9/1/23, 23:13 Comparison of two PCR and one ELISA techniques for the detection of small ruminant lentiviruses

iviruses (SRLVs) in milk of sheep and goats…

Research in Veterinary Science

Volume 94, Issue 3, June 2013, Pages 817-819

Comparison of two PCR and one ELISA techniques for the detection of small
ruminant lentiviruses (SRLVs) in milk of sheep and goats
N. Barquero , A. Domenech, A. Arjona, J.F. Fernández-Garayzabal, J.A. Ruiz-Santa-Quiteria, E. Gomez-Lucia

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Abstract

The aim of the present study was to compare the efficiency of two PCR techniques for the diagnosis of small ruminant
lentiviruses (SRLVs). Detection of the proviral genome by PCR, though sensitive, is difficult due to the heterogeneity of the
SRLV genomes. One of the PCR techniques amplifies a fragment in the pol gene (pol-PCR) and the other PCR targets the LTR
region of the proviral genome (LTR-PCR). Milk from 194 sheep and 163 goats from farms in the Central Spain was analyzed
by both techniques and compared to results obtained by ELISA. When compared to the serologic assay, the agreement of
both PCR techniques was very low (0.024 and 0.020 in sheep, and 0.124 and 0.114 in goats). In view of these results, it may be
concluded that the efficacy of PCR for the diagnosis of SRLVs is low and a combination of PCR and ELISA should be used
for diagnosis.

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Acknowledgements
The authors are thankful to the Cooperativa de Ganaderos de Castilla La Mancha for their assistance in obtaining samples,
to Cristina Toural for her technical assistance, and to Alfonso Las Heras for his collaboration in collecting samples.…

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There are more references available in the full text version of this article.

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…In this context, several other studies have successfully demonstrated the use of PCR technique to detect pro-viral DNA of SRLV,15–
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