HISTO 30 POINTS

1. What is infiltration? 19. Light microscopy

Infiltration is the process of completely removing the clearing agent from the Plastic (Resin) Embedding
tissue and replaced by a medium that will completely fill all the tissue cavities 20. Size (mm) wax surround the tissue
and interstitial tissues. At least 2-4 mm
2. Melting point of paraffin wax 21. Determines the number of rotation made by the wheel
55-60°C Ratchet feed wheel
3. (3-5) Methods of Paraffin wax impregnation 22. 2 ways of deparaffinization (?)
Manual Processing Removal of excess wax by the use of alcohol lamp
Automatic Processing Dipping several times in xylene
Vacuum Processing Placed in oven at 60 degrees Celsius for 15-30 mins
6. Melting point if lab temp is between 15-18°C 23. 3 types of microtome
50-54°C Rocking Microtome
7. Volume of impregnating medium should be at least _____ the volume of the tissue? Rotary Microtome
25 times Sliding Microtome
8. The paraffin oven must be maintained at a temperature of (daghan choices pa)
2-5 degrees Celsius above the melting point 24. Knives (?)
9. More resilient than paraffin wax (?) Plane Wedge
Paraplast Plane Concave
10. Semi-synthetic wax recommended for embedding eyes (?) Biconcave
Bioloid Tool edge or D profile
11. A polyethylene glycol containing 18 or more carbon atoms
Carbowax
12. Other name for Celloidin
Colloidin
13. Purified form of nitrocellulose (?)
Celloidin or Celloidin Impregnation
14. Rarely used EXCEPT when dehydration is to be avoided
Gelatin Impregnation
15. Another name for embedding
Casting or Blocking
16. (16-18) 3 types of disposable embedding bears?
Peel-Away
Plastic Ice Trays
Paper Boats