solution has a concentration of …………………………0.10……………………….moldm-3

 KING HENRY VIII CHEMISTRY DEPARTMENT – REQUIRED PRACTICAL 1

PART B

To determine the concentration of a solution of sodium hydroxide by titration using a

sodium hydrogensulfate solution that has a known concentration.

Requirements

 burette
 stand and clamp
 25 cm3 pipette
 pipette filler
 Two 250 cm3 conical flasks
 Two 250 cm3 beakers
 funnel
 wash bottle
 phenolphthalein indicator
 standard sodium hydrogensulfate solution (150 cm3)
 sodium hydroxide solution (150 cm3)

The sodium hydrogensulfate solution needs to be a solution with an accurately known

concentration between 0.0900 and 0.100 mol dm-3 and should be the solution which you
prepared from Work Sheet 1a.

Risk Assessment

SUBSTANCE/APPARATUS HAZARD ACTION

Sodium hydrogen sulphate Corrosive Goggles,

Wash with cold water in case of
spillage
Sodium Hydroxide Irritant Goggles,
Wash with cold water in case of
spillage
Phenolphthalein indicator Toxic Goggles
Avoid ingestion
Glassware Breakage Notify teacher
Sweep up using dustpan and
brush.
 KING HENRY VIII CHEMISTRY DEPARTMENT – REQUIRED PRACTICAL 1

PART B

TASK: To determine the concentration of a solution of sodium hydroxide by titration

using a sodium hydrogensulfate solution that has a known concentration.

Method

a) Pour approximately 100 cm3 of the sodium hydrogensulfate solution into a clean, dry
beaker that is labelled ‘sodium hydrogensulfate’. Use a small volume of this solution to
rinse the burette before filling it with the sodium hydrogensulfate solution.

b) Pour approximately 100 cm3 of the sodium hydroxide solution into a second clean, dry
beaker labelled ‘sodium hydroxide’.

c) Rinse a 25 cm3 pipette with the sodium hydroxide solution provided and then, using a
pipette filler, pipette exactly 25.0 cm3 of sodium hydroxide solution into a 250 cm3
conical flask (which has been rinsed with de-ionised water).

d) Add two to three drops of phenolphthalein indicator to the solution in the conical flask
and note the colour of the indicator in alkali.

e) Before you start to titrate, construct a Table ready to record your results. (D)

f) Record the initial burette reading. Make sure that all your burette readings are to the
appropriate precision.

g) Titrate the contents of the conical flask by adding sodium hydrogensulfate solution to it
from the burette. Add the sodium hydrogensulfate solution slowly, swirling the flask
gently to mix the solution. Add the sodium hydrogensulfate solution dropwise near the
end-point until the indicator undergoes a definite colour change; this is the end-point of
the titration. Record the colour change in your results. Record the final burette reading in
your Table of results.

h) Calculate and record in your Table of results the volume of sodium hydrogensulfate
solution used.

i) Repeat the titration until you obtain two results, which are concordant. You should
normally carry out at least three titrations. Record all of the results that you obtain.

j) Calculate and record the mean volume of sodium hydrogensulfate solution used in the
titration. Show your working.

k) Determine the concentration of the sodium hydroxide solution used. (E)

 KING HENRY VIII CHEMISTRY DEPARTMENT – REQUIRED PRACTICAL 1

Calculation and recording sheet

(D)
Titration table

TITRATION ROUGH 1 2 3
NUMBER

INITITIAL 0.00 0.10 0.00 0.10

BURETTE
READING (cm3)
FINAL 32.00 32.90 33.10 33.10
BURETTE
READING (cm3)
TITRE 32.00 32.80 33.10 33.00
(cm3)
√ √

Tick the concordant results

Calculate your average titre from your results (√)

Average titre = (33.10+33.00)/2

Average titre = 33.05 cm3 (2 d.p.)

My average titre is ……33.05……….cm3

(E)

Write the equation of the reaction between sodium hydrogen sulphate and sodium
hydroxide.
NaHSO4 (aq) + NaOH(aq) → Na2SO4 (aq) + H2O(l).

Using your average titre calculate the number of moles in 25cm3 of sodium hydroxide
solution.
n(NaHSO4) = concentration x volume = 0.10 x (33.05/1000)
n(NaHSO4) = 3.305x10-3
molar ratio NaHSO4: NaOH = 1 : 1
Therefore, moles of NaOH in 25cm3 = 3.305x10-3

Calculate the exact concentration of the sodium hydroxide solution

Moles in 25cm3 = 3.305x10-3
Moles in 100cm3 = 3.305x10-3 x 4 = 0.01322

Concentration = moles/volume
Concentration = 0.01322/100
Concentration = 1.32x10-4 moldm-3 (2 d.p.)

The exact concentration of the sodium hydroxide solution is ………………1.32x10-4….…. mol dm-3