oN OF PHTHALATES ESTERS AND ACRYLAMIDE IN f i i pthalate esters of plastic packages in food samples from plastic food of phthalates packages in a f a = sateen bale food sample is determined by injection of mie pli phy with FID detection, Sample is extracted with ie ae acid and Icad acetate for different foods and then analyzed by GC-FID. 9" : ’ atv ¢ 5 conti machine * uasoiestor as Chromatography- Flame lonization Detector «paste botles of PET, HDPE and PVC (500 mi) «LDPE bags eagets ‘+ 16% tricholoroacetie acid solution + 4% lead acetate solution + 5% sodium chloride solution © Distilled water © Ethanol + GC grade methanol © Hexane Samples © Water, juice, milk © Chicken curry eneSECTION III k solution: f standard stoc Preparation o © Prepare indard - he concentration of stock solution prior to working limits with met e the con * Decrease the co ing standard solution from 1000ppm stock solution Containing Polya working ing | hy, thano} in GC vial by auto sampler ce Il of working solution it * Introduce Ip! of Procedure fhe 500m1 plastic bottles of Polyethylene terephthalate ( PET), High density Poi, © Use the s " (HDPE) and polyvinyl chloride (PVC) to store milk, juice and water lene Store the chicken curry in low density polyethylene (LDPE) bags, For milk, juice and chicken curry * Mix 30g of sample with Sml of trichloroacetic acid solution followed by cent in 4000rpm for 5 minutes, * Dissolve the supernatants with 4ml of lead acetate solution, * Do the ultracentrifugation at 4000rpm for 5 minutes for the Separation of, Organic phase, * Combine 8m! of organic phase with sodium chloride solution in 10ml Centrifuge tube, ¢ Add 0.8m! of methanol and 40u1 of carbon tetrachloride in solution and Shake for few Secon physically * Blend the solution in ultrasonication bath at 40 kHz for 5 minutes * Introduce 111 of extract into vial of GC by auto sampler for further analysis For water Take 10ml of sample in vial afterwards add 20u! of methanol and 500,11 of hexane followed the formation of a cloudy solution, . Place it in ultrasonication bath for 5 minutes to obtain the Organic phase, * Int troduce the Iu} of sample into GC by auto sampler for further analysis.Comp) NSIVE AN METHODS REH T ENS, SIVE ANALYTICAL A ETHODS Flame ionuan aii nization — Helium at m/s | At operational veloc a ity of 30c1 Splitless [al oe 90Cfor2minutes SOSOSOSC~S | eon Increase at the rate of 20°C/min to 190°C © 15°C/min to 210°C | © 10°C/min to 290°C Lemons tae a Sf oo | sow ate (oF FID) FP 3000nirmin ‘ers ey Towrae —Haimin quid microextraction lowed by 28S 3: Reference d-based disperser liguid-Ii ate fol Separation Science, farajzadeh, M.A., Pp. Khorram and A.A. Nabil. 2014. Soli alate esters and di-(2-ethylhexyl) adips spectrometry. Journal of ncentration of phth: for the precot n detection oF mass romatography with flame jonizatio 1177-1184.