CALCIUM KIT (OGPC method) For be dloirton of Calum sau o patna ori Dagon Us Oh) Summary Calcum, the body, is found main in the bones (approxrate 92%). n sou cakium ess equaly na Irogneedformanginaboune ‘am wih burn Hence 2 cacreaaeinsbumincauresloweralcum ves and ies versa Tho lvls of Caloum serv Spondon Be faramyridhomoreIezeesodCalsumlovlsae foun Bane uous, hyperparayridsn. Deceased aes ae found in hypoperathyreiis, eal flue, res, iin Deatcinayndpancreatit Principe Calcium in an alkaline medium combines with © Creslpihalen Conlexoe to fom 8 purl xoured temple. intrsty of the colour fomed is det Popotonal ithe ancurtofcalcum resantinbe sap. Calcium + 0c? Purple coloured complex Normal reference values SerumiPasma a7-11omgia I's resoniranded tate rralrangereproseting Iapratory els sown ertpopain, Contents axasmb 2x75 24150 LB ane Sm Trl 80m Si CalionSaniae tors) Sm Sel Em Storage stably Connie tala 2-8 °C the expiry mentored on telat Reagent Preparation Reagens are ready o use. rate! tom bight ight Working Reagent: For convenience a snge working ‘eagortmaybe proparedby mang oa parts fhe Bue Reagent ard Colour Reagent This combined reagent i stablfor7 days at 2-8°0 Sample material Sorum / Hepaized plasma, Calbum i opr to be stalin sermforToaysat28'c Procedure Wavelangh filer sTonm(gS78am)/Ylow Terperaze RT Lapa ton Ppt ito clean cry lst bes abla 2s Blak (8), Standard), end Test 7 LT ‘tion Bos oT Sequence yeh ey ButerReagen(lty | 5 05 08: Cow Rasgent(2) | 05 05 as, Disled water one : Celsum Standard (8) a Samp one Mic wallandincabate at. 250} Sin Measure the absorbance of the Standart (Abs), and Test Sale (fos Tapas Bank witindoMin Caleulations Abst Cakiuninmgd = x 10 As Linearty This procedure islinear plo 18 mo. vals exe tr nt, ditto spl wih dstled wale and peat theory Calle th value using be prope lon fae cum’ vary wey stated ion, car tou be taken svis any conairatonAligassware tongues Forte osteo est be ined wi 135 0¢ 04 NCI ans enw goad quly dirs atrboforeuse, issues ata essing of bes wibHCl ibe reaget be posta ner respocve bes ard ibs Be nsec wih tho reagent The Teagont Sen shauld be pocls tage n Be ar tie an eipeted oto the'iandod ad ee We! es, The lena a any reninigcarainason lb cari ver equal al ‘ho ors Fe ow col euvtes fs suggested ta sme reagent be aprala bafra the Bink 1 ake away any tentarnsten he fou trough big or oe which rrayeauseaighorhan we setallankototeagent Chelating agers euch as EDTA, poset ove in tacts, prevent Ihe amaton of the coaur cmon, hence receesarcaresnoldbe ken cuingthe 53, Reterences (Geamen Ht, 1957), Anal Blochom.18:521 Baga, (1873) Cin Chom Aca 4646Reaction: End Pos Wavelength: s70nm Zero Seng: Reagent lank Ineub.Tomp. RT Ineub: Time Sin DelayTime : — ReadTime : = Noofresd. Inara = ‘Sample Vol. : Oc2mi Reagent Vol. 1.00 ‘Standard omg Frctor S React. lope: Inceasing Lnsaty ‘engl Unite pd cxsooreaey ‘tana Dr. Arno Do Rego Bagh Bamboim Gorpox 20. GOA. 403202, NDIA