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  • LS 2202, Lecture 12

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    Akshat Misra
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    The document discusses properties of DNA polymerases including that they add nucleotides in the 5' to 3' direction, require a template and primer, are extremely accurate with proofreading activity, and replicate DNA quickly due to being processive. It also mentions telomeres which protect the ends of chromosomes, the end replication problem, and that telomerase is a ribonucleoprotein containing an RNA component and reverse transcriptase protein that functions as an RNA-dependent DNA polymerase to extend telomeres and regulate replication in eukaryotes.

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    The document discusses properties of DNA polymerases including that they add nucleotides in the 5' to 3' direction, require a template and primer, are extremely accurate with proofreading activity, and replicate DNA quickly due to being processive. It also mentions telomeres which protect the ends of chromosomes, the end replication problem, and that telomerase is a ribonucleoprotein containing an RNA component and reverse transcriptase protein that functions as an RNA-dependent DNA polymerase to extend telomeres and regulate replication in eukaryotes.

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    © All Rights Reserved
    Download as PPSX, PDF, TXT or read online from Scribd
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    3 views11 pages

    LS 2202, Lecture 12

    Uploaded by

    Akshat Misra
    The document discusses properties of DNA polymerases including that they add nucleotides in the 5' to 3' direction, require a template and primer, are extremely accurate with proofreading activity, and replicate DNA quickly due to being processive. It also mentions telomeres which protect the ends of chromosomes, the end replication problem, and that telomerase is a ribonucleoprotein containing an RNA component and reverse transcriptase protein that functions as an RNA-dependent DNA polymerase to extend telomeres and regulate replication in eukaryotes.

    Copyright:

    © All Rights Reserved
    Download as PPSX, PDF, TXT or read online from Scribd
    Flag for inappropriate content
    of 11

    Lecture - 12

    Properties of DNA Polymerases

    • Adds nucleotides in 5’ to 3’ direction

    • Requires a template and a primer

    • Extremely accurate

    • Also has proof-reading activity to correct errors

    • Very fast reaction due to processive nature


    Replication at chromosome ends

    • In humans the telomere sequence is


    TTAGGG. This sequence is usually
    repeated several thousand times
    • Telomeric proteins protects the
    chromosomal ends from damage
    End replication problem
    Telomerase: A ribonucleoprotein

    TERT: Telomerase reverse transcriptase


    TER: Telomerase RNA
    Annu Rev. Biochem. 1996

    Telomerase: A RNA dependent


    DNA polymerase
    Extension of the 5’ end of the chromosome
    Regulation of replication
    Bacteria Eukaryotes
    Helicase loading and activation
    Fundamentals of Cell Cycle Regulation
    Regulated helicase loading
    and activation

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