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Support Absorbance to Transmittance Converter

Absorbance to Transmittance Converter

Use the following calculator to convert absorbance to transmittance values and vice versa. A
transmittance to absorbance reference table of values is presented below.

Thousands Separator 1,000 1'000

Absorbance <=> Transmittance

WHAT IS THE BEER LAMBERT LAW?

The Beer Lambert law, which is also referred to as Beer’s Law, describes the relationship among
absorbance (A), the molar solute concentration in M (c), and the length of the path the light takes to
get to the sample in centimeters (l). Absorbance is directly proportional to concentration and length:
A = εcl. ε is the wavelength-dependent molar absorbtivity coef cient and it is constant for a
particular substance. ε has units of L mol – 1 cm – 1 . The Beer’s law provides a linear relationship
between concentration and absorbance that can be plotted to produce an easy-to-use graph.

Beer Lambert Calculator

calculate calculate calculate calculate

= x x 0
0 0 0
Absorbance A Molar absorbtivity Path length Concentration
Extinction (cm) (mol/L)
cof cient

(l mol-1 cm-1)
TRANSMITTANCE TO ABSORBANCE TABLE
Absorbance and transmittance are measurements used in spectrophotometry. Spectrophotometry
measures how much radiant energy a substance absorbs at varying wavelengths of light. The
technique is useful for determining the identity of an unknown substance as and, with the use of a set
of standards, determining a substance’s concentration in a sample.

A transmittance to absorbance table enables fast conversion from transmittance values to

absorbance in the lab or in the eld.

Transmittance Absorbance (AU)

100% 0
99% 0.004
98% 0.009
97% 0.013
96% 0.018
95% 0.022
90% 0.046
85% 0.071
80% 0.097
75% 0.125
70% 0.155
65% 0.187
60% 0.222
55% 0.26
50% 0.301
45% 0.347
40% 0.398
35% 0.456
30% 0.523
25% 0.602
20% 0.699
15% 0.824
10% 1
5% 1.301
1% 2
HOW IS ABSORBANCE CALCULATED FROM TRANSMITTANCE?
Absorbance can be calculated from percent transmittance (%T) using this formula:

Absorbance = 2 – log(%T)

Transmittance (T) is the fraction of incident light which is transmitted. In other words, it’s the amount
of light that “successfully” passes through the substance and comes out the other side. It is de ned as
T = I/Io, where I = transmitted light (“output”) and Io = incident light (“input”). %T is merely (I/Io) x
100. For example, if T = 0.25, then %T = 25%. A %T of 25% would indicate that 25% of the light
passed through the sample and emerged on the other side. Absorbance (A) is the ip-side of
transmittance and states how much of the light the sample absorbed. It is also referred to as “optical
density.” Absorbance is calculated as a logarithmic function of T: A = log10 (1/T) = log10 (Io/I).

Absorbance to transmittance can also be determined using th3 calculator above.

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