0.0524 0.2186 0.3579 0.

4864
0.0062 0.207 0.3111 0.4103
0.0884 0.175 0.2453 0.2886
0.097 0.238 0.2356 0.2309
0.1131 0.1571 0.1968 0.2277
0.1015 0.1666 0.198 0.2281

Absorbance (nm) vs time (min) at different enzyme concentrations

fold 0 5 10 15 dead (NaOH)
5.1 0.0524 0.2186 0.3579 0.4864 3.4138 5 Fold (
5.2 0.0062 0.207 0.3111 0.4103 2.7532 0.6
10.1 0.0884 0.175 0.2453 0.2886 1.9447
0.5
10.2 0.097 0.238 0.2356 0.2309 1.587 f(x) = 0.028826 x + 0.06263

Absorbance (401nm)
0.4 R² = 0.996466820214703
20.1 0.1131 0.1571 0.1968 0.2277 1.0143
20.2 0.1015 0.1666 0.198 0.2281 0.8928 0.3

0.2

0.1

0
0 2 4 6
Time (

10 Fold (1
0.35

0.3
f(x) = 0.013418 x + 0.09869
Absorbance (401nm)

0.25 R² = 0.979353725529446
0.2

0.15

0.1

0.05

0
0 2 4 6 8
Time (m

20 Fold (
0.25

f(x) = 0.00767 x + 0.11615

0.2 R² = 0.994063557046594
Absorbance (401nm)

0.15

0.1

0.05
 f(x) = 0.00767 x + 0.11615
0.2 R² = 0.994063557046594

Absorbance (401nm)
0.15

0.1

0.05

0
0 2 4 6
Time (

5 fold -1
0.6 0.45
0.4 f(x) = 0.13164 x
0.5 R² = 0.9665132
f(x) = 0.14413 x − 0.0815 0.35
R² = 0.996466820214703
0.4 0.3
0.25
0.3
0.2
0.2 0.15
0.1
0.1
0.05
0 0
0.5 1 1.5 2 2.5 3 3.5 4 4.5 0.5 1 1.5

10 fold - 1
0.35 0.25
f(x) = 0.0399
R² = 0.55847
0.3
f(x) = 0.06709 x + 0.0316 0.2
R² = 0.979353725529446
0.25

0.2 0.15

0.15
0.1
0.1
0.05
0.05

0 0
0.5 1 1.5 2 2.5 3 3.5 4 4.5 0.5 1 1.5
 20 fold - 1
0.25 0.25
f(x) = 0.03835 x + 0.0778 f(x) = 0.04112 x +
0.2 R² = 0.994063557046594 R² = 0.959294497
0.2

0.15 0.15

0.1 0.1

0.05 0.05

0 0
0.5 1 1.5 2 2.5 3 3.5 4 4.5 0.5 1 1.5
 5 Fold (1) 5 Fold (2)
0.45
0.4 f(x) = 0.026328 x + 0.03619
28826 x + 0.06263 R² = 0.966513247169886
0.35

Absorbance (401nm)
6466820214703
0.3
0.25
0.2
0.15
0.1
0.05
4 6 8 10 12 14 16 0
0 2 4 6 8 10 12 14
Time (min) Time (min)

10 Fold (1) 10 Fold (2)

0.25
f(x) = 0.007986 x + 0.14048
R² = 0.558475916062841
013418 x + 0.09869 0.2
79353725529446
Absorbance (401nm)

0.15

0.1

0.05

4 6 8 10 12 14 16 0
0 2 4 6 8 10 12 14
Time (min) Time (min)

20 Fold (1) 20 Fold (2)

0.25

.00767 x + 0.11615 f(x) = 0.008224 x + 0.11187

994063557046594 0.2 R² = 0.959294497566666
Absorbance (401nm)

0.15

0.1

0.05
 .00767 x + 0.11615 f(x) = 0.008224 x + 0.11187
994063557046594 0.2 R² = 0.959294497566666

Absorbance (401nm)
0.15

0.1

0.05

0
2 4 6 8 10 12 14 16 0 2 4 6 8 10 12 14
Time (min) Time (min)

5 fold -2

f(x) = 0.13164 x − 0.0954500000000001

R² = 0.966513247169886

0.5 1 1.5 2 2.5 3 3.5 4 4.5

10 fold - 2
.25
f(x) = 0.03993 x + 0.10055
R² = 0.558475916062841
0.2

.15

0.1

.05

0
0.5 1 1.5 2 2.5 3 3.5 4 4.5
 Chart Title

f(x) = 0.04112 x + 0.07075

R² = 0.959294497566666

5 1 1.5 2 2.5 3 3.5 4 4.5

2)

8 10 12 14 16
min)

(2)

8 10 12 14 16
(min)

(2)
 8 10 12 14 16
(min)
pH 0 10 slope pH slope
3.5 Trial 1 0.0684 0.0867 0.00183 3.5 0.00183
3.5 Trial 2 0.0708 0.0868 0.0016 3.5 0.0016
5.5 Trial 1 0.176 0.4063 0.02303 5.5 0.02303
p
5.5 Trial 2 0.156 0.3182 0.01622 5.5 0.01622
7.5 Trial 1 0.2854 1.3838 0.10984 7.5 0.10984 0.12

7.5 Trial 2 0.2173 1.2508 0.10335 7.5 0.10335 0.1

what is -1/-2? Idk lol 0.08

run 1 run 2 0.06

V0
0.04

0.02

0
3 3.5 4 4.5

pH 3.5 Assay 1
0.1 0.1
0.09 0.09
0.08 0.08
Absorbance (401nm)

Absorbance (401nm)
0.07 0.07
0.06 0.06
0.05 0.05
0.04 0.04
0.03 0.03
0.02 0.02
0.01 0.01
0 0
0 2 4 6 8 10 12 0
Time (mins)

pH 5.5 Assay 1
0.45 0.35
0.4
0.3
0.35
Absorbance (401nm)

Absorbance (401nm)

0.3 0.25

0.25 0.2
0.2 0.15
0.15
0.1
0.1
0.05 0.05
0 0
0 2 4 6 8 10 12 0
Time (mins)
 0.25 0.2

Absorbance (
Absorbance
0.2 0.15
0.15
0.1
0.1
0.05 0.05
0 0
0 2 4 6 8 10 12 0
Time (mins)

pH 7.5 Assay 1
1.6 1.4
1.4 1.2
1.2

Absorbance (401nm)
Absorbance (401nm)

1
1
0.8
0.8
0.6
0.6
0.4
0.4
0.2 0.2

0 0
0 2 4 6 8 10 12 0
Time (mins)

3.5 -1
0.1
0.09
0.08 f(x) = 0.0183 x + 0.0501
R² = 1
0.07
0.06
0.05
0.04
0.03
0.02
0.01
0
0.8 1 1.2 1.4 1.6 1.8 2 2.2

5.5-1
0.45 0.35
0.4
f(x) = 0.2303 x − 0.0543 0.3
0.35 R² = 1
 5.5-1
0.45 0.35
0.4
f(x) = 0.2303 x − 0.0543 0.3
0.35 R² = 1
0.25
0.3
0.25 0.2

0.2 0.15
0.15
0.1
0.1
0.05 0.05

0 0
0.8 1 1.2 1.4 1.6 1.8 2 2.2 0.8 1

7.5-1
1.4
1.6
1.2 f
1.4
f(x) = 1.0984 x − 0.813 R
1.2 R² = 1 1

1 0.8

0.8 0.6
0.6
0.4
0.4
0.2
0.2
0
0 0.8 1
0.8 1 1.2 1.4 1.6 1.8 2 2.2
 pH vs. V0
0.12

0.1

0.08

0.06

0.04

0.02

0
3 3.5 4 4.5 5 5.5 6 6.5 7 7.5 8
pH

pH 3.5 Assay 2
0.1
0.09
0.08
Absorbance (401nm)

0.07
0.06
0.05
0.04
0.03
0.02
0.01
0
0 2 4 6 8 10 12
Time (mins)

pH 5.5 Assay 2
0.35

0.3
Absorbance (401nm)

0.25

0.2

0.15

0.1

0.05

0
0 2 4 6 8 10 12
Time (mins)
 0.2

Absorbance (
0.15

0.1

0.05

0
0 2 4 6 8 10 12
Time (mins)

pH 7.5 Assay 2
1.4

1.2
Absorbance (401nm)

0.8

0.6

0.4

0.2

0
0 2 4 6 8 10 12
Time (mins)

3.5-2
0.1
0.09
0.08 f(x) = 0.016 x + 0.0548
R² = 1
0.07
0.06
0.05
0.04
0.03
0.02
0.01
0
0.8 1 1.2 1.4 1.6 1.8 2 2.2

5.5-2
0.35

0.3 f(x) = 0.1622 x − 0.00619999999999998

R² = 1
 5.5-2
0.35

0.3 f(x) = 0.1622 x − 0.00619999999999998

R² = 1
0.25

0.2

0.15

0.1

0.05

0
0.8 1 1.2 1.4 1.6 1.8 2 2.2

7.5 -2
1.4

1.2 f(x) = 1.0335 x − 0.8162

R² = 1
1

0.8

0.6

0.4

0.2

0
0.8 1 1.2 1.4 1.6 1.8 2 2.2
Temp 0.0000001 15 Slope
50 0.1731 0.3548 0.012113
60 0.1813 0.3393 0.869565 Temperature vs V0
70 0.1637 0.257 0.590506
1
0.9
0.8
0.7

V0 (mmol/min)
Temperature vs time 0.6
Temp 1E-07 15 Slope 0.5
50.000001 0.1731 0.3548 0.012113 0.4
60 0.1813 0.3393 0.869565 0.3
0.2
70 0.1637 0.257 0.590506
0.1
0
50 55 60 65
Temperature (°C)

Thermal Inactivati
0.4
0.35
f(x) = 0.0121133334140889 x + 0.1730
0.3 R² = 1
Absorbance (401mn)

0.25
0.2
0.15
0.1
0.05
0
0 2 4 6 8
Time (m

Thermal Inactivati
0.3

0.25
f(x) = 0.00622000004146667 x + 0.1
Absorbance (401mn)

R² = 1
0.2

0.15

0.1

0.05
 0.25
f(x) = 0.00622000004146667 x + 0.1

Absorbance (401mn)
R² = 1
0.2

0.15

0.1

0.05

0
0 2 4 6 8
Time (m
emperature vs V0

60 65 70 75
Temperature (°C)

Thermal Inactivation (50°C) Thermal Inactivation (60°C)

0.4
0.35
= 0.0121133334140889 x + 0.173099998788667
1 0.3 f(x) = 0.0105333334035556 x + 0.181299998946667
Absorbance (401mn)

R² = 1
0.25
0.2
0.15
0.1
0.05
0
2 4 6 8 10 12 14 16 0 2 4 6 8 10 12
Time (min) Time (min)

Thermal Inactivation (70°C)

= 0.00622000004146667 x + 0.163699999378
1
= 0.00622000004146667 x + 0.163699999378
1

2 4 6 8 10 12 14 16
Time (min)
ctivation (60°C)

6 x + 0.181299998946667

8 10 12 14 16
Time (min)
zoom out for more charts

a=ebc
Inhibition (18μL) 0 10 substrate conc abs/molar=conc slope 1/[s]
0 0.0189 0.014 0 7.61697497E-07 -0.00049
50 0.0265 0.0528 0.1375 2.8726877E-06 0.00263 7.272727
80 0.0255 0.078 0.22 4.2437432E-06 0.00525 4.545455
120 0.0445 0.0962 0.33 5.23394995E-06 0.00517 3.030303
170 0.0469 0.1596 0.4675 8.68335147E-06 0.01127 2.139037
230 0.0769 0.1287 0.6325 7.00217628E-06 0.00518 1.581028
300 0.0936 0.1898 0.825 1.03264418E-05 0.00962 1.212121
380 0.0734 0.0259 1.045 1.40914037E-06 -0.00475
470 0.0986 0.2418 1.2925 1.31556039E-05 0.01432 0.773694
570 0.1308 0.3383 1.5675 1.8405876E-05 0.02075 0.637959
680 0.1135 0.2776 1.87 1.51033732E-05 0.01641 0.534759
800 0.1339 0.2506 2.2 1.36343852E-05 0.01167 0.454545

18 μL inhibiton slope substate conc slope

50 0.00263 0.1375 0.00263 Lineweaver-Burk 18µL Inhibit
80 0.00525 0.22 0.00525 400000
120 0.00517 0.33 0.00517 350000
f(x) = 41974.635317461 x + 46845.4080396346
170 0.01127 0.4675 0.01127 300000
230 0.00518 0.6325 0.00518 250000
1/[Product]

300 0.00962 0.825 0.00962 200000

470 0.01432 1.2925 0.01432
150000
570 0.02075 1.5675 0.02075
100000
680 0.01641 1.87 0.01641
50000
800 0.01167 2.2 0.01167
0
0 1 2 3 4
1/[S]

slope
0.025

0.02

0.015

0.01

0.005

0
0 100 200 300 400 500 600 700 800 900
-0.005

-0.01
 0
0 100 200 300 400 500 600 700 800 900
-0.005

-0.01

18µL Inhibitor Assay - Michaelis-Menten

0.025

0.02
V0 (mmol/s)

0.015 f(x) = 0.00505092204708679 ln(x) − 0.0175855361538187

R² = 0.677928667447971
0.01

0.005

0
0 100 200 300 400 500 600 700 800 900
Axis Title

18µL Inhibitor Assay - Michaelis-Menten

0.025

0.02
V0 (mmol/s)

0.015 f(x) = 0.00505092204708679 ln(x) + 0.0121954799330224

0.01

0.005

0
0 0.5 1 1.5 2 2.5
[S] (mM)
 1/[product] Inhibition (6μL) 0 10 substrate c abs/molar=conc
0 = DNE 0 0
348106.1 50 0.0984 0.266 0.1375 1.4472252E-05
235641 80 0.1146 0.3006 0.22 1.6354733E-05
191060.3 120 0.1198 0.3742 0.33 2.0359086E-05
115162.9 170 0.1232 0.4001 0.4675 2.1768226E-05
142812.7 230 0.1297 0.5937 0.6325 3.2301415E-05
96838.78 300 0.1546 0.6454 0.825 3.5114255E-05
380 0.1646 0.7159 1.045 3.8949946E-05
76013.23 470 0.1701 1.0499 1.2925 5.7121872E-05
54330.48 570 0.2406 0.7823 1.5675 4.2562568E-05
66210.37 680 0.1951 0.9245 1.87 5.0299238E-05
73343.97 800 0.2663 0.8921 2.2 4.8536453E-05

Lineweaver-Burk 6µL Inhibitor As

weaver-Burk 18µL Inhibitor Assay
Lineweaver-Burk
0.1 6µL
0.09
Inhibitor Assay
0.08
974.635317461 x + 46845.4080396346 50000
1/[Product]

0.07
f(x) = 0.0223923291036026
f(x) = 14608.4191141795 ln(x) + 0.054823025273403
x + 11428.7127283005
V0 (mmol/s)

0.06
0
0.05
0 0.5 1 1.5 2 2.5
0.04
1/[S]
0.03
0.02
0.01
0
0 0.5 1 1.5
2 3 4 5 6 7 8
[S] (mM)
1/[S]

Combined 18µL, 6
1/[s] 18ul 6ul 0ul
7.272727 348106.060606 69097.74 86494.118
400000
4.545455 235641.025641 61144.38 61553.918 350000
3.030303 191060.29106 49118.12 46122.961 f(x) = 41974.635317461 x + 46845.40
300000
2.139037 115162.907268 45938.52 20015.246 250000
1.581028 142812.742813 30958.4 96889.826 200000
1.212121 96838.7776607 28478.46 9132.0117 150000
0.956938 25673.98 84466.912 100000
0.773694 76013.2340778 17506.43 9432.8971 50000 f(x) = 7990.99441163438
4404.08239750869 x + 18817.7
44313.5
0.637959 54330.475909 23494.82 39006.791 0
0 1 2 3

18ul
Linear (6ul)
 150000
100000
50000 f(x) = 7990.99441163438
4404.08239750869 x + 18817.7
44313.5
0
0 1 2 3
0.534759 66210.3746398 19881.02 91991.992
0.454545 73343.9744613 20603.07 44246.509 18ul
Linear (6ul)

6µL Inhibitor Assay - Michaelis

0.1
0.09
0.08
0.07
f(x) = 0.0223923291036026 ln(x) − 0.077205603

V0 (mmol/s)
0.06
0.05
0.04
0.03
0.02
0.01
0
0 100 200 300 400 500
[S] (mM)
 0 10
slope 1/[s] 1/[product] 0µl inhibito 0 10 substrate c

0.01676 7.2727273 69097.744 50 0.0512 0.2125 0.1375

0.0186 4.5454545 61144.378 80 0.0669 0.2986 0.22
0.02544 3.030303 49118.119 120 0.1342 0.3985 0.33
0.02769 2.1390374 45938.515 170 0.8178 0.9183 0.4675
0.0464 1.5810277 30958.396 230 0.557 0.1897 0.6325
0.04908 1.2121212 28478.463 300 1.1908 2.0127 0.825
0.05513 0.9569378 25673.977 380 0.0984 0.2176 1.045
0.08798 0.7736944 17506.429 470 1.9003 1.9485 1.2925
0.05417 0.6379585 23494.823 570 0.1681 0.4712 1.5675
0.07294 0.5347594 19881.017 680 0.1497 0.1998 1.87
0.06258 0.4545455 20603.071 800 0.1742 0.4154 2.2

Burk 6µL Inhibitor Assay

Lineweaver-Burk 0µL Inhibitor A
120000

100000
6026 ln(x) + 0.054823025273403
80000
1/[Product]

f(x) = 4404.08239750869 x + 44313.5479535188

60000

40000

20000

1 1.5 2 2.5 0
0 1 2 3 4 5
[S] (mM)
1/[S]

Combined 18µL, 6µL, and 0µL Inhibitor Lineweaver- 0.1

0µL Inhibitor Assay - Michaelis
Burk Data
0.08

0.06
= 41974.635317461 x + 46845.4080396346
0.04
V0 (mmol/s)

0.02
f(x) = 0.000447638641795566 ln(x) + 0.01546473
0
0 100 200 300 400 500
= 7990.99441163438
4404.08239750869 x + 18817.7068833186
44313.5479535188
-0.02
1 2 3 4 5 6 7 8
-0.04
18ul Linear (18ul) 6ul
Linear (6ul) 0ul Linear (0ul) -0.06
[S] (mM)
 f(x) = 0.000447638641795566 ln(x) + 0.01546473

V0 (
0
0 100 200 300 400 500
= 7990.99441163438
4404.08239750869 x + 18817.7068833186
44313.5479535188
-0.02
1 2 3 4 5 6 7 8
-0.04
18ul Linear (18ul) 6ul
Linear (6ul) 0ul Linear (0ul) -0.06
[S] (mM)

0µL Inhibitor Assay - Michaelis-M

bitor Assay - Michaelis-Menten 0.1
0.08
0.06

V0 (mmol/s)
0.04
3291036026 ln(x) − 0.0772056037649042
0.02
f(x) = 0.000447638641795568 ln(x) + 0.0181040
0
0 0.5 1 1.5
-0.02
-0.04
-0.06
300 400 500 600 700 800 900 [S] (mM)
[S] (mM)
 0 µL Substrate a
0.02
0.018 f(x) = − 0.00049 x + 0.0189
abs/molar=conc slope 1/[s] 1/[product] 0.016

Absorbance (401nm)
0.014
0.012
1.156148E-05 0.01613 7.2727273 86494.118
0.01
1.6245919E-05 0.02317 4.5454545 61553.918 0.008
2.1681175E-05 0.02643 3.030303 46122.961 0.006
4.9961915E-05 0.01005 2.1390374 20015.246 0.004
1.0321001E-05 -0.03673 1.5810277 96889.826 0.002
0.0001095049 0.08219 1.2121212 9132.0117 0
0 2 4
1.1838955E-05 0.01192 0.9569378 84466.912
0.00010601197 0.00482 0.7736944 9432.8971
2.5636561E-05 0.03031 0.6379585 39006.791
1.0870511E-05 0.00501 0.5347594 91991.992
2.2600653E-05 0.02412 0.4545455 44246.509

weaver-Burk 0µL Inhibitor Assay

239750869 x + 44313.5479535188 80 µL Substrate a

0.09
10 min 6ul microl assay
0.08
0.1007
0.07
Absorbance (401nm)
0.1273
0.06
0.1369
0.05
2 3 4 5 6 7 8 0.2293
0.04
1/[S] 0.157
0.03
0.1939
0.02
0.2115
0.01
0.228
0
µL Inhibitor Assay - Michaelis-Menten 0.3434 0 2 4
0.2586
1.0447
170 µL Substrate
0.18
0.16
0.14
Absorbance (401nm)

0.000447638641795566 ln(x) + 0.0154647320411982 0.12

0.1
200 300 400 500 600 700 800 900
0.08
0.06
0.04
0.02
0
[S] (mM) 0 2 4
0.000447638641795566 ln(x) + 0.0154647320411982 0.12

Absorbance (401
0.1
200 300 400 500 600 700 800 900
0.08
0.06
0.04
0.02
0
[S] (mM) 0 2 4

Inhibitor Assay - Michaelis-Menten

300 µL Substrate
0.2
0.18
0.16

Absorbance (401nm)
0.14
0.000447638641795568 ln(x) + 0.018104078573995 0.12
0.5 1 1.5 2 2.5 0.1
0.08
0.06
0.04
0.02
[S] (mM) 0
0 2 4

470 µL Substrate
0.3

0.25
Absorbance (401nm)
0.2

0.15

0.1

0.05

0
0 2 4

680 µL Substrate a
0.3

0.25
Absorbance (401nm)

0.2

0.15

0.1

0.05
 0.25

Absorbance (401nm)
0.2

0.15

0.1

0.05

0
0 2 4
 0 µL Substrate and High Inhibitor (18µL)
0.02
0.018 f(x) = − 0.00049 x + 0.0189
0.016 50 µL Substrate and High Inhibi
0.014
0.06
0.012
0.01
0.05
0.008
0.006
0.04
0.004
0.002
0.03
0
0 2 4 6 8 10 12
0.02
Time (min)

0.01

0
0 2 4 6 8

120 µL Substrate and High Inhibi

0.12

Absorbance (401nm) 0.1

80 µL Substrate and High Inhibitor (18µL) 0.08

0.09 0.06
0.08
0.04
0.07
0.06 0.02
0.05
0
0.04 0 2 4 6
0.03
Axis Title
0.02
0.01
0
0 2 4 6 8 10 12
Axis Title

170 µL Substrate and High Inhibitor (18µL) 230 µL Substrate and High Inh
0.14
0.18
0.16 0.12
Absorbance (401nm)

0.14 0.1
0.12
0.08
0.1
0.08 0.06
0.06 0.04
0.04
0.02
0.02
0
0 0 2 4 6
0 2 4 6 8 10 12
Time (min) Time (min)
 0.12

Absorbance (40
0.08
0.1
0.08 0.06
0.06 0.04
0.04
0.02
0.02
0
0 0 2 4 6
0 2 4 6 8 10 12
Time (min) Time (min)

300 µL Substrate and High Inhibitor (18µL) 380 µL Substrate and High Inh
0.2
0.08
0.18
0.16 0.07
0.14 0.06

Absorbance (401nm)
0.12 0.05
0.1
0.04
0.08
0.06 0.03
0.04 0.02
0.02 0.01
0
0 2 4 6 8 10 12 0
0 2 4 6
Time (min)
Time (min)

470 µL Substrate and High Inhibitor (18µL) 570 µL Substrate and High Inh
0.4
0.3
0.35
0.25
0.3
Absorbance (401nm)

0.2 0.25
0.2
0.15
0.15
0.1
0.1
0.05 0.05
0
0 0 2 4 6
0 2 4 6 8 10 12
Time (min)
Time (min)

680 µL Substrate and High Inhibitor (18µL) 800 µL Substrate and High Inhib
0.3
0.3
0.25
0.25
Absorbance (401nm)

0.2
0.2
0.15
0.15
0.1
0.1
0.05
0.05
 0.3
0.25
0.25

Absorbance (401nm)
0.2
0.2
0.15
0.15
0.1
0.1
0.05
0.05
0
0 2 4 6 8 10 12 0
0 2 4 6
Time (min)
Time (min)
ubstrate and High Inhibitor (18µL)

4 6 8 10 12

ubstrate and High Inhibitor (18µL)

4 6 8 10 12
Axis Title

Substrate and High Inhibitor (18µL)

2 4 6 8 10 12
Time (min)
 2 4 6 8 10 12
Time (min)

L Substrate and High Inhibitor (18µL)

2 4 6 8 10 12
Time (min)

L Substrate and High Inhibitor (18µL)

2 4 6 8 10 12
Time (min)

Substrate and High Inhibitor (18µL)

4 6 8 10 12
Time (min)
microliters 0 10 dead substrate conc abs/molar=conc 1/[s] 1/conc
0 -0.0299 0.1029 0.1029 0 5.59847661E-06
50 0.1293 0.266 0.266 0.0001375 1.44722524E-05 7272.727 69097.74
80 - 0.3006 0.3006 0.00022 1.63547334E-05 4545.455 61144.38
120 0.3056 0.3742 0.3742 0.00033 2.0359086E-05 3030.303 49118.12
170 0.3792 0.4001 0.4001 0.0004675 2.17682263E-05 2139.037 45938.52
230 0.5355 0.5937 0.5937 0.0006325 3.23014146E-05 1581.028 30958.4
300 0.5773 0.6454 0.6454 0.000825 3.51142546E-05 1212.121 28478.46
380 0.7246 0.7159 0.7159 0.001045 3.89499456E-05 956.9378 25673.98
470 0.7225 1.0499 1.0499 0.0012925 5.71218716E-05 773.6944 17506.43
570 0.8739 0.7823 0.7823 0.0015675 4.2562568E-05 637.9585 23494.82
680 0.9935 0.9245 0.9245 0.00187 5.02992383E-05 534.7594 19881.02
800 1.039 0.8921 0.8921 0.0022 4.85364527E-05 454.5455 20603.07

Michaelis-Menten for 6ul

0.00006

0.00005

0.00004

0.00003

0.00002

0.00001

0
0 0.0005 0.001 0.0015 0.002 0.0025

Lineweaver-Burk Plot
80000

70000

60000

50000

40000

30000

20000

10000

0
0 1000 2000 3000 4000 5000 6000 7000 8000
slope
0.01328
0.01367
0
0.00686
0.00209
0.00582
0.00681
-0.00087
slope
0.03274 0.04
-0.00916
-0.0069 0.03

-0.01469
0.02

0.01

prob not right 0

0 100 200 300 400 500 600 700 800 900

-0.01

-0.02

prob not right