MICROBIO RD - ASEPTIC TRANSFER AND STREAK METHOD

Aseptic transfer from culture media to agar plate

Materials:
Inoculating loop
1 petri dish (clear gulaman) label as your culture media
1 petri dish (red gulaman) label as your agar plate
Alcohol lamp
Clean gloves

Procedure:
1. Sterilize inoculating loop (note: dapat mag -oorange sya)
2. Wait until macool down ng konti (note: wag agad ilagay sa gelatin kasi baka masira
[minus pts yon] )
3. Pretend lang na kumukuha ka ng bacteria sa culture media
4. Takpan agad
5. Open the agar plate (red gulaman) then gawin na yung streak method.

In doing streak method: https://www.youtube.com/watch?v=pfrjpyZ-Wuw

1. Sa isang side muna i-slide yung inoculating loop

2. Takpan yung petri dish
3. Sterilize inoculating loop
4. Wait until macool down ng konti
5. Slide mo ulit sa isang side naman
6. Then ulit lang hanggang sa pang apat na slide na
Note: agad takpan yung petri dish. Watch nyo vid makakatulong yan.

Aseptic transfer from broth to agar slant

Materials:
Inoculating loop
1 test tube with water as your broth w/ cap
1 test tube as your agar w/ cap
Alcohol lamp
Clean gloves

Procedure: https://www.youtube.com/watch?v=goM1sby7wu4
1. Nasa yt na procedure
Note: basta lagi kayo mag sterilize ng loop and ng test tube
Wag nyo tamaan yung tube pag kumukuha sa broth.