SCIENCE G7 QUARTER 2

MICROSCOPY

1. Magnification is a measure of the ability of a lens to magnify.

2. An object of any size, if viewed under the microscope, is magnified to form an enlarged image.

3. The magnification of a specimen can be calculated by multiplying the number found in the
eyepiece with the number found on the objective used for focusing. Hence, the formula is:
TOTAL Magnification of the specimen = Magnification of the eyepiece x Magnification of the
objective
Magnification of eyepiece = Total Magnification of specimen ÷ Magnification of objective
Magnification of objective = Total Magnification of specimen ÷ Magnification of eyepiece

4. Eyepiece lenses of different microscopes may have different magnifications.

5. The LPO and HPO has different magnifications which results to the different magnifications of
specimen

A wet mount is a prepared slide with a drop of water to suspend the specimen between the slide
and the cover slip.

The wet mount or any mount preparation is important because the specimen is the most important
material in order to study objects using the microscope.

Do’s and the Don’ts in Preparing a Wet Mount

a. Avoid touching the cover glass area, slides should be held by the edges.
b. Add only one drop of clean water over the specimen.
c. Excess water should be wiped with tissue paper.
d. Use a sharp blade or scalpel in extracting a specimen to avoid damage in it.
e. Do not apply pressure on the cover glass it can cause breakage.
f. Wash the soiled slides with soapy water or isopropyl alcohol.

1. Cut out a small letter “e” from the newspaper page

2. Using forceps or tweezers place the cut out in the center of a glass slide with the printed side up.
3. Using a dropper, add a drop of clean water over the specimen.
4. Position the cover slip at 45 ֯ with one side touching one edge of the water on the slide.
5. Slowly lower the other edge of the cover slip until it rests on the water and on the piece of
newspaper cut out.
6. Make the bubble move towards the edge of the cover slip by tapping it with the eraser-end of a
pencil.
7. Focus the specimen under the microscope using the scanning objective.
8. Draw the specimen as viewed under the microscope